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PAQR3 Modulates Insulin Signaling by Shunting Phosphoinositide 3-Kinase p110α to the Golgi Apparatus
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| Zeitschriftentitel: | Diabetes |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , |
| In: | Diabetes, 62, 2013, 2, S. 444-456 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Diabetes Association
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| Schlagwörter: |
| author_facet |
Wang, Xiao Wang, Lingdi Zhu, Lu Pan, Yi Xiao, Fei Liu, Weizhong Wang, Zhenzhen Guo, Feifan Liu, Yong Thomas, Walter G. Chen, Yan Wang, Xiao Wang, Lingdi Zhu, Lu Pan, Yi Xiao, Fei Liu, Weizhong Wang, Zhenzhen Guo, Feifan Liu, Yong Thomas, Walter G. Chen, Yan |
|---|---|
| author |
Wang, Xiao Wang, Lingdi Zhu, Lu Pan, Yi Xiao, Fei Liu, Weizhong Wang, Zhenzhen Guo, Feifan Liu, Yong Thomas, Walter G. Chen, Yan |
| spellingShingle |
Wang, Xiao Wang, Lingdi Zhu, Lu Pan, Yi Xiao, Fei Liu, Weizhong Wang, Zhenzhen Guo, Feifan Liu, Yong Thomas, Walter G. Chen, Yan Diabetes PAQR3 Modulates Insulin Signaling by Shunting Phosphoinositide 3-Kinase p110α to the Golgi Apparatus Endocrinology, Diabetes and Metabolism Internal Medicine |
| author_sort |
wang, xiao |
| spelling |
Wang, Xiao Wang, Lingdi Zhu, Lu Pan, Yi Xiao, Fei Liu, Weizhong Wang, Zhenzhen Guo, Feifan Liu, Yong Thomas, Walter G. Chen, Yan 0012-1797 1939-327X American Diabetes Association Endocrinology, Diabetes and Metabolism Internal Medicine http://dx.doi.org/10.2337/db12-0244 <jats:p>Phosphoinositide 3-kinase (PI3K) mediates insulin actions by relaying signals from insulin receptors (IRs) to downstream targets. The p110α catalytic subunit of class IA PI3K is the primary insulin-responsive PI3K implicated in insulin signaling. We demonstrate here a new mode of spatial regulation for the p110α subunit of PI3K by PAQR3 that is exclusively localized in the Golgi apparatus. PAQR3 interacts with p110α, and the intracellular targeting of p110α to the Golgi apparatus is reduced by PAQR3 downregulation and increased by PAQR3 overexpression. Insulin-stimulated PI3K activity and phosphoinositide (3,4,5)-triphosphate production are enhanced by Paqr3 deletion and reduced by PAQR3 overexpression in hepatocytes. Deletion of Paqr3 enhances insulin-stimulated phosphorylation of AKT and glycogen synthase kinase 3β, but not phosphorylation of IR and IR substrate-1 (IRS-1), in hepatocytes, mouse liver, and skeletal muscle. Insulin-stimulated GLUT4 translocation to the plasma membrane and glucose uptake are enhanced by Paqr3 ablation. Furthermore, PAQR3 interacts with the domain of p110α involved in its binding with p85, the regulatory subunit of PI3K. Overexpression of PAQR3 dose-dependently reduces the interaction of p85α with p110α. Thus, PAQR3 negatively regulates insulin signaling by shunting cytosolic p110α to the Golgi apparatus while competing with p85 subunit in forming a PI3K complex with p110α.</jats:p> PAQR3 Modulates Insulin Signaling by Shunting Phosphoinositide 3-Kinase p110α to the Golgi Apparatus Diabetes |
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American Diabetes Association, 2013 |
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American Diabetes Association, 2013 |
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0012-1797 1939-327X |
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2013 |
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American Diabetes Association |
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49 |
| title |
PAQR3 Modulates Insulin Signaling by Shunting Phosphoinositide 3-Kinase p110α to the Golgi Apparatus |
| title_unstemmed |
PAQR3 Modulates Insulin Signaling by Shunting Phosphoinositide 3-Kinase p110α to the Golgi Apparatus |
| title_full |
PAQR3 Modulates Insulin Signaling by Shunting Phosphoinositide 3-Kinase p110α to the Golgi Apparatus |
| title_fullStr |
PAQR3 Modulates Insulin Signaling by Shunting Phosphoinositide 3-Kinase p110α to the Golgi Apparatus |
| title_full_unstemmed |
PAQR3 Modulates Insulin Signaling by Shunting Phosphoinositide 3-Kinase p110α to the Golgi Apparatus |
| title_short |
PAQR3 Modulates Insulin Signaling by Shunting Phosphoinositide 3-Kinase p110α to the Golgi Apparatus |
| title_sort |
paqr3 modulates insulin signaling by shunting phosphoinositide 3-kinase p110α to the golgi apparatus |
| topic |
Endocrinology, Diabetes and Metabolism Internal Medicine |
| url |
http://dx.doi.org/10.2337/db12-0244 |
| publishDate |
2013 |
| physical |
444-456 |
| description |
<jats:p>Phosphoinositide 3-kinase (PI3K) mediates insulin actions by relaying signals from insulin receptors (IRs) to downstream targets. The p110α catalytic subunit of class IA PI3K is the primary insulin-responsive PI3K implicated in insulin signaling. We demonstrate here a new mode of spatial regulation for the p110α subunit of PI3K by PAQR3 that is exclusively localized in the Golgi apparatus. PAQR3 interacts with p110α, and the intracellular targeting of p110α to the Golgi apparatus is reduced by PAQR3 downregulation and increased by PAQR3 overexpression. Insulin-stimulated PI3K activity and phosphoinositide (3,4,5)-triphosphate production are enhanced by Paqr3 deletion and reduced by PAQR3 overexpression in hepatocytes. Deletion of Paqr3 enhances insulin-stimulated phosphorylation of AKT and glycogen synthase kinase 3β, but not phosphorylation of IR and IR substrate-1 (IRS-1), in hepatocytes, mouse liver, and skeletal muscle. Insulin-stimulated GLUT4 translocation to the plasma membrane and glucose uptake are enhanced by Paqr3 ablation. Furthermore, PAQR3 interacts with the domain of p110α involved in its binding with p85, the regulatory subunit of PI3K. Overexpression of PAQR3 dose-dependently reduces the interaction of p85α with p110α. Thus, PAQR3 negatively regulates insulin signaling by shunting cytosolic p110α to the Golgi apparatus while competing with p85 subunit in forming a PI3K complex with p110α.</jats:p> |
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| author | Wang, Xiao, Wang, Lingdi, Zhu, Lu, Pan, Yi, Xiao, Fei, Liu, Weizhong, Wang, Zhenzhen, Guo, Feifan, Liu, Yong, Thomas, Walter G., Chen, Yan |
| author_facet | Wang, Xiao, Wang, Lingdi, Zhu, Lu, Pan, Yi, Xiao, Fei, Liu, Weizhong, Wang, Zhenzhen, Guo, Feifan, Liu, Yong, Thomas, Walter G., Chen, Yan, Wang, Xiao, Wang, Lingdi, Zhu, Lu, Pan, Yi, Xiao, Fei, Liu, Weizhong, Wang, Zhenzhen, Guo, Feifan, Liu, Yong, Thomas, Walter G., Chen, Yan |
| author_sort | wang, xiao |
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| container_title | Diabetes |
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| description | <jats:p>Phosphoinositide 3-kinase (PI3K) mediates insulin actions by relaying signals from insulin receptors (IRs) to downstream targets. The p110α catalytic subunit of class IA PI3K is the primary insulin-responsive PI3K implicated in insulin signaling. We demonstrate here a new mode of spatial regulation for the p110α subunit of PI3K by PAQR3 that is exclusively localized in the Golgi apparatus. PAQR3 interacts with p110α, and the intracellular targeting of p110α to the Golgi apparatus is reduced by PAQR3 downregulation and increased by PAQR3 overexpression. Insulin-stimulated PI3K activity and phosphoinositide (3,4,5)-triphosphate production are enhanced by Paqr3 deletion and reduced by PAQR3 overexpression in hepatocytes. Deletion of Paqr3 enhances insulin-stimulated phosphorylation of AKT and glycogen synthase kinase 3β, but not phosphorylation of IR and IR substrate-1 (IRS-1), in hepatocytes, mouse liver, and skeletal muscle. Insulin-stimulated GLUT4 translocation to the plasma membrane and glucose uptake are enhanced by Paqr3 ablation. Furthermore, PAQR3 interacts with the domain of p110α involved in its binding with p85, the regulatory subunit of PI3K. Overexpression of PAQR3 dose-dependently reduces the interaction of p85α with p110α. Thus, PAQR3 negatively regulates insulin signaling by shunting cytosolic p110α to the Golgi apparatus while competing with p85 subunit in forming a PI3K complex with p110α.</jats:p> |
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| imprint | American Diabetes Association, 2013 |
| imprint_str_mv | American Diabetes Association, 2013 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
| issn | 0012-1797, 1939-327X |
| issn_str_mv | 0012-1797, 1939-327X |
| language | English |
| last_indexed | 2024-03-01T16:43:04.681Z |
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| physical | 444-456 |
| publishDate | 2013 |
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| publisher | American Diabetes Association |
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| series | Diabetes |
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| spelling | Wang, Xiao Wang, Lingdi Zhu, Lu Pan, Yi Xiao, Fei Liu, Weizhong Wang, Zhenzhen Guo, Feifan Liu, Yong Thomas, Walter G. Chen, Yan 0012-1797 1939-327X American Diabetes Association Endocrinology, Diabetes and Metabolism Internal Medicine http://dx.doi.org/10.2337/db12-0244 <jats:p>Phosphoinositide 3-kinase (PI3K) mediates insulin actions by relaying signals from insulin receptors (IRs) to downstream targets. The p110α catalytic subunit of class IA PI3K is the primary insulin-responsive PI3K implicated in insulin signaling. We demonstrate here a new mode of spatial regulation for the p110α subunit of PI3K by PAQR3 that is exclusively localized in the Golgi apparatus. PAQR3 interacts with p110α, and the intracellular targeting of p110α to the Golgi apparatus is reduced by PAQR3 downregulation and increased by PAQR3 overexpression. Insulin-stimulated PI3K activity and phosphoinositide (3,4,5)-triphosphate production are enhanced by Paqr3 deletion and reduced by PAQR3 overexpression in hepatocytes. Deletion of Paqr3 enhances insulin-stimulated phosphorylation of AKT and glycogen synthase kinase 3β, but not phosphorylation of IR and IR substrate-1 (IRS-1), in hepatocytes, mouse liver, and skeletal muscle. Insulin-stimulated GLUT4 translocation to the plasma membrane and glucose uptake are enhanced by Paqr3 ablation. Furthermore, PAQR3 interacts with the domain of p110α involved in its binding with p85, the regulatory subunit of PI3K. Overexpression of PAQR3 dose-dependently reduces the interaction of p85α with p110α. Thus, PAQR3 negatively regulates insulin signaling by shunting cytosolic p110α to the Golgi apparatus while competing with p85 subunit in forming a PI3K complex with p110α.</jats:p> PAQR3 Modulates Insulin Signaling by Shunting Phosphoinositide 3-Kinase p110α to the Golgi Apparatus Diabetes |
| spellingShingle | Wang, Xiao, Wang, Lingdi, Zhu, Lu, Pan, Yi, Xiao, Fei, Liu, Weizhong, Wang, Zhenzhen, Guo, Feifan, Liu, Yong, Thomas, Walter G., Chen, Yan, Diabetes, PAQR3 Modulates Insulin Signaling by Shunting Phosphoinositide 3-Kinase p110α to the Golgi Apparatus, Endocrinology, Diabetes and Metabolism, Internal Medicine |
| title | PAQR3 Modulates Insulin Signaling by Shunting Phosphoinositide 3-Kinase p110α to the Golgi Apparatus |
| title_full | PAQR3 Modulates Insulin Signaling by Shunting Phosphoinositide 3-Kinase p110α to the Golgi Apparatus |
| title_fullStr | PAQR3 Modulates Insulin Signaling by Shunting Phosphoinositide 3-Kinase p110α to the Golgi Apparatus |
| title_full_unstemmed | PAQR3 Modulates Insulin Signaling by Shunting Phosphoinositide 3-Kinase p110α to the Golgi Apparatus |
| title_short | PAQR3 Modulates Insulin Signaling by Shunting Phosphoinositide 3-Kinase p110α to the Golgi Apparatus |
| title_sort | paqr3 modulates insulin signaling by shunting phosphoinositide 3-kinase p110α to the golgi apparatus |
| title_unstemmed | PAQR3 Modulates Insulin Signaling by Shunting Phosphoinositide 3-Kinase p110α to the Golgi Apparatus |
| topic | Endocrinology, Diabetes and Metabolism, Internal Medicine |
| url | http://dx.doi.org/10.2337/db12-0244 |