Eintrag weiter verarbeiten
Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide
Gespeichert in:
| Zeitschriftentitel: | Brazilian Dental Journal |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Brazilian Dental Journal, 25, 2014, 5, S. 367-371 |
| Format: | E-Article |
| Sprache: | Unbestimmt |
| veröffentlicht: |
FapUNIFESP (SciELO)
|
| Schlagwörter: |
| author_facet |
Vargas, Fernanda da Silveira Soares, Diana Gabriela Basso, Fernanda Gonçalves Hebling, Josimeri Costa, Carlos Alberto de Souza Vargas, Fernanda da Silveira Soares, Diana Gabriela Basso, Fernanda Gonçalves Hebling, Josimeri Costa, Carlos Alberto de Souza |
|---|---|
| author |
Vargas, Fernanda da Silveira Soares, Diana Gabriela Basso, Fernanda Gonçalves Hebling, Josimeri Costa, Carlos Alberto de Souza |
| spellingShingle |
Vargas, Fernanda da Silveira Soares, Diana Gabriela Basso, Fernanda Gonçalves Hebling, Josimeri Costa, Carlos Alberto de Souza Brazilian Dental Journal Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide General Dentistry |
| author_sort |
vargas, fernanda da silveira |
| spelling |
Vargas, Fernanda da Silveira Soares, Diana Gabriela Basso, Fernanda Gonçalves Hebling, Josimeri Costa, Carlos Alberto de Souza 0103-6440 FapUNIFESP (SciELO) General Dentistry http://dx.doi.org/10.1590/0103-6440201302434 <jats:p>This in vitro study evaluated the potential protective effect of vitamin E alpha-tocopherol (α-T) isomer against the toxicity of hydrogen peroxide (HP) applied on dental pulp cells. Odontoblast-like MDPC-23 cells were seeded on 96-well plates for 72 h, treated with different concentrations of α-T (1, 3, 5, and 10 mM) for different times (1, 4, 8, and 24 h) and then exposed or not to a 0.018% HP solution for 30 min. In positive and negative control groups, cells were exposed to HP or culture medium (DMEM containing 5% DMSO), respectively. Cell viability was assessed by the MTT assay and the absorbance numeric data, expressed as percentage values, were subjected to the statistical analysis by Kruskal-Wallis and Mann-Whitney tests (α=5%). Considering the cells in the negative control as having 100% of cell viability, all combinations of α-T concentrations and pretreatment times showed a protective effect against HP cytotoxicity. Significant reduction of cell viability (59%) was observed in the positive control compared with the negative control. The highest values of pulp cell viability were obtained after pretreatment with 1 and 3 mM α-T concentrations for 24 h followed by exposure to HP (126% and 97% of cell viability, respectively). Under the tested conditions, the most effective cell protection against the cytotoxic effects of HP was provided by the lowest concentrations of α-T (1 and 3 mM) applied for 24 h.</jats:p> Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide Brazilian Dental Journal |
| doi_str_mv |
10.1590/0103-6440201302434 |
| facet_avail |
Online Free |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTU5MC8wMTAzLTY0NDAyMDEzMDI0MzQ |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTU5MC8wMTAzLTY0NDAyMDEzMDI0MzQ |
| institution |
DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 |
| imprint |
FapUNIFESP (SciELO), 2014 |
| imprint_str_mv |
FapUNIFESP (SciELO), 2014 |
| issn |
0103-6440 |
| issn_str_mv |
0103-6440 |
| language |
Undetermined |
| mega_collection |
FapUNIFESP (SciELO) (CrossRef) |
| match_str |
vargas2014doseresponseandtimecourseofatocoferolmediatingthecytoprotectionofdentalpulpcellsagainsthydrogenperoxide |
| publishDateSort |
2014 |
| publisher |
FapUNIFESP (SciELO) |
| recordtype |
ai |
| record_format |
ai |
| series |
Brazilian Dental Journal |
| source_id |
49 |
| title |
Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide |
| title_unstemmed |
Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide |
| title_full |
Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide |
| title_fullStr |
Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide |
| title_full_unstemmed |
Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide |
| title_short |
Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide |
| title_sort |
dose-response and time-course of a-tocoferol mediating the cytoprotection of dental pulp cells against hydrogen peroxide |
| topic |
General Dentistry |
| url |
http://dx.doi.org/10.1590/0103-6440201302434 |
| publishDate |
2014 |
| physical |
367-371 |
| description |
<jats:p>This in vitro study evaluated the potential protective effect of vitamin E alpha-tocopherol (α-T) isomer against the toxicity of hydrogen peroxide (HP) applied on dental pulp cells. Odontoblast-like MDPC-23 cells were seeded on 96-well plates for 72 h, treated with different concentrations of α-T (1, 3, 5, and 10 mM) for different times (1, 4, 8, and 24 h) and then exposed or not to a 0.018% HP solution for 30 min. In positive and negative control groups, cells were exposed to HP or culture medium (DMEM containing 5% DMSO), respectively. Cell viability was assessed by the MTT assay and the absorbance numeric data, expressed as percentage values, were subjected to the statistical analysis by Kruskal-Wallis and Mann-Whitney tests (α=5%). Considering the cells in the negative control as having 100% of cell viability, all combinations of α-T concentrations and pretreatment times showed a protective effect against HP cytotoxicity. Significant reduction of cell viability (59%) was observed in the positive control compared with the negative control. The highest values of pulp cell viability were obtained after pretreatment with 1 and 3 mM α-T concentrations for 24 h followed by exposure to HP (126% and 97% of cell viability, respectively). Under the tested conditions, the most effective cell protection against the cytotoxic effects of HP was provided by the lowest concentrations of α-T (1 and 3 mM) applied for 24 h.</jats:p> |
| container_issue |
5 |
| container_start_page |
367 |
| container_title |
Brazilian Dental Journal |
| container_volume |
25 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792339710017798149 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T15:51:56.572Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Dose-Response+and+Time-Course+of+a-Tocoferol+Mediating+the+Cytoprotection+Of+Dental+Pulp+Cells+Against+Hydrogen+Peroxide&rft.date=2014-10-01&genre=article&issn=0103-6440&volume=25&issue=5&spage=367&epage=371&pages=367-371&jtitle=Brazilian+Dental+Journal&atitle=Dose-Response+and+Time-Course+of+a-Tocoferol+Mediating+the+Cytoprotection+Of+Dental+Pulp+Cells+Against+Hydrogen+Peroxide&aulast=Costa&aufirst=Carlos+Alberto+de+Souza&rft_id=info%3Adoi%2F10.1590%2F0103-6440201302434&rft.language%5B0%5D=und |
| SOLR | |
| _version_ | 1792339710017798149 |
| author | Vargas, Fernanda da Silveira, Soares, Diana Gabriela, Basso, Fernanda Gonçalves, Hebling, Josimeri, Costa, Carlos Alberto de Souza |
| author_facet | Vargas, Fernanda da Silveira, Soares, Diana Gabriela, Basso, Fernanda Gonçalves, Hebling, Josimeri, Costa, Carlos Alberto de Souza, Vargas, Fernanda da Silveira, Soares, Diana Gabriela, Basso, Fernanda Gonçalves, Hebling, Josimeri, Costa, Carlos Alberto de Souza |
| author_sort | vargas, fernanda da silveira |
| container_issue | 5 |
| container_start_page | 367 |
| container_title | Brazilian Dental Journal |
| container_volume | 25 |
| description | <jats:p>This in vitro study evaluated the potential protective effect of vitamin E alpha-tocopherol (α-T) isomer against the toxicity of hydrogen peroxide (HP) applied on dental pulp cells. Odontoblast-like MDPC-23 cells were seeded on 96-well plates for 72 h, treated with different concentrations of α-T (1, 3, 5, and 10 mM) for different times (1, 4, 8, and 24 h) and then exposed or not to a 0.018% HP solution for 30 min. In positive and negative control groups, cells were exposed to HP or culture medium (DMEM containing 5% DMSO), respectively. Cell viability was assessed by the MTT assay and the absorbance numeric data, expressed as percentage values, were subjected to the statistical analysis by Kruskal-Wallis and Mann-Whitney tests (α=5%). Considering the cells in the negative control as having 100% of cell viability, all combinations of α-T concentrations and pretreatment times showed a protective effect against HP cytotoxicity. Significant reduction of cell viability (59%) was observed in the positive control compared with the negative control. The highest values of pulp cell viability were obtained after pretreatment with 1 and 3 mM α-T concentrations for 24 h followed by exposure to HP (126% and 97% of cell viability, respectively). Under the tested conditions, the most effective cell protection against the cytotoxic effects of HP was provided by the lowest concentrations of α-T (1 and 3 mM) applied for 24 h.</jats:p> |
| doi_str_mv | 10.1590/0103-6440201302434 |
| facet_avail | Online, Free |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTU5MC8wMTAzLTY0NDAyMDEzMDI0MzQ |
| imprint | FapUNIFESP (SciELO), 2014 |
| imprint_str_mv | FapUNIFESP (SciELO), 2014 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
| issn | 0103-6440 |
| issn_str_mv | 0103-6440 |
| language | Undetermined |
| last_indexed | 2024-03-01T15:51:56.572Z |
| match_str | vargas2014doseresponseandtimecourseofatocoferolmediatingthecytoprotectionofdentalpulpcellsagainsthydrogenperoxide |
| mega_collection | FapUNIFESP (SciELO) (CrossRef) |
| physical | 367-371 |
| publishDate | 2014 |
| publishDateSort | 2014 |
| publisher | FapUNIFESP (SciELO) |
| record_format | ai |
| recordtype | ai |
| series | Brazilian Dental Journal |
| source_id | 49 |
| spelling | Vargas, Fernanda da Silveira Soares, Diana Gabriela Basso, Fernanda Gonçalves Hebling, Josimeri Costa, Carlos Alberto de Souza 0103-6440 FapUNIFESP (SciELO) General Dentistry http://dx.doi.org/10.1590/0103-6440201302434 <jats:p>This in vitro study evaluated the potential protective effect of vitamin E alpha-tocopherol (α-T) isomer against the toxicity of hydrogen peroxide (HP) applied on dental pulp cells. Odontoblast-like MDPC-23 cells were seeded on 96-well plates for 72 h, treated with different concentrations of α-T (1, 3, 5, and 10 mM) for different times (1, 4, 8, and 24 h) and then exposed or not to a 0.018% HP solution for 30 min. In positive and negative control groups, cells were exposed to HP or culture medium (DMEM containing 5% DMSO), respectively. Cell viability was assessed by the MTT assay and the absorbance numeric data, expressed as percentage values, were subjected to the statistical analysis by Kruskal-Wallis and Mann-Whitney tests (α=5%). Considering the cells in the negative control as having 100% of cell viability, all combinations of α-T concentrations and pretreatment times showed a protective effect against HP cytotoxicity. Significant reduction of cell viability (59%) was observed in the positive control compared with the negative control. The highest values of pulp cell viability were obtained after pretreatment with 1 and 3 mM α-T concentrations for 24 h followed by exposure to HP (126% and 97% of cell viability, respectively). Under the tested conditions, the most effective cell protection against the cytotoxic effects of HP was provided by the lowest concentrations of α-T (1 and 3 mM) applied for 24 h.</jats:p> Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide Brazilian Dental Journal |
| spellingShingle | Vargas, Fernanda da Silveira, Soares, Diana Gabriela, Basso, Fernanda Gonçalves, Hebling, Josimeri, Costa, Carlos Alberto de Souza, Brazilian Dental Journal, Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide, General Dentistry |
| title | Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide |
| title_full | Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide |
| title_fullStr | Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide |
| title_full_unstemmed | Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide |
| title_short | Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide |
| title_sort | dose-response and time-course of a-tocoferol mediating the cytoprotection of dental pulp cells against hydrogen peroxide |
| title_unstemmed | Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide |
| topic | General Dentistry |
| url | http://dx.doi.org/10.1590/0103-6440201302434 |