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Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF
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| Zeitschriftentitel: | Journal of Bacteriology |
|---|---|
| Personen und Körperschaften: | |
| In: | Journal of Bacteriology, 156, 1983, 2, S. 800-808 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Jentsch, S Jentsch, S |
|---|---|
| author |
Jentsch, S |
| spellingShingle |
Jentsch, S Journal of Bacteriology Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF Molecular Biology Microbiology |
| author_sort |
jentsch, s |
| spelling |
Jentsch, S 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.156.2.800-808.1983 <jats:p>The sequence specificities of three Bacillus subtilis restriction/modification systems were established: (i) BsuM (CTCGAG), an isoschizomer to XhoI; (ii) BsuE (CGCG), an isoschizomer to FnuDII; and (iii) BsuF (CCGG), an isoschizomer to MspI, HpaII. The BsuM modification enzyme methylates the 3' cytosine of the recognition sequence. The BsuF modification enzyme methylates the 5' cytosine of the sequence, rendering such sites resistant to MspI degradation and leaving the majority of sites sensitive to HpaII degradation.</jats:p> Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF Journal of Bacteriology |
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10.1128/jb.156.2.800-808.1983 |
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American Society for Microbiology, 1983 |
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American Society for Microbiology, 1983 |
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0021-9193 1098-5530 |
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0021-9193 1098-5530 |
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1983 |
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American Society for Microbiology |
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Journal of Bacteriology |
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49 |
| title |
Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF |
| title_unstemmed |
Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF |
| title_full |
Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF |
| title_fullStr |
Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF |
| title_full_unstemmed |
Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF |
| title_short |
Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF |
| title_sort |
restriction and modification in bacillus subtilis: sequence specificities of restriction/modification systems bsum, bsue, and bsuf |
| topic |
Molecular Biology Microbiology |
| url |
http://dx.doi.org/10.1128/jb.156.2.800-808.1983 |
| publishDate |
1983 |
| physical |
800-808 |
| description |
<jats:p>The sequence specificities of three Bacillus subtilis restriction/modification systems were established: (i) BsuM (CTCGAG), an isoschizomer to XhoI; (ii) BsuE (CGCG), an isoschizomer to FnuDII; and (iii) BsuF (CCGG), an isoschizomer to MspI, HpaII. The BsuM modification enzyme methylates the 3' cytosine of the recognition sequence. The BsuF modification enzyme methylates the 5' cytosine of the sequence, rendering such sites resistant to MspI degradation and leaving the majority of sites sensitive to HpaII degradation.</jats:p> |
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| author | Jentsch, S |
| author_facet | Jentsch, S, Jentsch, S |
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| description | <jats:p>The sequence specificities of three Bacillus subtilis restriction/modification systems were established: (i) BsuM (CTCGAG), an isoschizomer to XhoI; (ii) BsuE (CGCG), an isoschizomer to FnuDII; and (iii) BsuF (CCGG), an isoschizomer to MspI, HpaII. The BsuM modification enzyme methylates the 3' cytosine of the recognition sequence. The BsuF modification enzyme methylates the 5' cytosine of the sequence, rendering such sites resistant to MspI degradation and leaving the majority of sites sensitive to HpaII degradation.</jats:p> |
| doi_str_mv | 10.1128/jb.156.2.800-808.1983 |
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| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qYi4xNTYuMi44MDAtODA4LjE5ODM |
| imprint | American Society for Microbiology, 1983 |
| imprint_str_mv | American Society for Microbiology, 1983 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
| issn | 0021-9193, 1098-5530 |
| issn_str_mv | 0021-9193, 1098-5530 |
| language | English |
| last_indexed | 2024-03-01T15:46:28.088Z |
| match_str | jentsch1983restrictionandmodificationinbacillussubtilissequencespecificitiesofrestrictionmodificationsystemsbsumbsueandbsuf |
| mega_collection | American Society for Microbiology (CrossRef) |
| physical | 800-808 |
| publishDate | 1983 |
| publishDateSort | 1983 |
| publisher | American Society for Microbiology |
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| recordtype | ai |
| series | Journal of Bacteriology |
| source_id | 49 |
| spelling | Jentsch, S 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.156.2.800-808.1983 <jats:p>The sequence specificities of three Bacillus subtilis restriction/modification systems were established: (i) BsuM (CTCGAG), an isoschizomer to XhoI; (ii) BsuE (CGCG), an isoschizomer to FnuDII; and (iii) BsuF (CCGG), an isoschizomer to MspI, HpaII. The BsuM modification enzyme methylates the 3' cytosine of the recognition sequence. The BsuF modification enzyme methylates the 5' cytosine of the sequence, rendering such sites resistant to MspI degradation and leaving the majority of sites sensitive to HpaII degradation.</jats:p> Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF Journal of Bacteriology |
| spellingShingle | Jentsch, S, Journal of Bacteriology, Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF, Molecular Biology, Microbiology |
| title | Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF |
| title_full | Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF |
| title_fullStr | Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF |
| title_full_unstemmed | Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF |
| title_short | Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF |
| title_sort | restriction and modification in bacillus subtilis: sequence specificities of restriction/modification systems bsum, bsue, and bsuf |
| title_unstemmed | Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF |
| topic | Molecular Biology, Microbiology |
| url | http://dx.doi.org/10.1128/jb.156.2.800-808.1983 |