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Distinct 3’ UTRs regulate the life-cycle specific expression of twoTCTPparalogs inTrypanosoma brucei
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| Zeitschriftentitel: | Journal of Cell Science |
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| Personen und Körperschaften: | , , , |
| In: | Journal of Cell Science, 2018 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
The Company of Biologists
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| Schlagwörter: |
| author_facet |
Jojic, Borka Amodeo, Simona Bregy, Irina Ochsenreiter, Torsten Jojic, Borka Amodeo, Simona Bregy, Irina Ochsenreiter, Torsten |
|---|---|
| author |
Jojic, Borka Amodeo, Simona Bregy, Irina Ochsenreiter, Torsten |
| spellingShingle |
Jojic, Borka Amodeo, Simona Bregy, Irina Ochsenreiter, Torsten Journal of Cell Science Distinct 3’ UTRs regulate the life-cycle specific expression of twoTCTPparalogs inTrypanosoma brucei Cell Biology |
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jojic, borka |
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Jojic, Borka Amodeo, Simona Bregy, Irina Ochsenreiter, Torsten 1477-9137 0021-9533 The Company of Biologists Cell Biology http://dx.doi.org/10.1242/jcs.206417 <jats:p>The translationally controlled tumor protein (TCTP) is highly conserved and ubiquitously expressed in eukaryotes. It is involved in growth and development, cell cycle progression, protection against cellular stresses and apoptosis, indicating the multifunctional role of the protein. Here, for the first time we characterize the expression and function of TCTP in the human and animal pathogen, Trypanosoma brucei. We identified two paralogs (TCTP1 and TCTP2) that are differentially expressed in the life cycle of the parasite. The genes have identical 5' UTRs and almost identical open reading frames. The 3' UTRs differ substantially in sequence and length and are sufficient for the exclusive expression of TCTP1 in procyclic and TCTP2 in bloodstream form parasites. Furthermore, we characterize which parts of the 3' UTR are needed for mRNA stability of TCTP2. RNAi experiments demonstrate that TCTP1 and TCTP2 expression is essential for normal cell growth in procyclic and bloodstream form parasites, respectively. Depletion of TCTP1 in procyclic form cells leads to aberrant cell and mitochondrial organelle morphology as well as enlarged and reduced number of acidocalcisomes.</jats:p> Distinct 3’ UTRs regulate the life-cycle specific expression of two<i>TCTP</i>paralogs in<i>Trypanosoma brucei</i> Journal of Cell Science |
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| title |
Distinct 3’ UTRs regulate the life-cycle specific expression of twoTCTPparalogs inTrypanosoma brucei |
| title_unstemmed |
Distinct 3’ UTRs regulate the life-cycle specific expression of twoTCTPparalogs inTrypanosoma brucei |
| title_full |
Distinct 3’ UTRs regulate the life-cycle specific expression of twoTCTPparalogs inTrypanosoma brucei |
| title_fullStr |
Distinct 3’ UTRs regulate the life-cycle specific expression of twoTCTPparalogs inTrypanosoma brucei |
| title_full_unstemmed |
Distinct 3’ UTRs regulate the life-cycle specific expression of twoTCTPparalogs inTrypanosoma brucei |
| title_short |
Distinct 3’ UTRs regulate the life-cycle specific expression of twoTCTPparalogs inTrypanosoma brucei |
| title_sort |
distinct 3’ utrs regulate the life-cycle specific expression of two<i>tctp</i>paralogs in<i>trypanosoma brucei</i> |
| topic |
Cell Biology |
| url |
http://dx.doi.org/10.1242/jcs.206417 |
| publishDate |
2018 |
| physical |
|
| description |
<jats:p>The translationally controlled tumor protein (TCTP) is highly conserved and ubiquitously expressed in eukaryotes. It is involved in growth and development, cell cycle progression, protection against cellular stresses and apoptosis, indicating the multifunctional role of the protein. Here, for the first time we characterize the expression and function of TCTP in the human and animal pathogen, Trypanosoma brucei. We identified two paralogs (TCTP1 and TCTP2) that are differentially expressed in the life cycle of the parasite. The genes have identical 5' UTRs and almost identical open reading frames. The 3' UTRs differ substantially in sequence and length and are sufficient for the exclusive expression of TCTP1 in procyclic and TCTP2 in bloodstream form parasites. Furthermore, we characterize which parts of the 3' UTR are needed for mRNA stability of TCTP2. RNAi experiments demonstrate that TCTP1 and TCTP2 expression is essential for normal cell growth in procyclic and bloodstream form parasites, respectively. Depletion of TCTP1 in procyclic form cells leads to aberrant cell and mitochondrial organelle morphology as well as enlarged and reduced number of acidocalcisomes.</jats:p> |
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| author | Jojic, Borka, Amodeo, Simona, Bregy, Irina, Ochsenreiter, Torsten |
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| description | <jats:p>The translationally controlled tumor protein (TCTP) is highly conserved and ubiquitously expressed in eukaryotes. It is involved in growth and development, cell cycle progression, protection against cellular stresses and apoptosis, indicating the multifunctional role of the protein. Here, for the first time we characterize the expression and function of TCTP in the human and animal pathogen, Trypanosoma brucei. We identified two paralogs (TCTP1 and TCTP2) that are differentially expressed in the life cycle of the parasite. The genes have identical 5' UTRs and almost identical open reading frames. The 3' UTRs differ substantially in sequence and length and are sufficient for the exclusive expression of TCTP1 in procyclic and TCTP2 in bloodstream form parasites. Furthermore, we characterize which parts of the 3' UTR are needed for mRNA stability of TCTP2. RNAi experiments demonstrate that TCTP1 and TCTP2 expression is essential for normal cell growth in procyclic and bloodstream form parasites, respectively. Depletion of TCTP1 in procyclic form cells leads to aberrant cell and mitochondrial organelle morphology as well as enlarged and reduced number of acidocalcisomes.</jats:p> |
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| spelling | Jojic, Borka Amodeo, Simona Bregy, Irina Ochsenreiter, Torsten 1477-9137 0021-9533 The Company of Biologists Cell Biology http://dx.doi.org/10.1242/jcs.206417 <jats:p>The translationally controlled tumor protein (TCTP) is highly conserved and ubiquitously expressed in eukaryotes. It is involved in growth and development, cell cycle progression, protection against cellular stresses and apoptosis, indicating the multifunctional role of the protein. Here, for the first time we characterize the expression and function of TCTP in the human and animal pathogen, Trypanosoma brucei. We identified two paralogs (TCTP1 and TCTP2) that are differentially expressed in the life cycle of the parasite. The genes have identical 5' UTRs and almost identical open reading frames. The 3' UTRs differ substantially in sequence and length and are sufficient for the exclusive expression of TCTP1 in procyclic and TCTP2 in bloodstream form parasites. Furthermore, we characterize which parts of the 3' UTR are needed for mRNA stability of TCTP2. RNAi experiments demonstrate that TCTP1 and TCTP2 expression is essential for normal cell growth in procyclic and bloodstream form parasites, respectively. Depletion of TCTP1 in procyclic form cells leads to aberrant cell and mitochondrial organelle morphology as well as enlarged and reduced number of acidocalcisomes.</jats:p> Distinct 3’ UTRs regulate the life-cycle specific expression of two<i>TCTP</i>paralogs in<i>Trypanosoma brucei</i> Journal of Cell Science |
| spellingShingle | Jojic, Borka, Amodeo, Simona, Bregy, Irina, Ochsenreiter, Torsten, Journal of Cell Science, Distinct 3’ UTRs regulate the life-cycle specific expression of twoTCTPparalogs inTrypanosoma brucei, Cell Biology |
| title | Distinct 3’ UTRs regulate the life-cycle specific expression of twoTCTPparalogs inTrypanosoma brucei |
| title_full | Distinct 3’ UTRs regulate the life-cycle specific expression of twoTCTPparalogs inTrypanosoma brucei |
| title_fullStr | Distinct 3’ UTRs regulate the life-cycle specific expression of twoTCTPparalogs inTrypanosoma brucei |
| title_full_unstemmed | Distinct 3’ UTRs regulate the life-cycle specific expression of twoTCTPparalogs inTrypanosoma brucei |
| title_short | Distinct 3’ UTRs regulate the life-cycle specific expression of twoTCTPparalogs inTrypanosoma brucei |
| title_sort | distinct 3’ utrs regulate the life-cycle specific expression of two<i>tctp</i>paralogs in<i>trypanosoma brucei</i> |
| title_unstemmed | Distinct 3’ UTRs regulate the life-cycle specific expression of twoTCTPparalogs inTrypanosoma brucei |
| topic | Cell Biology |
| url | http://dx.doi.org/10.1242/jcs.206417 |