Eintrag weiter verarbeiten
Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens
Gespeichert in:
| Zeitschriftentitel: | Journal of Clinical Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Journal of Clinical Microbiology, 28, 1990, 11, S. 2514-2519 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Collins, M T Kenefick, K B Sockett, D C Lambrecht, R S McDonald, J Jorgensen, J B Collins, M T Kenefick, K B Sockett, D C Lambrecht, R S McDonald, J Jorgensen, J B |
|---|---|
| author |
Collins, M T Kenefick, K B Sockett, D C Lambrecht, R S McDonald, J Jorgensen, J B |
| spellingShingle |
Collins, M T Kenefick, K B Sockett, D C Lambrecht, R S McDonald, J Jorgensen, J B Journal of Clinical Microbiology Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens Microbiology (medical) |
| author_sort |
collins, m t |
| spelling |
Collins, M T Kenefick, K B Sockett, D C Lambrecht, R S McDonald, J Jorgensen, J B 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.28.11.2514-2519.1990 <jats:p>A commercial radiometric medium, BACTEC 12B, was modified by addition of mycobactin, egg yolk suspension, and antibiotics (vancomycin, amphotericin B, and nalidixic acid). Decontaminated bovine fecal specimens were filter concentrated by using 3-microns-pore-size, 13-mm-diameter polycarbonate filters, and the entire filter was placed into the radiometric broth. Comparison of the radiometric technique with conventional methods on 603 cattle from 9 Mycobacterium paratuberculosis-infected herds found that of 75 positive specimens, the radiometric technique detected 92% while conventional methods detected 60% (P less than 0.0005). Only 3.9% of radiometric cultures were contaminated. To measure the effect of filter concentration of specimens on the detection rate, 5 cattle with minimal and 5 with moderate ileum histopathology were sampled weekly for 3 weeks. M. paratuberculosis was detected in 33.3% of nonfiltered specimens and 76.7% of filtered specimens (P less than 0.005). Detection rates were directly correlated with the severity of disease, and the advantage of specimen concentration was greatest on fecal specimens from cattle with low-grade infections. Detection times were also correlated with infection severity: 13.4 +/- 5.9 days with smear-positive specimens, 27.9 +/- 8.7 days with feces from cows with typical subclinical infections, and 38.7 +/- 3.8 days with fecal specimens from cows with low-grade infections. Use of a cocktail of vancomycin, amphotericin B, and nalidixic acid for selective suppression of nonmycobacterial contaminants was better than the commercial product PANTA (Becton Dickinson Microbiologic Systems, Towson, Md.) only when specimens contained very low numbers of M. paratuberculosis. Radiometric culture of filter-concentrated specimens generally doubled the number of positive fecal specimens detected over conventional methods, making it a useful tool for diagnosis and control of bovine paratuberculosis.</jats:p> Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens Journal of Clinical Microbiology |
| doi_str_mv |
10.1128/jcm.28.11.2514-2519.1990 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qY20uMjguMTEuMjUxNC0yNTE5LjE5OTA |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qY20uMjguMTEuMjUxNC0yNTE5LjE5OTA |
| institution |
DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Zi4 DE-Gla1 DE-15 DE-Pl11 DE-Rs1 DE-14 DE-105 DE-Ch1 DE-L229 |
| imprint |
American Society for Microbiology, 1990 |
| imprint_str_mv |
American Society for Microbiology, 1990 |
| issn |
1098-660X 0095-1137 |
| issn_str_mv |
1098-660X 0095-1137 |
| language |
English |
| mega_collection |
American Society for Microbiology (CrossRef) |
| match_str |
collins1990enhancedradiometricdetectionofmycobacteriumparatuberculosisbyusingfilterconcentratedbovinefecalspecimens |
| publishDateSort |
1990 |
| publisher |
American Society for Microbiology |
| recordtype |
ai |
| record_format |
ai |
| series |
Journal of Clinical Microbiology |
| source_id |
49 |
| title |
Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens |
| title_unstemmed |
Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens |
| title_full |
Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens |
| title_fullStr |
Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens |
| title_full_unstemmed |
Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens |
| title_short |
Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens |
| title_sort |
enhanced radiometric detection of mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens |
| topic |
Microbiology (medical) |
| url |
http://dx.doi.org/10.1128/jcm.28.11.2514-2519.1990 |
| publishDate |
1990 |
| physical |
2514-2519 |
| description |
<jats:p>A commercial radiometric medium, BACTEC 12B, was modified by addition of mycobactin, egg yolk suspension, and antibiotics (vancomycin, amphotericin B, and nalidixic acid). Decontaminated bovine fecal specimens were filter concentrated by using 3-microns-pore-size, 13-mm-diameter polycarbonate filters, and the entire filter was placed into the radiometric broth. Comparison of the radiometric technique with conventional methods on 603 cattle from 9 Mycobacterium paratuberculosis-infected herds found that of 75 positive specimens, the radiometric technique detected 92% while conventional methods detected 60% (P less than 0.0005). Only 3.9% of radiometric cultures were contaminated. To measure the effect of filter concentration of specimens on the detection rate, 5 cattle with minimal and 5 with moderate ileum histopathology were sampled weekly for 3 weeks. M. paratuberculosis was detected in 33.3% of nonfiltered specimens and 76.7% of filtered specimens (P less than 0.005). Detection rates were directly correlated with the severity of disease, and the advantage of specimen concentration was greatest on fecal specimens from cattle with low-grade infections. Detection times were also correlated with infection severity: 13.4 +/- 5.9 days with smear-positive specimens, 27.9 +/- 8.7 days with feces from cows with typical subclinical infections, and 38.7 +/- 3.8 days with fecal specimens from cows with low-grade infections. Use of a cocktail of vancomycin, amphotericin B, and nalidixic acid for selective suppression of nonmycobacterial contaminants was better than the commercial product PANTA (Becton Dickinson Microbiologic Systems, Towson, Md.) only when specimens contained very low numbers of M. paratuberculosis. Radiometric culture of filter-concentrated specimens generally doubled the number of positive fecal specimens detected over conventional methods, making it a useful tool for diagnosis and control of bovine paratuberculosis.</jats:p> |
| container_issue |
11 |
| container_start_page |
2514 |
| container_title |
Journal of Clinical Microbiology |
| container_volume |
28 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792342870366093318 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T16:42:16.954Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Enhanced+radiometric+detection+of+Mycobacterium+paratuberculosis+by+using+filter-concentrated+bovine+fecal+specimens&rft.date=1990-11-01&genre=article&issn=1098-660X&volume=28&issue=11&spage=2514&epage=2519&pages=2514-2519&jtitle=Journal+of+Clinical+Microbiology&atitle=Enhanced+radiometric+detection+of+Mycobacterium+paratuberculosis+by+using+filter-concentrated+bovine+fecal+specimens&aulast=Jorgensen&aufirst=J+B&rft_id=info%3Adoi%2F10.1128%2Fjcm.28.11.2514-2519.1990&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792342870366093318 |
| author | Collins, M T, Kenefick, K B, Sockett, D C, Lambrecht, R S, McDonald, J, Jorgensen, J B |
| author_facet | Collins, M T, Kenefick, K B, Sockett, D C, Lambrecht, R S, McDonald, J, Jorgensen, J B, Collins, M T, Kenefick, K B, Sockett, D C, Lambrecht, R S, McDonald, J, Jorgensen, J B |
| author_sort | collins, m t |
| container_issue | 11 |
| container_start_page | 2514 |
| container_title | Journal of Clinical Microbiology |
| container_volume | 28 |
| description | <jats:p>A commercial radiometric medium, BACTEC 12B, was modified by addition of mycobactin, egg yolk suspension, and antibiotics (vancomycin, amphotericin B, and nalidixic acid). Decontaminated bovine fecal specimens were filter concentrated by using 3-microns-pore-size, 13-mm-diameter polycarbonate filters, and the entire filter was placed into the radiometric broth. Comparison of the radiometric technique with conventional methods on 603 cattle from 9 Mycobacterium paratuberculosis-infected herds found that of 75 positive specimens, the radiometric technique detected 92% while conventional methods detected 60% (P less than 0.0005). Only 3.9% of radiometric cultures were contaminated. To measure the effect of filter concentration of specimens on the detection rate, 5 cattle with minimal and 5 with moderate ileum histopathology were sampled weekly for 3 weeks. M. paratuberculosis was detected in 33.3% of nonfiltered specimens and 76.7% of filtered specimens (P less than 0.005). Detection rates were directly correlated with the severity of disease, and the advantage of specimen concentration was greatest on fecal specimens from cattle with low-grade infections. Detection times were also correlated with infection severity: 13.4 +/- 5.9 days with smear-positive specimens, 27.9 +/- 8.7 days with feces from cows with typical subclinical infections, and 38.7 +/- 3.8 days with fecal specimens from cows with low-grade infections. Use of a cocktail of vancomycin, amphotericin B, and nalidixic acid for selective suppression of nonmycobacterial contaminants was better than the commercial product PANTA (Becton Dickinson Microbiologic Systems, Towson, Md.) only when specimens contained very low numbers of M. paratuberculosis. Radiometric culture of filter-concentrated specimens generally doubled the number of positive fecal specimens detected over conventional methods, making it a useful tool for diagnosis and control of bovine paratuberculosis.</jats:p> |
| doi_str_mv | 10.1128/jcm.28.11.2514-2519.1990 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9qY20uMjguMTEuMjUxNC0yNTE5LjE5OTA |
| imprint | American Society for Microbiology, 1990 |
| imprint_str_mv | American Society for Microbiology, 1990 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229 |
| issn | 1098-660X, 0095-1137 |
| issn_str_mv | 1098-660X, 0095-1137 |
| language | English |
| last_indexed | 2024-03-01T16:42:16.954Z |
| match_str | collins1990enhancedradiometricdetectionofmycobacteriumparatuberculosisbyusingfilterconcentratedbovinefecalspecimens |
| mega_collection | American Society for Microbiology (CrossRef) |
| physical | 2514-2519 |
| publishDate | 1990 |
| publishDateSort | 1990 |
| publisher | American Society for Microbiology |
| record_format | ai |
| recordtype | ai |
| series | Journal of Clinical Microbiology |
| source_id | 49 |
| spelling | Collins, M T Kenefick, K B Sockett, D C Lambrecht, R S McDonald, J Jorgensen, J B 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.28.11.2514-2519.1990 <jats:p>A commercial radiometric medium, BACTEC 12B, was modified by addition of mycobactin, egg yolk suspension, and antibiotics (vancomycin, amphotericin B, and nalidixic acid). Decontaminated bovine fecal specimens were filter concentrated by using 3-microns-pore-size, 13-mm-diameter polycarbonate filters, and the entire filter was placed into the radiometric broth. Comparison of the radiometric technique with conventional methods on 603 cattle from 9 Mycobacterium paratuberculosis-infected herds found that of 75 positive specimens, the radiometric technique detected 92% while conventional methods detected 60% (P less than 0.0005). Only 3.9% of radiometric cultures were contaminated. To measure the effect of filter concentration of specimens on the detection rate, 5 cattle with minimal and 5 with moderate ileum histopathology were sampled weekly for 3 weeks. M. paratuberculosis was detected in 33.3% of nonfiltered specimens and 76.7% of filtered specimens (P less than 0.005). Detection rates were directly correlated with the severity of disease, and the advantage of specimen concentration was greatest on fecal specimens from cattle with low-grade infections. Detection times were also correlated with infection severity: 13.4 +/- 5.9 days with smear-positive specimens, 27.9 +/- 8.7 days with feces from cows with typical subclinical infections, and 38.7 +/- 3.8 days with fecal specimens from cows with low-grade infections. Use of a cocktail of vancomycin, amphotericin B, and nalidixic acid for selective suppression of nonmycobacterial contaminants was better than the commercial product PANTA (Becton Dickinson Microbiologic Systems, Towson, Md.) only when specimens contained very low numbers of M. paratuberculosis. Radiometric culture of filter-concentrated specimens generally doubled the number of positive fecal specimens detected over conventional methods, making it a useful tool for diagnosis and control of bovine paratuberculosis.</jats:p> Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens Journal of Clinical Microbiology |
| spellingShingle | Collins, M T, Kenefick, K B, Sockett, D C, Lambrecht, R S, McDonald, J, Jorgensen, J B, Journal of Clinical Microbiology, Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens, Microbiology (medical) |
| title | Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens |
| title_full | Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens |
| title_fullStr | Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens |
| title_full_unstemmed | Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens |
| title_short | Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens |
| title_sort | enhanced radiometric detection of mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens |
| title_unstemmed | Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens |
| topic | Microbiology (medical) |
| url | http://dx.doi.org/10.1128/jcm.28.11.2514-2519.1990 |