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Biodegradable poly (lactic acid-co-glycolic acid) scaffolds as carriers for geneticallymodified fibroblasts

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Veröffentlicht in: PLOS ONE Vol. 12.2017, 4, art. no. e0174860, insgesamt 16 Seiten
Personen und Körperschaften: Perisic, Tatjana (VerfasserIn), Zhang, Ziyang (VerfasserIn), Foehr, Peter (VerfasserIn), Höpfner, Ursula (VerfasserIn), Klutz, Kathrin (VerfasserIn), Burgkart, Rainer (VerfasserIn), Slobodianski, Alexei (VerfasserIn), Göldner, Moritz (VerfasserIn), Machens, Hans-Günther (VerfasserIn), Schilling, Arndt (VerfasserIn), Technische Universität Hamburg (Sonstige, Sonstige Körperschaft), Technische Universität Hamburg Institut für Technologie- und Innovationsmanagement (Sonstige, Sonstige Körperschaft)
Titel: Biodegradable poly (lactic acid-co-glycolic acid) scaffolds as carriers for geneticallymodified fibroblasts/ Tatjana Perisic, Ziyang Zhang, Peter Foehr, Ursula Hopfner, Kathrin Klutz; Rainer H. Burgkart, Alexei Slobodianski, Moritz Goeldner; Hans-Günther Machens, Arndt F. Schilling
Format: E-Book-Kapitel
Sprache: Englisch
veröffentlicht:
April 5, 2017
Gesamtaufnahme: : PLOS ONE, Vol. 12.2017, 4, art. no. e0174860, insgesamt 16 Seiten
, volume:12
Quelle: Verbunddaten SWB
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Zusammenfassung: Recent advances in gene delivery into cells allow improved therapeutic effects in gene therapy trials. To increase the bioavailability of applied cells, it is of great interest that transfected cells remain at the application site and systemic spread is minimized. In this study, we tested clinically used biodegradable poly(lactic acid-co-glycolic acid) (PLGA) scaffolds (Vicryl & Ethisorb) as transient carriers for genetically modified cells. To this aim, we used human fibroblasts and examined attachment and proliferation of untransfected cells on the scaffolds in vitro, as well as the mechanical properties of the scaffolds at four time points (1, 3, 6 and 9 days) of cultivation. Furthermore, the adherence of cells transfected with green fluorescent protein (GFP) and vascular endothelial growth factor (VEGF165) and also VEGF165 protein secretion were investigated. Our results show that human fibroblasts adhere on both types of PLGA scaffolds. However, proliferation and transgene expression capacity were higher on Ethisorb scaffolds most probably due to a different architecture of the scaffold. Additionally, cultivation of the cells on the scaffolds did not alter their biomechanical properties. The results of this investigation could be potentially exploited in therapeutic regiments with areal delivery of transiently transfected cells and may open the way for a variety of applications of cell-based gene therapy, tissue engineering and regenerative medicine.
Umfang: Illustrationen, Diagramme
ISSN: 1932-6203
DOI: 10.15480/882.1993