Eintrag weiter verarbeiten
Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It’s Protective Effects against Parkinson’s Disease Mouse Model
Gespeichert in:
| Zeitschriftentitel: | Molecules |
|---|---|
| Personen und Körperschaften: | , , , , , , , |
| In: | Molecules, 24, 2019, 7, S. 1435 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
MDPI AG
|
| Schlagwörter: |
| author_facet |
Lee, Hee Ju Dhodary, Basanta Lee, Ju Yong An, Jin-Pyo Ryu, Young-Kyoung Kim, Kyoung-Shim Lee, Chul-Ho Oh, Won Keun Lee, Hee Ju Dhodary, Basanta Lee, Ju Yong An, Jin-Pyo Ryu, Young-Kyoung Kim, Kyoung-Shim Lee, Chul-Ho Oh, Won Keun |
|---|---|
| author |
Lee, Hee Ju Dhodary, Basanta Lee, Ju Yong An, Jin-Pyo Ryu, Young-Kyoung Kim, Kyoung-Shim Lee, Chul-Ho Oh, Won Keun |
| spellingShingle |
Lee, Hee Ju Dhodary, Basanta Lee, Ju Yong An, Jin-Pyo Ryu, Young-Kyoung Kim, Kyoung-Shim Lee, Chul-Ho Oh, Won Keun Molecules Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It’s Protective Effects against Parkinson’s Disease Mouse Model Chemistry (miscellaneous) Analytical Chemistry Organic Chemistry Physical and Theoretical Chemistry Molecular Medicine Drug Discovery Pharmaceutical Science |
| author_sort |
lee, hee ju |
| spelling |
Lee, Hee Ju Dhodary, Basanta Lee, Ju Yong An, Jin-Pyo Ryu, Young-Kyoung Kim, Kyoung-Shim Lee, Chul-Ho Oh, Won Keun 1420-3049 MDPI AG Chemistry (miscellaneous) Analytical Chemistry Organic Chemistry Physical and Theoretical Chemistry Molecular Medicine Drug Discovery Pharmaceutical Science http://dx.doi.org/10.3390/molecules24071435 <jats:p>Humulus japonicus is an annual plant belonging to the Cannabacea family, and it has been traditionally used to treat pulmonary tuberculosis, dysentery, chronic colitis, and hypertension. We investigated the active components against Parkinson’s disease from H. japonicus fraction (HJF) using high performance liquid chromatography (HPLC) coupled with quadruple-time-of-flight mass spectroscopy (qTOF-MS) and NMR. Fourteen compounds were isolated from HJF, including one new compound, using HPLC-qTOF-MS and NMR. The major compounds of HJF were luteolin-7-O-glucoside and apigenin-7-O-glucoside, and there was approximately 12.57- and 9.68-folds increase in the contents of these flavonoids compared to those of the 70% EtOH extract. Apigenin and luteolin exhibited the strongest inhibitory effects on monoamine oxidase (MAO) B enzyme activity. In animal studies, limb-use behavior was significantly reduced by unilateral 6-OHDA lesion and ipsilateral rotations. These results indicated that oral administration of 300 mg/kg HJF resulted in the improvement of motor asymmetry and motor impairment in unilateral 6-OHDA-lesioned mice. HJF, including active components leads to an improvement of motor behavior in a Parkinson’s disease mouse model.</jats:p> Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It’s Protective Effects against Parkinson’s Disease Mouse Model Molecules |
| doi_str_mv |
10.3390/molecules24071435 |
| facet_avail |
Online Free |
| finc_class_facet |
Chemie und Pharmazie Technik Physik Medizin |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMzM5MC9tb2xlY3VsZXMyNDA3MTQzNQ |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMzM5MC9tb2xlY3VsZXMyNDA3MTQzNQ |
| institution |
DE-Pl11 DE-Rs1 DE-14 DE-105 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Zi4 DE-Gla1 DE-15 |
| imprint |
MDPI AG, 2019 |
| imprint_str_mv |
MDPI AG, 2019 |
| issn |
1420-3049 |
| issn_str_mv |
1420-3049 |
| language |
English |
| mega_collection |
MDPI AG (CrossRef) |
| match_str |
lee2019dereplicationofcomponentscoupledwithhplcqtofmsintheactivefractionofhumulusjaponicusanditsprotectiveeffectsagainstparkinsonsdiseasemousemodel |
| publishDateSort |
2019 |
| publisher |
MDPI AG |
| recordtype |
ai |
| record_format |
ai |
| series |
Molecules |
| source_id |
49 |
| title |
Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It’s Protective Effects against Parkinson’s Disease Mouse Model |
| title_unstemmed |
Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It’s Protective Effects against Parkinson’s Disease Mouse Model |
| title_full |
Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It’s Protective Effects against Parkinson’s Disease Mouse Model |
| title_fullStr |
Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It’s Protective Effects against Parkinson’s Disease Mouse Model |
| title_full_unstemmed |
Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It’s Protective Effects against Parkinson’s Disease Mouse Model |
| title_short |
Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It’s Protective Effects against Parkinson’s Disease Mouse Model |
| title_sort |
dereplication of components coupled with hplc-qtof-ms in the active fraction of humulus japonicus and it’s protective effects against parkinson’s disease mouse model |
| topic |
Chemistry (miscellaneous) Analytical Chemistry Organic Chemistry Physical and Theoretical Chemistry Molecular Medicine Drug Discovery Pharmaceutical Science |
| url |
http://dx.doi.org/10.3390/molecules24071435 |
| publishDate |
2019 |
| physical |
1435 |
| description |
<jats:p>Humulus japonicus is an annual plant belonging to the Cannabacea family, and it has been traditionally used to treat pulmonary tuberculosis, dysentery, chronic colitis, and hypertension. We investigated the active components against Parkinson’s disease from H. japonicus fraction (HJF) using high performance liquid chromatography (HPLC) coupled with quadruple-time-of-flight mass spectroscopy (qTOF-MS) and NMR. Fourteen compounds were isolated from HJF, including one new compound, using HPLC-qTOF-MS and NMR. The major compounds of HJF were luteolin-7-O-glucoside and apigenin-7-O-glucoside, and there was approximately 12.57- and 9.68-folds increase in the contents of these flavonoids compared to those of the 70% EtOH extract. Apigenin and luteolin exhibited the strongest inhibitory effects on monoamine oxidase (MAO) B enzyme activity. In animal studies, limb-use behavior was significantly reduced by unilateral 6-OHDA lesion and ipsilateral rotations. These results indicated that oral administration of 300 mg/kg HJF resulted in the improvement of motor asymmetry and motor impairment in unilateral 6-OHDA-lesioned mice. HJF, including active components leads to an improvement of motor behavior in a Parkinson’s disease mouse model.</jats:p> |
| container_issue |
7 |
| container_start_page |
0 |
| container_title |
Molecules |
| container_volume |
24 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792344230263259136 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T17:04:18.468Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Dereplication+of+Components+Coupled+with+HPLC-qTOF-MS+in+the+Active+Fraction+of+Humulus+japonicus+and+It%E2%80%99s+Protective+Effects+against+Parkinson%E2%80%99s+Disease+Mouse+Model&rft.date=2019-04-11&genre=article&issn=1420-3049&volume=24&issue=7&pages=1435&jtitle=Molecules&atitle=Dereplication+of+Components+Coupled+with+HPLC-qTOF-MS+in+the+Active+Fraction+of+Humulus+japonicus+and+It%E2%80%99s+Protective+Effects+against+Parkinson%E2%80%99s+Disease+Mouse+Model&aulast=Oh&aufirst=Won+Keun&rft_id=info%3Adoi%2F10.3390%2Fmolecules24071435&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792344230263259136 |
| author | Lee, Hee Ju, Dhodary, Basanta, Lee, Ju Yong, An, Jin-Pyo, Ryu, Young-Kyoung, Kim, Kyoung-Shim, Lee, Chul-Ho, Oh, Won Keun |
| author_facet | Lee, Hee Ju, Dhodary, Basanta, Lee, Ju Yong, An, Jin-Pyo, Ryu, Young-Kyoung, Kim, Kyoung-Shim, Lee, Chul-Ho, Oh, Won Keun, Lee, Hee Ju, Dhodary, Basanta, Lee, Ju Yong, An, Jin-Pyo, Ryu, Young-Kyoung, Kim, Kyoung-Shim, Lee, Chul-Ho, Oh, Won Keun |
| author_sort | lee, hee ju |
| container_issue | 7 |
| container_start_page | 0 |
| container_title | Molecules |
| container_volume | 24 |
| description | <jats:p>Humulus japonicus is an annual plant belonging to the Cannabacea family, and it has been traditionally used to treat pulmonary tuberculosis, dysentery, chronic colitis, and hypertension. We investigated the active components against Parkinson’s disease from H. japonicus fraction (HJF) using high performance liquid chromatography (HPLC) coupled with quadruple-time-of-flight mass spectroscopy (qTOF-MS) and NMR. Fourteen compounds were isolated from HJF, including one new compound, using HPLC-qTOF-MS and NMR. The major compounds of HJF were luteolin-7-O-glucoside and apigenin-7-O-glucoside, and there was approximately 12.57- and 9.68-folds increase in the contents of these flavonoids compared to those of the 70% EtOH extract. Apigenin and luteolin exhibited the strongest inhibitory effects on monoamine oxidase (MAO) B enzyme activity. In animal studies, limb-use behavior was significantly reduced by unilateral 6-OHDA lesion and ipsilateral rotations. These results indicated that oral administration of 300 mg/kg HJF resulted in the improvement of motor asymmetry and motor impairment in unilateral 6-OHDA-lesioned mice. HJF, including active components leads to an improvement of motor behavior in a Parkinson’s disease mouse model.</jats:p> |
| doi_str_mv | 10.3390/molecules24071435 |
| facet_avail | Online, Free |
| finc_class_facet | Chemie und Pharmazie, Technik, Physik, Medizin |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMzM5MC9tb2xlY3VsZXMyNDA3MTQzNQ |
| imprint | MDPI AG, 2019 |
| imprint_str_mv | MDPI AG, 2019 |
| institution | DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15 |
| issn | 1420-3049 |
| issn_str_mv | 1420-3049 |
| language | English |
| last_indexed | 2024-03-01T17:04:18.468Z |
| match_str | lee2019dereplicationofcomponentscoupledwithhplcqtofmsintheactivefractionofhumulusjaponicusanditsprotectiveeffectsagainstparkinsonsdiseasemousemodel |
| mega_collection | MDPI AG (CrossRef) |
| physical | 1435 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher | MDPI AG |
| record_format | ai |
| recordtype | ai |
| series | Molecules |
| source_id | 49 |
| spelling | Lee, Hee Ju Dhodary, Basanta Lee, Ju Yong An, Jin-Pyo Ryu, Young-Kyoung Kim, Kyoung-Shim Lee, Chul-Ho Oh, Won Keun 1420-3049 MDPI AG Chemistry (miscellaneous) Analytical Chemistry Organic Chemistry Physical and Theoretical Chemistry Molecular Medicine Drug Discovery Pharmaceutical Science http://dx.doi.org/10.3390/molecules24071435 <jats:p>Humulus japonicus is an annual plant belonging to the Cannabacea family, and it has been traditionally used to treat pulmonary tuberculosis, dysentery, chronic colitis, and hypertension. We investigated the active components against Parkinson’s disease from H. japonicus fraction (HJF) using high performance liquid chromatography (HPLC) coupled with quadruple-time-of-flight mass spectroscopy (qTOF-MS) and NMR. Fourteen compounds were isolated from HJF, including one new compound, using HPLC-qTOF-MS and NMR. The major compounds of HJF were luteolin-7-O-glucoside and apigenin-7-O-glucoside, and there was approximately 12.57- and 9.68-folds increase in the contents of these flavonoids compared to those of the 70% EtOH extract. Apigenin and luteolin exhibited the strongest inhibitory effects on monoamine oxidase (MAO) B enzyme activity. In animal studies, limb-use behavior was significantly reduced by unilateral 6-OHDA lesion and ipsilateral rotations. These results indicated that oral administration of 300 mg/kg HJF resulted in the improvement of motor asymmetry and motor impairment in unilateral 6-OHDA-lesioned mice. HJF, including active components leads to an improvement of motor behavior in a Parkinson’s disease mouse model.</jats:p> Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It’s Protective Effects against Parkinson’s Disease Mouse Model Molecules |
| spellingShingle | Lee, Hee Ju, Dhodary, Basanta, Lee, Ju Yong, An, Jin-Pyo, Ryu, Young-Kyoung, Kim, Kyoung-Shim, Lee, Chul-Ho, Oh, Won Keun, Molecules, Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It’s Protective Effects against Parkinson’s Disease Mouse Model, Chemistry (miscellaneous), Analytical Chemistry, Organic Chemistry, Physical and Theoretical Chemistry, Molecular Medicine, Drug Discovery, Pharmaceutical Science |
| title | Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It’s Protective Effects against Parkinson’s Disease Mouse Model |
| title_full | Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It’s Protective Effects against Parkinson’s Disease Mouse Model |
| title_fullStr | Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It’s Protective Effects against Parkinson’s Disease Mouse Model |
| title_full_unstemmed | Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It’s Protective Effects against Parkinson’s Disease Mouse Model |
| title_short | Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It’s Protective Effects against Parkinson’s Disease Mouse Model |
| title_sort | dereplication of components coupled with hplc-qtof-ms in the active fraction of humulus japonicus and it’s protective effects against parkinson’s disease mouse model |
| title_unstemmed | Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It’s Protective Effects against Parkinson’s Disease Mouse Model |
| topic | Chemistry (miscellaneous), Analytical Chemistry, Organic Chemistry, Physical and Theoretical Chemistry, Molecular Medicine, Drug Discovery, Pharmaceutical Science |
| url | http://dx.doi.org/10.3390/molecules24071435 |