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Genome-wide analysis reveals a cell cycle-dependent mechanism controlling centromere propagation
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| Veröffentlicht in: | The journal of cell biology 183(2008), 5, Seite 805-818 |
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| Personen und Körperschaften: | |
| Titel: | Genome-wide analysis reveals a cell cycle-dependent mechanism controlling centromere propagation/ Sylvia Erhardt, Barbara G. Mellone, Craig M. Betts, Weiguo Zhang, Gary H. Karpen, and Aaron F. Straight |
| Format: | E-Book-Kapitel |
| Sprache: | Englisch |
| veröffentlicht: |
December 1, 2008
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| Gesamtaufnahme: |
: The journal of cell biology, 183(2008), 5, Seite 805-818
, volume:183 |
| Quelle: | Verbunddaten SWB Lizenzfreie Online-Ressourcen |
| Zusammenfassung: | Centromeres are the structural and functional foundation for kinetochore formation, spindle attachment, and chromosome segregation. In this study, we isolated factors required for centromere propagation using genome-wide RNA interference screening for defects in centromere protein A (CENP-A; centromere identifier [CID]) localization in Drosophila melanogaster. We identified the proteins CAL1 and CENP-C as essential factors for CID assembly at the centromere. CID, CAL1, and CENP-C coimmunoprecipitate and are mutually dependent for centromere localization and function. We also identified the mitotic cyclin A (CYCA) and the anaphase-promoting complex (APC) inhibitor RCA1/Emi1 as regulators of centromere propagation. We show that CYCA is centromere localized and that CYCA and RCA1/Emi1 couple centromere assembly to the cell cycle through regulation of the fizzy-related/CDH1 subunit of the APC. Our findings identify essential components of the epigenetic machinery that ensures proper specification and propagation of the centromere and suggest a mechanism for coordinating centromere inheritance with cell division. |
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| Beschreibung: | Gesehen am 15.08.2017 |
| Umfang: | 14 |
| ISSN: |
1540-8140
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| DOI: | 10.1083/jcb.200806038 |