Genome-wide modeling of transcription kinetics reveals patterns of RNA production delays

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Bibliographische Detailangaben
Zeitschriftentitel:	Proceedings of the National Academy of Sciences
Personen und Körperschaften:	Honkela, Antti, Peltonen, Jaakko, Topa, Hande, Charapitsa, Iryna, Matarese, Filomena, Grote, Korbinian, Stunnenberg, Hendrik G., Reid, George, Lawrence, Neil D., Rattray, Magnus
In:	Proceedings of the National Academy of Sciences, 112, 2015, 42, S. 13115-13120
Format:	E-Article
Sprache:	Englisch
veröffentlicht:	Proceedings of the National Academy of Sciences
Schlagwörter:	Multidisciplinary

Details
Zusammenfassung:	<jats:title>Significance</jats:title> <jats:p>Gene transcription is a highly regulated dynamic process. Delays in transcription have important consequences on dynamics of gene expression and consequently on downstream biological function. We model temporal dynamics of transcription using genome-wide time course data measuring transcriptional activity and mRNA concentration. We find a significant number of genes exhibit a long RNA processing delay between transcription termination and mRNA production. These long processing delays are more common for short genes, which would otherwise be expected to transcribe most rapidly. The distribution of intronic reads suggests that these delays are required for splicing to be completed. Understanding such delays is essential for understanding how a rapid cellular response is regulated.</jats:p>
Umfang:	13115-13120
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.1420404112