Eintrag weiter verarbeiten
In vitro centromere and kinetochore assembly on defined chromatin templates
Gespeichert in:
| Veröffentlicht in: | Nature <London> 477(2011), 7364, Seite 354-358 |
|---|---|
| Personen und Körperschaften: | |
| Titel: | In vitro centromere and kinetochore assembly on defined chromatin templates/ Annika Guse, Christopher W. Carroll, Ben Moree, Colin J. Fuller, Aaron F. Straight |
| Format: | E-Book-Kapitel |
| Sprache: | Englisch |
| veröffentlicht: |
18 August 2011
|
| Gesamtaufnahme: |
: Nature <London>, 477(2011), 7364, Seite 354-358
, volume:477 |
| Schlagwörter: | |
| Quelle: | Verbunddaten SWB Lizenzfreie Online-Ressourcen |
| Zusammenfassung: | During cell division, chromosomes are segregated to nascent daughter cells by attaching to the microtubules of the mitotic spindle through the kinetochore. Kinetochores are assembled on a specialized chromatin domain called the centromere, which is characterized by the replacement of nucleosomal histone H3 with the histone H3 variant centromere protein A (CENP-A). CENP-A is essential for centromere and kinetochore formation in all eukaryotes but it is unknown how CENP-A chromatin directs centromere and kinetochore assembly. Here we generate synthetic CENP-A chromatin that recapitulates essential steps of centromere and kinetochore assembly in vitro. We show that reconstituted CENP-A chromatin when added to cell-free extracts is sufficient for the assembly of centromere and kinetochore proteins, microtubule binding and stabilization, and mitotic checkpoint function. Using chromatin assembled from histone H3/CENP-A chimaeras, we demonstrate that the conserved carboxy terminus of CENP-A is necessary and sufficient for centromere and kinetochore protein recruitment and function but that the CENP-A targeting domain—required for new CENP-A histone assembly—is not. These data show that two of the primary requirements for accurate chromosome segregation, the assembly of the kinetochore and the propagation of CENP-A chromatin, are specified by different elements in the CENP-A histone. Our unique cell-free system enables complete control and manipulation of the chromatin substrate and thus presents a powerful tool to study centromere and kinetochore assembly. |
|---|---|
| Beschreibung: | Gesehen am 10.05.2017 |
| Umfang: | 5 |
| ISSN: |
1476-4687
|
| DOI: | 10.1038/nature10379 |