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Presynaptic Glycine Receptors on GABAergic Terminals Facilitate Discharge of Dopaminergic Neurons in Ventral Tegmental Area
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| Zeitschriftentitel: | The Journal of Neuroscience |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | The Journal of Neuroscience, 24, 2004, 41, S. 8961-8974 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Society for Neuroscience
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| Schlagwörter: |
| author_facet |
Ye, Jiang-Hong Wang, Fushun Krnjević, Kresimir Wang, Weizhen Xiong, Zhi-Gang Zhang, Jingli Ye, Jiang-Hong Wang, Fushun Krnjević, Kresimir Wang, Weizhen Xiong, Zhi-Gang Zhang, Jingli |
|---|---|
| author |
Ye, Jiang-Hong Wang, Fushun Krnjević, Kresimir Wang, Weizhen Xiong, Zhi-Gang Zhang, Jingli |
| spellingShingle |
Ye, Jiang-Hong Wang, Fushun Krnjević, Kresimir Wang, Weizhen Xiong, Zhi-Gang Zhang, Jingli The Journal of Neuroscience Presynaptic Glycine Receptors on GABAergic Terminals Facilitate Discharge of Dopaminergic Neurons in Ventral Tegmental Area General Neuroscience |
| author_sort |
ye, jiang-hong |
| spelling |
Ye, Jiang-Hong Wang, Fushun Krnjević, Kresimir Wang, Weizhen Xiong, Zhi-Gang Zhang, Jingli 0270-6474 1529-2401 Society for Neuroscience General Neuroscience http://dx.doi.org/10.1523/jneurosci.2016-04.2004 <jats:p>GABA-mediated postsynaptic currents (IPSCs) were recorded from dopaminergic (DA) neurons of the ventral tegmental area (VTA) of rats, in acute brain slices, and from enzymatically or mechanically dissociated neurons. In young rats (3-10 d of age), where GABA is excitatory, glycine (1-3 μ<jats:sc>m</jats:sc>) and taurine (10-30 μ<jats:sc>m</jats:sc>) increased the amplitude of evoked IPSCs (eIPSCs) and the frequency of spontaneous IPSCs (sIPSCs) but had minimal postsynaptic effects. Strychnine (1 μ<jats:sc>m</jats:sc>) blocked the action of glycine; when applied alone, it reduced the amplitude of eIPSCs and the frequency of sIPSCs, indicating a tonic facilitation of GABAergic excitation by some endogenous glycine agonist(s). In medium containing no Ca<jats:sup>2+</jats:sup>, or with Cd<jats:sup>2+</jats:sup>or tetrodotoxin added, the amplitude and especially the frequency of sIPSCs greatly diminished. In many cells, glycine had no effect on remaining miniature IPSCs, suggesting a preterminal site of glycine receptors (GlyRs). Fura-2 fluorescent imaging showed a glycine-induced increase of [Ca<jats:sup>2+</jats:sup>] in nerve terminals (on DA neurons), which was suppressed by strychnine or 3 μ<jats:sc>m</jats:sc>ω-conotoxin MVIIA. Therefore, the presynaptic GlyR-mediated facilitation of GABAergic transmission seems to be mediated by N- and/or P/Q-type Ca<jats:sup>2+</jats:sup>channels. In older rats (22-30 d of age), where GABA causes inhibition, the effect of strychnine on GABAergic IPSCs was reversed to facilitation, indicating a tonic glycinergic inhibition of GABA release. Furthermore, glycine (1-3 μ<jats:sc>m</jats:sc>) reduced the amplitude of eIPSCs and the frequency of sIPSCs. Hence, the overall effect of the presynaptic action of glycine is to enhance the firing of DA cells, both in very young and older rats.</jats:p> Presynaptic Glycine Receptors on GABAergic Terminals Facilitate Discharge of Dopaminergic Neurons in Ventral Tegmental Area The Journal of Neuroscience |
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10.1523/jneurosci.2016-04.2004 |
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Online Free |
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ElectronicArticle |
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Society for Neuroscience, 2004 |
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Society for Neuroscience, 2004 |
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0270-6474 1529-2401 |
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0270-6474 1529-2401 |
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ye2004presynapticglycinereceptorsongabaergicterminalsfacilitatedischargeofdopaminergicneuronsinventraltegmentalarea |
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2004 |
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Society for Neuroscience |
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ai |
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The Journal of Neuroscience |
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49 |
| title |
Presynaptic Glycine Receptors on GABAergic Terminals Facilitate Discharge of Dopaminergic Neurons in Ventral Tegmental Area |
| title_unstemmed |
Presynaptic Glycine Receptors on GABAergic Terminals Facilitate Discharge of Dopaminergic Neurons in Ventral Tegmental Area |
| title_full |
Presynaptic Glycine Receptors on GABAergic Terminals Facilitate Discharge of Dopaminergic Neurons in Ventral Tegmental Area |
| title_fullStr |
Presynaptic Glycine Receptors on GABAergic Terminals Facilitate Discharge of Dopaminergic Neurons in Ventral Tegmental Area |
| title_full_unstemmed |
Presynaptic Glycine Receptors on GABAergic Terminals Facilitate Discharge of Dopaminergic Neurons in Ventral Tegmental Area |
| title_short |
Presynaptic Glycine Receptors on GABAergic Terminals Facilitate Discharge of Dopaminergic Neurons in Ventral Tegmental Area |
| title_sort |
presynaptic glycine receptors on gabaergic terminals facilitate discharge of dopaminergic neurons in ventral tegmental area |
| topic |
General Neuroscience |
| url |
http://dx.doi.org/10.1523/jneurosci.2016-04.2004 |
| publishDate |
2004 |
| physical |
8961-8974 |
| description |
<jats:p>GABA-mediated postsynaptic currents (IPSCs) were recorded from dopaminergic (DA) neurons of the ventral tegmental area (VTA) of rats, in acute brain slices, and from enzymatically or mechanically dissociated neurons. In young rats (3-10 d of age), where GABA is excitatory, glycine (1-3 μ<jats:sc>m</jats:sc>) and taurine (10-30 μ<jats:sc>m</jats:sc>) increased the amplitude of evoked IPSCs (eIPSCs) and the frequency of spontaneous IPSCs (sIPSCs) but had minimal postsynaptic effects. Strychnine (1 μ<jats:sc>m</jats:sc>) blocked the action of glycine; when applied alone, it reduced the amplitude of eIPSCs and the frequency of sIPSCs, indicating a tonic facilitation of GABAergic excitation by some endogenous glycine agonist(s). In medium containing no Ca<jats:sup>2+</jats:sup>, or with Cd<jats:sup>2+</jats:sup>or tetrodotoxin added, the amplitude and especially the frequency of sIPSCs greatly diminished. In many cells, glycine had no effect on remaining miniature IPSCs, suggesting a preterminal site of glycine receptors (GlyRs). Fura-2 fluorescent imaging showed a glycine-induced increase of [Ca<jats:sup>2+</jats:sup>] in nerve terminals (on DA neurons), which was suppressed by strychnine or 3 μ<jats:sc>m</jats:sc>ω-conotoxin MVIIA. Therefore, the presynaptic GlyR-mediated facilitation of GABAergic transmission seems to be mediated by N- and/or P/Q-type Ca<jats:sup>2+</jats:sup>channels. In older rats (22-30 d of age), where GABA causes inhibition, the effect of strychnine on GABAergic IPSCs was reversed to facilitation, indicating a tonic glycinergic inhibition of GABA release. Furthermore, glycine (1-3 μ<jats:sc>m</jats:sc>) reduced the amplitude of eIPSCs and the frequency of sIPSCs. Hence, the overall effect of the presynaptic action of glycine is to enhance the firing of DA cells, both in very young and older rats.</jats:p> |
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| author | Ye, Jiang-Hong, Wang, Fushun, Krnjević, Kresimir, Wang, Weizhen, Xiong, Zhi-Gang, Zhang, Jingli |
| author_facet | Ye, Jiang-Hong, Wang, Fushun, Krnjević, Kresimir, Wang, Weizhen, Xiong, Zhi-Gang, Zhang, Jingli, Ye, Jiang-Hong, Wang, Fushun, Krnjević, Kresimir, Wang, Weizhen, Xiong, Zhi-Gang, Zhang, Jingli |
| author_sort | ye, jiang-hong |
| container_issue | 41 |
| container_start_page | 8961 |
| container_title | The Journal of Neuroscience |
| container_volume | 24 |
| description | <jats:p>GABA-mediated postsynaptic currents (IPSCs) were recorded from dopaminergic (DA) neurons of the ventral tegmental area (VTA) of rats, in acute brain slices, and from enzymatically or mechanically dissociated neurons. In young rats (3-10 d of age), where GABA is excitatory, glycine (1-3 μ<jats:sc>m</jats:sc>) and taurine (10-30 μ<jats:sc>m</jats:sc>) increased the amplitude of evoked IPSCs (eIPSCs) and the frequency of spontaneous IPSCs (sIPSCs) but had minimal postsynaptic effects. Strychnine (1 μ<jats:sc>m</jats:sc>) blocked the action of glycine; when applied alone, it reduced the amplitude of eIPSCs and the frequency of sIPSCs, indicating a tonic facilitation of GABAergic excitation by some endogenous glycine agonist(s). In medium containing no Ca<jats:sup>2+</jats:sup>, or with Cd<jats:sup>2+</jats:sup>or tetrodotoxin added, the amplitude and especially the frequency of sIPSCs greatly diminished. In many cells, glycine had no effect on remaining miniature IPSCs, suggesting a preterminal site of glycine receptors (GlyRs). Fura-2 fluorescent imaging showed a glycine-induced increase of [Ca<jats:sup>2+</jats:sup>] in nerve terminals (on DA neurons), which was suppressed by strychnine or 3 μ<jats:sc>m</jats:sc>ω-conotoxin MVIIA. Therefore, the presynaptic GlyR-mediated facilitation of GABAergic transmission seems to be mediated by N- and/or P/Q-type Ca<jats:sup>2+</jats:sup>channels. In older rats (22-30 d of age), where GABA causes inhibition, the effect of strychnine on GABAergic IPSCs was reversed to facilitation, indicating a tonic glycinergic inhibition of GABA release. Furthermore, glycine (1-3 μ<jats:sc>m</jats:sc>) reduced the amplitude of eIPSCs and the frequency of sIPSCs. Hence, the overall effect of the presynaptic action of glycine is to enhance the firing of DA cells, both in very young and older rats.</jats:p> |
| doi_str_mv | 10.1523/jneurosci.2016-04.2004 |
| facet_avail | Online, Free |
| format | ElectronicArticle |
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| imprint | Society for Neuroscience, 2004 |
| imprint_str_mv | Society for Neuroscience, 2004 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
| issn | 0270-6474, 1529-2401 |
| issn_str_mv | 0270-6474, 1529-2401 |
| language | English |
| last_indexed | 2024-03-01T15:37:30.78Z |
| match_str | ye2004presynapticglycinereceptorsongabaergicterminalsfacilitatedischargeofdopaminergicneuronsinventraltegmentalarea |
| mega_collection | Society for Neuroscience (CrossRef) |
| physical | 8961-8974 |
| publishDate | 2004 |
| publishDateSort | 2004 |
| publisher | Society for Neuroscience |
| record_format | ai |
| recordtype | ai |
| series | The Journal of Neuroscience |
| source_id | 49 |
| spelling | Ye, Jiang-Hong Wang, Fushun Krnjević, Kresimir Wang, Weizhen Xiong, Zhi-Gang Zhang, Jingli 0270-6474 1529-2401 Society for Neuroscience General Neuroscience http://dx.doi.org/10.1523/jneurosci.2016-04.2004 <jats:p>GABA-mediated postsynaptic currents (IPSCs) were recorded from dopaminergic (DA) neurons of the ventral tegmental area (VTA) of rats, in acute brain slices, and from enzymatically or mechanically dissociated neurons. In young rats (3-10 d of age), where GABA is excitatory, glycine (1-3 μ<jats:sc>m</jats:sc>) and taurine (10-30 μ<jats:sc>m</jats:sc>) increased the amplitude of evoked IPSCs (eIPSCs) and the frequency of spontaneous IPSCs (sIPSCs) but had minimal postsynaptic effects. Strychnine (1 μ<jats:sc>m</jats:sc>) blocked the action of glycine; when applied alone, it reduced the amplitude of eIPSCs and the frequency of sIPSCs, indicating a tonic facilitation of GABAergic excitation by some endogenous glycine agonist(s). In medium containing no Ca<jats:sup>2+</jats:sup>, or with Cd<jats:sup>2+</jats:sup>or tetrodotoxin added, the amplitude and especially the frequency of sIPSCs greatly diminished. In many cells, glycine had no effect on remaining miniature IPSCs, suggesting a preterminal site of glycine receptors (GlyRs). Fura-2 fluorescent imaging showed a glycine-induced increase of [Ca<jats:sup>2+</jats:sup>] in nerve terminals (on DA neurons), which was suppressed by strychnine or 3 μ<jats:sc>m</jats:sc>ω-conotoxin MVIIA. Therefore, the presynaptic GlyR-mediated facilitation of GABAergic transmission seems to be mediated by N- and/or P/Q-type Ca<jats:sup>2+</jats:sup>channels. In older rats (22-30 d of age), where GABA causes inhibition, the effect of strychnine on GABAergic IPSCs was reversed to facilitation, indicating a tonic glycinergic inhibition of GABA release. Furthermore, glycine (1-3 μ<jats:sc>m</jats:sc>) reduced the amplitude of eIPSCs and the frequency of sIPSCs. Hence, the overall effect of the presynaptic action of glycine is to enhance the firing of DA cells, both in very young and older rats.</jats:p> Presynaptic Glycine Receptors on GABAergic Terminals Facilitate Discharge of Dopaminergic Neurons in Ventral Tegmental Area The Journal of Neuroscience |
| spellingShingle | Ye, Jiang-Hong, Wang, Fushun, Krnjević, Kresimir, Wang, Weizhen, Xiong, Zhi-Gang, Zhang, Jingli, The Journal of Neuroscience, Presynaptic Glycine Receptors on GABAergic Terminals Facilitate Discharge of Dopaminergic Neurons in Ventral Tegmental Area, General Neuroscience |
| title | Presynaptic Glycine Receptors on GABAergic Terminals Facilitate Discharge of Dopaminergic Neurons in Ventral Tegmental Area |
| title_full | Presynaptic Glycine Receptors on GABAergic Terminals Facilitate Discharge of Dopaminergic Neurons in Ventral Tegmental Area |
| title_fullStr | Presynaptic Glycine Receptors on GABAergic Terminals Facilitate Discharge of Dopaminergic Neurons in Ventral Tegmental Area |
| title_full_unstemmed | Presynaptic Glycine Receptors on GABAergic Terminals Facilitate Discharge of Dopaminergic Neurons in Ventral Tegmental Area |
| title_short | Presynaptic Glycine Receptors on GABAergic Terminals Facilitate Discharge of Dopaminergic Neurons in Ventral Tegmental Area |
| title_sort | presynaptic glycine receptors on gabaergic terminals facilitate discharge of dopaminergic neurons in ventral tegmental area |
| title_unstemmed | Presynaptic Glycine Receptors on GABAergic Terminals Facilitate Discharge of Dopaminergic Neurons in Ventral Tegmental Area |
| topic | General Neuroscience |
| url | http://dx.doi.org/10.1523/jneurosci.2016-04.2004 |