Comprehensive Analysis of Varicella-Zoster Virus Proteins Using a New Monoclonal Antibody Collection

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Bibliographische Detailangaben
Zeitschriftentitel:	Journal of Virology
Personen und Körperschaften:	Lenac Roviš, Tihana, Bailer, Susanne M., Pothineni, Venkata R., Ouwendijk, Werner J. D., Šimić, Hrvoje, Babić, Marina, Miklić, Karmela, Malić, Suzana, Verweij, Marieke C., Baiker, Armin, Gonzalez, Orland, von Brunn, Albrecht, Zimmer, Ralf, Früh, Klaus, Verjans, Georges M. G. M., Jonjić, Stipan, Haas, Jürgen
In:	Journal of Virology, 87, 2013, 12, S. 6943-6954
Format:	E-Article
Sprache:	Englisch
veröffentlicht:	American Society for Microbiology
Schlagwörter:	Virology Insect Science Immunology Microbiology

Details
Zusammenfassung:	<jats:title>ABSTRACT</jats:title> <jats:p> Varicella-zoster virus (VZV) is the etiological agent of chickenpox and shingles. Due to the virus's restricted host and cell type tropism and the lack of tools for VZV proteomics, it is one of the least-characterized human herpesviruses. We generated 251 monoclonal antibodies (MAbs) against 59 of the 71 (83%) currently known unique VZV proteins to characterize VZV protein expression <jats:italic>in vitro</jats:italic> and <jats:italic>in situ</jats:italic> . Using this new set of MAbs, 44 viral proteins were detected by Western blotting (WB) and indirect immunofluorescence (IF); 13 were detected by WB only, and 2 were detected by IF only. A large proportion of viral proteins was analyzed for the first time in the context of virus infection. Our study revealed the subcellular localization of 46 proteins, 14 of which were analyzed in detail by confocal microscopy. Seven viral proteins were analyzed in time course experiments and showed a cascade-like temporal gene expression pattern similar to those of other herpesviruses. Furthermore, selected MAbs tested positive on human skin lesions by using immunohistochemistry, demonstrating the wide applicability of the MAb collection. Finally, a significant portion of the VZV-specific antibodies reacted with orthologs of simian varicella virus (SVV), thus enabling the systematic analysis of varicella in a nonhuman primate model system. In summary, this study provides insight into the potential function of numerous VZV proteins and novel tools to systematically study VZV and SVV pathogenesis. </jats:p>
Umfang:	6943-6954
ISSN:	0022-538X 1098-5514
DOI:	10.1128/jvi.00407-13