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Metabolic Changes in Mesenchymal Stem Cells in Osteogenic Medium Measured by Autofluorescence Spectroscopy
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| Zeitschriftentitel: | Stem Cells |
|---|---|
| Personen und Körperschaften: | , , , , , , , |
| In: | Stem Cells, 24, 2006, 5, S. 1213-1217 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Oxford University Press (OUP)
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| Schlagwörter: |
| author_facet |
Reyes, Johann M.G. Fermanian, Sara Yang, Fan Zhou, Shi-You Herretes, Samantha Murphy, Douglas B. Elisseeff, Jennifer H. Chuck, Roy S. Reyes, Johann M.G. Fermanian, Sara Yang, Fan Zhou, Shi-You Herretes, Samantha Murphy, Douglas B. Elisseeff, Jennifer H. Chuck, Roy S. |
|---|---|
| author |
Reyes, Johann M.G. Fermanian, Sara Yang, Fan Zhou, Shi-You Herretes, Samantha Murphy, Douglas B. Elisseeff, Jennifer H. Chuck, Roy S. |
| spellingShingle |
Reyes, Johann M.G. Fermanian, Sara Yang, Fan Zhou, Shi-You Herretes, Samantha Murphy, Douglas B. Elisseeff, Jennifer H. Chuck, Roy S. Stem Cells Metabolic Changes in Mesenchymal Stem Cells in Osteogenic Medium Measured by Autofluorescence Spectroscopy Cell Biology Developmental Biology Molecular Medicine |
| author_sort |
reyes, johann m.g. |
| spelling |
Reyes, Johann M.G. Fermanian, Sara Yang, Fan Zhou, Shi-You Herretes, Samantha Murphy, Douglas B. Elisseeff, Jennifer H. Chuck, Roy S. 1066-5099 1549-4918 Oxford University Press (OUP) Cell Biology Developmental Biology Molecular Medicine http://dx.doi.org/10.1634/stemcells.2004-0324 <jats:title>Abstract</jats:title> <jats:p>The purpose of this study was to measure metabolic changes in mesenchymal stem cells (MSCs) placed in osteogenic medium by autofluorescence spectroscopy. MSCs were plated in stem cell-supporting or osteogenic medium and imaged. Shift from the basic growth environment to the inductive osteogenic environment was confirmed by reverse transcription-polymerase chain reaction. Reduced pyridine nucleotides were detected by exciting near 366 nm and measuring fluorescence at 450 nm, and oxidized flavoproteins were detected by exciting at 460 nm and measuring fluorescence at 540 nm. The ratio of these fluorescence measurements, reduction-oxidation (redox) fluorometry, is a noninvasive measure of the cellular metabolic state. The detected pyridine nucleotide to flavoprotein ratio decreased upon transitioning from the stem cell to the differentiated state, as well as with increasing cell density and cell-cell contact. MSC metabolism increased upon placement in differentiating medium and with increasing cell density and contact. Redox fluorometry is a feasible, noninvasive technique for distinguishing MSCs from further differentiated cells.</jats:p> Metabolic Changes in Mesenchymal Stem Cells in Osteogenic Medium Measured by Autofluorescence Spectroscopy Stem Cells |
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| title |
Metabolic Changes in Mesenchymal Stem Cells in Osteogenic Medium Measured by Autofluorescence Spectroscopy |
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Metabolic Changes in Mesenchymal Stem Cells in Osteogenic Medium Measured by Autofluorescence Spectroscopy |
| title_full |
Metabolic Changes in Mesenchymal Stem Cells in Osteogenic Medium Measured by Autofluorescence Spectroscopy |
| title_fullStr |
Metabolic Changes in Mesenchymal Stem Cells in Osteogenic Medium Measured by Autofluorescence Spectroscopy |
| title_full_unstemmed |
Metabolic Changes in Mesenchymal Stem Cells in Osteogenic Medium Measured by Autofluorescence Spectroscopy |
| title_short |
Metabolic Changes in Mesenchymal Stem Cells in Osteogenic Medium Measured by Autofluorescence Spectroscopy |
| title_sort |
metabolic changes in mesenchymal stem cells in osteogenic medium measured by autofluorescence spectroscopy |
| topic |
Cell Biology Developmental Biology Molecular Medicine |
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http://dx.doi.org/10.1634/stemcells.2004-0324 |
| publishDate |
2006 |
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1213-1217 |
| description |
<jats:title>Abstract</jats:title>
<jats:p>The purpose of this study was to measure metabolic changes in mesenchymal stem cells (MSCs) placed in osteogenic medium by autofluorescence spectroscopy. MSCs were plated in stem cell-supporting or osteogenic medium and imaged. Shift from the basic growth environment to the inductive osteogenic environment was confirmed by reverse transcription-polymerase chain reaction. Reduced pyridine nucleotides were detected by exciting near 366 nm and measuring fluorescence at 450 nm, and oxidized flavoproteins were detected by exciting at 460 nm and measuring fluorescence at 540 nm. The ratio of these fluorescence measurements, reduction-oxidation (redox) fluorometry, is a noninvasive measure of the cellular metabolic state. The detected pyridine nucleotide to flavoprotein ratio decreased upon transitioning from the stem cell to the differentiated state, as well as with increasing cell density and cell-cell contact. MSC metabolism increased upon placement in differentiating medium and with increasing cell density and contact. Redox fluorometry is a feasible, noninvasive technique for distinguishing MSCs from further differentiated cells.</jats:p> |
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| author | Reyes, Johann M.G., Fermanian, Sara, Yang, Fan, Zhou, Shi-You, Herretes, Samantha, Murphy, Douglas B., Elisseeff, Jennifer H., Chuck, Roy S. |
| author_facet | Reyes, Johann M.G., Fermanian, Sara, Yang, Fan, Zhou, Shi-You, Herretes, Samantha, Murphy, Douglas B., Elisseeff, Jennifer H., Chuck, Roy S., Reyes, Johann M.G., Fermanian, Sara, Yang, Fan, Zhou, Shi-You, Herretes, Samantha, Murphy, Douglas B., Elisseeff, Jennifer H., Chuck, Roy S. |
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| description | <jats:title>Abstract</jats:title> <jats:p>The purpose of this study was to measure metabolic changes in mesenchymal stem cells (MSCs) placed in osteogenic medium by autofluorescence spectroscopy. MSCs were plated in stem cell-supporting or osteogenic medium and imaged. Shift from the basic growth environment to the inductive osteogenic environment was confirmed by reverse transcription-polymerase chain reaction. Reduced pyridine nucleotides were detected by exciting near 366 nm and measuring fluorescence at 450 nm, and oxidized flavoproteins were detected by exciting at 460 nm and measuring fluorescence at 540 nm. The ratio of these fluorescence measurements, reduction-oxidation (redox) fluorometry, is a noninvasive measure of the cellular metabolic state. The detected pyridine nucleotide to flavoprotein ratio decreased upon transitioning from the stem cell to the differentiated state, as well as with increasing cell density and cell-cell contact. MSC metabolism increased upon placement in differentiating medium and with increasing cell density and contact. Redox fluorometry is a feasible, noninvasive technique for distinguishing MSCs from further differentiated cells.</jats:p> |
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| spelling | Reyes, Johann M.G. Fermanian, Sara Yang, Fan Zhou, Shi-You Herretes, Samantha Murphy, Douglas B. Elisseeff, Jennifer H. Chuck, Roy S. 1066-5099 1549-4918 Oxford University Press (OUP) Cell Biology Developmental Biology Molecular Medicine http://dx.doi.org/10.1634/stemcells.2004-0324 <jats:title>Abstract</jats:title> <jats:p>The purpose of this study was to measure metabolic changes in mesenchymal stem cells (MSCs) placed in osteogenic medium by autofluorescence spectroscopy. MSCs were plated in stem cell-supporting or osteogenic medium and imaged. Shift from the basic growth environment to the inductive osteogenic environment was confirmed by reverse transcription-polymerase chain reaction. Reduced pyridine nucleotides were detected by exciting near 366 nm and measuring fluorescence at 450 nm, and oxidized flavoproteins were detected by exciting at 460 nm and measuring fluorescence at 540 nm. The ratio of these fluorescence measurements, reduction-oxidation (redox) fluorometry, is a noninvasive measure of the cellular metabolic state. The detected pyridine nucleotide to flavoprotein ratio decreased upon transitioning from the stem cell to the differentiated state, as well as with increasing cell density and cell-cell contact. MSC metabolism increased upon placement in differentiating medium and with increasing cell density and contact. Redox fluorometry is a feasible, noninvasive technique for distinguishing MSCs from further differentiated cells.</jats:p> Metabolic Changes in Mesenchymal Stem Cells in Osteogenic Medium Measured by Autofluorescence Spectroscopy Stem Cells |
| spellingShingle | Reyes, Johann M.G., Fermanian, Sara, Yang, Fan, Zhou, Shi-You, Herretes, Samantha, Murphy, Douglas B., Elisseeff, Jennifer H., Chuck, Roy S., Stem Cells, Metabolic Changes in Mesenchymal Stem Cells in Osteogenic Medium Measured by Autofluorescence Spectroscopy, Cell Biology, Developmental Biology, Molecular Medicine |
| title | Metabolic Changes in Mesenchymal Stem Cells in Osteogenic Medium Measured by Autofluorescence Spectroscopy |
| title_full | Metabolic Changes in Mesenchymal Stem Cells in Osteogenic Medium Measured by Autofluorescence Spectroscopy |
| title_fullStr | Metabolic Changes in Mesenchymal Stem Cells in Osteogenic Medium Measured by Autofluorescence Spectroscopy |
| title_full_unstemmed | Metabolic Changes in Mesenchymal Stem Cells in Osteogenic Medium Measured by Autofluorescence Spectroscopy |
| title_short | Metabolic Changes in Mesenchymal Stem Cells in Osteogenic Medium Measured by Autofluorescence Spectroscopy |
| title_sort | metabolic changes in mesenchymal stem cells in osteogenic medium measured by autofluorescence spectroscopy |
| title_unstemmed | Metabolic Changes in Mesenchymal Stem Cells in Osteogenic Medium Measured by Autofluorescence Spectroscopy |
| topic | Cell Biology, Developmental Biology, Molecular Medicine |
| url | http://dx.doi.org/10.1634/stemcells.2004-0324 |