Eintrag weiter verarbeiten
RIII/Sa Mice with a High Incidence of Mammary Tumors Express Two Exogenous Strains and One Potential Endogenous Strain of Mouse Mammary Tumor Virus
Gespeichert in:
| Zeitschriftentitel: | Journal of Virology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Journal of Virology, 78, 2004, 2, S. 1055-1062 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Sarkar, Nurul H. Golovkina, Tatyana Uz-Zaman, Taher Sarkar, Nurul H. Golovkina, Tatyana Uz-Zaman, Taher |
|---|---|
| author |
Sarkar, Nurul H. Golovkina, Tatyana Uz-Zaman, Taher |
| spellingShingle |
Sarkar, Nurul H. Golovkina, Tatyana Uz-Zaman, Taher Journal of Virology RIII/Sa Mice with a High Incidence of Mammary Tumors Express Two Exogenous Strains and One Potential Endogenous Strain of Mouse Mammary Tumor Virus Virology Insect Science Immunology Microbiology |
| author_sort |
sarkar, nurul h. |
| spelling |
Sarkar, Nurul H. Golovkina, Tatyana Uz-Zaman, Taher 0022-538X 1098-5514 American Society for Microbiology Virology Insect Science Immunology Microbiology http://dx.doi.org/10.1128/jvi.78.2.1055-1062.2004 <jats:title>ABSTRACT</jats:title> <jats:p> The inbred mouse strain RIII has long been known for shedding large amounts of mouse mammary tumor virus (MMTV) particles in milk and for the development of hormone-dependent early mammary tumors at a very high incidence (>90%). We have established one RIII subline (RIII/Sa) that shows a pattern of virus expression and tumor incidence similar to that in RIII mice. In the present study, we analyzed the milk and mammary tumors of RIII/Sa mice for virus characterization by reverse transcriptase PCR (RT-PCR) cloning and sequencing of the open reading frame (ORF) of the MMTV long terminal repeats (LTRs). Our results show that these mice express a mixture of at least three different MMTV strains, two of which, designated here as RIII/Sa MMTV-1 and RIII/Sa MMTV-2, are exogenous. The third virus, RIII/Sa MMTV-3, appears to carry the signature of an endogenous provirus, <jats:italic>Mtv</jats:italic> - <jats:italic>17</jats:italic> . Similar studies done with the milk and mammary glands of another subline, RIIIS/J, revealed that they do not express MMTV in their milk. The RIII/Sa and RIIIS/J mice also exhibited differences in their endogenous proviral contents. Twelve spontaneously developed mammary tumors of RIII/Sa mice were examined for possible <jats:italic>Wnt</jats:italic> - <jats:italic>1</jats:italic> and/or <jats:italic>int</jats:italic> - <jats:italic>2</jats:italic> / <jats:italic>Fgf</jats:italic> 3 mutations that are usually found to occur in most mouse mammary tumors as a consequence of MMTV proviral integration. This work led to the isolation of one MMTV- <jats:italic>Wnt</jats:italic> - <jats:italic>1</jats:italic> junction fragment and one MMTV- <jats:italic>int</jats:italic> - <jats:italic>2</jats:italic> / <jats:italic>Fgf</jats:italic> 3 junction fragment from 2 of the 12 tumors. Further analyses showed that both junction fragments contained the RIII/Sa MMTV-2-specific LTR ORF, indicating that this virus was involved in the development of both tumors. Whether RIII/Sa MMTV-1 and/or RIII/Sa MMTV-3 plays any role in mammary tumor development in RIII/Sa mice remains to be established. Overall, the present study demonstrates, to our surprise, that (i) RIII/Sa mice express, unlike other native mouse strains, three strains of MMTVs; and (ii) the virions are completely different from the virus expressed by another subline of RIII mice, the BR6 mice. </jats:p> RIII/Sa Mice with a High Incidence of Mammary Tumors Express Two Exogenous Strains and One Potential Endogenous Strain of Mouse Mammary Tumor Virus Journal of Virology |
| doi_str_mv |
10.1128/jvi.78.2.1055-1062.2004 |
| facet_avail |
Online Free |
| finc_class_facet |
Medizin Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qdmkuNzguMi4xMDU1LTEwNjIuMjAwNA |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qdmkuNzguMi4xMDU1LTEwNjIuMjAwNA |
| institution |
DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 |
| imprint |
American Society for Microbiology, 2004 |
| imprint_str_mv |
American Society for Microbiology, 2004 |
| issn |
0022-538X 1098-5514 |
| issn_str_mv |
0022-538X 1098-5514 |
| language |
English |
| mega_collection |
American Society for Microbiology (CrossRef) |
| match_str |
sarkar2004riiisamicewithahighincidenceofmammarytumorsexpresstwoexogenousstrainsandonepotentialendogenousstrainofmousemammarytumorvirus |
| publishDateSort |
2004 |
| publisher |
American Society for Microbiology |
| recordtype |
ai |
| record_format |
ai |
| series |
Journal of Virology |
| source_id |
49 |
| title |
RIII/Sa Mice with a High Incidence of Mammary Tumors Express Two Exogenous Strains and One Potential Endogenous Strain of Mouse Mammary Tumor Virus |
| title_unstemmed |
RIII/Sa Mice with a High Incidence of Mammary Tumors Express Two Exogenous Strains and One Potential Endogenous Strain of Mouse Mammary Tumor Virus |
| title_full |
RIII/Sa Mice with a High Incidence of Mammary Tumors Express Two Exogenous Strains and One Potential Endogenous Strain of Mouse Mammary Tumor Virus |
| title_fullStr |
RIII/Sa Mice with a High Incidence of Mammary Tumors Express Two Exogenous Strains and One Potential Endogenous Strain of Mouse Mammary Tumor Virus |
| title_full_unstemmed |
RIII/Sa Mice with a High Incidence of Mammary Tumors Express Two Exogenous Strains and One Potential Endogenous Strain of Mouse Mammary Tumor Virus |
| title_short |
RIII/Sa Mice with a High Incidence of Mammary Tumors Express Two Exogenous Strains and One Potential Endogenous Strain of Mouse Mammary Tumor Virus |
| title_sort |
riii/sa mice with a high incidence of mammary tumors express two exogenous strains and one potential endogenous strain of mouse mammary tumor virus |
| topic |
Virology Insect Science Immunology Microbiology |
| url |
http://dx.doi.org/10.1128/jvi.78.2.1055-1062.2004 |
| publishDate |
2004 |
| physical |
1055-1062 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
The inbred mouse strain RIII has long been known for shedding large amounts of mouse mammary tumor virus (MMTV) particles in milk and for the development of hormone-dependent early mammary tumors at a very high incidence (>90%). We have established one RIII subline (RIII/Sa) that shows a pattern of virus expression and tumor incidence similar to that in RIII mice. In the present study, we analyzed the milk and mammary tumors of RIII/Sa mice for virus characterization by reverse transcriptase PCR (RT-PCR) cloning and sequencing of the open reading frame (ORF) of the MMTV long terminal repeats (LTRs). Our results show that these mice express a mixture of at least three different MMTV strains, two of which, designated here as RIII/Sa MMTV-1 and RIII/Sa MMTV-2, are exogenous. The third virus, RIII/Sa MMTV-3, appears to carry the signature of an endogenous provirus,
<jats:italic>Mtv</jats:italic>
-
<jats:italic>17</jats:italic>
. Similar studies done with the milk and mammary glands of another subline, RIIIS/J, revealed that they do not express MMTV in their milk. The RIII/Sa and RIIIS/J mice also exhibited differences in their endogenous proviral contents. Twelve spontaneously developed mammary tumors of RIII/Sa mice were examined for possible
<jats:italic>Wnt</jats:italic>
-
<jats:italic>1</jats:italic>
and/or
<jats:italic>int</jats:italic>
-
<jats:italic>2</jats:italic>
/
<jats:italic>Fgf</jats:italic>
3 mutations that are usually found to occur in most mouse mammary tumors as a consequence of MMTV proviral integration. This work led to the isolation of one MMTV-
<jats:italic>Wnt</jats:italic>
-
<jats:italic>1</jats:italic>
junction fragment and one MMTV-
<jats:italic>int</jats:italic>
-
<jats:italic>2</jats:italic>
/
<jats:italic>Fgf</jats:italic>
3 junction fragment from 2 of the 12 tumors. Further analyses showed that both junction fragments contained the RIII/Sa MMTV-2-specific LTR ORF, indicating that this virus was involved in the development of both tumors. Whether RIII/Sa MMTV-1 and/or RIII/Sa MMTV-3 plays any role in mammary tumor development in RIII/Sa mice remains to be established. Overall, the present study demonstrates, to our surprise, that (i) RIII/Sa mice express, unlike other native mouse strains, three strains of MMTVs; and (ii) the virions are completely different from the virus expressed by another subline of RIII mice, the BR6 mice.
</jats:p> |
| container_issue |
2 |
| container_start_page |
1055 |
| container_title |
Journal of Virology |
| container_volume |
78 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792333579375607819 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T14:14:59.331Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=RIII%2FSa+Mice+with+a+High+Incidence+of+Mammary+Tumors+Express+Two+Exogenous+Strains+and+One+Potential+Endogenous+Strain+of+Mouse+Mammary+Tumor+Virus&rft.date=2004-01-15&genre=article&issn=1098-5514&volume=78&issue=2&spage=1055&epage=1062&pages=1055-1062&jtitle=Journal+of+Virology&atitle=RIII%2FSa+Mice+with+a+High+Incidence+of+Mammary+Tumors+Express+Two+Exogenous+Strains+and+One+Potential+Endogenous+Strain+of+Mouse+Mammary+Tumor+Virus&aulast=Uz-Zaman&aufirst=Taher&rft_id=info%3Adoi%2F10.1128%2Fjvi.78.2.1055-1062.2004&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792333579375607819 |
| author | Sarkar, Nurul H., Golovkina, Tatyana, Uz-Zaman, Taher |
| author_facet | Sarkar, Nurul H., Golovkina, Tatyana, Uz-Zaman, Taher, Sarkar, Nurul H., Golovkina, Tatyana, Uz-Zaman, Taher |
| author_sort | sarkar, nurul h. |
| container_issue | 2 |
| container_start_page | 1055 |
| container_title | Journal of Virology |
| container_volume | 78 |
| description | <jats:title>ABSTRACT</jats:title> <jats:p> The inbred mouse strain RIII has long been known for shedding large amounts of mouse mammary tumor virus (MMTV) particles in milk and for the development of hormone-dependent early mammary tumors at a very high incidence (>90%). We have established one RIII subline (RIII/Sa) that shows a pattern of virus expression and tumor incidence similar to that in RIII mice. In the present study, we analyzed the milk and mammary tumors of RIII/Sa mice for virus characterization by reverse transcriptase PCR (RT-PCR) cloning and sequencing of the open reading frame (ORF) of the MMTV long terminal repeats (LTRs). Our results show that these mice express a mixture of at least three different MMTV strains, two of which, designated here as RIII/Sa MMTV-1 and RIII/Sa MMTV-2, are exogenous. The third virus, RIII/Sa MMTV-3, appears to carry the signature of an endogenous provirus, <jats:italic>Mtv</jats:italic> - <jats:italic>17</jats:italic> . Similar studies done with the milk and mammary glands of another subline, RIIIS/J, revealed that they do not express MMTV in their milk. The RIII/Sa and RIIIS/J mice also exhibited differences in their endogenous proviral contents. Twelve spontaneously developed mammary tumors of RIII/Sa mice were examined for possible <jats:italic>Wnt</jats:italic> - <jats:italic>1</jats:italic> and/or <jats:italic>int</jats:italic> - <jats:italic>2</jats:italic> / <jats:italic>Fgf</jats:italic> 3 mutations that are usually found to occur in most mouse mammary tumors as a consequence of MMTV proviral integration. This work led to the isolation of one MMTV- <jats:italic>Wnt</jats:italic> - <jats:italic>1</jats:italic> junction fragment and one MMTV- <jats:italic>int</jats:italic> - <jats:italic>2</jats:italic> / <jats:italic>Fgf</jats:italic> 3 junction fragment from 2 of the 12 tumors. Further analyses showed that both junction fragments contained the RIII/Sa MMTV-2-specific LTR ORF, indicating that this virus was involved in the development of both tumors. Whether RIII/Sa MMTV-1 and/or RIII/Sa MMTV-3 plays any role in mammary tumor development in RIII/Sa mice remains to be established. Overall, the present study demonstrates, to our surprise, that (i) RIII/Sa mice express, unlike other native mouse strains, three strains of MMTVs; and (ii) the virions are completely different from the virus expressed by another subline of RIII mice, the BR6 mice. </jats:p> |
| doi_str_mv | 10.1128/jvi.78.2.1055-1062.2004 |
| facet_avail | Online, Free |
| finc_class_facet | Medizin, Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qdmkuNzguMi4xMDU1LTEwNjIuMjAwNA |
| imprint | American Society for Microbiology, 2004 |
| imprint_str_mv | American Society for Microbiology, 2004 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
| issn | 0022-538X, 1098-5514 |
| issn_str_mv | 0022-538X, 1098-5514 |
| language | English |
| last_indexed | 2024-03-01T14:14:59.331Z |
| match_str | sarkar2004riiisamicewithahighincidenceofmammarytumorsexpresstwoexogenousstrainsandonepotentialendogenousstrainofmousemammarytumorvirus |
| mega_collection | American Society for Microbiology (CrossRef) |
| physical | 1055-1062 |
| publishDate | 2004 |
| publishDateSort | 2004 |
| publisher | American Society for Microbiology |
| record_format | ai |
| recordtype | ai |
| series | Journal of Virology |
| source_id | 49 |
| spelling | Sarkar, Nurul H. Golovkina, Tatyana Uz-Zaman, Taher 0022-538X 1098-5514 American Society for Microbiology Virology Insect Science Immunology Microbiology http://dx.doi.org/10.1128/jvi.78.2.1055-1062.2004 <jats:title>ABSTRACT</jats:title> <jats:p> The inbred mouse strain RIII has long been known for shedding large amounts of mouse mammary tumor virus (MMTV) particles in milk and for the development of hormone-dependent early mammary tumors at a very high incidence (>90%). We have established one RIII subline (RIII/Sa) that shows a pattern of virus expression and tumor incidence similar to that in RIII mice. In the present study, we analyzed the milk and mammary tumors of RIII/Sa mice for virus characterization by reverse transcriptase PCR (RT-PCR) cloning and sequencing of the open reading frame (ORF) of the MMTV long terminal repeats (LTRs). Our results show that these mice express a mixture of at least three different MMTV strains, two of which, designated here as RIII/Sa MMTV-1 and RIII/Sa MMTV-2, are exogenous. The third virus, RIII/Sa MMTV-3, appears to carry the signature of an endogenous provirus, <jats:italic>Mtv</jats:italic> - <jats:italic>17</jats:italic> . Similar studies done with the milk and mammary glands of another subline, RIIIS/J, revealed that they do not express MMTV in their milk. The RIII/Sa and RIIIS/J mice also exhibited differences in their endogenous proviral contents. Twelve spontaneously developed mammary tumors of RIII/Sa mice were examined for possible <jats:italic>Wnt</jats:italic> - <jats:italic>1</jats:italic> and/or <jats:italic>int</jats:italic> - <jats:italic>2</jats:italic> / <jats:italic>Fgf</jats:italic> 3 mutations that are usually found to occur in most mouse mammary tumors as a consequence of MMTV proviral integration. This work led to the isolation of one MMTV- <jats:italic>Wnt</jats:italic> - <jats:italic>1</jats:italic> junction fragment and one MMTV- <jats:italic>int</jats:italic> - <jats:italic>2</jats:italic> / <jats:italic>Fgf</jats:italic> 3 junction fragment from 2 of the 12 tumors. Further analyses showed that both junction fragments contained the RIII/Sa MMTV-2-specific LTR ORF, indicating that this virus was involved in the development of both tumors. Whether RIII/Sa MMTV-1 and/or RIII/Sa MMTV-3 plays any role in mammary tumor development in RIII/Sa mice remains to be established. Overall, the present study demonstrates, to our surprise, that (i) RIII/Sa mice express, unlike other native mouse strains, three strains of MMTVs; and (ii) the virions are completely different from the virus expressed by another subline of RIII mice, the BR6 mice. </jats:p> RIII/Sa Mice with a High Incidence of Mammary Tumors Express Two Exogenous Strains and One Potential Endogenous Strain of Mouse Mammary Tumor Virus Journal of Virology |
| spellingShingle | Sarkar, Nurul H., Golovkina, Tatyana, Uz-Zaman, Taher, Journal of Virology, RIII/Sa Mice with a High Incidence of Mammary Tumors Express Two Exogenous Strains and One Potential Endogenous Strain of Mouse Mammary Tumor Virus, Virology, Insect Science, Immunology, Microbiology |
| title | RIII/Sa Mice with a High Incidence of Mammary Tumors Express Two Exogenous Strains and One Potential Endogenous Strain of Mouse Mammary Tumor Virus |
| title_full | RIII/Sa Mice with a High Incidence of Mammary Tumors Express Two Exogenous Strains and One Potential Endogenous Strain of Mouse Mammary Tumor Virus |
| title_fullStr | RIII/Sa Mice with a High Incidence of Mammary Tumors Express Two Exogenous Strains and One Potential Endogenous Strain of Mouse Mammary Tumor Virus |
| title_full_unstemmed | RIII/Sa Mice with a High Incidence of Mammary Tumors Express Two Exogenous Strains and One Potential Endogenous Strain of Mouse Mammary Tumor Virus |
| title_short | RIII/Sa Mice with a High Incidence of Mammary Tumors Express Two Exogenous Strains and One Potential Endogenous Strain of Mouse Mammary Tumor Virus |
| title_sort | riii/sa mice with a high incidence of mammary tumors express two exogenous strains and one potential endogenous strain of mouse mammary tumor virus |
| title_unstemmed | RIII/Sa Mice with a High Incidence of Mammary Tumors Express Two Exogenous Strains and One Potential Endogenous Strain of Mouse Mammary Tumor Virus |
| topic | Virology, Insect Science, Immunology, Microbiology |
| url | http://dx.doi.org/10.1128/jvi.78.2.1055-1062.2004 |