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Identification of timothy cultivars by SDS-PAGE analysis of seed storage proteins
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| Zeitschriftentitel: | Canadian Journal of Plant Science |
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| Personen und Körperschaften: | , |
| In: | Canadian Journal of Plant Science, 72, 1992, 4, S. 1215-1222 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Canadian Science Publishing
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| Schlagwörter: |
| Zusammenfassung: | <jats:p> SDS-PAGE analysis of seed proteins was carried out to identify the cultivars in the forage crop, timothy (Phleum pratense L.). Nineteen cultivars of timothy were examined. Among them five were from Europe and fourteen from North America. In total fifty protein bands were detected in mature seed extract by SDS-PAGE followed by Coomassie blue staining. Except for two pairs, all the cultivars were differentiated by SDS-PAGE analysis of seed storage proteins. In the electrophoretic profile, no protein bands were found to be specific either to European or to North American cultivars which is an indication of their genetic similarity. Twelve samples of cultivar Toro harvested from Alberta and Manitoba (Canada), Idaho and Minnesota (USA) were compared and no significant differences were found in their seed protein banding patterns, which suggests environmental stability of timothy seed proteins.Key words: SDS PAGE, timothy cultivar identification, seed storage proteins </jats:p> |
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| Umfang: | 1215-1222 |
| ISSN: |
0008-4220
1918-1833 |
| DOI: | 10.4141/cjps92-148 |