Electrochemical Enzyme Biosensor Bearing Biochar Nanoparticle as Signal Enhancer for Bisphenol A Detection in Water

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Bibliographische Detailangaben
Zeitschriftentitel:	Sensors
Personen und Körperschaften:	Liu, Yang, Yao, Lan, He, Lingzhi, Liu, Na, Piao, Yunxian
In:	Sensors, 19, 2019, 7, S. 1619
Format:	E-Article
Sprache:	Englisch
veröffentlicht:	MDPI AG
Schlagwörter:	Electrical and Electronic Engineering Biochemistry Instrumentation Atomic and Molecular Physics, and Optics Analytical Chemistry

Details
Zusammenfassung:	<jats:p>An electrochemical tyrosinase enzyme (Tyr) biosensor using a highly conductive sugarcane derived biochar nanoparticle (BCNP) as a transducer and signal enhancer (BCNPs/Tyr/Nafion/GCE) was developed for the sensitive detection of bisphenol A (BPA). The BCNPs/Tyr/Nafion/GCE biosensor exhibited improved amperometric current responses such as higher sensing signal, decreased impedance and lowered reduction potential compared with the Tyr/Nafion/GCE due to high conductivity property of the biochar nanoparticle. Under the optimized conditions, it could detect BPA in good sensitivity with linear range from 0.02 to 10 μM, and a lowest detection limit of 3.18 nM. Moreover, it showed a low Km value, high reproducibility and good selectivity over other reagents, and the BCNPs/Tyr complex solution also showed good stability with 86.9% of sensing signal maintained after one month storage. The biosensor was also successfully utilized for real water detection with high accuracy as validated by high performance liquid chromatography. Therefore, the biochar nanoparticle based enzyme biosensor proved to be a potential and reliable method for high performance detection of pollutants in the environment.</jats:p>
Umfang:	1619
ISSN:	1424-8220
DOI:	10.3390/s19071619