Interaction between echovirus 7 and its receptor, decay-accelerating factor (CD55): evidence for a secondary cellular factor in A-particle formation

Gespeichert in:

Bibliographische Detailangaben
Zeitschriftentitel:	Journal of Virology
Personen und Körperschaften:	Powell, R M, Ward, T, Evans, D J, Almond, J W
In:	Journal of Virology, 71, 1997, 12, S. 9306-9312
Format:	E-Article
Sprache:	Englisch
veröffentlicht:	American Society for Microbiology
Schlagwörter:	Virology Insect Science Immunology Microbiology

Details
Zusammenfassung:	<jats:p>Soluble forms of decay-accelerating factor (DAF) (CD55), the receptor for echovirus 7, were synthesized in the yeast Pichia pastoris. Purified recombinant protein containing SCR domains 2, 3, and 4, but lacking the serine/threonine rich region, was shown to block infection of susceptible cells by echovirus 7. In contrast to the situation with poliovirus and its receptor, the neutralization of echovirus 7 by soluble DAF was completely reversible and did not lead to the formation of 135S A-particles. Binding of virus to susceptible cells, by contrast, did lead to the formation of A particles, mainly from virus that had been internalized. The data suggest that a secondary factor(s) may contribute to A-particle formation and uncoating of echovirus 7.</jats:p>
Umfang:	9306-9312
ISSN:	0022-538X 1098-5514
DOI:	10.1128/jvi.71.12.9306-9312.1997