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A Virus-Encoded Inhibitor That Blocks RNA Interference in Mammalian Cells
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| Zeitschriftentitel: | Journal of Virology |
|---|---|
| Personen und Körperschaften: | , |
| In: | Journal of Virology, 79, 2005, 12, S. 7371-7379 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Sullivan, Christopher S. Ganem, Don Sullivan, Christopher S. Ganem, Don |
|---|---|
| author |
Sullivan, Christopher S. Ganem, Don |
| spellingShingle |
Sullivan, Christopher S. Ganem, Don Journal of Virology A Virus-Encoded Inhibitor That Blocks RNA Interference in Mammalian Cells Virology Insect Science Immunology Microbiology |
| author_sort |
sullivan, christopher s. |
| spelling |
Sullivan, Christopher S. Ganem, Don 0022-538X 1098-5514 American Society for Microbiology Virology Insect Science Immunology Microbiology http://dx.doi.org/10.1128/jvi.79.12.7371-7379.2005 <jats:title>ABSTRACT</jats:title> <jats:p>Nodamura virus (NoV) is a small RNA virus that is infectious for insect and mammalian hosts. We have developed a highly sensitive assay of RNA interference (RNAi) in mammalian cells that shows that the NoV B2 protein functions as an inhibitor of RNAi triggered by either short hairpin RNAs or small interfering RNAs. In the cell, NoV B2 binds to pre-Dicer substrate RNA and RNA-induced silencing complex (RISC)-processed RNAs and inhibits the Dicer cleavage reaction and, potentially, one or more post-Dicer activities. In vitro, NoV B2 inhibits Dicer-mediated RNA cleavage in the absence of any other host factors and specifically binds double-stranded RNAs corresponding in structure to Dicer substrates and products. Its abilities to bind to Dicer precursor and post-Dicer RISC-processed RNAs suggest a mechanism of inhibition that is unique among known viral inhibitors of RNAi.</jats:p> A Virus-Encoded Inhibitor That Blocks RNA Interference in Mammalian Cells Journal of Virology |
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| title |
A Virus-Encoded Inhibitor That Blocks RNA Interference in Mammalian Cells |
| title_unstemmed |
A Virus-Encoded Inhibitor That Blocks RNA Interference in Mammalian Cells |
| title_full |
A Virus-Encoded Inhibitor That Blocks RNA Interference in Mammalian Cells |
| title_fullStr |
A Virus-Encoded Inhibitor That Blocks RNA Interference in Mammalian Cells |
| title_full_unstemmed |
A Virus-Encoded Inhibitor That Blocks RNA Interference in Mammalian Cells |
| title_short |
A Virus-Encoded Inhibitor That Blocks RNA Interference in Mammalian Cells |
| title_sort |
a virus-encoded inhibitor that blocks rna interference in mammalian cells |
| topic |
Virology Insect Science Immunology Microbiology |
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http://dx.doi.org/10.1128/jvi.79.12.7371-7379.2005 |
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2005 |
| physical |
7371-7379 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>Nodamura virus (NoV) is a small RNA virus that is infectious for insect and mammalian hosts. We have developed a highly sensitive assay of RNA interference (RNAi) in mammalian cells that shows that the NoV B2 protein functions as an inhibitor of RNAi triggered by either short hairpin RNAs or small interfering RNAs. In the cell, NoV B2 binds to pre-Dicer substrate RNA and RNA-induced silencing complex (RISC)-processed RNAs and inhibits the Dicer cleavage reaction and, potentially, one or more post-Dicer activities. In vitro, NoV B2 inhibits Dicer-mediated RNA cleavage in the absence of any other host factors and specifically binds double-stranded RNAs corresponding in structure to Dicer substrates and products. Its abilities to bind to Dicer precursor and post-Dicer RISC-processed RNAs suggest a mechanism of inhibition that is unique among known viral inhibitors of RNAi.</jats:p> |
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| author | Sullivan, Christopher S., Ganem, Don |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p>Nodamura virus (NoV) is a small RNA virus that is infectious for insect and mammalian hosts. We have developed a highly sensitive assay of RNA interference (RNAi) in mammalian cells that shows that the NoV B2 protein functions as an inhibitor of RNAi triggered by either short hairpin RNAs or small interfering RNAs. In the cell, NoV B2 binds to pre-Dicer substrate RNA and RNA-induced silencing complex (RISC)-processed RNAs and inhibits the Dicer cleavage reaction and, potentially, one or more post-Dicer activities. In vitro, NoV B2 inhibits Dicer-mediated RNA cleavage in the absence of any other host factors and specifically binds double-stranded RNAs corresponding in structure to Dicer substrates and products. Its abilities to bind to Dicer precursor and post-Dicer RISC-processed RNAs suggest a mechanism of inhibition that is unique among known viral inhibitors of RNAi.</jats:p> |
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| physical | 7371-7379 |
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| spelling | Sullivan, Christopher S. Ganem, Don 0022-538X 1098-5514 American Society for Microbiology Virology Insect Science Immunology Microbiology http://dx.doi.org/10.1128/jvi.79.12.7371-7379.2005 <jats:title>ABSTRACT</jats:title> <jats:p>Nodamura virus (NoV) is a small RNA virus that is infectious for insect and mammalian hosts. We have developed a highly sensitive assay of RNA interference (RNAi) in mammalian cells that shows that the NoV B2 protein functions as an inhibitor of RNAi triggered by either short hairpin RNAs or small interfering RNAs. In the cell, NoV B2 binds to pre-Dicer substrate RNA and RNA-induced silencing complex (RISC)-processed RNAs and inhibits the Dicer cleavage reaction and, potentially, one or more post-Dicer activities. In vitro, NoV B2 inhibits Dicer-mediated RNA cleavage in the absence of any other host factors and specifically binds double-stranded RNAs corresponding in structure to Dicer substrates and products. Its abilities to bind to Dicer precursor and post-Dicer RISC-processed RNAs suggest a mechanism of inhibition that is unique among known viral inhibitors of RNAi.</jats:p> A Virus-Encoded Inhibitor That Blocks RNA Interference in Mammalian Cells Journal of Virology |
| spellingShingle | Sullivan, Christopher S., Ganem, Don, Journal of Virology, A Virus-Encoded Inhibitor That Blocks RNA Interference in Mammalian Cells, Virology, Insect Science, Immunology, Microbiology |
| title | A Virus-Encoded Inhibitor That Blocks RNA Interference in Mammalian Cells |
| title_full | A Virus-Encoded Inhibitor That Blocks RNA Interference in Mammalian Cells |
| title_fullStr | A Virus-Encoded Inhibitor That Blocks RNA Interference in Mammalian Cells |
| title_full_unstemmed | A Virus-Encoded Inhibitor That Blocks RNA Interference in Mammalian Cells |
| title_short | A Virus-Encoded Inhibitor That Blocks RNA Interference in Mammalian Cells |
| title_sort | a virus-encoded inhibitor that blocks rna interference in mammalian cells |
| title_unstemmed | A Virus-Encoded Inhibitor That Blocks RNA Interference in Mammalian Cells |
| topic | Virology, Insect Science, Immunology, Microbiology |
| url | http://dx.doi.org/10.1128/jvi.79.12.7371-7379.2005 |