author_facet Gaborit, Charlotte
Caillet, Victoria
Deshayes, Alain
Marraccini, Pierre
Gaborit, Charlotte
Caillet, Victoria
Deshayes, Alain
Marraccini, Pierre
author Gaborit, Charlotte
Caillet, Victoria
Deshayes, Alain
Marraccini, Pierre
spellingShingle Gaborit, Charlotte
Caillet, Victoria
Deshayes, Alain
Marraccini, Pierre
Brazilian Journal of Plant Physiology
Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs
Plant Science
Agronomy and Crop Science
author_sort gaborit, charlotte
spelling Gaborit, Charlotte Caillet, Victoria Deshayes, Alain Marraccini, Pierre 1677-0420 FapUNIFESP (SciELO) Plant Science Agronomy and Crop Science http://dx.doi.org/10.1590/s1677-04202003000100008 <jats:p>A full-length cDNA (CaSUI1) was isolated from a Coffea arabica cDNA library from beans during maturation. Its putative translational product is highly homologous to the SUI1 protein of Saccharomyces cerevisiae which functions in concert with eIF2 and the initiator tRNA-Methionin in directing the ribosome to the proper start site of translation. The corresponding gene from coffee was also cloned and sequenced. Its organization is very similar to what observed for the same gene from rice (Oryza sativa) particularly regarding the position and length of its introns. The expression of CaSUI1 gene was also checked and showed that it was highly expressed in all coffee tissues analyzed, therefore confirming the essential role of the SUI1 protein in cell housekeeping functions.</jats:p> Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs Brazilian Journal of Plant Physiology
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title Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs
title_unstemmed Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs
title_full Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs
title_fullStr Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs
title_full_unstemmed Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs
title_short Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs
title_sort molecular cloning of a full-length cdna and gene from coffea arabica encoding a protein homologous to the yeast translation initiation factor sui1: expression analysis in plant organs
topic Plant Science
Agronomy and Crop Science
url http://dx.doi.org/10.1590/s1677-04202003000100008
publishDate 2003
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description <jats:p>A full-length cDNA (CaSUI1) was isolated from a Coffea arabica cDNA library from beans during maturation. Its putative translational product is highly homologous to the SUI1 protein of Saccharomyces cerevisiae which functions in concert with eIF2 and the initiator tRNA-Methionin in directing the ribosome to the proper start site of translation. The corresponding gene from coffee was also cloned and sequenced. Its organization is very similar to what observed for the same gene from rice (Oryza sativa) particularly regarding the position and length of its introns. The expression of CaSUI1 gene was also checked and showed that it was highly expressed in all coffee tissues analyzed, therefore confirming the essential role of the SUI1 protein in cell housekeeping functions.</jats:p>
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author Gaborit, Charlotte, Caillet, Victoria, Deshayes, Alain, Marraccini, Pierre
author_facet Gaborit, Charlotte, Caillet, Victoria, Deshayes, Alain, Marraccini, Pierre, Gaborit, Charlotte, Caillet, Victoria, Deshayes, Alain, Marraccini, Pierre
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description <jats:p>A full-length cDNA (CaSUI1) was isolated from a Coffea arabica cDNA library from beans during maturation. Its putative translational product is highly homologous to the SUI1 protein of Saccharomyces cerevisiae which functions in concert with eIF2 and the initiator tRNA-Methionin in directing the ribosome to the proper start site of translation. The corresponding gene from coffee was also cloned and sequenced. Its organization is very similar to what observed for the same gene from rice (Oryza sativa) particularly regarding the position and length of its introns. The expression of CaSUI1 gene was also checked and showed that it was highly expressed in all coffee tissues analyzed, therefore confirming the essential role of the SUI1 protein in cell housekeeping functions.</jats:p>
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spelling Gaborit, Charlotte Caillet, Victoria Deshayes, Alain Marraccini, Pierre 1677-0420 FapUNIFESP (SciELO) Plant Science Agronomy and Crop Science http://dx.doi.org/10.1590/s1677-04202003000100008 <jats:p>A full-length cDNA (CaSUI1) was isolated from a Coffea arabica cDNA library from beans during maturation. Its putative translational product is highly homologous to the SUI1 protein of Saccharomyces cerevisiae which functions in concert with eIF2 and the initiator tRNA-Methionin in directing the ribosome to the proper start site of translation. The corresponding gene from coffee was also cloned and sequenced. Its organization is very similar to what observed for the same gene from rice (Oryza sativa) particularly regarding the position and length of its introns. The expression of CaSUI1 gene was also checked and showed that it was highly expressed in all coffee tissues analyzed, therefore confirming the essential role of the SUI1 protein in cell housekeeping functions.</jats:p> Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs Brazilian Journal of Plant Physiology
spellingShingle Gaborit, Charlotte, Caillet, Victoria, Deshayes, Alain, Marraccini, Pierre, Brazilian Journal of Plant Physiology, Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs, Plant Science, Agronomy and Crop Science
title Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs
title_full Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs
title_fullStr Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs
title_full_unstemmed Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs
title_short Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs
title_sort molecular cloning of a full-length cdna and gene from coffea arabica encoding a protein homologous to the yeast translation initiation factor sui1: expression analysis in plant organs
title_unstemmed Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs
topic Plant Science, Agronomy and Crop Science
url http://dx.doi.org/10.1590/s1677-04202003000100008