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Serum of patients with acute myocardial infarction prevents inflammation in iPSC-cardiomyocytes

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Veröffentlicht in: Scientific reports 9(2019) Artikel-Nummer 5651, 13 Seiten
Personen und Körperschaften: Sattler, Katherine (VerfasserIn), El-Battrawy, Ibrahim (VerfasserIn), Zhao, Zhihan (VerfasserIn), Schrottenberg, Christoph (VerfasserIn), Yücel, Gökhan (VerfasserIn), Lan, Huan (VerfasserIn), Li, Xin (VerfasserIn), Lang, Siegfried (VerfasserIn), Zimmermann, Wolfram-Hubertus (VerfasserIn), Cyganek, Lukas (VerfasserIn), Utikal, Jochen (VerfasserIn), Wieland, Thomas (VerfasserIn), Ravens, Ursula (VerfasserIn), Bieback, Karen (VerfasserIn), Borggrefe, Martin (VerfasserIn), Zhou, Xiao-Bo (VerfasserIn), Akın, Ibrahim (VerfasserIn)
Titel: Serum of patients with acute myocardial infarction prevents inflammation in iPSC-cardiomyocytes/ Katherine Sattler, Ibrahim El-Battrawy, Zhihan Zhao, Christoph Schrottenberg, Gökhan Yücel, Huan Lan, Xin Li, Siegfried Lang, Wolfram-Hubertus Zimmermann, Lukas Cyganek, Jochen Utikal, Thomas Wieland, Ursula Ravens, Karen Bieback, Martin Borggrefe, Xiaobo Zhou & Ibrahim Akin
Format: E-Book-Kapitel
Sprache: Englisch
veröffentlicht:
04 April 2019
Gesamtaufnahme: : Scientific reports, 9(2019) Artikel-Nummer 5651, 13 Seiten
, volume:9
Quelle: Verbunddaten SWB
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Zusammenfassung: Acute myocardial infarction (MI) evokes a systemic inflammatory response and locally the degradation of the necrotic tissue, followed by scar formation. The mechanisms for containment of the infarct zone are not studied well. The study aimed to examine the response of healthy cardiomyocytes to serum of patients with myocardial infarction. Human iPSC-cardiomyocytes (iPSC-CM) generated from two healthy donors were incubated with serum of patients with MI with and without ventricular fibrillation (VF) or of healthy controls. Different cell adhesion molecules were studied by flow cytometry and immunostaining. Cellular electrophysiology was studied by patch clamp. The cell adhesion molecules CD54/ICAM-1, CD58/LFA-3 and CD321/JAM-A were expressed on iPSC-CM within the plasma membrane. Incubation with serum of MI patients reduced the levels of expression of CD54/ICAM-1 and CD321/JAM-A by 15-20%. VF serum was less effective than serum of MI patients without VF. MI serum or VF serum did not affect resting potential, action potential duration or maximum depolarization velocity. Myocardial infarction serum exerts anti-inflammatory effects on healthy cardiomyocytes without affecting their electrical activity, thus helping to contain the infarct zone and to protect healthy tissue. Ventricular fibrillation during MI drives healthy cardiomyocytes towards a pro-inflammatory phenotype.
Beschreibung: Gesehen am 22.05.2019
ISSN: 2045-2322
DOI: 10.1038/s41598-019-42079-z