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Upregulation of chicken p15INK4b at senescence and in the developing brain
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| Zeitschriftentitel: | Journal of Cell Science |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , |
| In: | Journal of Cell Science, 119, 2006, 12, S. 2435-2443 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
The Company of Biologists
|
| Schlagwörter: |
| author_facet |
Kim, S.-H. Rowe, J. Fujii, H. Jones, R. Schmierer, B. Kong, B.-W. Kuchler, K. Foster, D. Ish-Horowicz, D. Peters, G. Kim, S.-H. Rowe, J. Fujii, H. Jones, R. Schmierer, B. Kong, B.-W. Kuchler, K. Foster, D. Ish-Horowicz, D. Peters, G. |
|---|---|
| author |
Kim, S.-H. Rowe, J. Fujii, H. Jones, R. Schmierer, B. Kong, B.-W. Kuchler, K. Foster, D. Ish-Horowicz, D. Peters, G. |
| spellingShingle |
Kim, S.-H. Rowe, J. Fujii, H. Jones, R. Schmierer, B. Kong, B.-W. Kuchler, K. Foster, D. Ish-Horowicz, D. Peters, G. Journal of Cell Science Upregulation of chicken p15INK4b at senescence and in the developing brain Cell Biology |
| author_sort |
kim, s.-h. |
| spelling |
Kim, S.-H. Rowe, J. Fujii, H. Jones, R. Schmierer, B. Kong, B.-W. Kuchler, K. Foster, D. Ish-Horowicz, D. Peters, G. 1477-9137 0021-9533 The Company of Biologists Cell Biology http://dx.doi.org/10.1242/jcs.02989 <jats:p>In mammalian cells, products of the INK4a-ARF locus play major roles in senescence and tumour suppression in different contexts, whereas the adjacent INK4b gene is more generally associated with transforming growth factor β (TGF-β)-mediated growth arrest. As the chicken genome does not encode an equivalent of INK4a, we asked whether INK4b and/or ARF contribute to replicative senescence in chicken cells. In chicken embryo fibroblasts (CEFs), INK4b levels increase substantially at senescence and the gene is transcriptionally silenced in two spontaneously immortalised chicken cell lines. By contrast, ARF levels are unaffected by prolonged culture or immortalisation. These expression patterns resemble the behaviour of INK4a and ARF in human fibroblasts. However, short-hairpin RNA (shRNA)-mediated knockdown of chicken INK4b or ARF provides only modest lifespan extension, suggesting that other factors contribute to senescence in CEFs. As well as underscoring the importance of the INK4b-ARF-INK4a locus in senescence, these findings imply that the encoded products have assumed different roles in different evolutionary niches. Although ARF RNA is not detectable in early chicken embryos, the INK4b transcript is expressed in the roof-plate of the developing hind-brain, consistent with a role in limiting cell proliferation.</jats:p> Upregulation of chicken p15INK4b at senescence and in the developing brain Journal of Cell Science |
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The Company of Biologists, 2006 |
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Journal of Cell Science |
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| title |
Upregulation of chicken p15INK4b at senescence and in the developing brain |
| title_unstemmed |
Upregulation of chicken p15INK4b at senescence and in the developing brain |
| title_full |
Upregulation of chicken p15INK4b at senescence and in the developing brain |
| title_fullStr |
Upregulation of chicken p15INK4b at senescence and in the developing brain |
| title_full_unstemmed |
Upregulation of chicken p15INK4b at senescence and in the developing brain |
| title_short |
Upregulation of chicken p15INK4b at senescence and in the developing brain |
| title_sort |
upregulation of chicken p15ink4b at senescence and in the developing brain |
| topic |
Cell Biology |
| url |
http://dx.doi.org/10.1242/jcs.02989 |
| publishDate |
2006 |
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2435-2443 |
| description |
<jats:p>In mammalian cells, products of the INK4a-ARF locus play major roles in senescence and tumour suppression in different contexts, whereas the adjacent INK4b gene is more generally associated with transforming growth factor β (TGF-β)-mediated growth arrest. As the chicken genome does not encode an equivalent of INK4a, we asked whether INK4b and/or ARF contribute to replicative senescence in chicken cells. In chicken embryo fibroblasts (CEFs), INK4b levels increase substantially at senescence and the gene is transcriptionally silenced in two spontaneously immortalised chicken cell lines. By contrast, ARF levels are unaffected by prolonged culture or immortalisation. These expression patterns resemble the behaviour of INK4a and ARF in human fibroblasts. However, short-hairpin RNA (shRNA)-mediated knockdown of chicken INK4b or ARF provides only modest lifespan extension, suggesting that other factors contribute to senescence in CEFs. As well as underscoring the importance of the INK4b-ARF-INK4a locus in senescence, these findings imply that the encoded products have assumed different roles in different evolutionary niches. Although ARF RNA is not detectable in early chicken embryos, the INK4b transcript is expressed in the roof-plate of the developing hind-brain, consistent with a role in limiting cell proliferation.</jats:p> |
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| author | Kim, S.-H., Rowe, J., Fujii, H., Jones, R., Schmierer, B., Kong, B.-W., Kuchler, K., Foster, D., Ish-Horowicz, D., Peters, G. |
| author_facet | Kim, S.-H., Rowe, J., Fujii, H., Jones, R., Schmierer, B., Kong, B.-W., Kuchler, K., Foster, D., Ish-Horowicz, D., Peters, G., Kim, S.-H., Rowe, J., Fujii, H., Jones, R., Schmierer, B., Kong, B.-W., Kuchler, K., Foster, D., Ish-Horowicz, D., Peters, G. |
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| description | <jats:p>In mammalian cells, products of the INK4a-ARF locus play major roles in senescence and tumour suppression in different contexts, whereas the adjacent INK4b gene is more generally associated with transforming growth factor β (TGF-β)-mediated growth arrest. As the chicken genome does not encode an equivalent of INK4a, we asked whether INK4b and/or ARF contribute to replicative senescence in chicken cells. In chicken embryo fibroblasts (CEFs), INK4b levels increase substantially at senescence and the gene is transcriptionally silenced in two spontaneously immortalised chicken cell lines. By contrast, ARF levels are unaffected by prolonged culture or immortalisation. These expression patterns resemble the behaviour of INK4a and ARF in human fibroblasts. However, short-hairpin RNA (shRNA)-mediated knockdown of chicken INK4b or ARF provides only modest lifespan extension, suggesting that other factors contribute to senescence in CEFs. As well as underscoring the importance of the INK4b-ARF-INK4a locus in senescence, these findings imply that the encoded products have assumed different roles in different evolutionary niches. Although ARF RNA is not detectable in early chicken embryos, the INK4b transcript is expressed in the roof-plate of the developing hind-brain, consistent with a role in limiting cell proliferation.</jats:p> |
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| spelling | Kim, S.-H. Rowe, J. Fujii, H. Jones, R. Schmierer, B. Kong, B.-W. Kuchler, K. Foster, D. Ish-Horowicz, D. Peters, G. 1477-9137 0021-9533 The Company of Biologists Cell Biology http://dx.doi.org/10.1242/jcs.02989 <jats:p>In mammalian cells, products of the INK4a-ARF locus play major roles in senescence and tumour suppression in different contexts, whereas the adjacent INK4b gene is more generally associated with transforming growth factor β (TGF-β)-mediated growth arrest. As the chicken genome does not encode an equivalent of INK4a, we asked whether INK4b and/or ARF contribute to replicative senescence in chicken cells. In chicken embryo fibroblasts (CEFs), INK4b levels increase substantially at senescence and the gene is transcriptionally silenced in two spontaneously immortalised chicken cell lines. By contrast, ARF levels are unaffected by prolonged culture or immortalisation. These expression patterns resemble the behaviour of INK4a and ARF in human fibroblasts. However, short-hairpin RNA (shRNA)-mediated knockdown of chicken INK4b or ARF provides only modest lifespan extension, suggesting that other factors contribute to senescence in CEFs. As well as underscoring the importance of the INK4b-ARF-INK4a locus in senescence, these findings imply that the encoded products have assumed different roles in different evolutionary niches. Although ARF RNA is not detectable in early chicken embryos, the INK4b transcript is expressed in the roof-plate of the developing hind-brain, consistent with a role in limiting cell proliferation.</jats:p> Upregulation of chicken p15INK4b at senescence and in the developing brain Journal of Cell Science |
| spellingShingle | Kim, S.-H., Rowe, J., Fujii, H., Jones, R., Schmierer, B., Kong, B.-W., Kuchler, K., Foster, D., Ish-Horowicz, D., Peters, G., Journal of Cell Science, Upregulation of chicken p15INK4b at senescence and in the developing brain, Cell Biology |
| title | Upregulation of chicken p15INK4b at senescence and in the developing brain |
| title_full | Upregulation of chicken p15INK4b at senescence and in the developing brain |
| title_fullStr | Upregulation of chicken p15INK4b at senescence and in the developing brain |
| title_full_unstemmed | Upregulation of chicken p15INK4b at senescence and in the developing brain |
| title_short | Upregulation of chicken p15INK4b at senescence and in the developing brain |
| title_sort | upregulation of chicken p15ink4b at senescence and in the developing brain |
| title_unstemmed | Upregulation of chicken p15INK4b at senescence and in the developing brain |
| topic | Cell Biology |
| url | http://dx.doi.org/10.1242/jcs.02989 |