Eintrag weiter verarbeiten
Flotillin-Mediated Endocytic Events Dictate Cell Type-Specific Responses to Semaphorin 3A
Gespeichert in:
| Zeitschriftentitel: | The Journal of Neuroscience |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | The Journal of Neuroscience, 30, 2010, 45, S. 15317-15329 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Society for Neuroscience
|
| Schlagwörter: |
| author_facet |
Carcea, Ioana Ma'ayan, Avi Mesias, Roxana Sepulveda, Bryan Salton, Stephen R. Benson, Deanna L. Carcea, Ioana Ma'ayan, Avi Mesias, Roxana Sepulveda, Bryan Salton, Stephen R. Benson, Deanna L. |
|---|---|
| author |
Carcea, Ioana Ma'ayan, Avi Mesias, Roxana Sepulveda, Bryan Salton, Stephen R. Benson, Deanna L. |
| spellingShingle |
Carcea, Ioana Ma'ayan, Avi Mesias, Roxana Sepulveda, Bryan Salton, Stephen R. Benson, Deanna L. The Journal of Neuroscience Flotillin-Mediated Endocytic Events Dictate Cell Type-Specific Responses to Semaphorin 3A General Neuroscience |
| author_sort |
carcea, ioana |
| spelling |
Carcea, Ioana Ma'ayan, Avi Mesias, Roxana Sepulveda, Bryan Salton, Stephen R. Benson, Deanna L. 0270-6474 1529-2401 Society for Neuroscience General Neuroscience http://dx.doi.org/10.1523/jneurosci.1821-10.2010 <jats:p>Cortical efferents growing in the same environment diverge early in development. The expression of particular transcription factors dictates the trajectories taken, presumably by regulating responsiveness to guidance cues via cellular mechanisms that are not yet known. Here, we show that cortical neurons that are dissociated and grown in culture maintain their cell type-specific identities defined by the expression of transcription factors. Using this model system, we sought to identify and characterize mechanisms that are recruited to produce cell type-specific responses to Semaphorin 3A (Sema3A), a guidance cue that would be presented similarly to cortical axons<jats:italic>in vivo</jats:italic>. Axons from presumptive corticofugal neurons lacking the transcription factor Satb2 and expressing Ctip2 or Tbr1 respond far more robustly to Sema3A than those from presumptive callosal neurons expressing Satb2. Both populations of axons express similar levels of Sema3A receptors (neuropilin-1, cell adhesion molecule L1, and plexinA4), but significantly, axons from neurons lacking Satb2 internalize more Sema3A, and they do so via a raft-mediated endocytic pathway. We used an<jats:italic>in silico</jats:italic>approach to identify the endocytosis effector flotillin-1 as a Sema3A signaling candidate. We tested the contributions of flotillin-1 to Sema3A endocytosis and signaling, and show that raft-mediated Sema3A endocytosis is defined by and depends on the recruitment of flotillin-1, which mediates LIM domain kinase activation and regulates axon responsiveness to Sema3A in presumptive corticofugal axons.</jats:p> Flotillin-Mediated Endocytic Events Dictate Cell Type-Specific Responses to Semaphorin 3A The Journal of Neuroscience |
| doi_str_mv |
10.1523/jneurosci.1821-10.2010 |
| facet_avail |
Online Free |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTUyMy9qbmV1cm9zY2kuMTgyMS0xMC4yMDEw |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTUyMy9qbmV1cm9zY2kuMTgyMS0xMC4yMDEw |
| institution |
DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 |
| imprint |
Society for Neuroscience, 2010 |
| imprint_str_mv |
Society for Neuroscience, 2010 |
| issn |
0270-6474 1529-2401 |
| issn_str_mv |
0270-6474 1529-2401 |
| language |
English |
| mega_collection |
Society for Neuroscience (CrossRef) |
| match_str |
carcea2010flotillinmediatedendocyticeventsdictatecelltypespecificresponsestosemaphorin3a |
| publishDateSort |
2010 |
| publisher |
Society for Neuroscience |
| recordtype |
ai |
| record_format |
ai |
| series |
The Journal of Neuroscience |
| source_id |
49 |
| title |
Flotillin-Mediated Endocytic Events Dictate Cell Type-Specific Responses to Semaphorin 3A |
| title_unstemmed |
Flotillin-Mediated Endocytic Events Dictate Cell Type-Specific Responses to Semaphorin 3A |
| title_full |
Flotillin-Mediated Endocytic Events Dictate Cell Type-Specific Responses to Semaphorin 3A |
| title_fullStr |
Flotillin-Mediated Endocytic Events Dictate Cell Type-Specific Responses to Semaphorin 3A |
| title_full_unstemmed |
Flotillin-Mediated Endocytic Events Dictate Cell Type-Specific Responses to Semaphorin 3A |
| title_short |
Flotillin-Mediated Endocytic Events Dictate Cell Type-Specific Responses to Semaphorin 3A |
| title_sort |
flotillin-mediated endocytic events dictate cell type-specific responses to semaphorin 3a |
| topic |
General Neuroscience |
| url |
http://dx.doi.org/10.1523/jneurosci.1821-10.2010 |
| publishDate |
2010 |
| physical |
15317-15329 |
| description |
<jats:p>Cortical efferents growing in the same environment diverge early in development. The expression of particular transcription factors dictates the trajectories taken, presumably by regulating responsiveness to guidance cues via cellular mechanisms that are not yet known. Here, we show that cortical neurons that are dissociated and grown in culture maintain their cell type-specific identities defined by the expression of transcription factors. Using this model system, we sought to identify and characterize mechanisms that are recruited to produce cell type-specific responses to Semaphorin 3A (Sema3A), a guidance cue that would be presented similarly to cortical axons<jats:italic>in vivo</jats:italic>. Axons from presumptive corticofugal neurons lacking the transcription factor Satb2 and expressing Ctip2 or Tbr1 respond far more robustly to Sema3A than those from presumptive callosal neurons expressing Satb2. Both populations of axons express similar levels of Sema3A receptors (neuropilin-1, cell adhesion molecule L1, and plexinA4), but significantly, axons from neurons lacking Satb2 internalize more Sema3A, and they do so via a raft-mediated endocytic pathway. We used an<jats:italic>in silico</jats:italic>approach to identify the endocytosis effector flotillin-1 as a Sema3A signaling candidate. We tested the contributions of flotillin-1 to Sema3A endocytosis and signaling, and show that raft-mediated Sema3A endocytosis is defined by and depends on the recruitment of flotillin-1, which mediates LIM domain kinase activation and regulates axon responsiveness to Sema3A in presumptive corticofugal axons.</jats:p> |
| container_issue |
45 |
| container_start_page |
15317 |
| container_title |
The Journal of Neuroscience |
| container_volume |
30 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792342794556145673 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T16:41:29.1Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Flotillin-Mediated+Endocytic+Events+Dictate+Cell+Type-Specific+Responses+to+Semaphorin+3A&rft.date=2010-11-10&genre=article&issn=1529-2401&volume=30&issue=45&spage=15317&epage=15329&pages=15317-15329&jtitle=The+Journal+of+Neuroscience&atitle=Flotillin-Mediated+Endocytic+Events+Dictate+Cell+Type-Specific+Responses+to+Semaphorin+3A&aulast=Benson&aufirst=Deanna+L.&rft_id=info%3Adoi%2F10.1523%2Fjneurosci.1821-10.2010&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792342794556145673 |
| author | Carcea, Ioana, Ma'ayan, Avi, Mesias, Roxana, Sepulveda, Bryan, Salton, Stephen R., Benson, Deanna L. |
| author_facet | Carcea, Ioana, Ma'ayan, Avi, Mesias, Roxana, Sepulveda, Bryan, Salton, Stephen R., Benson, Deanna L., Carcea, Ioana, Ma'ayan, Avi, Mesias, Roxana, Sepulveda, Bryan, Salton, Stephen R., Benson, Deanna L. |
| author_sort | carcea, ioana |
| container_issue | 45 |
| container_start_page | 15317 |
| container_title | The Journal of Neuroscience |
| container_volume | 30 |
| description | <jats:p>Cortical efferents growing in the same environment diverge early in development. The expression of particular transcription factors dictates the trajectories taken, presumably by regulating responsiveness to guidance cues via cellular mechanisms that are not yet known. Here, we show that cortical neurons that are dissociated and grown in culture maintain their cell type-specific identities defined by the expression of transcription factors. Using this model system, we sought to identify and characterize mechanisms that are recruited to produce cell type-specific responses to Semaphorin 3A (Sema3A), a guidance cue that would be presented similarly to cortical axons<jats:italic>in vivo</jats:italic>. Axons from presumptive corticofugal neurons lacking the transcription factor Satb2 and expressing Ctip2 or Tbr1 respond far more robustly to Sema3A than those from presumptive callosal neurons expressing Satb2. Both populations of axons express similar levels of Sema3A receptors (neuropilin-1, cell adhesion molecule L1, and plexinA4), but significantly, axons from neurons lacking Satb2 internalize more Sema3A, and they do so via a raft-mediated endocytic pathway. We used an<jats:italic>in silico</jats:italic>approach to identify the endocytosis effector flotillin-1 as a Sema3A signaling candidate. We tested the contributions of flotillin-1 to Sema3A endocytosis and signaling, and show that raft-mediated Sema3A endocytosis is defined by and depends on the recruitment of flotillin-1, which mediates LIM domain kinase activation and regulates axon responsiveness to Sema3A in presumptive corticofugal axons.</jats:p> |
| doi_str_mv | 10.1523/jneurosci.1821-10.2010 |
| facet_avail | Online, Free |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTUyMy9qbmV1cm9zY2kuMTgyMS0xMC4yMDEw |
| imprint | Society for Neuroscience, 2010 |
| imprint_str_mv | Society for Neuroscience, 2010 |
| institution | DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1 |
| issn | 0270-6474, 1529-2401 |
| issn_str_mv | 0270-6474, 1529-2401 |
| language | English |
| last_indexed | 2024-03-01T16:41:29.1Z |
| match_str | carcea2010flotillinmediatedendocyticeventsdictatecelltypespecificresponsestosemaphorin3a |
| mega_collection | Society for Neuroscience (CrossRef) |
| physical | 15317-15329 |
| publishDate | 2010 |
| publishDateSort | 2010 |
| publisher | Society for Neuroscience |
| record_format | ai |
| recordtype | ai |
| series | The Journal of Neuroscience |
| source_id | 49 |
| spelling | Carcea, Ioana Ma'ayan, Avi Mesias, Roxana Sepulveda, Bryan Salton, Stephen R. Benson, Deanna L. 0270-6474 1529-2401 Society for Neuroscience General Neuroscience http://dx.doi.org/10.1523/jneurosci.1821-10.2010 <jats:p>Cortical efferents growing in the same environment diverge early in development. The expression of particular transcription factors dictates the trajectories taken, presumably by regulating responsiveness to guidance cues via cellular mechanisms that are not yet known. Here, we show that cortical neurons that are dissociated and grown in culture maintain their cell type-specific identities defined by the expression of transcription factors. Using this model system, we sought to identify and characterize mechanisms that are recruited to produce cell type-specific responses to Semaphorin 3A (Sema3A), a guidance cue that would be presented similarly to cortical axons<jats:italic>in vivo</jats:italic>. Axons from presumptive corticofugal neurons lacking the transcription factor Satb2 and expressing Ctip2 or Tbr1 respond far more robustly to Sema3A than those from presumptive callosal neurons expressing Satb2. Both populations of axons express similar levels of Sema3A receptors (neuropilin-1, cell adhesion molecule L1, and plexinA4), but significantly, axons from neurons lacking Satb2 internalize more Sema3A, and they do so via a raft-mediated endocytic pathway. We used an<jats:italic>in silico</jats:italic>approach to identify the endocytosis effector flotillin-1 as a Sema3A signaling candidate. We tested the contributions of flotillin-1 to Sema3A endocytosis and signaling, and show that raft-mediated Sema3A endocytosis is defined by and depends on the recruitment of flotillin-1, which mediates LIM domain kinase activation and regulates axon responsiveness to Sema3A in presumptive corticofugal axons.</jats:p> Flotillin-Mediated Endocytic Events Dictate Cell Type-Specific Responses to Semaphorin 3A The Journal of Neuroscience |
| spellingShingle | Carcea, Ioana, Ma'ayan, Avi, Mesias, Roxana, Sepulveda, Bryan, Salton, Stephen R., Benson, Deanna L., The Journal of Neuroscience, Flotillin-Mediated Endocytic Events Dictate Cell Type-Specific Responses to Semaphorin 3A, General Neuroscience |
| title | Flotillin-Mediated Endocytic Events Dictate Cell Type-Specific Responses to Semaphorin 3A |
| title_full | Flotillin-Mediated Endocytic Events Dictate Cell Type-Specific Responses to Semaphorin 3A |
| title_fullStr | Flotillin-Mediated Endocytic Events Dictate Cell Type-Specific Responses to Semaphorin 3A |
| title_full_unstemmed | Flotillin-Mediated Endocytic Events Dictate Cell Type-Specific Responses to Semaphorin 3A |
| title_short | Flotillin-Mediated Endocytic Events Dictate Cell Type-Specific Responses to Semaphorin 3A |
| title_sort | flotillin-mediated endocytic events dictate cell type-specific responses to semaphorin 3a |
| title_unstemmed | Flotillin-Mediated Endocytic Events Dictate Cell Type-Specific Responses to Semaphorin 3A |
| topic | General Neuroscience |
| url | http://dx.doi.org/10.1523/jneurosci.1821-10.2010 |