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Upregulated expression of MNX1-AS1 long noncoding RNA predicts poor prognosis in gastric cancer
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| Zeitschriftentitel: | Bosnian Journal of Basic Medical Sciences |
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| Personen und Körperschaften: | , , , , , |
| In: | Bosnian Journal of Basic Medical Sciences, 2019 |
| Format: | E-Article |
| Sprache: | Unbestimmt |
| veröffentlicht: |
Association of Basic Medical Sciences of FBIH
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| Schlagwörter: |
| author_facet |
Zhang, Wei Huang, Lunhua Lu, Xinyang Wang, Kecheng Ning, Xiaofei Liu, Zhiqiang Zhang, Wei Huang, Lunhua Lu, Xinyang Wang, Kecheng Ning, Xiaofei Liu, Zhiqiang |
|---|---|
| author |
Zhang, Wei Huang, Lunhua Lu, Xinyang Wang, Kecheng Ning, Xiaofei Liu, Zhiqiang |
| spellingShingle |
Zhang, Wei Huang, Lunhua Lu, Xinyang Wang, Kecheng Ning, Xiaofei Liu, Zhiqiang Bosnian Journal of Basic Medical Sciences Upregulated expression of MNX1-AS1 long noncoding RNA predicts poor prognosis in gastric cancer General Medicine |
| author_sort |
zhang, wei |
| spelling |
Zhang, Wei Huang, Lunhua Lu, Xinyang Wang, Kecheng Ning, Xiaofei Liu, Zhiqiang 1840-4812 1512-8601 Association of Basic Medical Sciences of FBIH General Medicine http://dx.doi.org/10.17305/bjbms.2019.3713 <jats:p>As important regulators of gene expression long noncoding RNAs (lncRNAs) are implicated in various physiological and pathological processes, including cancer. An oncogenic role of MNX1 antisense RNA 1 (MNX1-AS1) lncRNA has been suggested in cervical cancer and glioblastoma. In this study, we investigated the clinicopathological significance and biological function of MNX1-AS1 in gastric cancer (GC). The expression of MNX1-AS1 was analyzed by qRT-PCR in 96 GC and adjacent non-tumor tissues in relation to clinicopathological features and overall survival (OS) of patients, and in five human GC cell lines compared to a normal gastric epithelial cell line. Loss-of-function experiments using small interfering RNA (siRNA) targeting MNX1-AS1 (si-MNX1-AS1) were carried out in AGS and MGC-803 GC cell lines. Cell proliferation (CCK-8 assay), migration (Transwell) and invasion (Transwell Matrigel), and protein expression of proliferating cell nuclear antigen (PCNA), E-cadherin, N-cadherin, vimentin and matrix metallopeptidase 9 (MMP-9) were analyzed in transfected GC cells. Expression of MNX1-AS1 was significantly higher in GC vs. adjacent non-tumor tissues. Higher MNX1-AS1 expression was significantly associated with tumor size, TNM stage and lymph node metastasis. Kaplan–Meier analysis showed that GC patients with higher MNX1-AS1 expression had worse OS compared to patients with lower MNX1-AS1 expression. Multivariate analysis showed that MNX1-AS1 is an independent poor prognostic factor in GC. Knockdown of MNX1-AS1 significantly inhibited proliferation, migration and invasion of AGS and MGC-803 cells, and resulted in increased E-cadherin and decreased PCNA, N-cadherin, vimentin and MMP-9 expression. Taken together, these results suggest that MNX1-AS1 has an oncogenic function in GC and potential as a molecular target in GC therapy.</jats:p> Upregulated expression of MNX1-AS1 long noncoding RNA predicts poor prognosis in gastric cancer Bosnian Journal of Basic Medical Sciences |
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Bosnian Journal of Basic Medical Sciences |
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| title |
Upregulated expression of MNX1-AS1 long noncoding RNA predicts poor prognosis in gastric cancer |
| title_unstemmed |
Upregulated expression of MNX1-AS1 long noncoding RNA predicts poor prognosis in gastric cancer |
| title_full |
Upregulated expression of MNX1-AS1 long noncoding RNA predicts poor prognosis in gastric cancer |
| title_fullStr |
Upregulated expression of MNX1-AS1 long noncoding RNA predicts poor prognosis in gastric cancer |
| title_full_unstemmed |
Upregulated expression of MNX1-AS1 long noncoding RNA predicts poor prognosis in gastric cancer |
| title_short |
Upregulated expression of MNX1-AS1 long noncoding RNA predicts poor prognosis in gastric cancer |
| title_sort |
upregulated expression of mnx1-as1 long noncoding rna predicts poor prognosis in gastric cancer |
| topic |
General Medicine |
| url |
http://dx.doi.org/10.17305/bjbms.2019.3713 |
| publishDate |
2019 |
| physical |
|
| description |
<jats:p>As important regulators of gene expression long noncoding RNAs (lncRNAs) are implicated in various physiological and pathological processes, including cancer. An oncogenic role of MNX1 antisense RNA 1 (MNX1-AS1) lncRNA has been suggested in cervical cancer and glioblastoma. In this study, we investigated the clinicopathological significance and biological function of MNX1-AS1 in gastric cancer (GC). The expression of MNX1-AS1 was analyzed by qRT-PCR in 96 GC and adjacent non-tumor tissues in relation to clinicopathological features and overall survival (OS) of patients, and in five human GC cell lines compared to a normal gastric epithelial cell line. Loss-of-function experiments using small interfering RNA (siRNA) targeting MNX1-AS1 (si-MNX1-AS1) were carried out in AGS and MGC-803 GC cell lines. Cell proliferation (CCK-8 assay), migration (Transwell) and invasion (Transwell Matrigel), and protein expression of proliferating cell nuclear antigen (PCNA), E-cadherin, N-cadherin, vimentin and matrix metallopeptidase 9 (MMP-9) were analyzed in transfected GC cells. Expression of MNX1-AS1 was significantly higher in GC vs. adjacent non-tumor tissues. Higher MNX1-AS1 expression was significantly associated with tumor size, TNM stage and lymph node metastasis. Kaplan–Meier analysis showed that GC patients with higher MNX1-AS1 expression had worse OS compared to patients with lower MNX1-AS1 expression. Multivariate analysis showed that MNX1-AS1 is an independent poor prognostic factor in GC. Knockdown of MNX1-AS1 significantly inhibited proliferation, migration and invasion of AGS and MGC-803 cells, and resulted in increased E-cadherin and decreased PCNA, N-cadherin, vimentin and MMP-9 expression. Taken together, these results suggest that MNX1-AS1 has an oncogenic function in GC and potential as a molecular target in GC therapy.</jats:p> |
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| author | Zhang, Wei, Huang, Lunhua, Lu, Xinyang, Wang, Kecheng, Ning, Xiaofei, Liu, Zhiqiang |
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| description | <jats:p>As important regulators of gene expression long noncoding RNAs (lncRNAs) are implicated in various physiological and pathological processes, including cancer. An oncogenic role of MNX1 antisense RNA 1 (MNX1-AS1) lncRNA has been suggested in cervical cancer and glioblastoma. In this study, we investigated the clinicopathological significance and biological function of MNX1-AS1 in gastric cancer (GC). The expression of MNX1-AS1 was analyzed by qRT-PCR in 96 GC and adjacent non-tumor tissues in relation to clinicopathological features and overall survival (OS) of patients, and in five human GC cell lines compared to a normal gastric epithelial cell line. Loss-of-function experiments using small interfering RNA (siRNA) targeting MNX1-AS1 (si-MNX1-AS1) were carried out in AGS and MGC-803 GC cell lines. Cell proliferation (CCK-8 assay), migration (Transwell) and invasion (Transwell Matrigel), and protein expression of proliferating cell nuclear antigen (PCNA), E-cadherin, N-cadherin, vimentin and matrix metallopeptidase 9 (MMP-9) were analyzed in transfected GC cells. Expression of MNX1-AS1 was significantly higher in GC vs. adjacent non-tumor tissues. Higher MNX1-AS1 expression was significantly associated with tumor size, TNM stage and lymph node metastasis. Kaplan–Meier analysis showed that GC patients with higher MNX1-AS1 expression had worse OS compared to patients with lower MNX1-AS1 expression. Multivariate analysis showed that MNX1-AS1 is an independent poor prognostic factor in GC. Knockdown of MNX1-AS1 significantly inhibited proliferation, migration and invasion of AGS and MGC-803 cells, and resulted in increased E-cadherin and decreased PCNA, N-cadherin, vimentin and MMP-9 expression. Taken together, these results suggest that MNX1-AS1 has an oncogenic function in GC and potential as a molecular target in GC therapy.</jats:p> |
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| spelling | Zhang, Wei Huang, Lunhua Lu, Xinyang Wang, Kecheng Ning, Xiaofei Liu, Zhiqiang 1840-4812 1512-8601 Association of Basic Medical Sciences of FBIH General Medicine http://dx.doi.org/10.17305/bjbms.2019.3713 <jats:p>As important regulators of gene expression long noncoding RNAs (lncRNAs) are implicated in various physiological and pathological processes, including cancer. An oncogenic role of MNX1 antisense RNA 1 (MNX1-AS1) lncRNA has been suggested in cervical cancer and glioblastoma. In this study, we investigated the clinicopathological significance and biological function of MNX1-AS1 in gastric cancer (GC). The expression of MNX1-AS1 was analyzed by qRT-PCR in 96 GC and adjacent non-tumor tissues in relation to clinicopathological features and overall survival (OS) of patients, and in five human GC cell lines compared to a normal gastric epithelial cell line. Loss-of-function experiments using small interfering RNA (siRNA) targeting MNX1-AS1 (si-MNX1-AS1) were carried out in AGS and MGC-803 GC cell lines. Cell proliferation (CCK-8 assay), migration (Transwell) and invasion (Transwell Matrigel), and protein expression of proliferating cell nuclear antigen (PCNA), E-cadherin, N-cadherin, vimentin and matrix metallopeptidase 9 (MMP-9) were analyzed in transfected GC cells. Expression of MNX1-AS1 was significantly higher in GC vs. adjacent non-tumor tissues. Higher MNX1-AS1 expression was significantly associated with tumor size, TNM stage and lymph node metastasis. Kaplan–Meier analysis showed that GC patients with higher MNX1-AS1 expression had worse OS compared to patients with lower MNX1-AS1 expression. Multivariate analysis showed that MNX1-AS1 is an independent poor prognostic factor in GC. Knockdown of MNX1-AS1 significantly inhibited proliferation, migration and invasion of AGS and MGC-803 cells, and resulted in increased E-cadherin and decreased PCNA, N-cadherin, vimentin and MMP-9 expression. Taken together, these results suggest that MNX1-AS1 has an oncogenic function in GC and potential as a molecular target in GC therapy.</jats:p> Upregulated expression of MNX1-AS1 long noncoding RNA predicts poor prognosis in gastric cancer Bosnian Journal of Basic Medical Sciences |
| spellingShingle | Zhang, Wei, Huang, Lunhua, Lu, Xinyang, Wang, Kecheng, Ning, Xiaofei, Liu, Zhiqiang, Bosnian Journal of Basic Medical Sciences, Upregulated expression of MNX1-AS1 long noncoding RNA predicts poor prognosis in gastric cancer, General Medicine |
| title | Upregulated expression of MNX1-AS1 long noncoding RNA predicts poor prognosis in gastric cancer |
| title_full | Upregulated expression of MNX1-AS1 long noncoding RNA predicts poor prognosis in gastric cancer |
| title_fullStr | Upregulated expression of MNX1-AS1 long noncoding RNA predicts poor prognosis in gastric cancer |
| title_full_unstemmed | Upregulated expression of MNX1-AS1 long noncoding RNA predicts poor prognosis in gastric cancer |
| title_short | Upregulated expression of MNX1-AS1 long noncoding RNA predicts poor prognosis in gastric cancer |
| title_sort | upregulated expression of mnx1-as1 long noncoding rna predicts poor prognosis in gastric cancer |
| title_unstemmed | Upregulated expression of MNX1-AS1 long noncoding RNA predicts poor prognosis in gastric cancer |
| topic | General Medicine |
| url | http://dx.doi.org/10.17305/bjbms.2019.3713 |