Eintrag weiter verarbeiten
Desensitization of the neuronal 5-HT carrier following its long-term blockade
Gespeichert in:
| Zeitschriftentitel: | The Journal of Neuroscience |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | The Journal of Neuroscience, 14, 1994, 5, S. 3036-3047 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Society for Neuroscience
|
| Schlagwörter: |
| author_facet |
Pineyro, G Blier, P Dennis, T de Montigny, C Pineyro, G Blier, P Dennis, T de Montigny, C |
|---|---|
| author |
Pineyro, G Blier, P Dennis, T de Montigny, C |
| spellingShingle |
Pineyro, G Blier, P Dennis, T de Montigny, C The Journal of Neuroscience Desensitization of the neuronal 5-HT carrier following its long-term blockade General Neuroscience |
| author_sort |
pineyro, g |
| spelling |
Pineyro, G Blier, P Dennis, T de Montigny, C 0270-6474 1529-2401 Society for Neuroscience General Neuroscience http://dx.doi.org/10.1523/jneurosci.14-05-03036.1994 <jats:p>In vivo extracellular unitary recordings, in vitro 3H-5-hydroxy- tryptamine (5-HT) uptake, and 3H-paroxetine binding assays were used to assess the effect of acute and long-term administration of the 5-HT reuptake inhibitor paroxetine on the neuronal 5-HT transporter in the rat dorsal hippocampus. Recovery time of the firing activity of CA3 hippocampus pyramidal neurons following microiontophoretic application of 5-HT was used as an index of in vivo reuptake activity. In a first series of experiments, the acute intravenous administration of paroxetine and 5-HT denervation with the neurotoxin 5,7- dihydroxytryptamine produced a marked prolongation of the suppressant effect of 5-HT, indicating that reuptake into 5-HT terminals plays a significant role in terminating the action of microiontophoretically applied 5-HT. In a second series of experiments, rats were treated with paroxetine (10 mg/kg/d, s.c.) for 2 or 21 d. In both treatment groups, there was a marked prolongation of the effect of microiontophoretically applied 5-HT; however, in rats treated for 2 d, the prolongation was significantly greater than that observed in rats treated for 21 d. After the 21 d treatment with paroxetine and a 48 hr washout, the prolongation of the effect of microiontophoretically applied 5-HT by acute intravenous paroxetine was significantly reduced, suggesting a decrease in the number of 5-HT carriers. In keeping with this interpretation, following the same treatment regimen, there was a 50% and 60% reduction of the in vitro 3H-5-HT uptake in hippocampal and dorsal raphe slices, respectively, and a reduced effectiveness of paroxetine in blocking 3H-5-HT uptake in both regions. The determination of the binding parameters of 3H-paroxetine revealed that, in rats treated for 21 d with paroxetine (10 mg/kg/d, s.c.), following a 48 hr washout Kd values were unchanged but Bmax values were reduced by 70% and 60% in hippocampal and cortical membranes, respectively.</jats:p> Desensitization of the neuronal 5-HT carrier following its long-term blockade The Journal of Neuroscience |
| doi_str_mv |
10.1523/jneurosci.14-05-03036.1994 |
| facet_avail |
Online Free |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTUyMy9qbmV1cm9zY2kuMTQtMDUtMDMwMzYuMTk5NA |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTUyMy9qbmV1cm9zY2kuMTQtMDUtMDMwMzYuMTk5NA |
| institution |
DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 |
| imprint |
Society for Neuroscience, 1994 |
| imprint_str_mv |
Society for Neuroscience, 1994 |
| issn |
0270-6474 1529-2401 |
| issn_str_mv |
0270-6474 1529-2401 |
| language |
English |
| mega_collection |
Society for Neuroscience (CrossRef) |
| match_str |
pineyro1994desensitizationoftheneuronal5htcarrierfollowingitslongtermblockade |
| publishDateSort |
1994 |
| publisher |
Society for Neuroscience |
| recordtype |
ai |
| record_format |
ai |
| series |
The Journal of Neuroscience |
| source_id |
49 |
| title |
Desensitization of the neuronal 5-HT carrier following its long-term blockade |
| title_unstemmed |
Desensitization of the neuronal 5-HT carrier following its long-term blockade |
| title_full |
Desensitization of the neuronal 5-HT carrier following its long-term blockade |
| title_fullStr |
Desensitization of the neuronal 5-HT carrier following its long-term blockade |
| title_full_unstemmed |
Desensitization of the neuronal 5-HT carrier following its long-term blockade |
| title_short |
Desensitization of the neuronal 5-HT carrier following its long-term blockade |
| title_sort |
desensitization of the neuronal 5-ht carrier following its long-term blockade |
| topic |
General Neuroscience |
| url |
http://dx.doi.org/10.1523/jneurosci.14-05-03036.1994 |
| publishDate |
1994 |
| physical |
3036-3047 |
| description |
<jats:p>In vivo extracellular unitary recordings, in vitro 3H-5-hydroxy- tryptamine (5-HT) uptake, and 3H-paroxetine binding assays were used to assess the effect of acute and long-term administration of the 5-HT reuptake inhibitor paroxetine on the neuronal 5-HT transporter in the rat dorsal hippocampus. Recovery time of the firing activity of CA3 hippocampus pyramidal neurons following microiontophoretic application of 5-HT was used as an index of in vivo reuptake activity. In a first series of experiments, the acute intravenous administration of paroxetine and 5-HT denervation with the neurotoxin 5,7- dihydroxytryptamine produced a marked prolongation of the suppressant effect of 5-HT, indicating that reuptake into 5-HT terminals plays a significant role in terminating the action of microiontophoretically applied 5-HT. In a second series of experiments, rats were treated with paroxetine (10 mg/kg/d, s.c.) for 2 or 21 d. In both treatment groups, there was a marked prolongation of the effect of microiontophoretically applied 5-HT; however, in rats treated for 2 d, the prolongation was significantly greater than that observed in rats treated for 21 d. After the 21 d treatment with paroxetine and a 48 hr washout, the prolongation of the effect of microiontophoretically applied 5-HT by acute intravenous paroxetine was significantly reduced, suggesting a decrease in the number of 5-HT carriers. In keeping with this interpretation, following the same treatment regimen, there was a 50% and 60% reduction of the in vitro 3H-5-HT uptake in hippocampal and dorsal raphe slices, respectively, and a reduced effectiveness of paroxetine in blocking 3H-5-HT uptake in both regions. The determination of the binding parameters of 3H-paroxetine revealed that, in rats treated for 21 d with paroxetine (10 mg/kg/d, s.c.), following a 48 hr washout Kd values were unchanged but Bmax values were reduced by 70% and 60% in hippocampal and cortical membranes, respectively.</jats:p> |
| container_issue |
5 |
| container_start_page |
3036 |
| container_title |
The Journal of Neuroscience |
| container_volume |
14 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792338929897177099 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T15:40:02.644Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Desensitization+of+the+neuronal+5-HT+carrier+following+its+long-term+blockade&rft.date=1994-05-01&genre=article&issn=1529-2401&volume=14&issue=5&spage=3036&epage=3047&pages=3036-3047&jtitle=The+Journal+of+Neuroscience&atitle=Desensitization+of+the+neuronal+5-HT+carrier+following+its+long-term+blockade&aulast=de+Montigny&aufirst=C&rft_id=info%3Adoi%2F10.1523%2Fjneurosci.14-05-03036.1994&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792338929897177099 |
| author | Pineyro, G, Blier, P, Dennis, T, de Montigny, C |
| author_facet | Pineyro, G, Blier, P, Dennis, T, de Montigny, C, Pineyro, G, Blier, P, Dennis, T, de Montigny, C |
| author_sort | pineyro, g |
| container_issue | 5 |
| container_start_page | 3036 |
| container_title | The Journal of Neuroscience |
| container_volume | 14 |
| description | <jats:p>In vivo extracellular unitary recordings, in vitro 3H-5-hydroxy- tryptamine (5-HT) uptake, and 3H-paroxetine binding assays were used to assess the effect of acute and long-term administration of the 5-HT reuptake inhibitor paroxetine on the neuronal 5-HT transporter in the rat dorsal hippocampus. Recovery time of the firing activity of CA3 hippocampus pyramidal neurons following microiontophoretic application of 5-HT was used as an index of in vivo reuptake activity. In a first series of experiments, the acute intravenous administration of paroxetine and 5-HT denervation with the neurotoxin 5,7- dihydroxytryptamine produced a marked prolongation of the suppressant effect of 5-HT, indicating that reuptake into 5-HT terminals plays a significant role in terminating the action of microiontophoretically applied 5-HT. In a second series of experiments, rats were treated with paroxetine (10 mg/kg/d, s.c.) for 2 or 21 d. In both treatment groups, there was a marked prolongation of the effect of microiontophoretically applied 5-HT; however, in rats treated for 2 d, the prolongation was significantly greater than that observed in rats treated for 21 d. After the 21 d treatment with paroxetine and a 48 hr washout, the prolongation of the effect of microiontophoretically applied 5-HT by acute intravenous paroxetine was significantly reduced, suggesting a decrease in the number of 5-HT carriers. In keeping with this interpretation, following the same treatment regimen, there was a 50% and 60% reduction of the in vitro 3H-5-HT uptake in hippocampal and dorsal raphe slices, respectively, and a reduced effectiveness of paroxetine in blocking 3H-5-HT uptake in both regions. The determination of the binding parameters of 3H-paroxetine revealed that, in rats treated for 21 d with paroxetine (10 mg/kg/d, s.c.), following a 48 hr washout Kd values were unchanged but Bmax values were reduced by 70% and 60% in hippocampal and cortical membranes, respectively.</jats:p> |
| doi_str_mv | 10.1523/jneurosci.14-05-03036.1994 |
| facet_avail | Online, Free |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTUyMy9qbmV1cm9zY2kuMTQtMDUtMDMwMzYuMTk5NA |
| imprint | Society for Neuroscience, 1994 |
| imprint_str_mv | Society for Neuroscience, 1994 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
| issn | 0270-6474, 1529-2401 |
| issn_str_mv | 0270-6474, 1529-2401 |
| language | English |
| last_indexed | 2024-03-01T15:40:02.644Z |
| match_str | pineyro1994desensitizationoftheneuronal5htcarrierfollowingitslongtermblockade |
| mega_collection | Society for Neuroscience (CrossRef) |
| physical | 3036-3047 |
| publishDate | 1994 |
| publishDateSort | 1994 |
| publisher | Society for Neuroscience |
| record_format | ai |
| recordtype | ai |
| series | The Journal of Neuroscience |
| source_id | 49 |
| spelling | Pineyro, G Blier, P Dennis, T de Montigny, C 0270-6474 1529-2401 Society for Neuroscience General Neuroscience http://dx.doi.org/10.1523/jneurosci.14-05-03036.1994 <jats:p>In vivo extracellular unitary recordings, in vitro 3H-5-hydroxy- tryptamine (5-HT) uptake, and 3H-paroxetine binding assays were used to assess the effect of acute and long-term administration of the 5-HT reuptake inhibitor paroxetine on the neuronal 5-HT transporter in the rat dorsal hippocampus. Recovery time of the firing activity of CA3 hippocampus pyramidal neurons following microiontophoretic application of 5-HT was used as an index of in vivo reuptake activity. In a first series of experiments, the acute intravenous administration of paroxetine and 5-HT denervation with the neurotoxin 5,7- dihydroxytryptamine produced a marked prolongation of the suppressant effect of 5-HT, indicating that reuptake into 5-HT terminals plays a significant role in terminating the action of microiontophoretically applied 5-HT. In a second series of experiments, rats were treated with paroxetine (10 mg/kg/d, s.c.) for 2 or 21 d. In both treatment groups, there was a marked prolongation of the effect of microiontophoretically applied 5-HT; however, in rats treated for 2 d, the prolongation was significantly greater than that observed in rats treated for 21 d. After the 21 d treatment with paroxetine and a 48 hr washout, the prolongation of the effect of microiontophoretically applied 5-HT by acute intravenous paroxetine was significantly reduced, suggesting a decrease in the number of 5-HT carriers. In keeping with this interpretation, following the same treatment regimen, there was a 50% and 60% reduction of the in vitro 3H-5-HT uptake in hippocampal and dorsal raphe slices, respectively, and a reduced effectiveness of paroxetine in blocking 3H-5-HT uptake in both regions. The determination of the binding parameters of 3H-paroxetine revealed that, in rats treated for 21 d with paroxetine (10 mg/kg/d, s.c.), following a 48 hr washout Kd values were unchanged but Bmax values were reduced by 70% and 60% in hippocampal and cortical membranes, respectively.</jats:p> Desensitization of the neuronal 5-HT carrier following its long-term blockade The Journal of Neuroscience |
| spellingShingle | Pineyro, G, Blier, P, Dennis, T, de Montigny, C, The Journal of Neuroscience, Desensitization of the neuronal 5-HT carrier following its long-term blockade, General Neuroscience |
| title | Desensitization of the neuronal 5-HT carrier following its long-term blockade |
| title_full | Desensitization of the neuronal 5-HT carrier following its long-term blockade |
| title_fullStr | Desensitization of the neuronal 5-HT carrier following its long-term blockade |
| title_full_unstemmed | Desensitization of the neuronal 5-HT carrier following its long-term blockade |
| title_short | Desensitization of the neuronal 5-HT carrier following its long-term blockade |
| title_sort | desensitization of the neuronal 5-ht carrier following its long-term blockade |
| title_unstemmed | Desensitization of the neuronal 5-HT carrier following its long-term blockade |
| topic | General Neuroscience |
| url | http://dx.doi.org/10.1523/jneurosci.14-05-03036.1994 |