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Detection of a Protein of Avian Leukoviruses in Uninfected Chick Cells by Radioimmunoassay
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| Zeitschriftentitel: | Journal of Virology |
|---|---|
| Personen und Körperschaften: | , |
| In: | Journal of Virology, 13, 1974, 2, S. 340-346 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Chen, J. H. Hanafusa, Hidesaburo Chen, J. H. Hanafusa, Hidesaburo |
|---|---|
| author |
Chen, J. H. Hanafusa, Hidesaburo |
| spellingShingle |
Chen, J. H. Hanafusa, Hidesaburo Journal of Virology Detection of a Protein of Avian Leukoviruses in Uninfected Chick Cells by Radioimmunoassay Virology Insect Science Immunology Microbiology |
| author_sort |
chen, j. h. |
| spelling |
Chen, J. H. Hanafusa, Hidesaburo 0022-538X 1098-5514 American Society for Microbiology Virology Insect Science Immunology Microbiology http://dx.doi.org/10.1128/jvi.13.2.340-346.1974 <jats:p>A radioimmunoassay (RIA) has been used to measure the quantity of protein P27 (mol wt 27,000; group specific-1 [gs-1]) of avian leukoviruses in different types of chicken embryos and tissues of adult chickens. The RIA used was 200- to 300-fold more sensitive than the complement fixation test and was able to detect as little as 0.3 ng of P27. Among six embryos tested, which are negative for gs antigen by the complement fixation test, P27 was undetectable in three embryos, but another three contained about 5 ng of P27 per mg of cell protein by RIA. The amount of P27 in gs antigen-positive cells ranged from 22 to 57 ng per mg of cell protein. P27 was found in liver, lung, ovary, feather pulp, and spleen from adult gs antigen-positive chickens. This protein was undetectable in various tissues from gs antigen-negative chickens.</jats:p> Detection of a Protein of Avian Leukoviruses in Uninfected Chick Cells by Radioimmunoassay Journal of Virology |
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American Society for Microbiology, 1974 |
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American Society for Microbiology, 1974 |
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Journal of Virology |
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| title |
Detection of a Protein of Avian Leukoviruses in Uninfected Chick Cells by Radioimmunoassay |
| title_unstemmed |
Detection of a Protein of Avian Leukoviruses in Uninfected Chick Cells by Radioimmunoassay |
| title_full |
Detection of a Protein of Avian Leukoviruses in Uninfected Chick Cells by Radioimmunoassay |
| title_fullStr |
Detection of a Protein of Avian Leukoviruses in Uninfected Chick Cells by Radioimmunoassay |
| title_full_unstemmed |
Detection of a Protein of Avian Leukoviruses in Uninfected Chick Cells by Radioimmunoassay |
| title_short |
Detection of a Protein of Avian Leukoviruses in Uninfected Chick Cells by Radioimmunoassay |
| title_sort |
detection of a protein of avian leukoviruses in uninfected chick cells by radioimmunoassay |
| topic |
Virology Insect Science Immunology Microbiology |
| url |
http://dx.doi.org/10.1128/jvi.13.2.340-346.1974 |
| publishDate |
1974 |
| physical |
340-346 |
| description |
<jats:p>A radioimmunoassay (RIA) has been used to measure the quantity of protein P27 (mol wt 27,000; group specific-1 [gs-1]) of avian leukoviruses in different types of chicken embryos and tissues of adult chickens. The RIA used was 200- to 300-fold more sensitive than the complement fixation test and was able to detect as little as 0.3 ng of P27. Among six embryos tested, which are negative for gs antigen by the complement fixation test, P27 was undetectable in three embryos, but another three contained about 5 ng of P27 per mg of cell protein by RIA. The amount of P27 in gs antigen-positive cells ranged from 22 to 57 ng per mg of cell protein. P27 was found in liver, lung, ovary, feather pulp, and spleen from adult gs antigen-positive chickens. This protein was undetectable in various tissues from gs antigen-negative chickens.</jats:p> |
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| author | Chen, J. H., Hanafusa, Hidesaburo |
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| description | <jats:p>A radioimmunoassay (RIA) has been used to measure the quantity of protein P27 (mol wt 27,000; group specific-1 [gs-1]) of avian leukoviruses in different types of chicken embryos and tissues of adult chickens. The RIA used was 200- to 300-fold more sensitive than the complement fixation test and was able to detect as little as 0.3 ng of P27. Among six embryos tested, which are negative for gs antigen by the complement fixation test, P27 was undetectable in three embryos, but another three contained about 5 ng of P27 per mg of cell protein by RIA. The amount of P27 in gs antigen-positive cells ranged from 22 to 57 ng per mg of cell protein. P27 was found in liver, lung, ovary, feather pulp, and spleen from adult gs antigen-positive chickens. This protein was undetectable in various tissues from gs antigen-negative chickens.</jats:p> |
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| physical | 340-346 |
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| spelling | Chen, J. H. Hanafusa, Hidesaburo 0022-538X 1098-5514 American Society for Microbiology Virology Insect Science Immunology Microbiology http://dx.doi.org/10.1128/jvi.13.2.340-346.1974 <jats:p>A radioimmunoassay (RIA) has been used to measure the quantity of protein P27 (mol wt 27,000; group specific-1 [gs-1]) of avian leukoviruses in different types of chicken embryos and tissues of adult chickens. The RIA used was 200- to 300-fold more sensitive than the complement fixation test and was able to detect as little as 0.3 ng of P27. Among six embryos tested, which are negative for gs antigen by the complement fixation test, P27 was undetectable in three embryos, but another three contained about 5 ng of P27 per mg of cell protein by RIA. The amount of P27 in gs antigen-positive cells ranged from 22 to 57 ng per mg of cell protein. P27 was found in liver, lung, ovary, feather pulp, and spleen from adult gs antigen-positive chickens. This protein was undetectable in various tissues from gs antigen-negative chickens.</jats:p> Detection of a Protein of Avian Leukoviruses in Uninfected Chick Cells by Radioimmunoassay Journal of Virology |
| spellingShingle | Chen, J. H., Hanafusa, Hidesaburo, Journal of Virology, Detection of a Protein of Avian Leukoviruses in Uninfected Chick Cells by Radioimmunoassay, Virology, Insect Science, Immunology, Microbiology |
| title | Detection of a Protein of Avian Leukoviruses in Uninfected Chick Cells by Radioimmunoassay |
| title_full | Detection of a Protein of Avian Leukoviruses in Uninfected Chick Cells by Radioimmunoassay |
| title_fullStr | Detection of a Protein of Avian Leukoviruses in Uninfected Chick Cells by Radioimmunoassay |
| title_full_unstemmed | Detection of a Protein of Avian Leukoviruses in Uninfected Chick Cells by Radioimmunoassay |
| title_short | Detection of a Protein of Avian Leukoviruses in Uninfected Chick Cells by Radioimmunoassay |
| title_sort | detection of a protein of avian leukoviruses in uninfected chick cells by radioimmunoassay |
| title_unstemmed | Detection of a Protein of Avian Leukoviruses in Uninfected Chick Cells by Radioimmunoassay |
| topic | Virology, Insect Science, Immunology, Microbiology |
| url | http://dx.doi.org/10.1128/jvi.13.2.340-346.1974 |