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Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs
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| Zeitschriftentitel: | RNA |
|---|---|
| Personen und Körperschaften: | , , |
| In: | RNA, 10, 2004, 12, S. 1957-1966 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Cold Spring Harbor Laboratory
|
| Schlagwörter: |
| author_facet |
CAI, XUEZHONG HAGEDORN, CURT H. CULLEN, BRYAN R. CAI, XUEZHONG HAGEDORN, CURT H. CULLEN, BRYAN R. |
|---|---|
| author |
CAI, XUEZHONG HAGEDORN, CURT H. CULLEN, BRYAN R. |
| spellingShingle |
CAI, XUEZHONG HAGEDORN, CURT H. CULLEN, BRYAN R. RNA Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs Molecular Biology |
| author_sort |
cai, xuezhong |
| spelling |
CAI, XUEZHONG HAGEDORN, CURT H. CULLEN, BRYAN R. 1355-8382 1469-9001 Cold Spring Harbor Laboratory Molecular Biology http://dx.doi.org/10.1261/rna.7135204 <jats:p>The factors regulating the expression of microRNAs (miRNAs), a ubiquitous family of ~22-nt noncoding regulatory RNAs, remain undefined. However, it is known that miRNAs are first transcribed as a largely unstructured precursor, termed a primary miRNA (pri-miRNA), which is sequentially processed in the nucleus, to give the ~65-nt pre-miRNA hairpin intermediate, and then in the cytoplasm, to give the mature miRNA. Here we have sought to identify the RNA polymerase responsible for miRNA transcription and to define the structure of a full-length human miRNA. We show that the pri-miRNA precursors for nine human miRNAs are both capped and polyadenylated and report the sequence of the full-length, ~3433-nt pri-miR-21 RNA. This pri-miR-21 gene sequence is flanked 5′ by a promoter element able to transcribe heterologous mRNAs and 3′ by a consensus polyadenylation sequence. Nuclear processing of pri-miRNAs was found to be efficient, thus largely preventing the nuclear export of full-length pri-miRNAs. Nevertheless, an intact miRNA stem–loop precursor located in the 3′ UTR of a protein coding gene only moderately inhibited expression of the linked open reading frame, probably because the 3′ truncated mRNA could still be exported and expressed. Together, these data show that human pri-miRNAs are not only structurally similar to mRNAs but can, in fact, function both as pri-miRNAs and mRNAs.</jats:p> Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs RNA |
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10.1261/rna.7135204 |
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Online Free |
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Biologie |
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Cold Spring Harbor Laboratory, 2004 |
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Cold Spring Harbor Laboratory, 2004 |
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1355-8382 1469-9001 |
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2004 |
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Cold Spring Harbor Laboratory |
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| title |
Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs |
| title_unstemmed |
Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs |
| title_full |
Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs |
| title_fullStr |
Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs |
| title_full_unstemmed |
Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs |
| title_short |
Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs |
| title_sort |
human micrornas are processed from capped, polyadenylated transcripts that can also function as mrnas |
| topic |
Molecular Biology |
| url |
http://dx.doi.org/10.1261/rna.7135204 |
| publishDate |
2004 |
| physical |
1957-1966 |
| description |
<jats:p>The factors regulating the expression of microRNAs (miRNAs), a ubiquitous family of ~22-nt noncoding regulatory RNAs, remain undefined. However, it is known that miRNAs are first transcribed as a largely unstructured precursor, termed a primary miRNA (pri-miRNA), which is sequentially processed in the nucleus, to give the ~65-nt pre-miRNA hairpin intermediate, and then in the cytoplasm, to give the mature miRNA. Here we have sought to identify the RNA polymerase responsible for miRNA transcription and to define the structure of a full-length human miRNA. We show that the pri-miRNA precursors for nine human miRNAs are both capped and polyadenylated and report the sequence of the full-length, ~3433-nt pri-miR-21 RNA. This pri-miR-21 gene sequence is flanked 5′ by a promoter element able to transcribe heterologous mRNAs and 3′ by a consensus polyadenylation sequence. Nuclear processing of pri-miRNAs was found to be efficient, thus largely preventing the nuclear export of full-length pri-miRNAs. Nevertheless, an intact miRNA stem–loop precursor located in the 3′ UTR of a protein coding gene only moderately inhibited expression of the linked open reading frame, probably because the 3′ truncated mRNA could still be exported and expressed. Together, these data show that human pri-miRNAs are not only structurally similar to mRNAs but can, in fact, function both as pri-miRNAs and mRNAs.</jats:p> |
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| author | CAI, XUEZHONG, HAGEDORN, CURT H., CULLEN, BRYAN R. |
| author_facet | CAI, XUEZHONG, HAGEDORN, CURT H., CULLEN, BRYAN R., CAI, XUEZHONG, HAGEDORN, CURT H., CULLEN, BRYAN R. |
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| description | <jats:p>The factors regulating the expression of microRNAs (miRNAs), a ubiquitous family of ~22-nt noncoding regulatory RNAs, remain undefined. However, it is known that miRNAs are first transcribed as a largely unstructured precursor, termed a primary miRNA (pri-miRNA), which is sequentially processed in the nucleus, to give the ~65-nt pre-miRNA hairpin intermediate, and then in the cytoplasm, to give the mature miRNA. Here we have sought to identify the RNA polymerase responsible for miRNA transcription and to define the structure of a full-length human miRNA. We show that the pri-miRNA precursors for nine human miRNAs are both capped and polyadenylated and report the sequence of the full-length, ~3433-nt pri-miR-21 RNA. This pri-miR-21 gene sequence is flanked 5′ by a promoter element able to transcribe heterologous mRNAs and 3′ by a consensus polyadenylation sequence. Nuclear processing of pri-miRNAs was found to be efficient, thus largely preventing the nuclear export of full-length pri-miRNAs. Nevertheless, an intact miRNA stem–loop precursor located in the 3′ UTR of a protein coding gene only moderately inhibited expression of the linked open reading frame, probably because the 3′ truncated mRNA could still be exported and expressed. Together, these data show that human pri-miRNAs are not only structurally similar to mRNAs but can, in fact, function both as pri-miRNAs and mRNAs.</jats:p> |
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| imprint | Cold Spring Harbor Laboratory, 2004 |
| imprint_str_mv | Cold Spring Harbor Laboratory, 2004 |
| institution | DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1 |
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| spelling | CAI, XUEZHONG HAGEDORN, CURT H. CULLEN, BRYAN R. 1355-8382 1469-9001 Cold Spring Harbor Laboratory Molecular Biology http://dx.doi.org/10.1261/rna.7135204 <jats:p>The factors regulating the expression of microRNAs (miRNAs), a ubiquitous family of ~22-nt noncoding regulatory RNAs, remain undefined. However, it is known that miRNAs are first transcribed as a largely unstructured precursor, termed a primary miRNA (pri-miRNA), which is sequentially processed in the nucleus, to give the ~65-nt pre-miRNA hairpin intermediate, and then in the cytoplasm, to give the mature miRNA. Here we have sought to identify the RNA polymerase responsible for miRNA transcription and to define the structure of a full-length human miRNA. We show that the pri-miRNA precursors for nine human miRNAs are both capped and polyadenylated and report the sequence of the full-length, ~3433-nt pri-miR-21 RNA. This pri-miR-21 gene sequence is flanked 5′ by a promoter element able to transcribe heterologous mRNAs and 3′ by a consensus polyadenylation sequence. Nuclear processing of pri-miRNAs was found to be efficient, thus largely preventing the nuclear export of full-length pri-miRNAs. Nevertheless, an intact miRNA stem–loop precursor located in the 3′ UTR of a protein coding gene only moderately inhibited expression of the linked open reading frame, probably because the 3′ truncated mRNA could still be exported and expressed. Together, these data show that human pri-miRNAs are not only structurally similar to mRNAs but can, in fact, function both as pri-miRNAs and mRNAs.</jats:p> Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs RNA |
| spellingShingle | CAI, XUEZHONG, HAGEDORN, CURT H., CULLEN, BRYAN R., RNA, Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs, Molecular Biology |
| title | Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs |
| title_full | Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs |
| title_fullStr | Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs |
| title_full_unstemmed | Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs |
| title_short | Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs |
| title_sort | human micrornas are processed from capped, polyadenylated transcripts that can also function as mrnas |
| title_unstemmed | Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs |
| topic | Molecular Biology |
| url | http://dx.doi.org/10.1261/rna.7135204 |