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Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant org...
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Zeitschriftentitel: | Brazilian Journal of Plant Physiology |
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Personen und Körperschaften: | , , , |
In: | Brazilian Journal of Plant Physiology, 15, 2003, 1, S. 55-58 |
Format: | E-Article |
Sprache: | Unbestimmt |
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author_facet |
Gaborit, Charlotte Caillet, Victoria Deshayes, Alain Marraccini, Pierre Gaborit, Charlotte Caillet, Victoria Deshayes, Alain Marraccini, Pierre |
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author |
Gaborit, Charlotte Caillet, Victoria Deshayes, Alain Marraccini, Pierre |
spellingShingle |
Gaborit, Charlotte Caillet, Victoria Deshayes, Alain Marraccini, Pierre Brazilian Journal of Plant Physiology Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs Plant Science Agronomy and Crop Science |
author_sort |
gaborit, charlotte |
spelling |
Gaborit, Charlotte Caillet, Victoria Deshayes, Alain Marraccini, Pierre 1677-0420 FapUNIFESP (SciELO) Plant Science Agronomy and Crop Science http://dx.doi.org/10.1590/s1677-04202003000100008 <jats:p>A full-length cDNA (CaSUI1) was isolated from a Coffea arabica cDNA library from beans during maturation. Its putative translational product is highly homologous to the SUI1 protein of Saccharomyces cerevisiae which functions in concert with eIF2 and the initiator tRNA-Methionin in directing the ribosome to the proper start site of translation. The corresponding gene from coffee was also cloned and sequenced. Its organization is very similar to what observed for the same gene from rice (Oryza sativa) particularly regarding the position and length of its introns. The expression of CaSUI1 gene was also checked and showed that it was highly expressed in all coffee tissues analyzed, therefore confirming the essential role of the SUI1 protein in cell housekeeping functions.</jats:p> Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs Brazilian Journal of Plant Physiology |
doi_str_mv |
10.1590/s1677-04202003000100008 |
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Biologie Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft |
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Brazilian Journal of Plant Physiology |
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title |
Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs |
title_unstemmed |
Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs |
title_full |
Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs |
title_fullStr |
Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs |
title_full_unstemmed |
Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs |
title_short |
Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs |
title_sort |
molecular cloning of a full-length cdna and gene from coffea arabica encoding a protein homologous to the yeast translation initiation factor sui1: expression analysis in plant organs |
topic |
Plant Science Agronomy and Crop Science |
url |
http://dx.doi.org/10.1590/s1677-04202003000100008 |
publishDate |
2003 |
physical |
55-58 |
description |
<jats:p>A full-length cDNA (CaSUI1) was isolated from a Coffea arabica cDNA library from beans during maturation. Its putative translational product is highly homologous to the SUI1 protein of Saccharomyces cerevisiae which functions in concert with eIF2 and the initiator tRNA-Methionin in directing the ribosome to the proper start site of translation. The corresponding gene from coffee was also cloned and sequenced. Its organization is very similar to what observed for the same gene from rice (Oryza sativa) particularly regarding the position and length of its introns. The expression of CaSUI1 gene was also checked and showed that it was highly expressed in all coffee tissues analyzed, therefore confirming the essential role of the SUI1 protein in cell housekeeping functions.</jats:p> |
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author | Gaborit, Charlotte, Caillet, Victoria, Deshayes, Alain, Marraccini, Pierre |
author_facet | Gaborit, Charlotte, Caillet, Victoria, Deshayes, Alain, Marraccini, Pierre, Gaborit, Charlotte, Caillet, Victoria, Deshayes, Alain, Marraccini, Pierre |
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container_start_page | 55 |
container_title | Brazilian Journal of Plant Physiology |
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description | <jats:p>A full-length cDNA (CaSUI1) was isolated from a Coffea arabica cDNA library from beans during maturation. Its putative translational product is highly homologous to the SUI1 protein of Saccharomyces cerevisiae which functions in concert with eIF2 and the initiator tRNA-Methionin in directing the ribosome to the proper start site of translation. The corresponding gene from coffee was also cloned and sequenced. Its organization is very similar to what observed for the same gene from rice (Oryza sativa) particularly regarding the position and length of its introns. The expression of CaSUI1 gene was also checked and showed that it was highly expressed in all coffee tissues analyzed, therefore confirming the essential role of the SUI1 protein in cell housekeeping functions.</jats:p> |
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spelling | Gaborit, Charlotte Caillet, Victoria Deshayes, Alain Marraccini, Pierre 1677-0420 FapUNIFESP (SciELO) Plant Science Agronomy and Crop Science http://dx.doi.org/10.1590/s1677-04202003000100008 <jats:p>A full-length cDNA (CaSUI1) was isolated from a Coffea arabica cDNA library from beans during maturation. Its putative translational product is highly homologous to the SUI1 protein of Saccharomyces cerevisiae which functions in concert with eIF2 and the initiator tRNA-Methionin in directing the ribosome to the proper start site of translation. The corresponding gene from coffee was also cloned and sequenced. Its organization is very similar to what observed for the same gene from rice (Oryza sativa) particularly regarding the position and length of its introns. The expression of CaSUI1 gene was also checked and showed that it was highly expressed in all coffee tissues analyzed, therefore confirming the essential role of the SUI1 protein in cell housekeeping functions.</jats:p> Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs Brazilian Journal of Plant Physiology |
spellingShingle | Gaborit, Charlotte, Caillet, Victoria, Deshayes, Alain, Marraccini, Pierre, Brazilian Journal of Plant Physiology, Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs, Plant Science, Agronomy and Crop Science |
title | Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs |
title_full | Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs |
title_fullStr | Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs |
title_full_unstemmed | Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs |
title_short | Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs |
title_sort | molecular cloning of a full-length cdna and gene from coffea arabica encoding a protein homologous to the yeast translation initiation factor sui1: expression analysis in plant organs |
title_unstemmed | Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs |
topic | Plant Science, Agronomy and Crop Science |
url | http://dx.doi.org/10.1590/s1677-04202003000100008 |