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Zusammenfassung: <jats:p> Dissimilatory reduction of labeled nitrate in the presence of nonlabeled N<jats:sub>2</jats:sub>O, or NO, was studied in forest mor humus during anaerobic incubation. Data on the isotopic composition of the N<jats:sub>2</jats:sub>O and N<jats:sub>2</jats:sub> pools suggested that all of the N<jats:sub>2</jats:sub> formed had passed the N<jats:sub>2</jats:sub>O pool. This finding supported the earlier suggestion that N<jats:sub>2</jats:sub>O is an obligatory intermediate in the dissimilatory reduction of nitrate to N<jats:sub>2</jats:sub>. Due to a likely occurrence of nonenzymatic isotopic exchange between NO and nitrate, the technique used was considered unreliable to study the nature of NO as a possible intermediate in the sequence of nitrate reduction. However, qualitative observations indicated that NO was not a free, obligatory intermediate. Reduction of nitrate was strongly inhibited by the presence of NO. Reduction of N<jats:sub>2</jats:sub>O to N<jats:sub>2</jats:sub> was significantly retarded by the presence of nitrate. The length of the lag period increased as the initial nitrate concentration was increased. Key words: Dissimilatory nitrate reduction, denitrification, nitrous oxide, nitric oxide </jats:p>
Umfang: 21-29
ISSN: 0008-4271
1918-1841
DOI: 10.4141/cjss84-003