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Requirement for Pax6 in corneal morphogenesis: a role in adhesion
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| Zeitschriftentitel: | Journal of Cell Science |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Journal of Cell Science, 116, 2003, 11, S. 2157-2167 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
The Company of Biologists
|
| Schlagwörter: |
| author_facet |
Davis, Janine Duncan, Melinda K. Robison, W. Gerald Piatigorsky, Joram Davis, Janine Duncan, Melinda K. Robison, W. Gerald Piatigorsky, Joram |
|---|---|
| author |
Davis, Janine Duncan, Melinda K. Robison, W. Gerald Piatigorsky, Joram |
| spellingShingle |
Davis, Janine Duncan, Melinda K. Robison, W. Gerald Piatigorsky, Joram Journal of Cell Science Requirement for Pax6 in corneal morphogenesis: a role in adhesion Cell Biology |
| author_sort |
davis, janine |
| spelling |
Davis, Janine Duncan, Melinda K. Robison, W. Gerald Piatigorsky, Joram 1477-9137 0021-9533 The Company of Biologists Cell Biology http://dx.doi.org/10.1242/jcs.00441 <jats:p>The Pax6 transcription factor functions early during embryogenesis to control key steps in brain, pancreas, olfactory and ocular system development. A requirement for Pax6 in proper formation of lens, iris and retina is well documented. By examining the corneas of heterozygous Small eye (SEY) mice,this report shows that Pax6 is also necessary for normal corneal morphogenesis. In particular, the epithelial component of the postnatal and adult SEY (+/–) cornea is thinner owing to a reduction in the number of cell layers, despite a tenfold increase in the proliferative index and no change in TUNEL labeling. Ultrastructural views revealed large gaps between corneal epithelial cells and a change in the appearance of desmosomes,suggesting that adhesion abnormalities contribute to the corneal phenotype of SEY (+/–) mice. Western blot analysis and immunofluorescence showed equivalent amounts and normal localization of E-cadherin in SEY (+/–)corneas, and the actin cytoskeleton appeared normal as judged by phalloidin staining. By contrast, the levels of desmoglein, β-catenin andγ-catenin were reduced in the SEY (+/–) cornea. In addition, the amount of keratin-12 mRNA and protein, the major intermediate filament, was reduced in SEY (+/–) corneal epithelium as shown by in situ hybridization and immunohistochemistry. Finally, the SEY (+/–) corneal epithelium adheres less well than wild-type when challenged with gentle rubbing using a microsponge. In conclusion, our results indicate that cellular adhesion is compromised in the SEY (+/–) corneal epithelium and suggests a role for Pax6 in the proper generation and maintenance of the adult cornea.</jats:p> Requirement for Pax6 in corneal morphogenesis: a role in adhesion Journal of Cell Science |
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| title |
Requirement for Pax6 in corneal morphogenesis: a role in adhesion |
| title_unstemmed |
Requirement for Pax6 in corneal morphogenesis: a role in adhesion |
| title_full |
Requirement for Pax6 in corneal morphogenesis: a role in adhesion |
| title_fullStr |
Requirement for Pax6 in corneal morphogenesis: a role in adhesion |
| title_full_unstemmed |
Requirement for Pax6 in corneal morphogenesis: a role in adhesion |
| title_short |
Requirement for Pax6 in corneal morphogenesis: a role in adhesion |
| title_sort |
requirement for pax6 in corneal morphogenesis: a role in adhesion |
| topic |
Cell Biology |
| url |
http://dx.doi.org/10.1242/jcs.00441 |
| publishDate |
2003 |
| physical |
2157-2167 |
| description |
<jats:p>The Pax6 transcription factor functions early during embryogenesis to control key steps in brain, pancreas, olfactory and ocular system development. A requirement for Pax6 in proper formation of lens, iris and retina is well documented. By examining the corneas of heterozygous Small eye (SEY) mice,this report shows that Pax6 is also necessary for normal corneal morphogenesis. In particular, the epithelial component of the postnatal and adult SEY (+/–) cornea is thinner owing to a reduction in the number of cell layers, despite a tenfold increase in the proliferative index and no change in TUNEL labeling. Ultrastructural views revealed large gaps between corneal epithelial cells and a change in the appearance of desmosomes,suggesting that adhesion abnormalities contribute to the corneal phenotype of SEY (+/–) mice. Western blot analysis and immunofluorescence showed equivalent amounts and normal localization of E-cadherin in SEY (+/–)corneas, and the actin cytoskeleton appeared normal as judged by phalloidin staining. By contrast, the levels of desmoglein, β-catenin andγ-catenin were reduced in the SEY (+/–) cornea. In addition, the amount of keratin-12 mRNA and protein, the major intermediate filament, was reduced in SEY (+/–) corneal epithelium as shown by in situ hybridization and immunohistochemistry. Finally, the SEY (+/–) corneal epithelium adheres less well than wild-type when challenged with gentle rubbing using a microsponge. In conclusion, our results indicate that cellular adhesion is compromised in the SEY (+/–) corneal epithelium and suggests a role for Pax6 in the proper generation and maintenance of the adult cornea.</jats:p> |
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| author | Davis, Janine, Duncan, Melinda K., Robison, W. Gerald, Piatigorsky, Joram |
| author_facet | Davis, Janine, Duncan, Melinda K., Robison, W. Gerald, Piatigorsky, Joram, Davis, Janine, Duncan, Melinda K., Robison, W. Gerald, Piatigorsky, Joram |
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| description | <jats:p>The Pax6 transcription factor functions early during embryogenesis to control key steps in brain, pancreas, olfactory and ocular system development. A requirement for Pax6 in proper formation of lens, iris and retina is well documented. By examining the corneas of heterozygous Small eye (SEY) mice,this report shows that Pax6 is also necessary for normal corneal morphogenesis. In particular, the epithelial component of the postnatal and adult SEY (+/–) cornea is thinner owing to a reduction in the number of cell layers, despite a tenfold increase in the proliferative index and no change in TUNEL labeling. Ultrastructural views revealed large gaps between corneal epithelial cells and a change in the appearance of desmosomes,suggesting that adhesion abnormalities contribute to the corneal phenotype of SEY (+/–) mice. Western blot analysis and immunofluorescence showed equivalent amounts and normal localization of E-cadherin in SEY (+/–)corneas, and the actin cytoskeleton appeared normal as judged by phalloidin staining. By contrast, the levels of desmoglein, β-catenin andγ-catenin were reduced in the SEY (+/–) cornea. In addition, the amount of keratin-12 mRNA and protein, the major intermediate filament, was reduced in SEY (+/–) corneal epithelium as shown by in situ hybridization and immunohistochemistry. Finally, the SEY (+/–) corneal epithelium adheres less well than wild-type when challenged with gentle rubbing using a microsponge. In conclusion, our results indicate that cellular adhesion is compromised in the SEY (+/–) corneal epithelium and suggests a role for Pax6 in the proper generation and maintenance of the adult cornea.</jats:p> |
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| spelling | Davis, Janine Duncan, Melinda K. Robison, W. Gerald Piatigorsky, Joram 1477-9137 0021-9533 The Company of Biologists Cell Biology http://dx.doi.org/10.1242/jcs.00441 <jats:p>The Pax6 transcription factor functions early during embryogenesis to control key steps in brain, pancreas, olfactory and ocular system development. A requirement for Pax6 in proper formation of lens, iris and retina is well documented. By examining the corneas of heterozygous Small eye (SEY) mice,this report shows that Pax6 is also necessary for normal corneal morphogenesis. In particular, the epithelial component of the postnatal and adult SEY (+/–) cornea is thinner owing to a reduction in the number of cell layers, despite a tenfold increase in the proliferative index and no change in TUNEL labeling. Ultrastructural views revealed large gaps between corneal epithelial cells and a change in the appearance of desmosomes,suggesting that adhesion abnormalities contribute to the corneal phenotype of SEY (+/–) mice. Western blot analysis and immunofluorescence showed equivalent amounts and normal localization of E-cadherin in SEY (+/–)corneas, and the actin cytoskeleton appeared normal as judged by phalloidin staining. By contrast, the levels of desmoglein, β-catenin andγ-catenin were reduced in the SEY (+/–) cornea. In addition, the amount of keratin-12 mRNA and protein, the major intermediate filament, was reduced in SEY (+/–) corneal epithelium as shown by in situ hybridization and immunohistochemistry. Finally, the SEY (+/–) corneal epithelium adheres less well than wild-type when challenged with gentle rubbing using a microsponge. In conclusion, our results indicate that cellular adhesion is compromised in the SEY (+/–) corneal epithelium and suggests a role for Pax6 in the proper generation and maintenance of the adult cornea.</jats:p> Requirement for Pax6 in corneal morphogenesis: a role in adhesion Journal of Cell Science |
| spellingShingle | Davis, Janine, Duncan, Melinda K., Robison, W. Gerald, Piatigorsky, Joram, Journal of Cell Science, Requirement for Pax6 in corneal morphogenesis: a role in adhesion, Cell Biology |
| title | Requirement for Pax6 in corneal morphogenesis: a role in adhesion |
| title_full | Requirement for Pax6 in corneal morphogenesis: a role in adhesion |
| title_fullStr | Requirement for Pax6 in corneal morphogenesis: a role in adhesion |
| title_full_unstemmed | Requirement for Pax6 in corneal morphogenesis: a role in adhesion |
| title_short | Requirement for Pax6 in corneal morphogenesis: a role in adhesion |
| title_sort | requirement for pax6 in corneal morphogenesis: a role in adhesion |
| title_unstemmed | Requirement for Pax6 in corneal morphogenesis: a role in adhesion |
| topic | Cell Biology |
| url | http://dx.doi.org/10.1242/jcs.00441 |