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The Global Transcriptional Responses of Bacillus anthracis Sterne (34F 2 ) and a Δ sodA1 Mutant to Paraquat Reveal...
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| Zeitschriftentitel: | Journal of Bacteriology |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Journal of Bacteriology, 189, 2007, 11, S. 3996-4013 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Passalacqua, Karla D. Bergman, Nicholas H. Lee, Jung Yeop Sherman, David H. Hanna, Philip C. Passalacqua, Karla D. Bergman, Nicholas H. Lee, Jung Yeop Sherman, David H. Hanna, Philip C. |
|---|---|
| author |
Passalacqua, Karla D. Bergman, Nicholas H. Lee, Jung Yeop Sherman, David H. Hanna, Philip C. |
| spellingShingle |
Passalacqua, Karla D. Bergman, Nicholas H. Lee, Jung Yeop Sherman, David H. Hanna, Philip C. Journal of Bacteriology The Global Transcriptional Responses of Bacillus anthracis Sterne (34F 2 ) and a Δ sodA1 Mutant to Paraquat Reveal Metal Ion Homeostasis Imbalances during Endogenous Superoxide Stress Molecular Biology Microbiology |
| author_sort |
passalacqua, karla d. |
| spelling |
Passalacqua, Karla D. Bergman, Nicholas H. Lee, Jung Yeop Sherman, David H. Hanna, Philip C. 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.00185-07 <jats:title>ABSTRACT</jats:title> <jats:p> Microarray analyses were conducted to evaluate the paraquat-induced global transcriptional response of <jats:italic>Bacillus anthracis</jats:italic> Sterne (34F <jats:sub>2</jats:sub> ) to varying levels of endogenous superoxide stress. Data revealed that the transcription of genes putatively involved in metal/ion transport, bacillibactin siderophore biosynthesis, the glyoxalase pathway, and oxidoreductase activity was perturbed most significantly. A <jats:italic>B. anthracis</jats:italic> mutant lacking the superoxide dismutase gene <jats:italic>sodA1</jats:italic> (Δ <jats:italic>sodA1</jats:italic> ) had transcriptional responses to paraquat similar to, but notably larger than, those of the isogenic parental strain. A small, unique set of genes was found to be differentially expressed in the Δ <jats:italic>sodA1</jats:italic> mutant relative to the parental strain during growth in rich broth independently of induced oxidative stress. The bacillibactin siderophore biosynthetic genes were notably overexpressed in Sterne and Δ <jats:italic>sodA1</jats:italic> cells after treatment with paraquat. The bacillibactin siderophore itself was isolated from the supernatants and lysates of cells grown in iron-depleted medium and was detected at lower levels after treatment with paraquat. This suggests that, while transcriptional regulation of these genes is sensitive to changes in the redox environment, additional levels of posttranscriptional control may exist for bacillibactin biosynthesis, or the enzymatic siderophore pipeline may be compromised by intracellular superoxide stress or damage. The Δ <jats:italic>sodA1</jats:italic> mutant showed slower growth in a chelated iron-limiting medium but not in a metal-depleted medium, suggesting a connection between the intracellular redox state and iron/metal ion acquisition in <jats:italic>B. anthracis</jats:italic> . A double mutant lacking both the <jats:italic>sodA1</jats:italic> and <jats:italic>sodA2</jats:italic> genes (Δ <jats:italic>sodA1</jats:italic> Δ <jats:italic>sodA2</jats:italic> ) was attenuated for growth in manganese-depleted medium, suggesting a slight level of redundancy between <jats:italic>sodA1</jats:italic> and <jats:italic>sodA2</jats:italic> , and a role for the <jats:italic>sod</jats:italic> genes in manganese homeostasis. </jats:p> The Global Transcriptional Responses of <i>Bacillus anthracis</i> Sterne (34F <sub>2</sub> ) and a Δ <i>sodA1</i> Mutant to Paraquat Reveal Metal Ion Homeostasis Imbalances during Endogenous Superoxide Stress Journal of Bacteriology |
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10.1128/jb.00185-07 |
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Online Free |
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Biologie |
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ElectronicArticle |
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| imprint |
American Society for Microbiology, 2007 |
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American Society for Microbiology, 2007 |
| issn |
0021-9193 1098-5530 |
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0021-9193 1098-5530 |
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American Society for Microbiology (CrossRef) |
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2007 |
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American Society for Microbiology |
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ai |
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ai |
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Journal of Bacteriology |
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49 |
| title |
The Global Transcriptional Responses of Bacillus anthracis Sterne (34F 2 ) and a Δ sodA1 Mutant to Paraquat Reveal Metal Ion Homeostasis Imbalances during Endogenous Superoxide Stress |
| title_unstemmed |
The Global Transcriptional Responses of Bacillus anthracis Sterne (34F 2 ) and a Δ sodA1 Mutant to Paraquat Reveal Metal Ion Homeostasis Imbalances during Endogenous Superoxide Stress |
| title_full |
The Global Transcriptional Responses of Bacillus anthracis Sterne (34F 2 ) and a Δ sodA1 Mutant to Paraquat Reveal Metal Ion Homeostasis Imbalances during Endogenous Superoxide Stress |
| title_fullStr |
The Global Transcriptional Responses of Bacillus anthracis Sterne (34F 2 ) and a Δ sodA1 Mutant to Paraquat Reveal Metal Ion Homeostasis Imbalances during Endogenous Superoxide Stress |
| title_full_unstemmed |
The Global Transcriptional Responses of Bacillus anthracis Sterne (34F 2 ) and a Δ sodA1 Mutant to Paraquat Reveal Metal Ion Homeostasis Imbalances during Endogenous Superoxide Stress |
| title_short |
The Global Transcriptional Responses of Bacillus anthracis Sterne (34F 2 ) and a Δ sodA1 Mutant to Paraquat Reveal Metal Ion Homeostasis Imbalances during Endogenous Superoxide Stress |
| title_sort |
the global transcriptional responses of
<i>bacillus anthracis</i>
sterne (34f
<sub>2</sub>
) and a δ
<i>soda1</i>
mutant to paraquat reveal metal ion homeostasis imbalances during endogenous superoxide stress |
| topic |
Molecular Biology Microbiology |
| url |
http://dx.doi.org/10.1128/jb.00185-07 |
| publishDate |
2007 |
| physical |
3996-4013 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Microarray analyses were conducted to evaluate the paraquat-induced global transcriptional response of
<jats:italic>Bacillus anthracis</jats:italic>
Sterne (34F
<jats:sub>2</jats:sub>
) to varying levels of endogenous superoxide stress. Data revealed that the transcription of genes putatively involved in metal/ion transport, bacillibactin siderophore biosynthesis, the glyoxalase pathway, and oxidoreductase activity was perturbed most significantly. A
<jats:italic>B. anthracis</jats:italic>
mutant lacking the superoxide dismutase gene
<jats:italic>sodA1</jats:italic>
(Δ
<jats:italic>sodA1</jats:italic>
) had transcriptional responses to paraquat similar to, but notably larger than, those of the isogenic parental strain. A small, unique set of genes was found to be differentially expressed in the Δ
<jats:italic>sodA1</jats:italic>
mutant relative to the parental strain during growth in rich broth independently of induced oxidative stress. The bacillibactin siderophore biosynthetic genes were notably overexpressed in Sterne and Δ
<jats:italic>sodA1</jats:italic>
cells after treatment with paraquat. The bacillibactin siderophore itself was isolated from the supernatants and lysates of cells grown in iron-depleted medium and was detected at lower levels after treatment with paraquat. This suggests that, while transcriptional regulation of these genes is sensitive to changes in the redox environment, additional levels of posttranscriptional control may exist for bacillibactin biosynthesis, or the enzymatic siderophore pipeline may be compromised by intracellular superoxide stress or damage. The Δ
<jats:italic>sodA1</jats:italic>
mutant showed slower growth in a chelated iron-limiting medium but not in a metal-depleted medium, suggesting a connection between the intracellular redox state and iron/metal ion acquisition in
<jats:italic>B. anthracis</jats:italic>
. A double mutant lacking both the
<jats:italic>sodA1</jats:italic>
and
<jats:italic>sodA2</jats:italic>
genes (Δ
<jats:italic>sodA1</jats:italic>
Δ
<jats:italic>sodA2</jats:italic>
) was attenuated for growth in manganese-depleted medium, suggesting a slight level of redundancy between
<jats:italic>sodA1</jats:italic>
and
<jats:italic>sodA2</jats:italic>
, and a role for the
<jats:italic>sod</jats:italic>
genes in manganese homeostasis.
</jats:p> |
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| author | Passalacqua, Karla D., Bergman, Nicholas H., Lee, Jung Yeop, Sherman, David H., Hanna, Philip C. |
| author_facet | Passalacqua, Karla D., Bergman, Nicholas H., Lee, Jung Yeop, Sherman, David H., Hanna, Philip C., Passalacqua, Karla D., Bergman, Nicholas H., Lee, Jung Yeop, Sherman, David H., Hanna, Philip C. |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> Microarray analyses were conducted to evaluate the paraquat-induced global transcriptional response of <jats:italic>Bacillus anthracis</jats:italic> Sterne (34F <jats:sub>2</jats:sub> ) to varying levels of endogenous superoxide stress. Data revealed that the transcription of genes putatively involved in metal/ion transport, bacillibactin siderophore biosynthesis, the glyoxalase pathway, and oxidoreductase activity was perturbed most significantly. A <jats:italic>B. anthracis</jats:italic> mutant lacking the superoxide dismutase gene <jats:italic>sodA1</jats:italic> (Δ <jats:italic>sodA1</jats:italic> ) had transcriptional responses to paraquat similar to, but notably larger than, those of the isogenic parental strain. A small, unique set of genes was found to be differentially expressed in the Δ <jats:italic>sodA1</jats:italic> mutant relative to the parental strain during growth in rich broth independently of induced oxidative stress. The bacillibactin siderophore biosynthetic genes were notably overexpressed in Sterne and Δ <jats:italic>sodA1</jats:italic> cells after treatment with paraquat. The bacillibactin siderophore itself was isolated from the supernatants and lysates of cells grown in iron-depleted medium and was detected at lower levels after treatment with paraquat. This suggests that, while transcriptional regulation of these genes is sensitive to changes in the redox environment, additional levels of posttranscriptional control may exist for bacillibactin biosynthesis, or the enzymatic siderophore pipeline may be compromised by intracellular superoxide stress or damage. The Δ <jats:italic>sodA1</jats:italic> mutant showed slower growth in a chelated iron-limiting medium but not in a metal-depleted medium, suggesting a connection between the intracellular redox state and iron/metal ion acquisition in <jats:italic>B. anthracis</jats:italic> . A double mutant lacking both the <jats:italic>sodA1</jats:italic> and <jats:italic>sodA2</jats:italic> genes (Δ <jats:italic>sodA1</jats:italic> Δ <jats:italic>sodA2</jats:italic> ) was attenuated for growth in manganese-depleted medium, suggesting a slight level of redundancy between <jats:italic>sodA1</jats:italic> and <jats:italic>sodA2</jats:italic> , and a role for the <jats:italic>sod</jats:italic> genes in manganese homeostasis. </jats:p> |
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| spelling | Passalacqua, Karla D. Bergman, Nicholas H. Lee, Jung Yeop Sherman, David H. Hanna, Philip C. 0021-9193 1098-5530 American Society for Microbiology Molecular Biology Microbiology http://dx.doi.org/10.1128/jb.00185-07 <jats:title>ABSTRACT</jats:title> <jats:p> Microarray analyses were conducted to evaluate the paraquat-induced global transcriptional response of <jats:italic>Bacillus anthracis</jats:italic> Sterne (34F <jats:sub>2</jats:sub> ) to varying levels of endogenous superoxide stress. Data revealed that the transcription of genes putatively involved in metal/ion transport, bacillibactin siderophore biosynthesis, the glyoxalase pathway, and oxidoreductase activity was perturbed most significantly. A <jats:italic>B. anthracis</jats:italic> mutant lacking the superoxide dismutase gene <jats:italic>sodA1</jats:italic> (Δ <jats:italic>sodA1</jats:italic> ) had transcriptional responses to paraquat similar to, but notably larger than, those of the isogenic parental strain. A small, unique set of genes was found to be differentially expressed in the Δ <jats:italic>sodA1</jats:italic> mutant relative to the parental strain during growth in rich broth independently of induced oxidative stress. The bacillibactin siderophore biosynthetic genes were notably overexpressed in Sterne and Δ <jats:italic>sodA1</jats:italic> cells after treatment with paraquat. The bacillibactin siderophore itself was isolated from the supernatants and lysates of cells grown in iron-depleted medium and was detected at lower levels after treatment with paraquat. This suggests that, while transcriptional regulation of these genes is sensitive to changes in the redox environment, additional levels of posttranscriptional control may exist for bacillibactin biosynthesis, or the enzymatic siderophore pipeline may be compromised by intracellular superoxide stress or damage. The Δ <jats:italic>sodA1</jats:italic> mutant showed slower growth in a chelated iron-limiting medium but not in a metal-depleted medium, suggesting a connection between the intracellular redox state and iron/metal ion acquisition in <jats:italic>B. anthracis</jats:italic> . A double mutant lacking both the <jats:italic>sodA1</jats:italic> and <jats:italic>sodA2</jats:italic> genes (Δ <jats:italic>sodA1</jats:italic> Δ <jats:italic>sodA2</jats:italic> ) was attenuated for growth in manganese-depleted medium, suggesting a slight level of redundancy between <jats:italic>sodA1</jats:italic> and <jats:italic>sodA2</jats:italic> , and a role for the <jats:italic>sod</jats:italic> genes in manganese homeostasis. </jats:p> The Global Transcriptional Responses of <i>Bacillus anthracis</i> Sterne (34F <sub>2</sub> ) and a Δ <i>sodA1</i> Mutant to Paraquat Reveal Metal Ion Homeostasis Imbalances during Endogenous Superoxide Stress Journal of Bacteriology |
| spellingShingle | Passalacqua, Karla D., Bergman, Nicholas H., Lee, Jung Yeop, Sherman, David H., Hanna, Philip C., Journal of Bacteriology, The Global Transcriptional Responses of Bacillus anthracis Sterne (34F 2 ) and a Δ sodA1 Mutant to Paraquat Reveal Metal Ion Homeostasis Imbalances during Endogenous Superoxide Stress, Molecular Biology, Microbiology |
| title | The Global Transcriptional Responses of Bacillus anthracis Sterne (34F 2 ) and a Δ sodA1 Mutant to Paraquat Reveal Metal Ion Homeostasis Imbalances during Endogenous Superoxide Stress |
| title_full | The Global Transcriptional Responses of Bacillus anthracis Sterne (34F 2 ) and a Δ sodA1 Mutant to Paraquat Reveal Metal Ion Homeostasis Imbalances during Endogenous Superoxide Stress |
| title_fullStr | The Global Transcriptional Responses of Bacillus anthracis Sterne (34F 2 ) and a Δ sodA1 Mutant to Paraquat Reveal Metal Ion Homeostasis Imbalances during Endogenous Superoxide Stress |
| title_full_unstemmed | The Global Transcriptional Responses of Bacillus anthracis Sterne (34F 2 ) and a Δ sodA1 Mutant to Paraquat Reveal Metal Ion Homeostasis Imbalances during Endogenous Superoxide Stress |
| title_short | The Global Transcriptional Responses of Bacillus anthracis Sterne (34F 2 ) and a Δ sodA1 Mutant to Paraquat Reveal Metal Ion Homeostasis Imbalances during Endogenous Superoxide Stress |
| title_sort | the global transcriptional responses of <i>bacillus anthracis</i> sterne (34f <sub>2</sub> ) and a δ <i>soda1</i> mutant to paraquat reveal metal ion homeostasis imbalances during endogenous superoxide stress |
| title_unstemmed | The Global Transcriptional Responses of Bacillus anthracis Sterne (34F 2 ) and a Δ sodA1 Mutant to Paraquat Reveal Metal Ion Homeostasis Imbalances during Endogenous Superoxide Stress |
| topic | Molecular Biology, Microbiology |
| url | http://dx.doi.org/10.1128/jb.00185-07 |