Eintrag weiter verarbeiten
Polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (BLV) gp51 binding to recombinant BLV receptor BLVRcp1
Gespeichert in:
| Zeitschriftentitel: | Journal of Virology |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | Journal of Virology, 71, 1997, 4, S. 3263-3267 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Orlik, O Ban, J Hlavaty, J Altaner, C Kettmann, R Portetelle, D Splitter, G A Orlik, O Ban, J Hlavaty, J Altaner, C Kettmann, R Portetelle, D Splitter, G A |
|---|---|
| author |
Orlik, O Ban, J Hlavaty, J Altaner, C Kettmann, R Portetelle, D Splitter, G A |
| spellingShingle |
Orlik, O Ban, J Hlavaty, J Altaner, C Kettmann, R Portetelle, D Splitter, G A Journal of Virology Polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (BLV) gp51 binding to recombinant BLV receptor BLVRcp1 Virology Insect Science Immunology Microbiology |
| author_sort |
orlik, o |
| spelling |
Orlik, O Ban, J Hlavaty, J Altaner, C Kettmann, R Portetelle, D Splitter, G A 0022-538X 1098-5514 American Society for Microbiology Virology Insect Science Immunology Microbiology http://dx.doi.org/10.1128/jvi.71.4.3263-3267.1997 <jats:p>Bovine leukemia virus (BLV), a transactivating lymphotropic retrovirus, is the etiologic agent of enzootic lymphosarcoma or leukemia in cattle. Sera from BLV-infected animals possess high BLV-neutralizing antibody titres. The availability of the recombinant BLV receptor candidate, BLVRcp1, allowed us to determine a mechanism of virus neutralization by polyclonal sera and monoclonal antibodies (MAbs). Bovine sera from animals naturally infected with BLV blocked gp51 binding to recombinant BLVRcp1. In contrast, virus-neutralizing MAbs specific for gp51 F, G, and H epitopes did not prevent gp51-receptor attachment. Furthermore, gp51 neutralization epitopes F, G, and H were accessible to antibodies following gp51 attachment to BLVRcp1. This finding implies that virus neutralization by MAbs to defined BLV gp51 epitopes can occur subsequent to virus engagement of the receptor while polyclonal sera can specifically block virus attachment to the receptor. In conclusion, these data suggest that cell infection by BLV is a multistep process requiring receptor binding (inhibited by polyclonal sera) followed by a second, postbinding event(s) at the cell membrane (inhibited by anti-gp51 MAbs).</jats:p> Polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (BLV) gp51 binding to recombinant BLV receptor BLVRcp1 Journal of Virology |
| doi_str_mv |
10.1128/jvi.71.4.3263-3267.1997 |
| facet_avail |
Online Free |
| finc_class_facet |
Medizin Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qdmkuNzEuNC4zMjYzLTMyNjcuMTk5Nw |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qdmkuNzEuNC4zMjYzLTMyNjcuMTk5Nw |
| institution |
DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-D161 DE-Zwi2 DE-Gla1 DE-Zi4 |
| imprint |
American Society for Microbiology, 1997 |
| imprint_str_mv |
American Society for Microbiology, 1997 |
| issn |
0022-538X 1098-5514 |
| issn_str_mv |
0022-538X 1098-5514 |
| language |
English |
| mega_collection |
American Society for Microbiology (CrossRef) |
| match_str |
orlik1997polyclonalbovineserabutnotvirusneutralizingmonoclonalantibodiesblockbovineleukemiavirusblvgp51bindingtorecombinantblvreceptorblvrcp1 |
| publishDateSort |
1997 |
| publisher |
American Society for Microbiology |
| recordtype |
ai |
| record_format |
ai |
| series |
Journal of Virology |
| source_id |
49 |
| title |
Polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (BLV) gp51 binding to recombinant BLV receptor BLVRcp1 |
| title_unstemmed |
Polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (BLV) gp51 binding to recombinant BLV receptor BLVRcp1 |
| title_full |
Polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (BLV) gp51 binding to recombinant BLV receptor BLVRcp1 |
| title_fullStr |
Polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (BLV) gp51 binding to recombinant BLV receptor BLVRcp1 |
| title_full_unstemmed |
Polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (BLV) gp51 binding to recombinant BLV receptor BLVRcp1 |
| title_short |
Polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (BLV) gp51 binding to recombinant BLV receptor BLVRcp1 |
| title_sort |
polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (blv) gp51 binding to recombinant blv receptor blvrcp1 |
| topic |
Virology Insect Science Immunology Microbiology |
| url |
http://dx.doi.org/10.1128/jvi.71.4.3263-3267.1997 |
| publishDate |
1997 |
| physical |
3263-3267 |
| description |
<jats:p>Bovine leukemia virus (BLV), a transactivating lymphotropic retrovirus, is the etiologic agent of enzootic lymphosarcoma or leukemia in cattle. Sera from BLV-infected animals possess high BLV-neutralizing antibody titres. The availability of the recombinant BLV receptor candidate, BLVRcp1, allowed us to determine a mechanism of virus neutralization by polyclonal sera and monoclonal antibodies (MAbs). Bovine sera from animals naturally infected with BLV blocked gp51 binding to recombinant BLVRcp1. In contrast, virus-neutralizing MAbs specific for gp51 F, G, and H epitopes did not prevent gp51-receptor attachment. Furthermore, gp51 neutralization epitopes F, G, and H were accessible to antibodies following gp51 attachment to BLVRcp1. This finding implies that virus neutralization by MAbs to defined BLV gp51 epitopes can occur subsequent to virus engagement of the receptor while polyclonal sera can specifically block virus attachment to the receptor. In conclusion, these data suggest that cell infection by BLV is a multistep process requiring receptor binding (inhibited by polyclonal sera) followed by a second, postbinding event(s) at the cell membrane (inhibited by anti-gp51 MAbs).</jats:p> |
| container_issue |
4 |
| container_start_page |
3263 |
| container_title |
Journal of Virology |
| container_volume |
71 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792332491846057996 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T13:57:15.128Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Polyclonal+bovine+sera+but+not+virus-neutralizing+monoclonal+antibodies+block+bovine+leukemia+virus+%28BLV%29+gp51+binding+to+recombinant+BLV+receptor+BLVRcp1&rft.date=1997-04-01&genre=article&issn=1098-5514&volume=71&issue=4&spage=3263&epage=3267&pages=3263-3267&jtitle=Journal+of+Virology&atitle=Polyclonal+bovine+sera+but+not+virus-neutralizing+monoclonal+antibodies+block+bovine+leukemia+virus+%28BLV%29+gp51+binding+to+recombinant+BLV+receptor+BLVRcp1&aulast=Splitter&aufirst=G+A&rft_id=info%3Adoi%2F10.1128%2Fjvi.71.4.3263-3267.1997&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792332491846057996 |
| author | Orlik, O, Ban, J, Hlavaty, J, Altaner, C, Kettmann, R, Portetelle, D, Splitter, G A |
| author_facet | Orlik, O, Ban, J, Hlavaty, J, Altaner, C, Kettmann, R, Portetelle, D, Splitter, G A, Orlik, O, Ban, J, Hlavaty, J, Altaner, C, Kettmann, R, Portetelle, D, Splitter, G A |
| author_sort | orlik, o |
| container_issue | 4 |
| container_start_page | 3263 |
| container_title | Journal of Virology |
| container_volume | 71 |
| description | <jats:p>Bovine leukemia virus (BLV), a transactivating lymphotropic retrovirus, is the etiologic agent of enzootic lymphosarcoma or leukemia in cattle. Sera from BLV-infected animals possess high BLV-neutralizing antibody titres. The availability of the recombinant BLV receptor candidate, BLVRcp1, allowed us to determine a mechanism of virus neutralization by polyclonal sera and monoclonal antibodies (MAbs). Bovine sera from animals naturally infected with BLV blocked gp51 binding to recombinant BLVRcp1. In contrast, virus-neutralizing MAbs specific for gp51 F, G, and H epitopes did not prevent gp51-receptor attachment. Furthermore, gp51 neutralization epitopes F, G, and H were accessible to antibodies following gp51 attachment to BLVRcp1. This finding implies that virus neutralization by MAbs to defined BLV gp51 epitopes can occur subsequent to virus engagement of the receptor while polyclonal sera can specifically block virus attachment to the receptor. In conclusion, these data suggest that cell infection by BLV is a multistep process requiring receptor binding (inhibited by polyclonal sera) followed by a second, postbinding event(s) at the cell membrane (inhibited by anti-gp51 MAbs).</jats:p> |
| doi_str_mv | 10.1128/jvi.71.4.3263-3267.1997 |
| facet_avail | Online, Free |
| finc_class_facet | Medizin, Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qdmkuNzEuNC4zMjYzLTMyNjcuMTk5Nw |
| imprint | American Society for Microbiology, 1997 |
| imprint_str_mv | American Society for Microbiology, 1997 |
| institution | DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2, DE-Gla1, DE-Zi4 |
| issn | 0022-538X, 1098-5514 |
| issn_str_mv | 0022-538X, 1098-5514 |
| language | English |
| last_indexed | 2024-03-01T13:57:15.128Z |
| match_str | orlik1997polyclonalbovineserabutnotvirusneutralizingmonoclonalantibodiesblockbovineleukemiavirusblvgp51bindingtorecombinantblvreceptorblvrcp1 |
| mega_collection | American Society for Microbiology (CrossRef) |
| physical | 3263-3267 |
| publishDate | 1997 |
| publishDateSort | 1997 |
| publisher | American Society for Microbiology |
| record_format | ai |
| recordtype | ai |
| series | Journal of Virology |
| source_id | 49 |
| spelling | Orlik, O Ban, J Hlavaty, J Altaner, C Kettmann, R Portetelle, D Splitter, G A 0022-538X 1098-5514 American Society for Microbiology Virology Insect Science Immunology Microbiology http://dx.doi.org/10.1128/jvi.71.4.3263-3267.1997 <jats:p>Bovine leukemia virus (BLV), a transactivating lymphotropic retrovirus, is the etiologic agent of enzootic lymphosarcoma or leukemia in cattle. Sera from BLV-infected animals possess high BLV-neutralizing antibody titres. The availability of the recombinant BLV receptor candidate, BLVRcp1, allowed us to determine a mechanism of virus neutralization by polyclonal sera and monoclonal antibodies (MAbs). Bovine sera from animals naturally infected with BLV blocked gp51 binding to recombinant BLVRcp1. In contrast, virus-neutralizing MAbs specific for gp51 F, G, and H epitopes did not prevent gp51-receptor attachment. Furthermore, gp51 neutralization epitopes F, G, and H were accessible to antibodies following gp51 attachment to BLVRcp1. This finding implies that virus neutralization by MAbs to defined BLV gp51 epitopes can occur subsequent to virus engagement of the receptor while polyclonal sera can specifically block virus attachment to the receptor. In conclusion, these data suggest that cell infection by BLV is a multistep process requiring receptor binding (inhibited by polyclonal sera) followed by a second, postbinding event(s) at the cell membrane (inhibited by anti-gp51 MAbs).</jats:p> Polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (BLV) gp51 binding to recombinant BLV receptor BLVRcp1 Journal of Virology |
| spellingShingle | Orlik, O, Ban, J, Hlavaty, J, Altaner, C, Kettmann, R, Portetelle, D, Splitter, G A, Journal of Virology, Polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (BLV) gp51 binding to recombinant BLV receptor BLVRcp1, Virology, Insect Science, Immunology, Microbiology |
| title | Polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (BLV) gp51 binding to recombinant BLV receptor BLVRcp1 |
| title_full | Polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (BLV) gp51 binding to recombinant BLV receptor BLVRcp1 |
| title_fullStr | Polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (BLV) gp51 binding to recombinant BLV receptor BLVRcp1 |
| title_full_unstemmed | Polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (BLV) gp51 binding to recombinant BLV receptor BLVRcp1 |
| title_short | Polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (BLV) gp51 binding to recombinant BLV receptor BLVRcp1 |
| title_sort | polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (blv) gp51 binding to recombinant blv receptor blvrcp1 |
| title_unstemmed | Polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (BLV) gp51 binding to recombinant BLV receptor BLVRcp1 |
| topic | Virology, Insect Science, Immunology, Microbiology |
| url | http://dx.doi.org/10.1128/jvi.71.4.3263-3267.1997 |