Eintrag weiter verarbeiten
Squamous cell carcinoma and mammary abscess formation through squamous metaplasia inSmad4/Dpc4conditional knockout mice
Gespeichert in:
| Zeitschriftentitel: | Development |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , |
| In: | Development, 130, 2003, 24, S. 6143-6153 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
The Company of Biologists
|
| Schlagwörter: |
| author_facet |
Li, Wenmei Qiao, Wenhui Chen, Lin Xu, Xiaoling Yang, Xiao Li, Dan Li, Cuiling Brodie, Steven G. Meguid, Michael M. Hennighausen, Lothar Deng, Chu-Xia Li, Wenmei Qiao, Wenhui Chen, Lin Xu, Xiaoling Yang, Xiao Li, Dan Li, Cuiling Brodie, Steven G. Meguid, Michael M. Hennighausen, Lothar Deng, Chu-Xia |
|---|---|
| author |
Li, Wenmei Qiao, Wenhui Chen, Lin Xu, Xiaoling Yang, Xiao Li, Dan Li, Cuiling Brodie, Steven G. Meguid, Michael M. Hennighausen, Lothar Deng, Chu-Xia |
| spellingShingle |
Li, Wenmei Qiao, Wenhui Chen, Lin Xu, Xiaoling Yang, Xiao Li, Dan Li, Cuiling Brodie, Steven G. Meguid, Michael M. Hennighausen, Lothar Deng, Chu-Xia Development Squamous cell carcinoma and mammary abscess formation through squamous metaplasia inSmad4/Dpc4conditional knockout mice Developmental Biology Molecular Biology |
| author_sort |
li, wenmei |
| spelling |
Li, Wenmei Qiao, Wenhui Chen, Lin Xu, Xiaoling Yang, Xiao Li, Dan Li, Cuiling Brodie, Steven G. Meguid, Michael M. Hennighausen, Lothar Deng, Chu-Xia 1477-9129 0950-1991 The Company of Biologists Developmental Biology Molecular Biology http://dx.doi.org/10.1242/dev.00820 <jats:p>Smad4 is a central mediator for TGFβ signals, which play important functions in many biological processes. To study the role of Smad4 in mammary gland development and neoplasia, we disrupted this gene in mammary epithelium using a Cre-loxP approach. Smad4 is expressed in the mammary gland throughout development; however, its inactivation did not cause abnormal development of the gland during the first three pregnancies. Instead, lack of Smad4 gradually induced cell proliferation, alveolar hyperplasia and transdifferentiation of mammary epithelial cells into squamous epithelial cells. Consequently, all mutant mice developed squamous cell carcinoma and/or mammary abscesses between 5 and 16 months of age. We demonstrated that absence of Smad4 resulted in β-catenin accumulation at onset and throughout the process of transdifferentiation, implicating β-catenin, a key component of the Wnt signaling pathway, in the development of squamous metaplasia in Smad4-null mammary glands. We further demonstrated that TGFβ1 treatment degraded β-catenin and induced epithelial-mesenchymal transformation in cultured mammary epithelial cells. However, such actions were blocked in the absence of Smad4. These findings indicate that TGFβ/Smad4 signals play a role in cell fate maintenance during mammary gland development and neoplasia.</jats:p> Squamous cell carcinoma and mammary abscess formation through squamous metaplasia in<i>Smad4/Dpc4</i>conditional knockout mice Development |
| doi_str_mv |
10.1242/dev.00820 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTI0Mi9kZXYuMDA4MjA |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTI0Mi9kZXYuMDA4MjA |
| institution |
DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 |
| imprint |
The Company of Biologists, 2003 |
| imprint_str_mv |
The Company of Biologists, 2003 |
| issn |
1477-9129 0950-1991 |
| issn_str_mv |
1477-9129 0950-1991 |
| language |
English |
| mega_collection |
The Company of Biologists (CrossRef) |
| match_str |
li2003squamouscellcarcinomaandmammaryabscessformationthroughsquamousmetaplasiainsmad4dpc4conditionalknockoutmice |
| publishDateSort |
2003 |
| publisher |
The Company of Biologists |
| recordtype |
ai |
| record_format |
ai |
| series |
Development |
| source_id |
49 |
| title |
Squamous cell carcinoma and mammary abscess formation through squamous metaplasia inSmad4/Dpc4conditional knockout mice |
| title_unstemmed |
Squamous cell carcinoma and mammary abscess formation through squamous metaplasia inSmad4/Dpc4conditional knockout mice |
| title_full |
Squamous cell carcinoma and mammary abscess formation through squamous metaplasia inSmad4/Dpc4conditional knockout mice |
| title_fullStr |
Squamous cell carcinoma and mammary abscess formation through squamous metaplasia inSmad4/Dpc4conditional knockout mice |
| title_full_unstemmed |
Squamous cell carcinoma and mammary abscess formation through squamous metaplasia inSmad4/Dpc4conditional knockout mice |
| title_short |
Squamous cell carcinoma and mammary abscess formation through squamous metaplasia inSmad4/Dpc4conditional knockout mice |
| title_sort |
squamous cell carcinoma and mammary abscess formation through squamous metaplasia in<i>smad4/dpc4</i>conditional knockout mice |
| topic |
Developmental Biology Molecular Biology |
| url |
http://dx.doi.org/10.1242/dev.00820 |
| publishDate |
2003 |
| physical |
6143-6153 |
| description |
<jats:p>Smad4 is a central mediator for TGFβ signals, which play important functions in many biological processes. To study the role of Smad4 in mammary gland development and neoplasia, we disrupted this gene in mammary epithelium using a Cre-loxP approach. Smad4 is expressed in the mammary gland throughout development; however, its inactivation did not cause abnormal development of the gland during the first three pregnancies. Instead, lack of Smad4 gradually induced cell proliferation, alveolar hyperplasia and transdifferentiation of mammary epithelial cells into squamous epithelial cells. Consequently, all mutant mice developed squamous cell carcinoma and/or mammary abscesses between 5 and 16 months of age. We demonstrated that absence of Smad4 resulted in β-catenin accumulation at onset and throughout the process of transdifferentiation, implicating β-catenin, a key component of the Wnt signaling pathway, in the development of squamous metaplasia in Smad4-null mammary glands. We further demonstrated that TGFβ1 treatment degraded β-catenin and induced epithelial-mesenchymal transformation in cultured mammary epithelial cells. However, such actions were blocked in the absence of Smad4. These findings indicate that TGFβ/Smad4 signals play a role in cell fate maintenance during mammary gland development and neoplasia.</jats:p> |
| container_issue |
24 |
| container_start_page |
6143 |
| container_title |
Development |
| container_volume |
130 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792344415471140864 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T17:07:12.639Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Squamous+cell+carcinoma+and+mammary+abscess+formation+through+squamous+metaplasia+inSmad4%2FDpc4conditional+knockout+mice&rft.date=2003-12-15&genre=article&issn=0950-1991&volume=130&issue=24&spage=6143&epage=6153&pages=6143-6153&jtitle=Development&atitle=Squamous+cell+carcinoma+and+mammary+abscess+formation+through+squamous+metaplasia+in%3Ci%3ESmad4%2FDpc4%3C%2Fi%3Econditional+knockout+mice&aulast=Deng&aufirst=Chu-Xia&rft_id=info%3Adoi%2F10.1242%2Fdev.00820&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792344415471140864 |
| author | Li, Wenmei, Qiao, Wenhui, Chen, Lin, Xu, Xiaoling, Yang, Xiao, Li, Dan, Li, Cuiling, Brodie, Steven G., Meguid, Michael M., Hennighausen, Lothar, Deng, Chu-Xia |
| author_facet | Li, Wenmei, Qiao, Wenhui, Chen, Lin, Xu, Xiaoling, Yang, Xiao, Li, Dan, Li, Cuiling, Brodie, Steven G., Meguid, Michael M., Hennighausen, Lothar, Deng, Chu-Xia, Li, Wenmei, Qiao, Wenhui, Chen, Lin, Xu, Xiaoling, Yang, Xiao, Li, Dan, Li, Cuiling, Brodie, Steven G., Meguid, Michael M., Hennighausen, Lothar, Deng, Chu-Xia |
| author_sort | li, wenmei |
| container_issue | 24 |
| container_start_page | 6143 |
| container_title | Development |
| container_volume | 130 |
| description | <jats:p>Smad4 is a central mediator for TGFβ signals, which play important functions in many biological processes. To study the role of Smad4 in mammary gland development and neoplasia, we disrupted this gene in mammary epithelium using a Cre-loxP approach. Smad4 is expressed in the mammary gland throughout development; however, its inactivation did not cause abnormal development of the gland during the first three pregnancies. Instead, lack of Smad4 gradually induced cell proliferation, alveolar hyperplasia and transdifferentiation of mammary epithelial cells into squamous epithelial cells. Consequently, all mutant mice developed squamous cell carcinoma and/or mammary abscesses between 5 and 16 months of age. We demonstrated that absence of Smad4 resulted in β-catenin accumulation at onset and throughout the process of transdifferentiation, implicating β-catenin, a key component of the Wnt signaling pathway, in the development of squamous metaplasia in Smad4-null mammary glands. We further demonstrated that TGFβ1 treatment degraded β-catenin and induced epithelial-mesenchymal transformation in cultured mammary epithelial cells. However, such actions were blocked in the absence of Smad4. These findings indicate that TGFβ/Smad4 signals play a role in cell fate maintenance during mammary gland development and neoplasia.</jats:p> |
| doi_str_mv | 10.1242/dev.00820 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTI0Mi9kZXYuMDA4MjA |
| imprint | The Company of Biologists, 2003 |
| imprint_str_mv | The Company of Biologists, 2003 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
| issn | 1477-9129, 0950-1991 |
| issn_str_mv | 1477-9129, 0950-1991 |
| language | English |
| last_indexed | 2024-03-01T17:07:12.639Z |
| match_str | li2003squamouscellcarcinomaandmammaryabscessformationthroughsquamousmetaplasiainsmad4dpc4conditionalknockoutmice |
| mega_collection | The Company of Biologists (CrossRef) |
| physical | 6143-6153 |
| publishDate | 2003 |
| publishDateSort | 2003 |
| publisher | The Company of Biologists |
| record_format | ai |
| recordtype | ai |
| series | Development |
| source_id | 49 |
| spelling | Li, Wenmei Qiao, Wenhui Chen, Lin Xu, Xiaoling Yang, Xiao Li, Dan Li, Cuiling Brodie, Steven G. Meguid, Michael M. Hennighausen, Lothar Deng, Chu-Xia 1477-9129 0950-1991 The Company of Biologists Developmental Biology Molecular Biology http://dx.doi.org/10.1242/dev.00820 <jats:p>Smad4 is a central mediator for TGFβ signals, which play important functions in many biological processes. To study the role of Smad4 in mammary gland development and neoplasia, we disrupted this gene in mammary epithelium using a Cre-loxP approach. Smad4 is expressed in the mammary gland throughout development; however, its inactivation did not cause abnormal development of the gland during the first three pregnancies. Instead, lack of Smad4 gradually induced cell proliferation, alveolar hyperplasia and transdifferentiation of mammary epithelial cells into squamous epithelial cells. Consequently, all mutant mice developed squamous cell carcinoma and/or mammary abscesses between 5 and 16 months of age. We demonstrated that absence of Smad4 resulted in β-catenin accumulation at onset and throughout the process of transdifferentiation, implicating β-catenin, a key component of the Wnt signaling pathway, in the development of squamous metaplasia in Smad4-null mammary glands. We further demonstrated that TGFβ1 treatment degraded β-catenin and induced epithelial-mesenchymal transformation in cultured mammary epithelial cells. However, such actions were blocked in the absence of Smad4. These findings indicate that TGFβ/Smad4 signals play a role in cell fate maintenance during mammary gland development and neoplasia.</jats:p> Squamous cell carcinoma and mammary abscess formation through squamous metaplasia in<i>Smad4/Dpc4</i>conditional knockout mice Development |
| spellingShingle | Li, Wenmei, Qiao, Wenhui, Chen, Lin, Xu, Xiaoling, Yang, Xiao, Li, Dan, Li, Cuiling, Brodie, Steven G., Meguid, Michael M., Hennighausen, Lothar, Deng, Chu-Xia, Development, Squamous cell carcinoma and mammary abscess formation through squamous metaplasia inSmad4/Dpc4conditional knockout mice, Developmental Biology, Molecular Biology |
| title | Squamous cell carcinoma and mammary abscess formation through squamous metaplasia inSmad4/Dpc4conditional knockout mice |
| title_full | Squamous cell carcinoma and mammary abscess formation through squamous metaplasia inSmad4/Dpc4conditional knockout mice |
| title_fullStr | Squamous cell carcinoma and mammary abscess formation through squamous metaplasia inSmad4/Dpc4conditional knockout mice |
| title_full_unstemmed | Squamous cell carcinoma and mammary abscess formation through squamous metaplasia inSmad4/Dpc4conditional knockout mice |
| title_short | Squamous cell carcinoma and mammary abscess formation through squamous metaplasia inSmad4/Dpc4conditional knockout mice |
| title_sort | squamous cell carcinoma and mammary abscess formation through squamous metaplasia in<i>smad4/dpc4</i>conditional knockout mice |
| title_unstemmed | Squamous cell carcinoma and mammary abscess formation through squamous metaplasia inSmad4/Dpc4conditional knockout mice |
| topic | Developmental Biology, Molecular Biology |
| url | http://dx.doi.org/10.1242/dev.00820 |