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Pancreatic neurogenin 3-expressing cells are unipotent islet precursors
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| Zeitschriftentitel: | Development |
|---|---|
| Personen und Körperschaften: | , |
| In: | Development, 136, 2009, 21, S. 3567-3574 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
The Company of Biologists
|
| Schlagwörter: |
| author_facet |
Desgraz, Renaud Herrera, Pedro L. Desgraz, Renaud Herrera, Pedro L. |
|---|---|
| author |
Desgraz, Renaud Herrera, Pedro L. |
| spellingShingle |
Desgraz, Renaud Herrera, Pedro L. Development Pancreatic neurogenin 3-expressing cells are unipotent islet precursors Developmental Biology Molecular Biology |
| author_sort |
desgraz, renaud |
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Desgraz, Renaud Herrera, Pedro L. 1477-9129 0950-1991 The Company of Biologists Developmental Biology Molecular Biology http://dx.doi.org/10.1242/dev.039214 <jats:p>Pancreatic islet endocrine cells arise during development from precursors expressing neurogenin 3 (Ngn3). As a population, Ngn3+ cells produce all islet cell types, but the potential of individual Ngn3+cells, an issue central to organogenesis in general and to in vitro differentiation towards cell-based therapies, has not been addressed. We performed in vivo clonal analyses in mice to study the proliferation and differentiation of very large numbers of single Ngn3+ cells using MADM, a genetic system in which a Cre-dependent chromosomal translocation labels, at extremely low mosaic efficiency, a small number of Ngn3+cells. We scored large numbers of progeny arising from single Ngn3+cells. In newborns, labeled islets frequently contained just a single tagged endocrine cell, indicating for the first time that each Ngn3+ cell is the precursor of a single endocrine cell. In adults, small clusters of two to three Ngn3+ progeny were detected, but all expressed the same hormone, indicating a low rate of replication from birth to adult stages. We propose a model whereby Ngn3+ cells are monotypic (i.e. unipotent)precursors, and use this paradigm to refocus ideas on how cell number and type must be regulated in building complete islets of Langerhans.</jats:p> Pancreatic neurogenin 3-expressing cells are unipotent islet precursors Development |
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The Company of Biologists, 2009 |
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| title |
Pancreatic neurogenin 3-expressing cells are unipotent islet precursors |
| title_unstemmed |
Pancreatic neurogenin 3-expressing cells are unipotent islet precursors |
| title_full |
Pancreatic neurogenin 3-expressing cells are unipotent islet precursors |
| title_fullStr |
Pancreatic neurogenin 3-expressing cells are unipotent islet precursors |
| title_full_unstemmed |
Pancreatic neurogenin 3-expressing cells are unipotent islet precursors |
| title_short |
Pancreatic neurogenin 3-expressing cells are unipotent islet precursors |
| title_sort |
pancreatic neurogenin 3-expressing cells are unipotent islet precursors |
| topic |
Developmental Biology Molecular Biology |
| url |
http://dx.doi.org/10.1242/dev.039214 |
| publishDate |
2009 |
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3567-3574 |
| description |
<jats:p>Pancreatic islet endocrine cells arise during development from precursors expressing neurogenin 3 (Ngn3). As a population, Ngn3+ cells produce all islet cell types, but the potential of individual Ngn3+cells, an issue central to organogenesis in general and to in vitro differentiation towards cell-based therapies, has not been addressed. We performed in vivo clonal analyses in mice to study the proliferation and differentiation of very large numbers of single Ngn3+ cells using MADM, a genetic system in which a Cre-dependent chromosomal translocation labels, at extremely low mosaic efficiency, a small number of Ngn3+cells. We scored large numbers of progeny arising from single Ngn3+cells. In newborns, labeled islets frequently contained just a single tagged endocrine cell, indicating for the first time that each Ngn3+ cell is the precursor of a single endocrine cell. In adults, small clusters of two to three Ngn3+ progeny were detected, but all expressed the same hormone, indicating a low rate of replication from birth to adult stages. We propose a model whereby Ngn3+ cells are monotypic (i.e. unipotent)precursors, and use this paradigm to refocus ideas on how cell number and type must be regulated in building complete islets of Langerhans.</jats:p> |
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| author | Desgraz, Renaud, Herrera, Pedro L. |
| author_facet | Desgraz, Renaud, Herrera, Pedro L., Desgraz, Renaud, Herrera, Pedro L. |
| author_sort | desgraz, renaud |
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| description | <jats:p>Pancreatic islet endocrine cells arise during development from precursors expressing neurogenin 3 (Ngn3). As a population, Ngn3+ cells produce all islet cell types, but the potential of individual Ngn3+cells, an issue central to organogenesis in general and to in vitro differentiation towards cell-based therapies, has not been addressed. We performed in vivo clonal analyses in mice to study the proliferation and differentiation of very large numbers of single Ngn3+ cells using MADM, a genetic system in which a Cre-dependent chromosomal translocation labels, at extremely low mosaic efficiency, a small number of Ngn3+cells. We scored large numbers of progeny arising from single Ngn3+cells. In newborns, labeled islets frequently contained just a single tagged endocrine cell, indicating for the first time that each Ngn3+ cell is the precursor of a single endocrine cell. In adults, small clusters of two to three Ngn3+ progeny were detected, but all expressed the same hormone, indicating a low rate of replication from birth to adult stages. We propose a model whereby Ngn3+ cells are monotypic (i.e. unipotent)precursors, and use this paradigm to refocus ideas on how cell number and type must be regulated in building complete islets of Langerhans.</jats:p> |
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| spelling | Desgraz, Renaud Herrera, Pedro L. 1477-9129 0950-1991 The Company of Biologists Developmental Biology Molecular Biology http://dx.doi.org/10.1242/dev.039214 <jats:p>Pancreatic islet endocrine cells arise during development from precursors expressing neurogenin 3 (Ngn3). As a population, Ngn3+ cells produce all islet cell types, but the potential of individual Ngn3+cells, an issue central to organogenesis in general and to in vitro differentiation towards cell-based therapies, has not been addressed. We performed in vivo clonal analyses in mice to study the proliferation and differentiation of very large numbers of single Ngn3+ cells using MADM, a genetic system in which a Cre-dependent chromosomal translocation labels, at extremely low mosaic efficiency, a small number of Ngn3+cells. We scored large numbers of progeny arising from single Ngn3+cells. In newborns, labeled islets frequently contained just a single tagged endocrine cell, indicating for the first time that each Ngn3+ cell is the precursor of a single endocrine cell. In adults, small clusters of two to three Ngn3+ progeny were detected, but all expressed the same hormone, indicating a low rate of replication from birth to adult stages. We propose a model whereby Ngn3+ cells are monotypic (i.e. unipotent)precursors, and use this paradigm to refocus ideas on how cell number and type must be regulated in building complete islets of Langerhans.</jats:p> Pancreatic neurogenin 3-expressing cells are unipotent islet precursors Development |
| spellingShingle | Desgraz, Renaud, Herrera, Pedro L., Development, Pancreatic neurogenin 3-expressing cells are unipotent islet precursors, Developmental Biology, Molecular Biology |
| title | Pancreatic neurogenin 3-expressing cells are unipotent islet precursors |
| title_full | Pancreatic neurogenin 3-expressing cells are unipotent islet precursors |
| title_fullStr | Pancreatic neurogenin 3-expressing cells are unipotent islet precursors |
| title_full_unstemmed | Pancreatic neurogenin 3-expressing cells are unipotent islet precursors |
| title_short | Pancreatic neurogenin 3-expressing cells are unipotent islet precursors |
| title_sort | pancreatic neurogenin 3-expressing cells are unipotent islet precursors |
| title_unstemmed | Pancreatic neurogenin 3-expressing cells are unipotent islet precursors |
| topic | Developmental Biology, Molecular Biology |
| url | http://dx.doi.org/10.1242/dev.039214 |