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Truncated Hantavirus Nucleocapsid Proteins for Serotyping Hantaan, Seoul, and Dobrava Hantavirus Infections
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| Zeitschriftentitel: | Journal of Clinical Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , , , , |
| In: | Journal of Clinical Microbiology, 39, 2001, 7, S. 2397-2404 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Araki, Koichi Yoshimatsu, Kumiko Ogino, Michiko Ebihara, Hideki Lundkvist, Åke Kariwa, Hiroaki Takashima, Ikuo Arikawa, Jiro Araki, Koichi Yoshimatsu, Kumiko Ogino, Michiko Ebihara, Hideki Lundkvist, Åke Kariwa, Hiroaki Takashima, Ikuo Arikawa, Jiro |
|---|---|
| author |
Araki, Koichi Yoshimatsu, Kumiko Ogino, Michiko Ebihara, Hideki Lundkvist, Åke Kariwa, Hiroaki Takashima, Ikuo Arikawa, Jiro |
| spellingShingle |
Araki, Koichi Yoshimatsu, Kumiko Ogino, Michiko Ebihara, Hideki Lundkvist, Åke Kariwa, Hiroaki Takashima, Ikuo Arikawa, Jiro Journal of Clinical Microbiology Truncated Hantavirus Nucleocapsid Proteins for Serotyping Hantaan, Seoul, and Dobrava Hantavirus Infections Microbiology (medical) |
| author_sort |
araki, koichi |
| spelling |
Araki, Koichi Yoshimatsu, Kumiko Ogino, Michiko Ebihara, Hideki Lundkvist, Åke Kariwa, Hiroaki Takashima, Ikuo Arikawa, Jiro 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.39.7.2397-2404.2001 <jats:title>ABSTRACT</jats:title> <jats:p>Truncated recombinant nucleocapsid proteins (rNPs) of Hantaan virus (HTNV), Seoul virus (SEOV), and Dobrava virus (DOBV) were expressed by a baculovirus system. The truncated rNPs, which lacked 49 (rNP50) or 154 (rNP155) N-terminal amino acids of the NPs of HTNV, SEOV, and DOBV, were able to differentiate HTNV-, SEOV-, and DOBV-specific immune sera. Recombinant NP50s retained higher reactivities than rNP155s and were proven useful for enzyme-linked immunosorbent assay (ELISA). The ELISAs based on the rNP50s of HTNV, SEOV, and DOBV successfully differentiated three groups of patient sera, previously defined by neutralization tests: 17 with HTNV infection, 12 with SEOV infection, and 20 with DOBV infection. The entire rNP of Puumala virus (PUUV) distinguished PUUV infection from the other types of hantavirus infection. Serotyping with these rNP50s can be recommended as a rapid and efficient system for hantavirus diagnosis.</jats:p> Truncated Hantavirus Nucleocapsid Proteins for Serotyping Hantaan, Seoul, and Dobrava Hantavirus Infections Journal of Clinical Microbiology |
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| title |
Truncated Hantavirus Nucleocapsid Proteins for Serotyping Hantaan, Seoul, and Dobrava Hantavirus Infections |
| title_unstemmed |
Truncated Hantavirus Nucleocapsid Proteins for Serotyping Hantaan, Seoul, and Dobrava Hantavirus Infections |
| title_full |
Truncated Hantavirus Nucleocapsid Proteins for Serotyping Hantaan, Seoul, and Dobrava Hantavirus Infections |
| title_fullStr |
Truncated Hantavirus Nucleocapsid Proteins for Serotyping Hantaan, Seoul, and Dobrava Hantavirus Infections |
| title_full_unstemmed |
Truncated Hantavirus Nucleocapsid Proteins for Serotyping Hantaan, Seoul, and Dobrava Hantavirus Infections |
| title_short |
Truncated Hantavirus Nucleocapsid Proteins for Serotyping Hantaan, Seoul, and Dobrava Hantavirus Infections |
| title_sort |
truncated hantavirus nucleocapsid proteins for serotyping hantaan, seoul, and dobrava hantavirus infections |
| topic |
Microbiology (medical) |
| url |
http://dx.doi.org/10.1128/jcm.39.7.2397-2404.2001 |
| publishDate |
2001 |
| physical |
2397-2404 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>Truncated recombinant nucleocapsid proteins (rNPs) of Hantaan virus (HTNV), Seoul virus (SEOV), and Dobrava virus (DOBV) were expressed by a baculovirus system. The truncated rNPs, which lacked 49 (rNP50) or 154 (rNP155) N-terminal amino acids of the NPs of HTNV, SEOV, and DOBV, were able to differentiate HTNV-, SEOV-, and DOBV-specific immune sera. Recombinant NP50s retained higher reactivities than rNP155s and were proven useful for enzyme-linked immunosorbent assay (ELISA). The ELISAs based on the rNP50s of HTNV, SEOV, and DOBV successfully differentiated three groups of patient sera, previously defined by neutralization tests: 17 with HTNV infection, 12 with SEOV infection, and 20 with DOBV infection. The entire rNP of Puumala virus (PUUV) distinguished PUUV infection from the other types of hantavirus infection. Serotyping with these rNP50s can be recommended as a rapid and efficient system for hantavirus diagnosis.</jats:p> |
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| author | Araki, Koichi, Yoshimatsu, Kumiko, Ogino, Michiko, Ebihara, Hideki, Lundkvist, Åke, Kariwa, Hiroaki, Takashima, Ikuo, Arikawa, Jiro |
| author_facet | Araki, Koichi, Yoshimatsu, Kumiko, Ogino, Michiko, Ebihara, Hideki, Lundkvist, Åke, Kariwa, Hiroaki, Takashima, Ikuo, Arikawa, Jiro, Araki, Koichi, Yoshimatsu, Kumiko, Ogino, Michiko, Ebihara, Hideki, Lundkvist, Åke, Kariwa, Hiroaki, Takashima, Ikuo, Arikawa, Jiro |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p>Truncated recombinant nucleocapsid proteins (rNPs) of Hantaan virus (HTNV), Seoul virus (SEOV), and Dobrava virus (DOBV) were expressed by a baculovirus system. The truncated rNPs, which lacked 49 (rNP50) or 154 (rNP155) N-terminal amino acids of the NPs of HTNV, SEOV, and DOBV, were able to differentiate HTNV-, SEOV-, and DOBV-specific immune sera. Recombinant NP50s retained higher reactivities than rNP155s and were proven useful for enzyme-linked immunosorbent assay (ELISA). The ELISAs based on the rNP50s of HTNV, SEOV, and DOBV successfully differentiated three groups of patient sera, previously defined by neutralization tests: 17 with HTNV infection, 12 with SEOV infection, and 20 with DOBV infection. The entire rNP of Puumala virus (PUUV) distinguished PUUV infection from the other types of hantavirus infection. Serotyping with these rNP50s can be recommended as a rapid and efficient system for hantavirus diagnosis.</jats:p> |
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| imprint_str_mv | American Society for Microbiology, 2001 |
| institution | DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15 |
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| spelling | Araki, Koichi Yoshimatsu, Kumiko Ogino, Michiko Ebihara, Hideki Lundkvist, Åke Kariwa, Hiroaki Takashima, Ikuo Arikawa, Jiro 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.39.7.2397-2404.2001 <jats:title>ABSTRACT</jats:title> <jats:p>Truncated recombinant nucleocapsid proteins (rNPs) of Hantaan virus (HTNV), Seoul virus (SEOV), and Dobrava virus (DOBV) were expressed by a baculovirus system. The truncated rNPs, which lacked 49 (rNP50) or 154 (rNP155) N-terminal amino acids of the NPs of HTNV, SEOV, and DOBV, were able to differentiate HTNV-, SEOV-, and DOBV-specific immune sera. Recombinant NP50s retained higher reactivities than rNP155s and were proven useful for enzyme-linked immunosorbent assay (ELISA). The ELISAs based on the rNP50s of HTNV, SEOV, and DOBV successfully differentiated three groups of patient sera, previously defined by neutralization tests: 17 with HTNV infection, 12 with SEOV infection, and 20 with DOBV infection. The entire rNP of Puumala virus (PUUV) distinguished PUUV infection from the other types of hantavirus infection. Serotyping with these rNP50s can be recommended as a rapid and efficient system for hantavirus diagnosis.</jats:p> Truncated Hantavirus Nucleocapsid Proteins for Serotyping Hantaan, Seoul, and Dobrava Hantavirus Infections Journal of Clinical Microbiology |
| spellingShingle | Araki, Koichi, Yoshimatsu, Kumiko, Ogino, Michiko, Ebihara, Hideki, Lundkvist, Åke, Kariwa, Hiroaki, Takashima, Ikuo, Arikawa, Jiro, Journal of Clinical Microbiology, Truncated Hantavirus Nucleocapsid Proteins for Serotyping Hantaan, Seoul, and Dobrava Hantavirus Infections, Microbiology (medical) |
| title | Truncated Hantavirus Nucleocapsid Proteins for Serotyping Hantaan, Seoul, and Dobrava Hantavirus Infections |
| title_full | Truncated Hantavirus Nucleocapsid Proteins for Serotyping Hantaan, Seoul, and Dobrava Hantavirus Infections |
| title_fullStr | Truncated Hantavirus Nucleocapsid Proteins for Serotyping Hantaan, Seoul, and Dobrava Hantavirus Infections |
| title_full_unstemmed | Truncated Hantavirus Nucleocapsid Proteins for Serotyping Hantaan, Seoul, and Dobrava Hantavirus Infections |
| title_short | Truncated Hantavirus Nucleocapsid Proteins for Serotyping Hantaan, Seoul, and Dobrava Hantavirus Infections |
| title_sort | truncated hantavirus nucleocapsid proteins for serotyping hantaan, seoul, and dobrava hantavirus infections |
| title_unstemmed | Truncated Hantavirus Nucleocapsid Proteins for Serotyping Hantaan, Seoul, and Dobrava Hantavirus Infections |
| topic | Microbiology (medical) |
| url | http://dx.doi.org/10.1128/jcm.39.7.2397-2404.2001 |