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Roles of ExoI and SbcCD Nucleases in “Reckless” DNA Degradation in recA Mutants of Escherichia coli
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| Zeitschriftentitel: | Journal of Bacteriology |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Journal of Bacteriology, 191, 2009, 5, S. 1677-1687 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Zahradka, Ksenija Buljubašić, Maja Petranović, Mirjana Zahradka, Davor Zahradka, Ksenija Buljubašić, Maja Petranović, Mirjana Zahradka, Davor |
|---|---|
| author |
Zahradka, Ksenija Buljubašić, Maja Petranović, Mirjana Zahradka, Davor |
| spellingShingle |
Zahradka, Ksenija Buljubašić, Maja Petranović, Mirjana Zahradka, Davor Journal of Bacteriology Roles of ExoI and SbcCD Nucleases in “Reckless” DNA Degradation in recA Mutants of Escherichia coli Molecular Biology Microbiology |
| author_sort |
zahradka, ksenija |
| spelling |
Zahradka, Ksenija Buljubašić, Maja Petranović, Mirjana Zahradka, Davor 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.01877-07 <jats:title>ABSTRACT</jats:title> <jats:p> Exponentially growing <jats:italic>recA</jats:italic> mutant cells of <jats:italic>Escherichia coli</jats:italic> display pronounced DNA degradation that starts at the sites of DNA damage and depends on RecBCD nuclease (ExoV) activity. As a consequence of this “reckless” DNA degradation, populations of <jats:italic>recA</jats:italic> mutants contain a large proportion of anucleate cells. We have found that both DNA degradation and anucleate-cell production are efficiently suppressed by mutations in the <jats:italic>xonA</jats:italic> ( <jats:italic>sbcB</jats:italic> ) and <jats:italic>sbcD</jats:italic> genes. The suppressive effects of these mutations were observed in normally grown, as well as in UV-irradiated, <jats:italic>recA</jats:italic> cells. The products of the <jats:italic>xonA</jats:italic> and <jats:italic>sbcD</jats:italic> genes are known to code for the ExoI and SbcCD nucleases, respectively. Since both <jats:italic>xonA</jats:italic> and <jats:italic>sbcD</jats:italic> mutations are required for strong suppression of DNA degradation while individual mutations have only a weak suppressive effect, we infer that ExoI and SbcCD play partially redundant roles in regulating DNA degradation in <jats:italic>recA</jats:italic> cells. We suggest that their roles might be in processing (blunting) DNA ends, thereby producing suitable substrates for RecBCD binding. </jats:p> Roles of ExoI and SbcCD Nucleases in “Reckless” DNA Degradation in <i>recA</i> Mutants of <i>Escherichia coli</i> Journal of Bacteriology |
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| title |
Roles of ExoI and SbcCD Nucleases in “Reckless” DNA Degradation in recA Mutants of Escherichia coli |
| title_unstemmed |
Roles of ExoI and SbcCD Nucleases in “Reckless” DNA Degradation in recA Mutants of Escherichia coli |
| title_full |
Roles of ExoI and SbcCD Nucleases in “Reckless” DNA Degradation in recA Mutants of Escherichia coli |
| title_fullStr |
Roles of ExoI and SbcCD Nucleases in “Reckless” DNA Degradation in recA Mutants of Escherichia coli |
| title_full_unstemmed |
Roles of ExoI and SbcCD Nucleases in “Reckless” DNA Degradation in recA Mutants of Escherichia coli |
| title_short |
Roles of ExoI and SbcCD Nucleases in “Reckless” DNA Degradation in recA Mutants of Escherichia coli |
| title_sort |
roles of exoi and sbccd nucleases in “reckless” dna degradation in
<i>reca</i>
mutants of
<i>escherichia coli</i> |
| topic |
Molecular Biology Microbiology |
| url |
http://dx.doi.org/10.1128/jb.01877-07 |
| publishDate |
2009 |
| physical |
1677-1687 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Exponentially growing
<jats:italic>recA</jats:italic>
mutant cells of
<jats:italic>Escherichia coli</jats:italic>
display pronounced DNA degradation that starts at the sites of DNA damage and depends on RecBCD nuclease (ExoV) activity. As a consequence of this “reckless” DNA degradation, populations of
<jats:italic>recA</jats:italic>
mutants contain a large proportion of anucleate cells. We have found that both DNA degradation and anucleate-cell production are efficiently suppressed by mutations in the
<jats:italic>xonA</jats:italic>
(
<jats:italic>sbcB</jats:italic>
) and
<jats:italic>sbcD</jats:italic>
genes. The suppressive effects of these mutations were observed in normally grown, as well as in UV-irradiated,
<jats:italic>recA</jats:italic>
cells. The products of the
<jats:italic>xonA</jats:italic>
and
<jats:italic>sbcD</jats:italic>
genes are known to code for the ExoI and SbcCD nucleases, respectively. Since both
<jats:italic>xonA</jats:italic>
and
<jats:italic>sbcD</jats:italic>
mutations are required for strong suppression of DNA degradation while individual mutations have only a weak suppressive effect, we infer that ExoI and SbcCD play partially redundant roles in regulating DNA degradation in
<jats:italic>recA</jats:italic>
cells. We suggest that their roles might be in processing (blunting) DNA ends, thereby producing suitable substrates for RecBCD binding.
</jats:p> |
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| author | Zahradka, Ksenija, Buljubašić, Maja, Petranović, Mirjana, Zahradka, Davor |
| author_facet | Zahradka, Ksenija, Buljubašić, Maja, Petranović, Mirjana, Zahradka, Davor, Zahradka, Ksenija, Buljubašić, Maja, Petranović, Mirjana, Zahradka, Davor |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> Exponentially growing <jats:italic>recA</jats:italic> mutant cells of <jats:italic>Escherichia coli</jats:italic> display pronounced DNA degradation that starts at the sites of DNA damage and depends on RecBCD nuclease (ExoV) activity. As a consequence of this “reckless” DNA degradation, populations of <jats:italic>recA</jats:italic> mutants contain a large proportion of anucleate cells. We have found that both DNA degradation and anucleate-cell production are efficiently suppressed by mutations in the <jats:italic>xonA</jats:italic> ( <jats:italic>sbcB</jats:italic> ) and <jats:italic>sbcD</jats:italic> genes. The suppressive effects of these mutations were observed in normally grown, as well as in UV-irradiated, <jats:italic>recA</jats:italic> cells. The products of the <jats:italic>xonA</jats:italic> and <jats:italic>sbcD</jats:italic> genes are known to code for the ExoI and SbcCD nucleases, respectively. Since both <jats:italic>xonA</jats:italic> and <jats:italic>sbcD</jats:italic> mutations are required for strong suppression of DNA degradation while individual mutations have only a weak suppressive effect, we infer that ExoI and SbcCD play partially redundant roles in regulating DNA degradation in <jats:italic>recA</jats:italic> cells. We suggest that their roles might be in processing (blunting) DNA ends, thereby producing suitable substrates for RecBCD binding. </jats:p> |
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| institution | DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3 |
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| physical | 1677-1687 |
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| spelling | Zahradka, Ksenija Buljubašić, Maja Petranović, Mirjana Zahradka, Davor 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.01877-07 <jats:title>ABSTRACT</jats:title> <jats:p> Exponentially growing <jats:italic>recA</jats:italic> mutant cells of <jats:italic>Escherichia coli</jats:italic> display pronounced DNA degradation that starts at the sites of DNA damage and depends on RecBCD nuclease (ExoV) activity. As a consequence of this “reckless” DNA degradation, populations of <jats:italic>recA</jats:italic> mutants contain a large proportion of anucleate cells. We have found that both DNA degradation and anucleate-cell production are efficiently suppressed by mutations in the <jats:italic>xonA</jats:italic> ( <jats:italic>sbcB</jats:italic> ) and <jats:italic>sbcD</jats:italic> genes. The suppressive effects of these mutations were observed in normally grown, as well as in UV-irradiated, <jats:italic>recA</jats:italic> cells. The products of the <jats:italic>xonA</jats:italic> and <jats:italic>sbcD</jats:italic> genes are known to code for the ExoI and SbcCD nucleases, respectively. Since both <jats:italic>xonA</jats:italic> and <jats:italic>sbcD</jats:italic> mutations are required for strong suppression of DNA degradation while individual mutations have only a weak suppressive effect, we infer that ExoI and SbcCD play partially redundant roles in regulating DNA degradation in <jats:italic>recA</jats:italic> cells. We suggest that their roles might be in processing (blunting) DNA ends, thereby producing suitable substrates for RecBCD binding. </jats:p> Roles of ExoI and SbcCD Nucleases in “Reckless” DNA Degradation in <i>recA</i> Mutants of <i>Escherichia coli</i> Journal of Bacteriology |
| spellingShingle | Zahradka, Ksenija, Buljubašić, Maja, Petranović, Mirjana, Zahradka, Davor, Journal of Bacteriology, Roles of ExoI and SbcCD Nucleases in “Reckless” DNA Degradation in recA Mutants of Escherichia coli, Molecular Biology, Microbiology |
| title | Roles of ExoI and SbcCD Nucleases in “Reckless” DNA Degradation in recA Mutants of Escherichia coli |
| title_full | Roles of ExoI and SbcCD Nucleases in “Reckless” DNA Degradation in recA Mutants of Escherichia coli |
| title_fullStr | Roles of ExoI and SbcCD Nucleases in “Reckless” DNA Degradation in recA Mutants of Escherichia coli |
| title_full_unstemmed | Roles of ExoI and SbcCD Nucleases in “Reckless” DNA Degradation in recA Mutants of Escherichia coli |
| title_short | Roles of ExoI and SbcCD Nucleases in “Reckless” DNA Degradation in recA Mutants of Escherichia coli |
| title_sort | roles of exoi and sbccd nucleases in “reckless” dna degradation in <i>reca</i> mutants of <i>escherichia coli</i> |
| title_unstemmed | Roles of ExoI and SbcCD Nucleases in “Reckless” DNA Degradation in recA Mutants of Escherichia coli |
| topic | Molecular Biology, Microbiology |
| url | http://dx.doi.org/10.1128/jb.01877-07 |