Eintrag weiter verarbeiten
Mitochondrial Activity in Rainbow Trout Red Muscle: the Effect of Temperature on the Adp-Dependence of Atp Synthesis
Gespeichert in:
| Zeitschriftentitel: | Journal of Experimental Biology |
|---|---|
| Personen und Körperschaften: | , |
| In: | Journal of Experimental Biology, 176, 1993, 1, S. 145-158 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
The Company of Biologists
|
| Schlagwörter: |
| author_facet |
Blier, Pierre U. Guderley, Helga E. Blier, Pierre U. Guderley, Helga E. |
|---|---|
| author |
Blier, Pierre U. Guderley, Helga E. |
| spellingShingle |
Blier, Pierre U. Guderley, Helga E. Journal of Experimental Biology Mitochondrial Activity in Rainbow Trout Red Muscle: the Effect of Temperature on the Adp-Dependence of Atp Synthesis Insect Science Molecular Biology Animal Science and Zoology Aquatic Science Physiology Ecology, Evolution, Behavior and Systematics |
| author_sort |
blier, pierre u. |
| spelling |
Blier, Pierre U. Guderley, Helga E. 0022-0949 1477-9145 The Company of Biologists Insect Science Molecular Biology Animal Science and Zoology Aquatic Science Physiology Ecology, Evolution, Behavior and Systematics http://dx.doi.org/10.1242/jeb.176.1.145 <jats:title>ABSTRACT</jats:title><jats:p>By coupling mitochondrial ATP production to an enzymatic NADPH-producing reaction, we monitored the rates of ATP production spectrophotometrically. ATP production was coupled to the oxidation of malate and pyruvate. Interference by adenylate kinase was eliminated by using P1,P5-di(adenosine-5′)pentaphosphate and we found no interference by NADPH oxidase. We studied the kinetics of ATP synthesis by mitochondria from rainbow trout red muscle at three temperatures (8, 15 and 22°C) and under two pH regimes (a constant pH of 7.4 and temperature-dependent pH). The mitochondria oxidized pyruvate and malate. The apparent Michaelis constant (Km,app) for ADP as well as the maximal velocity (Vmax) for ADP phosphorylation are markedly affected by temperature but not by pH. The Km,app for ADP decreases with increasing temperature while the Vmax increases. These data suggest that reduced temperatures decrease mitochondrial sensitivity to control by ADP availability.</jats:p> Mitochondrial Activity in Rainbow Trout Red Muscle: the Effect of Temperature on the Adp-Dependence of Atp Synthesis Journal of Experimental Biology |
| doi_str_mv |
10.1242/jeb.176.1.145 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie Geographie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTI0Mi9qZWIuMTc2LjEuMTQ1 |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTI0Mi9qZWIuMTc2LjEuMTQ1 |
| institution |
DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 |
| imprint |
The Company of Biologists, 1993 |
| imprint_str_mv |
The Company of Biologists, 1993 |
| issn |
0022-0949 1477-9145 |
| issn_str_mv |
0022-0949 1477-9145 |
| language |
English |
| mega_collection |
The Company of Biologists (CrossRef) |
| match_str |
blier1993mitochondrialactivityinrainbowtroutredmuscletheeffectoftemperatureontheadpdependenceofatpsynthesis |
| publishDateSort |
1993 |
| publisher |
The Company of Biologists |
| recordtype |
ai |
| record_format |
ai |
| series |
Journal of Experimental Biology |
| source_id |
49 |
| title |
Mitochondrial Activity in Rainbow Trout Red Muscle: the Effect of Temperature on the Adp-Dependence of Atp Synthesis |
| title_unstemmed |
Mitochondrial Activity in Rainbow Trout Red Muscle: the Effect of Temperature on the Adp-Dependence of Atp Synthesis |
| title_full |
Mitochondrial Activity in Rainbow Trout Red Muscle: the Effect of Temperature on the Adp-Dependence of Atp Synthesis |
| title_fullStr |
Mitochondrial Activity in Rainbow Trout Red Muscle: the Effect of Temperature on the Adp-Dependence of Atp Synthesis |
| title_full_unstemmed |
Mitochondrial Activity in Rainbow Trout Red Muscle: the Effect of Temperature on the Adp-Dependence of Atp Synthesis |
| title_short |
Mitochondrial Activity in Rainbow Trout Red Muscle: the Effect of Temperature on the Adp-Dependence of Atp Synthesis |
| title_sort |
mitochondrial activity in rainbow trout red muscle: the effect of temperature on the adp-dependence of atp synthesis |
| topic |
Insect Science Molecular Biology Animal Science and Zoology Aquatic Science Physiology Ecology, Evolution, Behavior and Systematics |
| url |
http://dx.doi.org/10.1242/jeb.176.1.145 |
| publishDate |
1993 |
| physical |
145-158 |
| description |
<jats:title>ABSTRACT</jats:title><jats:p>By coupling mitochondrial ATP production to an enzymatic NADPH-producing reaction, we monitored the rates of ATP production spectrophotometrically. ATP production was coupled to the oxidation of malate and pyruvate. Interference by adenylate kinase was eliminated by using P1,P5-di(adenosine-5′)pentaphosphate and we found no interference by NADPH oxidase. We studied the kinetics of ATP synthesis by mitochondria from rainbow trout red muscle at three temperatures (8, 15 and 22°C) and under two pH regimes (a constant pH of 7.4 and temperature-dependent pH). The mitochondria oxidized pyruvate and malate. The apparent Michaelis constant (Km,app) for ADP as well as the maximal velocity (Vmax) for ADP phosphorylation are markedly affected by temperature but not by pH. The Km,app for ADP decreases with increasing temperature while the Vmax increases. These data suggest that reduced temperatures decrease mitochondrial sensitivity to control by ADP availability.</jats:p> |
| container_issue |
1 |
| container_start_page |
145 |
| container_title |
Journal of Experimental Biology |
| container_volume |
176 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792339145822044162 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T15:43:28.937Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Mitochondrial+Activity+in+Rainbow+Trout+Red+Muscle%3A+the+Effect+of+Temperature+on+the+Adp-Dependence+of+Atp+Synthesis&rft.date=1993-03-01&genre=article&issn=1477-9145&volume=176&issue=1&spage=145&epage=158&pages=145-158&jtitle=Journal+of+Experimental+Biology&atitle=Mitochondrial+Activity+in+Rainbow+Trout+Red+Muscle%3A+the+Effect+of+Temperature+on+the+Adp-Dependence+of+Atp+Synthesis&aulast=Guderley&aufirst=Helga+E.&rft_id=info%3Adoi%2F10.1242%2Fjeb.176.1.145&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792339145822044162 |
| author | Blier, Pierre U., Guderley, Helga E. |
| author_facet | Blier, Pierre U., Guderley, Helga E., Blier, Pierre U., Guderley, Helga E. |
| author_sort | blier, pierre u. |
| container_issue | 1 |
| container_start_page | 145 |
| container_title | Journal of Experimental Biology |
| container_volume | 176 |
| description | <jats:title>ABSTRACT</jats:title><jats:p>By coupling mitochondrial ATP production to an enzymatic NADPH-producing reaction, we monitored the rates of ATP production spectrophotometrically. ATP production was coupled to the oxidation of malate and pyruvate. Interference by adenylate kinase was eliminated by using P1,P5-di(adenosine-5′)pentaphosphate and we found no interference by NADPH oxidase. We studied the kinetics of ATP synthesis by mitochondria from rainbow trout red muscle at three temperatures (8, 15 and 22°C) and under two pH regimes (a constant pH of 7.4 and temperature-dependent pH). The mitochondria oxidized pyruvate and malate. The apparent Michaelis constant (Km,app) for ADP as well as the maximal velocity (Vmax) for ADP phosphorylation are markedly affected by temperature but not by pH. The Km,app for ADP decreases with increasing temperature while the Vmax increases. These data suggest that reduced temperatures decrease mitochondrial sensitivity to control by ADP availability.</jats:p> |
| doi_str_mv | 10.1242/jeb.176.1.145 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie, Geographie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTI0Mi9qZWIuMTc2LjEuMTQ1 |
| imprint | The Company of Biologists, 1993 |
| imprint_str_mv | The Company of Biologists, 1993 |
| institution | DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15 |
| issn | 0022-0949, 1477-9145 |
| issn_str_mv | 0022-0949, 1477-9145 |
| language | English |
| last_indexed | 2024-03-01T15:43:28.937Z |
| match_str | blier1993mitochondrialactivityinrainbowtroutredmuscletheeffectoftemperatureontheadpdependenceofatpsynthesis |
| mega_collection | The Company of Biologists (CrossRef) |
| physical | 145-158 |
| publishDate | 1993 |
| publishDateSort | 1993 |
| publisher | The Company of Biologists |
| record_format | ai |
| recordtype | ai |
| series | Journal of Experimental Biology |
| source_id | 49 |
| spelling | Blier, Pierre U. Guderley, Helga E. 0022-0949 1477-9145 The Company of Biologists Insect Science Molecular Biology Animal Science and Zoology Aquatic Science Physiology Ecology, Evolution, Behavior and Systematics http://dx.doi.org/10.1242/jeb.176.1.145 <jats:title>ABSTRACT</jats:title><jats:p>By coupling mitochondrial ATP production to an enzymatic NADPH-producing reaction, we monitored the rates of ATP production spectrophotometrically. ATP production was coupled to the oxidation of malate and pyruvate. Interference by adenylate kinase was eliminated by using P1,P5-di(adenosine-5′)pentaphosphate and we found no interference by NADPH oxidase. We studied the kinetics of ATP synthesis by mitochondria from rainbow trout red muscle at three temperatures (8, 15 and 22°C) and under two pH regimes (a constant pH of 7.4 and temperature-dependent pH). The mitochondria oxidized pyruvate and malate. The apparent Michaelis constant (Km,app) for ADP as well as the maximal velocity (Vmax) for ADP phosphorylation are markedly affected by temperature but not by pH. The Km,app for ADP decreases with increasing temperature while the Vmax increases. These data suggest that reduced temperatures decrease mitochondrial sensitivity to control by ADP availability.</jats:p> Mitochondrial Activity in Rainbow Trout Red Muscle: the Effect of Temperature on the Adp-Dependence of Atp Synthesis Journal of Experimental Biology |
| spellingShingle | Blier, Pierre U., Guderley, Helga E., Journal of Experimental Biology, Mitochondrial Activity in Rainbow Trout Red Muscle: the Effect of Temperature on the Adp-Dependence of Atp Synthesis, Insect Science, Molecular Biology, Animal Science and Zoology, Aquatic Science, Physiology, Ecology, Evolution, Behavior and Systematics |
| title | Mitochondrial Activity in Rainbow Trout Red Muscle: the Effect of Temperature on the Adp-Dependence of Atp Synthesis |
| title_full | Mitochondrial Activity in Rainbow Trout Red Muscle: the Effect of Temperature on the Adp-Dependence of Atp Synthesis |
| title_fullStr | Mitochondrial Activity in Rainbow Trout Red Muscle: the Effect of Temperature on the Adp-Dependence of Atp Synthesis |
| title_full_unstemmed | Mitochondrial Activity in Rainbow Trout Red Muscle: the Effect of Temperature on the Adp-Dependence of Atp Synthesis |
| title_short | Mitochondrial Activity in Rainbow Trout Red Muscle: the Effect of Temperature on the Adp-Dependence of Atp Synthesis |
| title_sort | mitochondrial activity in rainbow trout red muscle: the effect of temperature on the adp-dependence of atp synthesis |
| title_unstemmed | Mitochondrial Activity in Rainbow Trout Red Muscle: the Effect of Temperature on the Adp-Dependence of Atp Synthesis |
| topic | Insect Science, Molecular Biology, Animal Science and Zoology, Aquatic Science, Physiology, Ecology, Evolution, Behavior and Systematics |
| url | http://dx.doi.org/10.1242/jeb.176.1.145 |