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Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in Saccharomyces cerevisiae.
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| Zeitschriftentitel: | Molecular and Cellular Biology |
|---|---|
| Personen und Körperschaften: | |
| In: | Molecular and Cellular Biology, 2, 1982, 9, S. 1080-1087 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Informa UK Limited
|
| Schlagwörter: |
| author_facet |
Wood, J S Wood, J S |
|---|---|
| author |
Wood, J S |
| spellingShingle |
Wood, J S Molecular and Cellular Biology Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in Saccharomyces cerevisiae. Cell Biology Molecular Biology |
| author_sort |
wood, j s |
| spelling |
Wood, J S 0270-7306 1098-5549 Informa UK Limited Cell Biology Molecular Biology http://dx.doi.org/10.1128/mcb.2.9.1080 <jats:p>Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate has been utilized as a rapid and simple method for assigning genes to individual chromosomes in Saccharomyces cerevisiae. This technique relied on the segregation of heterozygous markers in a diploid strain after methyl benzimidazole-2-yl-carbamate treatment due to loss of whole chromosomes. Correlations between the expression of an unmapped gene and that of a previously mapped recessive marker indicated chromosomal linkage. Depending on whether the unmapped gene and the marker were located in coupling or in repulsion, either positive or negative correlations were seen. The chromosomal location of several previously mapped genes were confirmed as a test of the method, and one previously unmapped gene, nib1, was mapped.</jats:p> Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in Saccharomyces cerevisiae. Molecular and Cellular Biology |
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Informa UK Limited, 1982 |
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Informa UK Limited, 1982 |
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Molecular and Cellular Biology |
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| title |
Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in Saccharomyces cerevisiae. |
| title_unstemmed |
Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in Saccharomyces cerevisiae. |
| title_full |
Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in Saccharomyces cerevisiae. |
| title_fullStr |
Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in Saccharomyces cerevisiae. |
| title_full_unstemmed |
Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in Saccharomyces cerevisiae. |
| title_short |
Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in Saccharomyces cerevisiae. |
| title_sort |
mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in saccharomyces cerevisiae. |
| topic |
Cell Biology Molecular Biology |
| url |
http://dx.doi.org/10.1128/mcb.2.9.1080 |
| publishDate |
1982 |
| physical |
1080-1087 |
| description |
<jats:p>Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate has been utilized as a rapid and simple method for assigning genes to individual chromosomes in Saccharomyces cerevisiae. This technique relied on the segregation of heterozygous markers in a diploid strain after methyl benzimidazole-2-yl-carbamate treatment due to loss of whole chromosomes. Correlations between the expression of an unmapped gene and that of a previously mapped recessive marker indicated chromosomal linkage. Depending on whether the unmapped gene and the marker were located in coupling or in repulsion, either positive or negative correlations were seen. The chromosomal location of several previously mapped genes were confirmed as a test of the method, and one previously unmapped gene, nib1, was mapped.</jats:p> |
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| description | <jats:p>Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate has been utilized as a rapid and simple method for assigning genes to individual chromosomes in Saccharomyces cerevisiae. This technique relied on the segregation of heterozygous markers in a diploid strain after methyl benzimidazole-2-yl-carbamate treatment due to loss of whole chromosomes. Correlations between the expression of an unmapped gene and that of a previously mapped recessive marker indicated chromosomal linkage. Depending on whether the unmapped gene and the marker were located in coupling or in repulsion, either positive or negative correlations were seen. The chromosomal location of several previously mapped genes were confirmed as a test of the method, and one previously unmapped gene, nib1, was mapped.</jats:p> |
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| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9tY2IuMi45LjEwODA |
| imprint | Informa UK Limited, 1982 |
| imprint_str_mv | Informa UK Limited, 1982 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
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| physical | 1080-1087 |
| publishDate | 1982 |
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| series | Molecular and Cellular Biology |
| source_id | 49 |
| spelling | Wood, J S 0270-7306 1098-5549 Informa UK Limited Cell Biology Molecular Biology http://dx.doi.org/10.1128/mcb.2.9.1080 <jats:p>Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate has been utilized as a rapid and simple method for assigning genes to individual chromosomes in Saccharomyces cerevisiae. This technique relied on the segregation of heterozygous markers in a diploid strain after methyl benzimidazole-2-yl-carbamate treatment due to loss of whole chromosomes. Correlations between the expression of an unmapped gene and that of a previously mapped recessive marker indicated chromosomal linkage. Depending on whether the unmapped gene and the marker were located in coupling or in repulsion, either positive or negative correlations were seen. The chromosomal location of several previously mapped genes were confirmed as a test of the method, and one previously unmapped gene, nib1, was mapped.</jats:p> Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in Saccharomyces cerevisiae. Molecular and Cellular Biology |
| spellingShingle | Wood, J S, Molecular and Cellular Biology, Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in Saccharomyces cerevisiae., Cell Biology, Molecular Biology |
| title | Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in Saccharomyces cerevisiae. |
| title_full | Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in Saccharomyces cerevisiae. |
| title_fullStr | Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in Saccharomyces cerevisiae. |
| title_full_unstemmed | Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in Saccharomyces cerevisiae. |
| title_short | Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in Saccharomyces cerevisiae. |
| title_sort | mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in saccharomyces cerevisiae. |
| title_unstemmed | Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in Saccharomyces cerevisiae. |
| topic | Cell Biology, Molecular Biology |
| url | http://dx.doi.org/10.1128/mcb.2.9.1080 |