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Serum of patients with acute myocardial infarction prevents inflammation in iPSC-cardiomyocytes
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| Veröffentlicht in: | Scientific reports 9(2019) Artikel-Nummer 5651, 13 Seiten |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , , , , , , , |
| Titel: | Serum of patients with acute myocardial infarction prevents inflammation in iPSC-cardiomyocytes/ Katherine Sattler, Ibrahim El-Battrawy, Zhihan Zhao, Christoph Schrottenberg, Gökhan Yücel, Huan Lan, Xin Li, Siegfried Lang, Wolfram-Hubertus Zimmermann, Lukas Cyganek, Jochen Utikal, Thomas Wieland, Ursula Ravens, Karen Bieback, Martin Borggrefe, Xiaobo Zhou & Ibrahim Akin |
| Format: | E-Book-Kapitel |
| Sprache: | Englisch |
| veröffentlicht: |
04 April 2019
|
| Gesamtaufnahme: |
: Scientific reports, 9(2019) Artikel-Nummer 5651, 13 Seiten
, volume:9 |
| Quelle: | Verbunddaten SWB Lizenzfreie Online-Ressourcen |
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| contents | Acute myocardial infarction (MI) evokes a systemic inflammatory response and locally the degradation of the necrotic tissue, followed by scar formation. The mechanisms for containment of the infarct zone are not studied well. The study aimed to examine the response of healthy cardiomyocytes to serum of patients with myocardial infarction. Human iPSC-cardiomyocytes (iPSC-CM) generated from two healthy donors were incubated with serum of patients with MI with and without ventricular fibrillation (VF) or of healthy controls. Different cell adhesion molecules were studied by flow cytometry and immunostaining. Cellular electrophysiology was studied by patch clamp. The cell adhesion molecules CD54/ICAM-1, CD58/LFA-3 and CD321/JAM-A were expressed on iPSC-CM within the plasma membrane. Incubation with serum of MI patients reduced the levels of expression of CD54/ICAM-1 and CD321/JAM-A by 15-20%. VF serum was less effective than serum of MI patients without VF. MI serum or VF serum did not affect resting potential, action potential duration or maximum depolarization velocity. Myocardial infarction serum exerts anti-inflammatory effects on healthy cardiomyocytes without affecting their electrical activity, thus helping to contain the infarct zone and to protect healthy tissue. Ventricular fibrillation during MI drives healthy cardiomyocytes towards a pro-inflammatory phenotype. |
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| spelling | Sattler, Katherine 1976- VerfasserIn (DE-588)129625884 (DE-627)475212509 (DE-576)297755153 aut, Serum of patients with acute myocardial infarction prevents inflammation in iPSC-cardiomyocytes Katherine Sattler, Ibrahim El-Battrawy, Zhihan Zhao, Christoph Schrottenberg, Gökhan Yücel, Huan Lan, Xin Li, Siegfried Lang, Wolfram-Hubertus Zimmermann, Lukas Cyganek, Jochen Utikal, Thomas Wieland, Ursula Ravens, Karen Bieback, Martin Borggrefe, Xiaobo Zhou & Ibrahim Akin, 04 April 2019, Text txt rdacontent, Computermedien c rdamedia, Online-Ressource cr rdacarrier, Gesehen am 22.05.2019, Acute myocardial infarction (MI) evokes a systemic inflammatory response and locally the degradation of the necrotic tissue, followed by scar formation. The mechanisms for containment of the infarct zone are not studied well. The study aimed to examine the response of healthy cardiomyocytes to serum of patients with myocardial infarction. Human iPSC-cardiomyocytes (iPSC-CM) generated from two healthy donors were incubated with serum of patients with MI with and without ventricular fibrillation (VF) or of healthy controls. Different cell adhesion molecules were studied by flow cytometry and immunostaining. Cellular electrophysiology was studied by patch clamp. The cell adhesion molecules CD54/ICAM-1, CD58/LFA-3 and CD321/JAM-A were expressed on iPSC-CM within the plasma membrane. Incubation with serum of MI patients reduced the levels of expression of CD54/ICAM-1 and CD321/JAM-A by 15-20%. VF serum was less effective than serum of MI patients without VF. MI serum or VF serum did not affect resting potential, action potential duration or maximum depolarization velocity. Myocardial infarction serum exerts anti-inflammatory effects on healthy cardiomyocytes without affecting their electrical activity, thus helping to contain the infarct zone and to protect healthy tissue. Ventricular fibrillation during MI drives healthy cardiomyocytes towards a pro-inflammatory phenotype., El-Battrawy, Ibrahim 1987- VerfasserIn (DE-588)1033436917 (DE-627)741219549 (DE-576)38084673X aut, Zhao, Zhihan 1987- VerfasserIn (DE-588)112352680X (DE-627)876729960 (DE-576)48177825X aut, Schrottenberg, Christoph VerfasserIn aut, Yücel, Gökhan 1985- VerfasserIn (DE-588)1123528063 (DE-627)876733259 (DE-576)481781145 aut, Lan, Huan VerfasserIn (DE-588)1138467820 (DE-627)896044157 (DE-576)49256826X aut, Li, Xin VerfasserIn aut, Lang, Siegfried 1955- VerfasserIn (DE-588)1026112613 (DE-627)726109290 (DE-576)371365473 aut, Zimmermann, Wolfram-Hubertus VerfasserIn aut, Cyganek, Lukas VerfasserIn aut, Utikal, Jochen 1974- VerfasserIn (DE-588)1026463750 (DE-627)726765015 (DE-576)371816580 aut, Wieland, Thomas 1960- VerfasserIn (DE-588)1033445908 (DE-627)741233215 (DE-576)381043339 aut, Ravens, Ursula VerfasserIn aut, Bieback, Karen 1972- VerfasserIn (DE-588)124509991 (DE-627)376254653 (DE-576)294206604 aut, Borggrefe, Martin VerfasserIn (DE-588)1025920546 (DE-627)725574232 (DE-576)370913426 aut, Zhou, Xiao-Bo 1958- VerfasserIn (DE-588)1117113744 (DE-627)871032457 (DE-576)478766742 aut, Akın, Ibrahim 1978- VerfasserIn (DE-588)132322293 (DE-627)521039010 (DE-576)299074366 aut, Enthalten in Scientific reports [London] : Macmillan Publishers Limited, part of Springer Nature, 2011 9(2019) Artikel-Nummer 5651, 13 Seiten Online-Ressource (DE-627)663366712 (DE-600)2615211-3 (DE-576)346641179 2045-2322 nnns, volume:9 year:2019, https://doi.org/10.1038/s41598-019-42079-z Verlag Resolving-System kostenfrei Volltext, https://www.nature.com/articles/s41598-019-42079-z Verlag kostenfrei Volltext, https://www.nature.com/articles/s41598-019-42079-z LFER, LFER 2019-05-29T00:00:00Z |
| spellingShingle | Sattler, Katherine, El-Battrawy, Ibrahim, Zhao, Zhihan, Schrottenberg, Christoph, Yücel, Gökhan, Lan, Huan, Li, Xin, Lang, Siegfried, Zimmermann, Wolfram-Hubertus, Cyganek, Lukas, Utikal, Jochen, Wieland, Thomas, Ravens, Ursula, Bieback, Karen, Borggrefe, Martin, Zhou, Xiao-Bo, Akın, Ibrahim, Serum of patients with acute myocardial infarction prevents inflammation in iPSC-cardiomyocytes, Acute myocardial infarction (MI) evokes a systemic inflammatory response and locally the degradation of the necrotic tissue, followed by scar formation. The mechanisms for containment of the infarct zone are not studied well. The study aimed to examine the response of healthy cardiomyocytes to serum of patients with myocardial infarction. Human iPSC-cardiomyocytes (iPSC-CM) generated from two healthy donors were incubated with serum of patients with MI with and without ventricular fibrillation (VF) or of healthy controls. Different cell adhesion molecules were studied by flow cytometry and immunostaining. Cellular electrophysiology was studied by patch clamp. The cell adhesion molecules CD54/ICAM-1, CD58/LFA-3 and CD321/JAM-A were expressed on iPSC-CM within the plasma membrane. Incubation with serum of MI patients reduced the levels of expression of CD54/ICAM-1 and CD321/JAM-A by 15-20%. VF serum was less effective than serum of MI patients without VF. MI serum or VF serum did not affect resting potential, action potential duration or maximum depolarization velocity. Myocardial infarction serum exerts anti-inflammatory effects on healthy cardiomyocytes without affecting their electrical activity, thus helping to contain the infarct zone and to protect healthy tissue. Ventricular fibrillation during MI drives healthy cardiomyocytes towards a pro-inflammatory phenotype. |
| title | Serum of patients with acute myocardial infarction prevents inflammation in iPSC-cardiomyocytes |
| title_auth | Serum of patients with acute myocardial infarction prevents inflammation in iPSC-cardiomyocytes |
| title_full | Serum of patients with acute myocardial infarction prevents inflammation in iPSC-cardiomyocytes Katherine Sattler, Ibrahim El-Battrawy, Zhihan Zhao, Christoph Schrottenberg, Gökhan Yücel, Huan Lan, Xin Li, Siegfried Lang, Wolfram-Hubertus Zimmermann, Lukas Cyganek, Jochen Utikal, Thomas Wieland, Ursula Ravens, Karen Bieback, Martin Borggrefe, Xiaobo Zhou & Ibrahim Akin |
| title_fullStr | Serum of patients with acute myocardial infarction prevents inflammation in iPSC-cardiomyocytes Katherine Sattler, Ibrahim El-Battrawy, Zhihan Zhao, Christoph Schrottenberg, Gökhan Yücel, Huan Lan, Xin Li, Siegfried Lang, Wolfram-Hubertus Zimmermann, Lukas Cyganek, Jochen Utikal, Thomas Wieland, Ursula Ravens, Karen Bieback, Martin Borggrefe, Xiaobo Zhou & Ibrahim Akin |
| title_full_unstemmed | Serum of patients with acute myocardial infarction prevents inflammation in iPSC-cardiomyocytes Katherine Sattler, Ibrahim El-Battrawy, Zhihan Zhao, Christoph Schrottenberg, Gökhan Yücel, Huan Lan, Xin Li, Siegfried Lang, Wolfram-Hubertus Zimmermann, Lukas Cyganek, Jochen Utikal, Thomas Wieland, Ursula Ravens, Karen Bieback, Martin Borggrefe, Xiaobo Zhou & Ibrahim Akin |
| title_in_hierarchy | Serum of patients with acute myocardial infarction prevents inflammation in iPSC-cardiomyocytes / Katherine Sattler, Ibrahim El-Battrawy, Zhihan Zhao, Christoph Schrottenberg, Gökhan Yücel, Huan Lan, Xin Li, Siegfried Lang, Wolfram-Hubertus Zimmermann, Lukas Cyganek, Jochen Utikal, Thomas Wieland, Ursula Ravens, Karen Bieback, Martin Borggrefe, Xiaobo Zhou & Ibrahim Akin, |
| title_short | Serum of patients with acute myocardial infarction prevents inflammation in iPSC-cardiomyocytes |
| title_sort | serum of patients with acute myocardial infarction prevents inflammation in ipsc cardiomyocytes |
| url | https://doi.org/10.1038/s41598-019-42079-z, https://www.nature.com/articles/s41598-019-42079-z |