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Caenorhabditis elegansTwist plays an essential role in non-striated muscle development
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| Zeitschriftentitel: | Development |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Development, 127, 2000, 10, S. 2041-2051 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
The Company of Biologists
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| Schlagwörter: |
| author_facet |
Corsi, Ann K. Kostas, Stephen A. Fire, Andrew Krause, Michael Corsi, Ann K. Kostas, Stephen A. Fire, Andrew Krause, Michael |
|---|---|
| author |
Corsi, Ann K. Kostas, Stephen A. Fire, Andrew Krause, Michael |
| spellingShingle |
Corsi, Ann K. Kostas, Stephen A. Fire, Andrew Krause, Michael Development Caenorhabditis elegansTwist plays an essential role in non-striated muscle development Developmental Biology Molecular Biology |
| author_sort |
corsi, ann k. |
| spelling |
Corsi, Ann K. Kostas, Stephen A. Fire, Andrew Krause, Michael 0950-1991 1477-9129 The Company of Biologists Developmental Biology Molecular Biology http://dx.doi.org/10.1242/dev.127.10.2041 <jats:title>ABSTRACT</jats:title><jats:p>The basic helix-loop-helix (bHLH) transcription factor Twist plays a role in mesodermal development in both invertebrates and vertebrates. In an effort to understand the role of the unique Caenorhabditis elegans Twist homolog, hlh-8, we analyzed mesodermal development in animals with a deletion in the hlh-8 locus. This deletion was predicted to represent a null allele because the HLH domain is missing and the reading frame for the protein is disrupted. Animals lacking CeTwist function were constipated and egg-laying defective. Both of these defects were rescued in transgenic mutant animals expressing wild-type hlh-8. Observing a series of mesoderm-specific markers allowed us to characterize the loss of hlh-8 function more thoroughly. Our results demonstrate that CeTwist performs an essential role in the proper development of a subset of mesodermal tissues in C. elegans. We found that CeTwist was required for the formation of three out of the four non-striated enteric muscles born in the embryo. In contrast, CeTwist was not required for the formation of the embryonically derived striated muscles. Most of the post-embryonic mesoderm develops from a single lineage. CeTwist was necessary for appropriate patterning in this lineage and was required for expression of two downstream target genes, but was not required for the expression of myosin, a marker of differentiation. Our results suggest that mesodermal patterning by Twist is an evolutionarily conserved function.</jats:p> <i>Caenorhabditis elegans</i>Twist plays an essential role in non-striated muscle development Development |
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Caenorhabditis elegansTwist plays an essential role in non-striated muscle development |
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Caenorhabditis elegansTwist plays an essential role in non-striated muscle development |
| title_full |
Caenorhabditis elegansTwist plays an essential role in non-striated muscle development |
| title_fullStr |
Caenorhabditis elegansTwist plays an essential role in non-striated muscle development |
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Caenorhabditis elegansTwist plays an essential role in non-striated muscle development |
| title_short |
Caenorhabditis elegansTwist plays an essential role in non-striated muscle development |
| title_sort |
<i>caenorhabditis elegans</i>twist plays an essential role in non-striated muscle development |
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Developmental Biology Molecular Biology |
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http://dx.doi.org/10.1242/dev.127.10.2041 |
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2000 |
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2041-2051 |
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<jats:title>ABSTRACT</jats:title><jats:p>The basic helix-loop-helix (bHLH) transcription factor Twist plays a role in mesodermal development in both invertebrates and vertebrates. In an effort to understand the role of the unique Caenorhabditis elegans Twist homolog, hlh-8, we analyzed mesodermal development in animals with a deletion in the hlh-8 locus. This deletion was predicted to represent a null allele because the HLH domain is missing and the reading frame for the protein is disrupted. Animals lacking CeTwist function were constipated and egg-laying defective. Both of these defects were rescued in transgenic mutant animals expressing wild-type hlh-8. Observing a series of mesoderm-specific markers allowed us to characterize the loss of hlh-8 function more thoroughly. Our results demonstrate that CeTwist performs an essential role in the proper development of a subset of mesodermal tissues in C. elegans. We found that CeTwist was required for the formation of three out of the four non-striated enteric muscles born in the embryo. In contrast, CeTwist was not required for the formation of the embryonically derived striated muscles. Most of the post-embryonic mesoderm develops from a single lineage. CeTwist was necessary for appropriate patterning in this lineage and was required for expression of two downstream target genes, but was not required for the expression of myosin, a marker of differentiation. Our results suggest that mesodermal patterning by Twist is an evolutionarily conserved function.</jats:p> |
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| author | Corsi, Ann K., Kostas, Stephen A., Fire, Andrew, Krause, Michael |
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| description | <jats:title>ABSTRACT</jats:title><jats:p>The basic helix-loop-helix (bHLH) transcription factor Twist plays a role in mesodermal development in both invertebrates and vertebrates. In an effort to understand the role of the unique Caenorhabditis elegans Twist homolog, hlh-8, we analyzed mesodermal development in animals with a deletion in the hlh-8 locus. This deletion was predicted to represent a null allele because the HLH domain is missing and the reading frame for the protein is disrupted. Animals lacking CeTwist function were constipated and egg-laying defective. Both of these defects were rescued in transgenic mutant animals expressing wild-type hlh-8. Observing a series of mesoderm-specific markers allowed us to characterize the loss of hlh-8 function more thoroughly. Our results demonstrate that CeTwist performs an essential role in the proper development of a subset of mesodermal tissues in C. elegans. We found that CeTwist was required for the formation of three out of the four non-striated enteric muscles born in the embryo. In contrast, CeTwist was not required for the formation of the embryonically derived striated muscles. Most of the post-embryonic mesoderm develops from a single lineage. CeTwist was necessary for appropriate patterning in this lineage and was required for expression of two downstream target genes, but was not required for the expression of myosin, a marker of differentiation. Our results suggest that mesodermal patterning by Twist is an evolutionarily conserved function.</jats:p> |
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| spelling | Corsi, Ann K. Kostas, Stephen A. Fire, Andrew Krause, Michael 0950-1991 1477-9129 The Company of Biologists Developmental Biology Molecular Biology http://dx.doi.org/10.1242/dev.127.10.2041 <jats:title>ABSTRACT</jats:title><jats:p>The basic helix-loop-helix (bHLH) transcription factor Twist plays a role in mesodermal development in both invertebrates and vertebrates. In an effort to understand the role of the unique Caenorhabditis elegans Twist homolog, hlh-8, we analyzed mesodermal development in animals with a deletion in the hlh-8 locus. This deletion was predicted to represent a null allele because the HLH domain is missing and the reading frame for the protein is disrupted. Animals lacking CeTwist function were constipated and egg-laying defective. Both of these defects were rescued in transgenic mutant animals expressing wild-type hlh-8. Observing a series of mesoderm-specific markers allowed us to characterize the loss of hlh-8 function more thoroughly. Our results demonstrate that CeTwist performs an essential role in the proper development of a subset of mesodermal tissues in C. elegans. We found that CeTwist was required for the formation of three out of the four non-striated enteric muscles born in the embryo. In contrast, CeTwist was not required for the formation of the embryonically derived striated muscles. Most of the post-embryonic mesoderm develops from a single lineage. CeTwist was necessary for appropriate patterning in this lineage and was required for expression of two downstream target genes, but was not required for the expression of myosin, a marker of differentiation. Our results suggest that mesodermal patterning by Twist is an evolutionarily conserved function.</jats:p> <i>Caenorhabditis elegans</i>Twist plays an essential role in non-striated muscle development Development |
| spellingShingle | Corsi, Ann K., Kostas, Stephen A., Fire, Andrew, Krause, Michael, Development, Caenorhabditis elegansTwist plays an essential role in non-striated muscle development, Developmental Biology, Molecular Biology |
| title | Caenorhabditis elegansTwist plays an essential role in non-striated muscle development |
| title_full | Caenorhabditis elegansTwist plays an essential role in non-striated muscle development |
| title_fullStr | Caenorhabditis elegansTwist plays an essential role in non-striated muscle development |
| title_full_unstemmed | Caenorhabditis elegansTwist plays an essential role in non-striated muscle development |
| title_short | Caenorhabditis elegansTwist plays an essential role in non-striated muscle development |
| title_sort | <i>caenorhabditis elegans</i>twist plays an essential role in non-striated muscle development |
| title_unstemmed | Caenorhabditis elegansTwist plays an essential role in non-striated muscle development |
| topic | Developmental Biology, Molecular Biology |
| url | http://dx.doi.org/10.1242/dev.127.10.2041 |