The cis‐regulatory code of Hox function in Drosophila

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Veröffentlicht in:	The EMBO journal 31(2012), 15, Seite 3323-3333
Personen und Körperschaften:	Sorge, Sebastian (VerfasserIn), Ha, Nati (VerfasserIn), Polychronidou, Maria (VerfasserIn), Friedrich, Jana (VerfasserIn), Kaspar, Petra (VerfasserIn), Mundorf, Juliane (VerfasserIn), Rätzer, Jenny (VerfasserIn), Papagiannouli, Fani (VerfasserIn), Lohmann, Ingrid (VerfasserIn)
Titel:	The cis‐regulatory code of Hox function in Drosophila/ Sebastian Sorge, Nati Ha, Maria Polychronidou, Jana Friedrich, Daniela Bezdan, Petra Kaspar, Martin H. Schaefer, Stephan Ossowski, Stefan R. Henz, Juliane Mundorf, Jenny Rätzer, Fani Papagiannouli and Ingrid Lohmann
Format:	E-Book-Kapitel
Sprache:	Englisch
veröffentlicht:	10 July 2012
Gesamtaufnahme:	European Molecular Biology Organization: The EMBO journal, 31(2012), 15, Seite 3323-3333 , volume:31
Schlagwörter:	ChIP‐seq Deformed Drosophila Hox transcriptional gene regulation
Quelle:	Verbunddaten SWB Lizenzfreie Online-Ressourcen
Errata:	Sorge, Sebastian: Corrigendum

Details
Zusammenfassung:	Precise gene expression is a fundamental aspect of organismal function and depends on the combinatorial interplay of transcription factors (TFs) with cis‐regulatory DNA elements. While much is known about TF function in general, our understanding of their cell type‐specific activities is still poor. To address how widely expressed transcriptional regulators modulate downstream gene activity with high cellular specificity, we have identified binding regions for the Hox TF Deformed (Dfd) in the Drosophila genome. Our analysis of architectural features within Hox cis‐regulatory response elements (HREs) shows that HRE structure is essential for cell type‐specific gene expression. We also find that Dfd and Ultrabithorax (Ubx), another Hox TF specifying different morphological traits, interact with non‐overlapping regions in vivo, despite their similar DNA binding preferences. While Dfd and Ubx HREs exhibit comparable design principles, their motif compositions and motif‐pair associations are distinct, explaining the highly selective interaction of these Hox proteins with the regulatory environment. Thus, our results uncover the regulatory code imprinted in Hox enhancers and elucidate the mechanisms underlying functional specificity of TFs in vivo.
Beschreibung:	Gesehen am 24.08.2017
Umfang:	11
ISSN:	1460-2075
DOI:	10.1038/emboj.2012.179