Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites

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Bibliographische Detailangaben
Zeitschriftentitel:	Bioinformatics
Personen und Körperschaften:	Park, Jeongbin, Bae, Sangsu, Kim, Jin-Soo
In:	Bioinformatics, 31, 2015, 24, S. 4014-4016
Format:	E-Article
Sprache:	Englisch
veröffentlicht:	Oxford University Press (OUP)
Schlagwörter:	Computational Mathematics Computational Theory and Mathematics Computer Science Applications Molecular Biology Biochemistry Statistics and Probability

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Zusammenfassung:	<jats:title>Abstract</jats:title> <jats:p>Summary: We present Cas-Designer, a user-friendly program to aid researchers in choosing appropriate target sites in a gene of interest for type II CRISPR/Cas-derived RNA-guided endonucleases, which are now widely used for biomedical research and biotechnology. Cas-Designer rapidly provides the list of all possible guide RNA sequences in a given input DNA sequence and their potential off-target sites including bulge-type sites in a genome of choice. In addition, the program assigns an out-of-frame score to each target site to help users choose appropriate sites for gene knockout. Cas-Designer shows the results in an interactive table and provides user-friendly filter functions.</jats:p> <jats:p>Availability and implementation: Free access at http://rgenome.net/cas-designer/.</jats:p> <jats:p>Contact: sangsubae@hanyang.ac.kr or jskim01@snu.ac.kr</jats:p> <jats:p>Supplementary information: Supplementary data are available at Bioinformatics online.</jats:p>
Umfang:	4014-4016
ISSN:	1367-4811 1367-4803
DOI:	10.1093/bioinformatics/btv537