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Do blood components affect the production of reactive oxygen species (ROS) by equine synovial cells in vitro?
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| Zeitschriftentitel: | Pesquisa Veterinária Brasileira |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Pesquisa Veterinária Brasileira, 32, 2012, 12, S. 1355-1360 |
| Format: | E-Article |
| Sprache: | Unbestimmt |
| veröffentlicht: |
FapUNIFESP (SciELO)
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| Schlagwörter: |
| author_facet |
Brossi, Patrícia M. Baccarin, Raquel Y.A. Massoco, Cristina O. Brossi, Patrícia M. Baccarin, Raquel Y.A. Massoco, Cristina O. |
|---|---|
| author |
Brossi, Patrícia M. Baccarin, Raquel Y.A. Massoco, Cristina O. |
| spellingShingle |
Brossi, Patrícia M. Baccarin, Raquel Y.A. Massoco, Cristina O. Pesquisa Veterinária Brasileira Do blood components affect the production of reactive oxygen species (ROS) by equine synovial cells in vitro? General Veterinary |
| author_sort |
brossi, patrícia m. |
| spelling |
Brossi, Patrícia M. Baccarin, Raquel Y.A. Massoco, Cristina O. 0100-736X FapUNIFESP (SciELO) General Veterinary http://dx.doi.org/10.1590/s0100-736x2012001200023 <jats:p>Blood-derived products are commonly administered to horses and humans to treat many musculoskeletal diseases, due to their potential antioxidant and anti-inflammatory effects. Nevertheless, antioxidant effects have never been shown upon horse synovial fluid cells in vitro. If proved, this could give a new perspective to justify the clinical application of blood-derived products. The aim of the present study was to investigate the antioxidant effects of two blood-derived products - plasma (unconditioned blood product - UBP) and a commercial blood preparation (conditioned blood product - CBP)¹ - upon stimulated equine synovial fluid cells. Healthy tarsocrural joints (60) were tapped to obtain synovial fluid cells; these cells were pooled, processed, stimulated with lipopolysaccharide (LPS) or phorbol 12-myristate 13-acetate (PMA), and evaluated by flow cytometry for the production of reactive oxygen species (ROS). Upon addition of any blood-derived product here used - UBP and CBP - there was a significant decrease in the oxidative burst of synovial fluid cells (P<0.05). There was no difference between UBP and CBP effects. In conclusion, treatment of stimulated equine synovial cells with either UBP or CBP efficiently restored their redox equilibrium.</jats:p> Do blood components affect the production of reactive oxygen species (ROS) by equine synovial cells in vitro? Pesquisa Veterinária Brasileira |
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| title |
Do blood components affect the production of reactive oxygen species (ROS) by equine synovial cells in vitro? |
| title_unstemmed |
Do blood components affect the production of reactive oxygen species (ROS) by equine synovial cells in vitro? |
| title_full |
Do blood components affect the production of reactive oxygen species (ROS) by equine synovial cells in vitro? |
| title_fullStr |
Do blood components affect the production of reactive oxygen species (ROS) by equine synovial cells in vitro? |
| title_full_unstemmed |
Do blood components affect the production of reactive oxygen species (ROS) by equine synovial cells in vitro? |
| title_short |
Do blood components affect the production of reactive oxygen species (ROS) by equine synovial cells in vitro? |
| title_sort |
do blood components affect the production of reactive oxygen species (ros) by equine synovial cells in vitro? |
| topic |
General Veterinary |
| url |
http://dx.doi.org/10.1590/s0100-736x2012001200023 |
| publishDate |
2012 |
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1355-1360 |
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<jats:p>Blood-derived products are commonly administered to horses and humans to treat many musculoskeletal diseases, due to their potential antioxidant and anti-inflammatory effects. Nevertheless, antioxidant effects have never been shown upon horse synovial fluid cells in vitro. If proved, this could give a new perspective to justify the clinical application of blood-derived products. The aim of the present study was to investigate the antioxidant effects of two blood-derived products - plasma (unconditioned blood product - UBP) and a commercial blood preparation (conditioned blood product - CBP)¹ - upon stimulated equine synovial fluid cells. Healthy tarsocrural joints (60) were tapped to obtain synovial fluid cells; these cells were pooled, processed, stimulated with lipopolysaccharide (LPS) or phorbol 12-myristate 13-acetate (PMA), and evaluated by flow cytometry for the production of reactive oxygen species (ROS). Upon addition of any blood-derived product here used - UBP and CBP - there was a significant decrease in the oxidative burst of synovial fluid cells (P<0.05). There was no difference between UBP and CBP effects. In conclusion, treatment of stimulated equine synovial cells with either UBP or CBP efficiently restored their redox equilibrium.</jats:p> |
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| author | Brossi, Patrícia M., Baccarin, Raquel Y.A., Massoco, Cristina O. |
| author_facet | Brossi, Patrícia M., Baccarin, Raquel Y.A., Massoco, Cristina O., Brossi, Patrícia M., Baccarin, Raquel Y.A., Massoco, Cristina O. |
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| description | <jats:p>Blood-derived products are commonly administered to horses and humans to treat many musculoskeletal diseases, due to their potential antioxidant and anti-inflammatory effects. Nevertheless, antioxidant effects have never been shown upon horse synovial fluid cells in vitro. If proved, this could give a new perspective to justify the clinical application of blood-derived products. The aim of the present study was to investigate the antioxidant effects of two blood-derived products - plasma (unconditioned blood product - UBP) and a commercial blood preparation (conditioned blood product - CBP)¹ - upon stimulated equine synovial fluid cells. Healthy tarsocrural joints (60) were tapped to obtain synovial fluid cells; these cells were pooled, processed, stimulated with lipopolysaccharide (LPS) or phorbol 12-myristate 13-acetate (PMA), and evaluated by flow cytometry for the production of reactive oxygen species (ROS). Upon addition of any blood-derived product here used - UBP and CBP - there was a significant decrease in the oxidative burst of synovial fluid cells (P<0.05). There was no difference between UBP and CBP effects. In conclusion, treatment of stimulated equine synovial cells with either UBP or CBP efficiently restored their redox equilibrium.</jats:p> |
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| spelling | Brossi, Patrícia M. Baccarin, Raquel Y.A. Massoco, Cristina O. 0100-736X FapUNIFESP (SciELO) General Veterinary http://dx.doi.org/10.1590/s0100-736x2012001200023 <jats:p>Blood-derived products are commonly administered to horses and humans to treat many musculoskeletal diseases, due to their potential antioxidant and anti-inflammatory effects. Nevertheless, antioxidant effects have never been shown upon horse synovial fluid cells in vitro. If proved, this could give a new perspective to justify the clinical application of blood-derived products. The aim of the present study was to investigate the antioxidant effects of two blood-derived products - plasma (unconditioned blood product - UBP) and a commercial blood preparation (conditioned blood product - CBP)¹ - upon stimulated equine synovial fluid cells. Healthy tarsocrural joints (60) were tapped to obtain synovial fluid cells; these cells were pooled, processed, stimulated with lipopolysaccharide (LPS) or phorbol 12-myristate 13-acetate (PMA), and evaluated by flow cytometry for the production of reactive oxygen species (ROS). Upon addition of any blood-derived product here used - UBP and CBP - there was a significant decrease in the oxidative burst of synovial fluid cells (P<0.05). There was no difference between UBP and CBP effects. In conclusion, treatment of stimulated equine synovial cells with either UBP or CBP efficiently restored their redox equilibrium.</jats:p> Do blood components affect the production of reactive oxygen species (ROS) by equine synovial cells in vitro? Pesquisa Veterinária Brasileira |
| spellingShingle | Brossi, Patrícia M., Baccarin, Raquel Y.A., Massoco, Cristina O., Pesquisa Veterinária Brasileira, Do blood components affect the production of reactive oxygen species (ROS) by equine synovial cells in vitro?, General Veterinary |
| title | Do blood components affect the production of reactive oxygen species (ROS) by equine synovial cells in vitro? |
| title_full | Do blood components affect the production of reactive oxygen species (ROS) by equine synovial cells in vitro? |
| title_fullStr | Do blood components affect the production of reactive oxygen species (ROS) by equine synovial cells in vitro? |
| title_full_unstemmed | Do blood components affect the production of reactive oxygen species (ROS) by equine synovial cells in vitro? |
| title_short | Do blood components affect the production of reactive oxygen species (ROS) by equine synovial cells in vitro? |
| title_sort | do blood components affect the production of reactive oxygen species (ros) by equine synovial cells in vitro? |
| title_unstemmed | Do blood components affect the production of reactive oxygen species (ROS) by equine synovial cells in vitro? |
| topic | General Veterinary |
| url | http://dx.doi.org/10.1590/s0100-736x2012001200023 |