Eintrag weiter verarbeiten
High-Resolution Analysis of Intrahost Genetic Diversity in Dengue Virus Serotype 1 Infection Identifies Mixed Infections
Gespeichert in:
| Zeitschriftentitel: | Journal of Virology |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , , , , , , |
| In: | Journal of Virology, 86, 2012, 2, S. 835-843 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Thai, Khoa T. D. Henn, Matthew R. Zody, Michael C. Tricou, Vianney Nguyet, Nguyen Minh Charlebois, Patrick Lennon, Niall J. Green, Lisa de Vries, Peter J. Hien, Tran Tinh Farrar, Jeremy van Doorn, H. Rogier de Jong, Menno D. Birren, Bruce W. Holmes, Edward C. Simmons, Cameron P. Thai, Khoa T. D. Henn, Matthew R. Zody, Michael C. Tricou, Vianney Nguyet, Nguyen Minh Charlebois, Patrick Lennon, Niall J. Green, Lisa de Vries, Peter J. Hien, Tran Tinh Farrar, Jeremy van Doorn, H. Rogier de Jong, Menno D. Birren, Bruce W. Holmes, Edward C. Simmons, Cameron P. |
|---|---|
| author |
Thai, Khoa T. D. Henn, Matthew R. Zody, Michael C. Tricou, Vianney Nguyet, Nguyen Minh Charlebois, Patrick Lennon, Niall J. Green, Lisa de Vries, Peter J. Hien, Tran Tinh Farrar, Jeremy van Doorn, H. Rogier de Jong, Menno D. Birren, Bruce W. Holmes, Edward C. Simmons, Cameron P. |
| spellingShingle |
Thai, Khoa T. D. Henn, Matthew R. Zody, Michael C. Tricou, Vianney Nguyet, Nguyen Minh Charlebois, Patrick Lennon, Niall J. Green, Lisa de Vries, Peter J. Hien, Tran Tinh Farrar, Jeremy van Doorn, H. Rogier de Jong, Menno D. Birren, Bruce W. Holmes, Edward C. Simmons, Cameron P. Journal of Virology High-Resolution Analysis of Intrahost Genetic Diversity in Dengue Virus Serotype 1 Infection Identifies Mixed Infections Virology Insect Science Immunology Microbiology |
| author_sort |
thai, khoa t. d. |
| spelling |
Thai, Khoa T. D. Henn, Matthew R. Zody, Michael C. Tricou, Vianney Nguyet, Nguyen Minh Charlebois, Patrick Lennon, Niall J. Green, Lisa de Vries, Peter J. Hien, Tran Tinh Farrar, Jeremy van Doorn, H. Rogier de Jong, Menno D. Birren, Bruce W. Holmes, Edward C. Simmons, Cameron P. 0022-538X 1098-5514 American Society for Microbiology Virology Insect Science Immunology Microbiology http://dx.doi.org/10.1128/jvi.05985-11 <jats:title>ABSTRACT</jats:title> <jats:p> Little is known about the rate at which genetic variation is generated within intrahost populations of dengue virus (DENV) and what implications this diversity has for dengue pathogenesis, disease severity, and host immunity. Previous studies of intrahost DENV variation have used a low frequency of sampling and/or experimental methods that do not fully account for errors generated through amplification and sequencing of viral RNAs. We investigated the extent and pattern of genetic diversity in sequence data in domain III (DIII) of the envelope (E) gene in serial plasma samples ( <jats:italic>n</jats:italic> = 49) taken from 17 patients infected with DENV type 1 (DENV-1), totaling some 8,458 clones. Statistically rigorous approaches were employed to account for artifactual variants resulting from amplification and sequencing, which we suggest have played a major role in previous studies of intrahost genetic variation. Accordingly, nucleotide sequence diversities of viral populations were very low, with conservative estimates of the average levels of genetic diversity ranging from 0 to 0.0013. Despite such sequence conservation, we observed clear evidence for mixed infection, with the presence of multiple phylogenetically distinct lineages present within the same host, while the presence of stop codon mutations in some samples suggests the action of complementation. In contrast to some previous studies we observed no relationship between the extent and pattern of DENV-1 genetic diversity and disease severity, immune status, or level of viremia. </jats:p> High-Resolution Analysis of Intrahost Genetic Diversity in Dengue Virus Serotype 1 Infection Identifies Mixed Infections Journal of Virology |
| doi_str_mv |
10.1128/jvi.05985-11 |
| facet_avail |
Online Free |
| finc_class_facet |
Medizin Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qdmkuMDU5ODUtMTE |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qdmkuMDU5ODUtMTE |
| institution |
DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 |
| imprint |
American Society for Microbiology, 2012 |
| imprint_str_mv |
American Society for Microbiology, 2012 |
| issn |
0022-538X 1098-5514 |
| issn_str_mv |
0022-538X 1098-5514 |
| language |
English |
| mega_collection |
American Society for Microbiology (CrossRef) |
| match_str |
thai2012highresolutionanalysisofintrahostgeneticdiversityindenguevirusserotype1infectionidentifiesmixedinfections |
| publishDateSort |
2012 |
| publisher |
American Society for Microbiology |
| recordtype |
ai |
| record_format |
ai |
| series |
Journal of Virology |
| source_id |
49 |
| title |
High-Resolution Analysis of Intrahost Genetic Diversity in Dengue Virus Serotype 1 Infection Identifies Mixed Infections |
| title_unstemmed |
High-Resolution Analysis of Intrahost Genetic Diversity in Dengue Virus Serotype 1 Infection Identifies Mixed Infections |
| title_full |
High-Resolution Analysis of Intrahost Genetic Diversity in Dengue Virus Serotype 1 Infection Identifies Mixed Infections |
| title_fullStr |
High-Resolution Analysis of Intrahost Genetic Diversity in Dengue Virus Serotype 1 Infection Identifies Mixed Infections |
| title_full_unstemmed |
High-Resolution Analysis of Intrahost Genetic Diversity in Dengue Virus Serotype 1 Infection Identifies Mixed Infections |
| title_short |
High-Resolution Analysis of Intrahost Genetic Diversity in Dengue Virus Serotype 1 Infection Identifies Mixed Infections |
| title_sort |
high-resolution analysis of intrahost genetic diversity in dengue virus serotype 1 infection identifies mixed infections |
| topic |
Virology Insect Science Immunology Microbiology |
| url |
http://dx.doi.org/10.1128/jvi.05985-11 |
| publishDate |
2012 |
| physical |
835-843 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Little is known about the rate at which genetic variation is generated within intrahost populations of dengue virus (DENV) and what implications this diversity has for dengue pathogenesis, disease severity, and host immunity. Previous studies of intrahost DENV variation have used a low frequency of sampling and/or experimental methods that do not fully account for errors generated through amplification and sequencing of viral RNAs. We investigated the extent and pattern of genetic diversity in sequence data in domain III (DIII) of the envelope (E) gene in serial plasma samples (
<jats:italic>n</jats:italic>
= 49) taken from 17 patients infected with DENV type 1 (DENV-1), totaling some 8,458 clones. Statistically rigorous approaches were employed to account for artifactual variants resulting from amplification and sequencing, which we suggest have played a major role in previous studies of intrahost genetic variation. Accordingly, nucleotide sequence diversities of viral populations were very low, with conservative estimates of the average levels of genetic diversity ranging from 0 to 0.0013. Despite such sequence conservation, we observed clear evidence for mixed infection, with the presence of multiple phylogenetically distinct lineages present within the same host, while the presence of stop codon mutations in some samples suggests the action of complementation. In contrast to some previous studies we observed no relationship between the extent and pattern of DENV-1 genetic diversity and disease severity, immune status, or level of viremia.
</jats:p> |
| container_issue |
2 |
| container_start_page |
835 |
| container_title |
Journal of Virology |
| container_volume |
86 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792336027783790596 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T14:53:55.433Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=High-Resolution+Analysis+of+Intrahost+Genetic+Diversity+in+Dengue+Virus+Serotype+1+Infection+Identifies+Mixed+Infections&rft.date=2012-01-15&genre=article&issn=1098-5514&volume=86&issue=2&spage=835&epage=843&pages=835-843&jtitle=Journal+of+Virology&atitle=High-Resolution+Analysis+of+Intrahost+Genetic+Diversity+in+Dengue+Virus+Serotype+1+Infection+Identifies+Mixed+Infections&aulast=Simmons&aufirst=Cameron+P.&rft_id=info%3Adoi%2F10.1128%2Fjvi.05985-11&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792336027783790596 |
| author | Thai, Khoa T. D., Henn, Matthew R., Zody, Michael C., Tricou, Vianney, Nguyet, Nguyen Minh, Charlebois, Patrick, Lennon, Niall J., Green, Lisa, de Vries, Peter J., Hien, Tran Tinh, Farrar, Jeremy, van Doorn, H. Rogier, de Jong, Menno D., Birren, Bruce W., Holmes, Edward C., Simmons, Cameron P. |
| author_facet | Thai, Khoa T. D., Henn, Matthew R., Zody, Michael C., Tricou, Vianney, Nguyet, Nguyen Minh, Charlebois, Patrick, Lennon, Niall J., Green, Lisa, de Vries, Peter J., Hien, Tran Tinh, Farrar, Jeremy, van Doorn, H. Rogier, de Jong, Menno D., Birren, Bruce W., Holmes, Edward C., Simmons, Cameron P., Thai, Khoa T. D., Henn, Matthew R., Zody, Michael C., Tricou, Vianney, Nguyet, Nguyen Minh, Charlebois, Patrick, Lennon, Niall J., Green, Lisa, de Vries, Peter J., Hien, Tran Tinh, Farrar, Jeremy, van Doorn, H. Rogier, de Jong, Menno D., Birren, Bruce W., Holmes, Edward C., Simmons, Cameron P. |
| author_sort | thai, khoa t. d. |
| container_issue | 2 |
| container_start_page | 835 |
| container_title | Journal of Virology |
| container_volume | 86 |
| description | <jats:title>ABSTRACT</jats:title> <jats:p> Little is known about the rate at which genetic variation is generated within intrahost populations of dengue virus (DENV) and what implications this diversity has for dengue pathogenesis, disease severity, and host immunity. Previous studies of intrahost DENV variation have used a low frequency of sampling and/or experimental methods that do not fully account for errors generated through amplification and sequencing of viral RNAs. We investigated the extent and pattern of genetic diversity in sequence data in domain III (DIII) of the envelope (E) gene in serial plasma samples ( <jats:italic>n</jats:italic> = 49) taken from 17 patients infected with DENV type 1 (DENV-1), totaling some 8,458 clones. Statistically rigorous approaches were employed to account for artifactual variants resulting from amplification and sequencing, which we suggest have played a major role in previous studies of intrahost genetic variation. Accordingly, nucleotide sequence diversities of viral populations were very low, with conservative estimates of the average levels of genetic diversity ranging from 0 to 0.0013. Despite such sequence conservation, we observed clear evidence for mixed infection, with the presence of multiple phylogenetically distinct lineages present within the same host, while the presence of stop codon mutations in some samples suggests the action of complementation. In contrast to some previous studies we observed no relationship between the extent and pattern of DENV-1 genetic diversity and disease severity, immune status, or level of viremia. </jats:p> |
| doi_str_mv | 10.1128/jvi.05985-11 |
| facet_avail | Online, Free |
| finc_class_facet | Medizin, Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qdmkuMDU5ODUtMTE |
| imprint | American Society for Microbiology, 2012 |
| imprint_str_mv | American Society for Microbiology, 2012 |
| institution | DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15 |
| issn | 0022-538X, 1098-5514 |
| issn_str_mv | 0022-538X, 1098-5514 |
| language | English |
| last_indexed | 2024-03-01T14:53:55.433Z |
| match_str | thai2012highresolutionanalysisofintrahostgeneticdiversityindenguevirusserotype1infectionidentifiesmixedinfections |
| mega_collection | American Society for Microbiology (CrossRef) |
| physical | 835-843 |
| publishDate | 2012 |
| publishDateSort | 2012 |
| publisher | American Society for Microbiology |
| record_format | ai |
| recordtype | ai |
| series | Journal of Virology |
| source_id | 49 |
| spelling | Thai, Khoa T. D. Henn, Matthew R. Zody, Michael C. Tricou, Vianney Nguyet, Nguyen Minh Charlebois, Patrick Lennon, Niall J. Green, Lisa de Vries, Peter J. Hien, Tran Tinh Farrar, Jeremy van Doorn, H. Rogier de Jong, Menno D. Birren, Bruce W. Holmes, Edward C. Simmons, Cameron P. 0022-538X 1098-5514 American Society for Microbiology Virology Insect Science Immunology Microbiology http://dx.doi.org/10.1128/jvi.05985-11 <jats:title>ABSTRACT</jats:title> <jats:p> Little is known about the rate at which genetic variation is generated within intrahost populations of dengue virus (DENV) and what implications this diversity has for dengue pathogenesis, disease severity, and host immunity. Previous studies of intrahost DENV variation have used a low frequency of sampling and/or experimental methods that do not fully account for errors generated through amplification and sequencing of viral RNAs. We investigated the extent and pattern of genetic diversity in sequence data in domain III (DIII) of the envelope (E) gene in serial plasma samples ( <jats:italic>n</jats:italic> = 49) taken from 17 patients infected with DENV type 1 (DENV-1), totaling some 8,458 clones. Statistically rigorous approaches were employed to account for artifactual variants resulting from amplification and sequencing, which we suggest have played a major role in previous studies of intrahost genetic variation. Accordingly, nucleotide sequence diversities of viral populations were very low, with conservative estimates of the average levels of genetic diversity ranging from 0 to 0.0013. Despite such sequence conservation, we observed clear evidence for mixed infection, with the presence of multiple phylogenetically distinct lineages present within the same host, while the presence of stop codon mutations in some samples suggests the action of complementation. In contrast to some previous studies we observed no relationship between the extent and pattern of DENV-1 genetic diversity and disease severity, immune status, or level of viremia. </jats:p> High-Resolution Analysis of Intrahost Genetic Diversity in Dengue Virus Serotype 1 Infection Identifies Mixed Infections Journal of Virology |
| spellingShingle | Thai, Khoa T. D., Henn, Matthew R., Zody, Michael C., Tricou, Vianney, Nguyet, Nguyen Minh, Charlebois, Patrick, Lennon, Niall J., Green, Lisa, de Vries, Peter J., Hien, Tran Tinh, Farrar, Jeremy, van Doorn, H. Rogier, de Jong, Menno D., Birren, Bruce W., Holmes, Edward C., Simmons, Cameron P., Journal of Virology, High-Resolution Analysis of Intrahost Genetic Diversity in Dengue Virus Serotype 1 Infection Identifies Mixed Infections, Virology, Insect Science, Immunology, Microbiology |
| title | High-Resolution Analysis of Intrahost Genetic Diversity in Dengue Virus Serotype 1 Infection Identifies Mixed Infections |
| title_full | High-Resolution Analysis of Intrahost Genetic Diversity in Dengue Virus Serotype 1 Infection Identifies Mixed Infections |
| title_fullStr | High-Resolution Analysis of Intrahost Genetic Diversity in Dengue Virus Serotype 1 Infection Identifies Mixed Infections |
| title_full_unstemmed | High-Resolution Analysis of Intrahost Genetic Diversity in Dengue Virus Serotype 1 Infection Identifies Mixed Infections |
| title_short | High-Resolution Analysis of Intrahost Genetic Diversity in Dengue Virus Serotype 1 Infection Identifies Mixed Infections |
| title_sort | high-resolution analysis of intrahost genetic diversity in dengue virus serotype 1 infection identifies mixed infections |
| title_unstemmed | High-Resolution Analysis of Intrahost Genetic Diversity in Dengue Virus Serotype 1 Infection Identifies Mixed Infections |
| topic | Virology, Insect Science, Immunology, Microbiology |
| url | http://dx.doi.org/10.1128/jvi.05985-11 |