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Generation of Feeder-Free Pig Induced Pluripotent Stem Cells without Pou5f1
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| Zeitschriftentitel: | Cell Transplantation |
|---|---|
| Personen und Körperschaften: | , , , , , , , , |
| In: | Cell Transplantation, 21, 2012, 5, S. 815-825 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
SAGE Publications
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| Schlagwörter: |
| author_facet |
Montserrat, Nuria De Oñate, Lorena Garreta, Elena Gonzãlez, Federico Adamo, Antonio Eguizãbal, Cristina Häfner, Sophia Vassena, Rita Belmonte, Juan Carlos Izpisua Montserrat, Nuria De Oñate, Lorena Garreta, Elena Gonzãlez, Federico Adamo, Antonio Eguizãbal, Cristina Häfner, Sophia Vassena, Rita Belmonte, Juan Carlos Izpisua |
|---|---|
| author |
Montserrat, Nuria De Oñate, Lorena Garreta, Elena Gonzãlez, Federico Adamo, Antonio Eguizãbal, Cristina Häfner, Sophia Vassena, Rita Belmonte, Juan Carlos Izpisua |
| spellingShingle |
Montserrat, Nuria De Oñate, Lorena Garreta, Elena Gonzãlez, Federico Adamo, Antonio Eguizãbal, Cristina Häfner, Sophia Vassena, Rita Belmonte, Juan Carlos Izpisua Cell Transplantation Generation of Feeder-Free Pig Induced Pluripotent Stem Cells without Pou5f1 Transplantation Cell Biology Biomedical Engineering |
| author_sort |
montserrat, nuria |
| spelling |
Montserrat, Nuria De Oñate, Lorena Garreta, Elena Gonzãlez, Federico Adamo, Antonio Eguizãbal, Cristina Häfner, Sophia Vassena, Rita Belmonte, Juan Carlos Izpisua 0963-6897 1555-3892 SAGE Publications Transplantation Cell Biology Biomedical Engineering http://dx.doi.org/10.3727/096368911x601019 <jats:p> The pig represents an ideal large-animal model, intermediate between rodents and humans, for the preclinical assessment of emerging cell therapies. As no validated pig embryonic stem (pES) cell lines have been derived so far, pig induced pluripotent stem cells (piPSCs) should offer an alternative source of undifferentiated cells to advance regenerative medicine research from bench to clinical trial. We report here for the first time the derivation of piPSCs from adult fibroblast with only three transcription factors: Sox2 (sex determining region Y-box 2), Klf4 (Krüppel-like factor 4), and c-Myc (avian myelocytomatosis viral oncogene homolog). We have been able to demonstrate that exogenous Pou5f1 (POU domain class 5 transcription factor 1; abbreviated as Octamer-4: Oct4) is dispensable to achieve and maintain pluripotency in the generation of piPSCs. To the best of our knowledge, this is also the first report of somatic reprogramming in any species without the overexpression, either directly or indirectly, of Oct4. Moreover, we were able to generate piPSCs without the use of feeder cells, approaching thus xeno-free conditions. Our work paves the way for the derivation of clinical grade piPSCs for regenerative medicine. </jats:p> Generation of Feeder-Free Pig Induced Pluripotent Stem Cells without Pou5f1 Cell Transplantation |
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| title |
Generation of Feeder-Free Pig Induced Pluripotent Stem Cells without Pou5f1 |
| title_unstemmed |
Generation of Feeder-Free Pig Induced Pluripotent Stem Cells without Pou5f1 |
| title_full |
Generation of Feeder-Free Pig Induced Pluripotent Stem Cells without Pou5f1 |
| title_fullStr |
Generation of Feeder-Free Pig Induced Pluripotent Stem Cells without Pou5f1 |
| title_full_unstemmed |
Generation of Feeder-Free Pig Induced Pluripotent Stem Cells without Pou5f1 |
| title_short |
Generation of Feeder-Free Pig Induced Pluripotent Stem Cells without Pou5f1 |
| title_sort |
generation of feeder-free pig induced pluripotent stem cells without pou5f1 |
| topic |
Transplantation Cell Biology Biomedical Engineering |
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http://dx.doi.org/10.3727/096368911x601019 |
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2012 |
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815-825 |
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<jats:p> The pig represents an ideal large-animal model, intermediate between rodents and humans, for the preclinical assessment of emerging cell therapies. As no validated pig embryonic stem (pES) cell lines have been derived so far, pig induced pluripotent stem cells (piPSCs) should offer an alternative source of undifferentiated cells to advance regenerative medicine research from bench to clinical trial. We report here for the first time the derivation of piPSCs from adult fibroblast with only three transcription factors: Sox2 (sex determining region Y-box 2), Klf4 (Krüppel-like factor 4), and c-Myc (avian myelocytomatosis viral oncogene homolog). We have been able to demonstrate that exogenous Pou5f1 (POU domain class 5 transcription factor 1; abbreviated as Octamer-4: Oct4) is dispensable to achieve and maintain pluripotency in the generation of piPSCs. To the best of our knowledge, this is also the first report of somatic reprogramming in any species without the overexpression, either directly or indirectly, of Oct4. Moreover, we were able to generate piPSCs without the use of feeder cells, approaching thus xeno-free conditions. Our work paves the way for the derivation of clinical grade piPSCs for regenerative medicine. </jats:p> |
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| author | Montserrat, Nuria, De Oñate, Lorena, Garreta, Elena, Gonzãlez, Federico, Adamo, Antonio, Eguizãbal, Cristina, Häfner, Sophia, Vassena, Rita, Belmonte, Juan Carlos Izpisua |
| author_facet | Montserrat, Nuria, De Oñate, Lorena, Garreta, Elena, Gonzãlez, Federico, Adamo, Antonio, Eguizãbal, Cristina, Häfner, Sophia, Vassena, Rita, Belmonte, Juan Carlos Izpisua, Montserrat, Nuria, De Oñate, Lorena, Garreta, Elena, Gonzãlez, Federico, Adamo, Antonio, Eguizãbal, Cristina, Häfner, Sophia, Vassena, Rita, Belmonte, Juan Carlos Izpisua |
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| description | <jats:p> The pig represents an ideal large-animal model, intermediate between rodents and humans, for the preclinical assessment of emerging cell therapies. As no validated pig embryonic stem (pES) cell lines have been derived so far, pig induced pluripotent stem cells (piPSCs) should offer an alternative source of undifferentiated cells to advance regenerative medicine research from bench to clinical trial. We report here for the first time the derivation of piPSCs from adult fibroblast with only three transcription factors: Sox2 (sex determining region Y-box 2), Klf4 (Krüppel-like factor 4), and c-Myc (avian myelocytomatosis viral oncogene homolog). We have been able to demonstrate that exogenous Pou5f1 (POU domain class 5 transcription factor 1; abbreviated as Octamer-4: Oct4) is dispensable to achieve and maintain pluripotency in the generation of piPSCs. To the best of our knowledge, this is also the first report of somatic reprogramming in any species without the overexpression, either directly or indirectly, of Oct4. Moreover, we were able to generate piPSCs without the use of feeder cells, approaching thus xeno-free conditions. Our work paves the way for the derivation of clinical grade piPSCs for regenerative medicine. </jats:p> |
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| spelling | Montserrat, Nuria De Oñate, Lorena Garreta, Elena Gonzãlez, Federico Adamo, Antonio Eguizãbal, Cristina Häfner, Sophia Vassena, Rita Belmonte, Juan Carlos Izpisua 0963-6897 1555-3892 SAGE Publications Transplantation Cell Biology Biomedical Engineering http://dx.doi.org/10.3727/096368911x601019 <jats:p> The pig represents an ideal large-animal model, intermediate between rodents and humans, for the preclinical assessment of emerging cell therapies. As no validated pig embryonic stem (pES) cell lines have been derived so far, pig induced pluripotent stem cells (piPSCs) should offer an alternative source of undifferentiated cells to advance regenerative medicine research from bench to clinical trial. We report here for the first time the derivation of piPSCs from adult fibroblast with only three transcription factors: Sox2 (sex determining region Y-box 2), Klf4 (Krüppel-like factor 4), and c-Myc (avian myelocytomatosis viral oncogene homolog). We have been able to demonstrate that exogenous Pou5f1 (POU domain class 5 transcription factor 1; abbreviated as Octamer-4: Oct4) is dispensable to achieve and maintain pluripotency in the generation of piPSCs. To the best of our knowledge, this is also the first report of somatic reprogramming in any species without the overexpression, either directly or indirectly, of Oct4. Moreover, we were able to generate piPSCs without the use of feeder cells, approaching thus xeno-free conditions. Our work paves the way for the derivation of clinical grade piPSCs for regenerative medicine. </jats:p> Generation of Feeder-Free Pig Induced Pluripotent Stem Cells without Pou5f1 Cell Transplantation |
| spellingShingle | Montserrat, Nuria, De Oñate, Lorena, Garreta, Elena, Gonzãlez, Federico, Adamo, Antonio, Eguizãbal, Cristina, Häfner, Sophia, Vassena, Rita, Belmonte, Juan Carlos Izpisua, Cell Transplantation, Generation of Feeder-Free Pig Induced Pluripotent Stem Cells without Pou5f1, Transplantation, Cell Biology, Biomedical Engineering |
| title | Generation of Feeder-Free Pig Induced Pluripotent Stem Cells without Pou5f1 |
| title_full | Generation of Feeder-Free Pig Induced Pluripotent Stem Cells without Pou5f1 |
| title_fullStr | Generation of Feeder-Free Pig Induced Pluripotent Stem Cells without Pou5f1 |
| title_full_unstemmed | Generation of Feeder-Free Pig Induced Pluripotent Stem Cells without Pou5f1 |
| title_short | Generation of Feeder-Free Pig Induced Pluripotent Stem Cells without Pou5f1 |
| title_sort | generation of feeder-free pig induced pluripotent stem cells without pou5f1 |
| title_unstemmed | Generation of Feeder-Free Pig Induced Pluripotent Stem Cells without Pou5f1 |
| topic | Transplantation, Cell Biology, Biomedical Engineering |
| url | http://dx.doi.org/10.3727/096368911x601019 |