Use of a Recombinant Envelope Protein Subunit Antigen for Specific Serological Diagnosis of West Nile Virus Infection

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Bibliographische Detailangaben
Zeitschriftentitel:	Journal of Clinical Microbiology
Personen und Körperschaften:	Beasley, David W. C., Holbrook, Michael R., Travassos da Rosa, Amelia P. A., Coffey, Lark, Carrara, Anne-Sophie, Phillippi-Falkenstein, Kathrine, Bohm, Rudolf P., Ratterree, Marion S., Lillibridge, Kristy M., Ludwig, George V., Estrada-Franco, Jose, Weaver, Scott C., Tesh, Robert B., Shope, Robert E., Barrett, Alan D. T.
In:	Journal of Clinical Microbiology, 42, 2004, 6, S. 2759-2765
Format:	E-Article
Sprache:	Englisch
veröffentlicht:	American Society for Microbiology
Schlagwörter:	Microbiology (medical)

Details
Zusammenfassung:	<jats:title>ABSTRACT</jats:title><jats:p>Serological diagnosis of West Nile virus (WNV) infection is complicated by extensive antigenic cross-reactivity with other closely related flaviviruses, such as St. Louis encephalitis virus. Here we describe a recombinant, bacterially expressed antigen equivalent to structural domain III of the WNV envelope protein that has allowed clear discrimination of antibody responses to WNV from those against other related flaviviruses in indirect enzyme-linked immunosorbent assays using standardized control antisera and field-collected samples.</jats:p>
Umfang:	2759-2765
ISSN:	0095-1137 1098-660X
DOI:	10.1128/jcm.42.6.2759-2765.2004