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In vitro centromere and kinetochore assembly on defined chromatin templates
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| Veröffentlicht in: | Nature <London> 477(2011), 7364, Seite 354-358 |
|---|---|
| Personen und Körperschaften: | |
| Titel: | In vitro centromere and kinetochore assembly on defined chromatin templates/ Annika Guse, Christopher W. Carroll, Ben Moree, Colin J. Fuller, Aaron F. Straight |
| Format: | E-Book-Kapitel |
| Sprache: | Englisch |
| veröffentlicht: |
18 August 2011
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| Gesamtaufnahme: |
: Nature <London>, 477(2011), 7364, Seite 354-358
, volume:477 |
| Schlagwörter: | |
| Quelle: | Verbunddaten SWB Lizenzfreie Online-Ressourcen |
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| 520 | |a During cell division, chromosomes are segregated to nascent daughter cells by attaching to the microtubules of the mitotic spindle through the kinetochore. Kinetochores are assembled on a specialized chromatin domain called the centromere, which is characterized by the replacement of nucleosomal histone H3 with the histone H3 variant centromere protein A (CENP-A). CENP-A is essential for centromere and kinetochore formation in all eukaryotes but it is unknown how CENP-A chromatin directs centromere and kinetochore assembly. Here we generate synthetic CENP-A chromatin that recapitulates essential steps of centromere and kinetochore assembly in vitro. We show that reconstituted CENP-A chromatin when added to cell-free extracts is sufficient for the assembly of centromere and kinetochore proteins, microtubule binding and stabilization, and mitotic checkpoint function. Using chromatin assembled from histone H3/CENP-A chimaeras, we demonstrate that the conserved carboxy terminus of CENP-A is necessary and sufficient for centromere and kinetochore protein recruitment and function but that the CENP-A targeting domain—required for new CENP-A histone assembly—is not. These data show that two of the primary requirements for accurate chromosome segregation, the assembly of the kinetochore and the propagation of CENP-A chromatin, are specified by different elements in the CENP-A histone. Our unique cell-free system enables complete control and manipulation of the chromatin substrate and thus presents a powerful tool to study centromere and kinetochore assembly. | ||
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| contents | During cell division, chromosomes are segregated to nascent daughter cells by attaching to the microtubules of the mitotic spindle through the kinetochore. Kinetochores are assembled on a specialized chromatin domain called the centromere, which is characterized by the replacement of nucleosomal histone H3 with the histone H3 variant centromere protein A (CENP-A). CENP-A is essential for centromere and kinetochore formation in all eukaryotes but it is unknown how CENP-A chromatin directs centromere and kinetochore assembly. Here we generate synthetic CENP-A chromatin that recapitulates essential steps of centromere and kinetochore assembly in vitro. We show that reconstituted CENP-A chromatin when added to cell-free extracts is sufficient for the assembly of centromere and kinetochore proteins, microtubule binding and stabilization, and mitotic checkpoint function. Using chromatin assembled from histone H3/CENP-A chimaeras, we demonstrate that the conserved carboxy terminus of CENP-A is necessary and sufficient for centromere and kinetochore protein recruitment and function but that the CENP-A targeting domain—required for new CENP-A histone assembly—is not. These data show that two of the primary requirements for accurate chromosome segregation, the assembly of the kinetochore and the propagation of CENP-A chromatin, are specified by different elements in the CENP-A histone. Our unique cell-free system enables complete control and manipulation of the chromatin substrate and thus presents a powerful tool to study centromere and kinetochore assembly. |
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| spelling | Guse, Annika VerfasserIn (DE-588)1079253254 (DE-627)840692730 (DE-576)452309247 aut, In vitro centromere and kinetochore assembly on defined chromatin templates Annika Guse, Christopher W. Carroll, Ben Moree, Colin J. Fuller, Aaron F. Straight, 18 August 2011, 5, Text txt rdacontent, Computermedien c rdamedia, Online-Ressource cr rdacarrier, Gesehen am 10.05.2017, During cell division, chromosomes are segregated to nascent daughter cells by attaching to the microtubules of the mitotic spindle through the kinetochore. Kinetochores are assembled on a specialized chromatin domain called the centromere, which is characterized by the replacement of nucleosomal histone H3 with the histone H3 variant centromere protein A (CENP-A). CENP-A is essential for centromere and kinetochore formation in all eukaryotes but it is unknown how CENP-A chromatin directs centromere and kinetochore assembly. Here we generate synthetic CENP-A chromatin that recapitulates essential steps of centromere and kinetochore assembly in vitro. We show that reconstituted CENP-A chromatin when added to cell-free extracts is sufficient for the assembly of centromere and kinetochore proteins, microtubule binding and stabilization, and mitotic checkpoint function. Using chromatin assembled from histone H3/CENP-A chimaeras, we demonstrate that the conserved carboxy terminus of CENP-A is necessary and sufficient for centromere and kinetochore protein recruitment and function but that the CENP-A targeting domain—required for new CENP-A histone assembly—is not. These data show that two of the primary requirements for accurate chromosome segregation, the assembly of the kinetochore and the propagation of CENP-A chromatin, are specified by different elements in the CENP-A histone. Our unique cell-free system enables complete control and manipulation of the chromatin substrate and thus presents a powerful tool to study centromere and kinetochore assembly., Biochemistry, Cell biology, Molecular biology, Enthalten in Nature <London> London [u.a.] : Nature Publ. Group, 1869 477(2011), 7364, Seite 354-358 Online-Ressource (DE-627)240151402 (DE-600)1413423-8 (DE-576)079718426 1476-4687 nnns, volume:477 year:2011 number:7364 pages:354-358 extent:5, http://dx.doi.org/10.1038/nature10379 Verlag Resolving-System kostenfrei Volltext, https://www.nature.com/nature/journal/v477/n7364/full/nature10379.html Verlag kostenfrei Volltext, http://dx.doi.org/10.1038/nature10379 LFER, LFER 2017-06-08T00:00:00Z |
| spellingShingle | Guse, Annika, In vitro centromere and kinetochore assembly on defined chromatin templates, During cell division, chromosomes are segregated to nascent daughter cells by attaching to the microtubules of the mitotic spindle through the kinetochore. Kinetochores are assembled on a specialized chromatin domain called the centromere, which is characterized by the replacement of nucleosomal histone H3 with the histone H3 variant centromere protein A (CENP-A). CENP-A is essential for centromere and kinetochore formation in all eukaryotes but it is unknown how CENP-A chromatin directs centromere and kinetochore assembly. Here we generate synthetic CENP-A chromatin that recapitulates essential steps of centromere and kinetochore assembly in vitro. We show that reconstituted CENP-A chromatin when added to cell-free extracts is sufficient for the assembly of centromere and kinetochore proteins, microtubule binding and stabilization, and mitotic checkpoint function. Using chromatin assembled from histone H3/CENP-A chimaeras, we demonstrate that the conserved carboxy terminus of CENP-A is necessary and sufficient for centromere and kinetochore protein recruitment and function but that the CENP-A targeting domain—required for new CENP-A histone assembly—is not. These data show that two of the primary requirements for accurate chromosome segregation, the assembly of the kinetochore and the propagation of CENP-A chromatin, are specified by different elements in the CENP-A histone. Our unique cell-free system enables complete control and manipulation of the chromatin substrate and thus presents a powerful tool to study centromere and kinetochore assembly., Biochemistry, Cell biology, Molecular biology |
| swb_id_str | 488393485 |
| title | In vitro centromere and kinetochore assembly on defined chromatin templates |
| title_auth | In vitro centromere and kinetochore assembly on defined chromatin templates |
| title_full | In vitro centromere and kinetochore assembly on defined chromatin templates Annika Guse, Christopher W. Carroll, Ben Moree, Colin J. Fuller, Aaron F. Straight |
| title_fullStr | In vitro centromere and kinetochore assembly on defined chromatin templates Annika Guse, Christopher W. Carroll, Ben Moree, Colin J. Fuller, Aaron F. Straight |
| title_full_unstemmed | In vitro centromere and kinetochore assembly on defined chromatin templates Annika Guse, Christopher W. Carroll, Ben Moree, Colin J. Fuller, Aaron F. Straight |
| title_in_hierarchy | In vitro centromere and kinetochore assembly on defined chromatin templates / Annika Guse, Christopher W. Carroll, Ben Moree, Colin J. Fuller, Aaron F. Straight, |
| title_short | In vitro centromere and kinetochore assembly on defined chromatin templates |
| title_sort | in vitro centromere and kinetochore assembly on defined chromatin templates |
| topic | Biochemistry, Cell biology, Molecular biology |
| topic_facet | Biochemistry, Cell biology, Molecular biology |
| url | http://dx.doi.org/10.1038/nature10379, https://www.nature.com/nature/journal/v477/n7364/full/nature10379.html |