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Control of the Ferric Citrate Transport System of Escherichia coli : Mutations in Region 2.1 of the FecI Extracytoplasmic-Function Sigma Factor Suppress Mutat...
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| Zeitschriftentitel: | Journal of Bacteriology |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Journal of Bacteriology, 183, 2001, 1, S. 162-170 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Stiefel, Alfred Mahren, Susanne Ochs, Martina Schindler, Petra T. Enz, Sabine Braun, Volkmar Stiefel, Alfred Mahren, Susanne Ochs, Martina Schindler, Petra T. Enz, Sabine Braun, Volkmar |
|---|---|
| author |
Stiefel, Alfred Mahren, Susanne Ochs, Martina Schindler, Petra T. Enz, Sabine Braun, Volkmar |
| spellingShingle |
Stiefel, Alfred Mahren, Susanne Ochs, Martina Schindler, Petra T. Enz, Sabine Braun, Volkmar Journal of Bacteriology Control of the Ferric Citrate Transport System of Escherichia coli : Mutations in Region 2.1 of the FecI Extracytoplasmic-Function Sigma Factor Suppress Mutations in the FecR Transmembrane Regulatory Protein Molecular Biology Microbiology |
| author_sort |
stiefel, alfred |
| spelling |
Stiefel, Alfred Mahren, Susanne Ochs, Martina Schindler, Petra T. Enz, Sabine Braun, Volkmar 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.183.1.162-170.2001 <jats:title>ABSTRACT</jats:title> <jats:p> Transcription of the ferric citrate transport genes is initiated by binding of ferric citrate to the FecA protein in the outer membrane of <jats:italic>Escherichia coli</jats:italic> K-12. Bound ferric citrate does not have to be transported but initiates a signal that is transmitted by FecA across the outer membrane and by FecR across the cytoplasmic membrane into the cytoplasm, where the FecI extracytoplasmic-function (ECF) sigma factor becomes active. In this study, we isolated transcription initiation-negative missense mutants in the cytoplasmic region of FecR that were located at four sites, L13Q, W19R, W39R, and W50R, which are highly conserved in FecR-like open reading frames of the <jats:italic>Pseudomonas aeruginosa</jats:italic> , <jats:italic>Pseudomonas putida</jats:italic> , <jats:italic>Bordetella pertussis</jats:italic> , <jats:italic>Bordetella bronchiseptica</jats:italic> , and <jats:italic>Caulobacter crescentus</jats:italic> genomes. The cytoplasmic portion of the FecR mutant proteins, FecR <jats:sub>1–85</jats:sub> , did not interact with wild-type FecI, in contrast to wild-type FecR <jats:sub>1–85</jats:sub> , which induced FecI-mediated <jats:italic>fecB</jats:italic> transport gene transcription. Two missense mutations in region 2.1 of FecI, S15A and H20E, partially restored induction of ferric citrate transport gene induction of the <jats:italic>fecR</jats:italic> mutants by ferric citrate. Region 2.1 of ς <jats:sup>70</jats:sup> is thought to bind RNA polymerase core enzyme; the residual activity of mutated FecI in the absence of FecR, however, was not higher than that of wild-type FecI. In addition, missense mutations in the <jats:italic>fecI</jats:italic> promoter region resulted in a twofold increased transcription in <jats:italic>fecR</jats:italic> wild-type cells and a partial restoration of <jats:italic>fec</jats:italic> transport gene transcription in the <jats:italic>fecR</jats:italic> mutants. The mutations reduced binding of the Fe <jats:sup>2+</jats:sup> Fur repressor and as a consequence enhanced <jats:italic>fecI</jats:italic> transcription. The data reveal properties of the FecI ECF factor distinct from those of ς <jats:sup>70</jats:sup> and further support the novel transcription initiation model in which the cytoplasmic portion of FecR is important for FecI activity. </jats:p> Control of the Ferric Citrate Transport System of <i>Escherichia coli</i> : Mutations in Region 2.1 of the FecI Extracytoplasmic-Function Sigma Factor Suppress Mutations in the FecR Transmembrane Regulatory Protein Journal of Bacteriology |
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American Society for Microbiology, 2001 |
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American Society for Microbiology, 2001 |
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0021-9193 1098-5530 |
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American Society for Microbiology |
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Journal of Bacteriology |
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| title |
Control of the Ferric Citrate Transport System of Escherichia coli : Mutations in Region 2.1 of the FecI Extracytoplasmic-Function Sigma Factor Suppress Mutations in the FecR Transmembrane Regulatory Protein |
| title_unstemmed |
Control of the Ferric Citrate Transport System of Escherichia coli : Mutations in Region 2.1 of the FecI Extracytoplasmic-Function Sigma Factor Suppress Mutations in the FecR Transmembrane Regulatory Protein |
| title_full |
Control of the Ferric Citrate Transport System of Escherichia coli : Mutations in Region 2.1 of the FecI Extracytoplasmic-Function Sigma Factor Suppress Mutations in the FecR Transmembrane Regulatory Protein |
| title_fullStr |
Control of the Ferric Citrate Transport System of Escherichia coli : Mutations in Region 2.1 of the FecI Extracytoplasmic-Function Sigma Factor Suppress Mutations in the FecR Transmembrane Regulatory Protein |
| title_full_unstemmed |
Control of the Ferric Citrate Transport System of Escherichia coli : Mutations in Region 2.1 of the FecI Extracytoplasmic-Function Sigma Factor Suppress Mutations in the FecR Transmembrane Regulatory Protein |
| title_short |
Control of the Ferric Citrate Transport System of Escherichia coli : Mutations in Region 2.1 of the FecI Extracytoplasmic-Function Sigma Factor Suppress Mutations in the FecR Transmembrane Regulatory Protein |
| title_sort |
control of the ferric citrate transport system of
<i>escherichia coli</i>
: mutations in region 2.1 of the feci extracytoplasmic-function sigma factor suppress mutations in the fecr transmembrane regulatory protein |
| topic |
Molecular Biology Microbiology |
| url |
http://dx.doi.org/10.1128/jb.183.1.162-170.2001 |
| publishDate |
2001 |
| physical |
162-170 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Transcription of the ferric citrate transport genes is initiated by binding of ferric citrate to the FecA protein in the outer membrane of
<jats:italic>Escherichia coli</jats:italic>
K-12. Bound ferric citrate does not have to be transported but initiates a signal that is transmitted by FecA across the outer membrane and by FecR across the cytoplasmic membrane into the cytoplasm, where the FecI extracytoplasmic-function (ECF) sigma factor becomes active. In this study, we isolated transcription initiation-negative missense mutants in the cytoplasmic region of FecR that were located at four sites, L13Q, W19R, W39R, and W50R, which are highly conserved in FecR-like open reading frames of the
<jats:italic>Pseudomonas aeruginosa</jats:italic>
,
<jats:italic>Pseudomonas putida</jats:italic>
,
<jats:italic>Bordetella pertussis</jats:italic>
,
<jats:italic>Bordetella bronchiseptica</jats:italic>
, and
<jats:italic>Caulobacter crescentus</jats:italic>
genomes. The cytoplasmic portion of the FecR mutant proteins, FecR
<jats:sub>1–85</jats:sub>
, did not interact with wild-type FecI, in contrast to wild-type FecR
<jats:sub>1–85</jats:sub>
, which induced FecI-mediated
<jats:italic>fecB</jats:italic>
transport gene transcription. Two missense mutations in region 2.1 of FecI, S15A and H20E, partially restored induction of ferric citrate transport gene induction of the
<jats:italic>fecR</jats:italic>
mutants by ferric citrate. Region 2.1 of ς
<jats:sup>70</jats:sup>
is thought to bind RNA polymerase core enzyme; the residual activity of mutated FecI in the absence of FecR, however, was not higher than that of wild-type FecI. In addition, missense mutations in the
<jats:italic>fecI</jats:italic>
promoter region resulted in a twofold increased transcription in
<jats:italic>fecR</jats:italic>
wild-type cells and a partial restoration of
<jats:italic>fec</jats:italic>
transport gene transcription in the
<jats:italic>fecR</jats:italic>
mutants. The mutations reduced binding of the Fe
<jats:sup>2+</jats:sup>
Fur repressor and as a consequence enhanced
<jats:italic>fecI</jats:italic>
transcription. The data reveal properties of the FecI ECF factor distinct from those of ς
<jats:sup>70</jats:sup>
and further support the novel transcription initiation model in which the cytoplasmic portion of FecR is important for FecI activity.
</jats:p> |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> Transcription of the ferric citrate transport genes is initiated by binding of ferric citrate to the FecA protein in the outer membrane of <jats:italic>Escherichia coli</jats:italic> K-12. Bound ferric citrate does not have to be transported but initiates a signal that is transmitted by FecA across the outer membrane and by FecR across the cytoplasmic membrane into the cytoplasm, where the FecI extracytoplasmic-function (ECF) sigma factor becomes active. In this study, we isolated transcription initiation-negative missense mutants in the cytoplasmic region of FecR that were located at four sites, L13Q, W19R, W39R, and W50R, which are highly conserved in FecR-like open reading frames of the <jats:italic>Pseudomonas aeruginosa</jats:italic> , <jats:italic>Pseudomonas putida</jats:italic> , <jats:italic>Bordetella pertussis</jats:italic> , <jats:italic>Bordetella bronchiseptica</jats:italic> , and <jats:italic>Caulobacter crescentus</jats:italic> genomes. The cytoplasmic portion of the FecR mutant proteins, FecR <jats:sub>1–85</jats:sub> , did not interact with wild-type FecI, in contrast to wild-type FecR <jats:sub>1–85</jats:sub> , which induced FecI-mediated <jats:italic>fecB</jats:italic> transport gene transcription. Two missense mutations in region 2.1 of FecI, S15A and H20E, partially restored induction of ferric citrate transport gene induction of the <jats:italic>fecR</jats:italic> mutants by ferric citrate. Region 2.1 of ς <jats:sup>70</jats:sup> is thought to bind RNA polymerase core enzyme; the residual activity of mutated FecI in the absence of FecR, however, was not higher than that of wild-type FecI. In addition, missense mutations in the <jats:italic>fecI</jats:italic> promoter region resulted in a twofold increased transcription in <jats:italic>fecR</jats:italic> wild-type cells and a partial restoration of <jats:italic>fec</jats:italic> transport gene transcription in the <jats:italic>fecR</jats:italic> mutants. The mutations reduced binding of the Fe <jats:sup>2+</jats:sup> Fur repressor and as a consequence enhanced <jats:italic>fecI</jats:italic> transcription. The data reveal properties of the FecI ECF factor distinct from those of ς <jats:sup>70</jats:sup> and further support the novel transcription initiation model in which the cytoplasmic portion of FecR is important for FecI activity. </jats:p> |
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| spelling | Stiefel, Alfred Mahren, Susanne Ochs, Martina Schindler, Petra T. Enz, Sabine Braun, Volkmar 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.183.1.162-170.2001 <jats:title>ABSTRACT</jats:title> <jats:p> Transcription of the ferric citrate transport genes is initiated by binding of ferric citrate to the FecA protein in the outer membrane of <jats:italic>Escherichia coli</jats:italic> K-12. Bound ferric citrate does not have to be transported but initiates a signal that is transmitted by FecA across the outer membrane and by FecR across the cytoplasmic membrane into the cytoplasm, where the FecI extracytoplasmic-function (ECF) sigma factor becomes active. In this study, we isolated transcription initiation-negative missense mutants in the cytoplasmic region of FecR that were located at four sites, L13Q, W19R, W39R, and W50R, which are highly conserved in FecR-like open reading frames of the <jats:italic>Pseudomonas aeruginosa</jats:italic> , <jats:italic>Pseudomonas putida</jats:italic> , <jats:italic>Bordetella pertussis</jats:italic> , <jats:italic>Bordetella bronchiseptica</jats:italic> , and <jats:italic>Caulobacter crescentus</jats:italic> genomes. The cytoplasmic portion of the FecR mutant proteins, FecR <jats:sub>1–85</jats:sub> , did not interact with wild-type FecI, in contrast to wild-type FecR <jats:sub>1–85</jats:sub> , which induced FecI-mediated <jats:italic>fecB</jats:italic> transport gene transcription. Two missense mutations in region 2.1 of FecI, S15A and H20E, partially restored induction of ferric citrate transport gene induction of the <jats:italic>fecR</jats:italic> mutants by ferric citrate. Region 2.1 of ς <jats:sup>70</jats:sup> is thought to bind RNA polymerase core enzyme; the residual activity of mutated FecI in the absence of FecR, however, was not higher than that of wild-type FecI. In addition, missense mutations in the <jats:italic>fecI</jats:italic> promoter region resulted in a twofold increased transcription in <jats:italic>fecR</jats:italic> wild-type cells and a partial restoration of <jats:italic>fec</jats:italic> transport gene transcription in the <jats:italic>fecR</jats:italic> mutants. The mutations reduced binding of the Fe <jats:sup>2+</jats:sup> Fur repressor and as a consequence enhanced <jats:italic>fecI</jats:italic> transcription. The data reveal properties of the FecI ECF factor distinct from those of ς <jats:sup>70</jats:sup> and further support the novel transcription initiation model in which the cytoplasmic portion of FecR is important for FecI activity. </jats:p> Control of the Ferric Citrate Transport System of <i>Escherichia coli</i> : Mutations in Region 2.1 of the FecI Extracytoplasmic-Function Sigma Factor Suppress Mutations in the FecR Transmembrane Regulatory Protein Journal of Bacteriology |
| spellingShingle | Stiefel, Alfred, Mahren, Susanne, Ochs, Martina, Schindler, Petra T., Enz, Sabine, Braun, Volkmar, Journal of Bacteriology, Control of the Ferric Citrate Transport System of Escherichia coli : Mutations in Region 2.1 of the FecI Extracytoplasmic-Function Sigma Factor Suppress Mutations in the FecR Transmembrane Regulatory Protein, Molecular Biology, Microbiology |
| title | Control of the Ferric Citrate Transport System of Escherichia coli : Mutations in Region 2.1 of the FecI Extracytoplasmic-Function Sigma Factor Suppress Mutations in the FecR Transmembrane Regulatory Protein |
| title_full | Control of the Ferric Citrate Transport System of Escherichia coli : Mutations in Region 2.1 of the FecI Extracytoplasmic-Function Sigma Factor Suppress Mutations in the FecR Transmembrane Regulatory Protein |
| title_fullStr | Control of the Ferric Citrate Transport System of Escherichia coli : Mutations in Region 2.1 of the FecI Extracytoplasmic-Function Sigma Factor Suppress Mutations in the FecR Transmembrane Regulatory Protein |
| title_full_unstemmed | Control of the Ferric Citrate Transport System of Escherichia coli : Mutations in Region 2.1 of the FecI Extracytoplasmic-Function Sigma Factor Suppress Mutations in the FecR Transmembrane Regulatory Protein |
| title_short | Control of the Ferric Citrate Transport System of Escherichia coli : Mutations in Region 2.1 of the FecI Extracytoplasmic-Function Sigma Factor Suppress Mutations in the FecR Transmembrane Regulatory Protein |
| title_sort | control of the ferric citrate transport system of <i>escherichia coli</i> : mutations in region 2.1 of the feci extracytoplasmic-function sigma factor suppress mutations in the fecr transmembrane regulatory protein |
| title_unstemmed | Control of the Ferric Citrate Transport System of Escherichia coli : Mutations in Region 2.1 of the FecI Extracytoplasmic-Function Sigma Factor Suppress Mutations in the FecR Transmembrane Regulatory Protein |
| topic | Molecular Biology, Microbiology |
| url | http://dx.doi.org/10.1128/jb.183.1.162-170.2001 |