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Evaluation of a Novel PCR-Based Assay for Detection and Identification of Chlamydia trachomatis Serovars in Cervical Specimens
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| Zeitschriftentitel: | Journal of Clinical Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , , , , , |
| In: | Journal of Clinical Microbiology, 45, 2007, 12, S. 3986-3991 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Quint, Koen Porras, Carolina Safaeian, Mahboobeh González, Paula Hildesheim, Allan Quint, Wim van Doorn, Leen-Jan Silva, Sandra Melchers, Willem Schiffman, Mark Rodríguez, Ana Cecilia Wacholder, Sholom Freer, Enrique Cortes, Bernal Herrero, Rolando Quint, Koen Porras, Carolina Safaeian, Mahboobeh González, Paula Hildesheim, Allan Quint, Wim van Doorn, Leen-Jan Silva, Sandra Melchers, Willem Schiffman, Mark Rodríguez, Ana Cecilia Wacholder, Sholom Freer, Enrique Cortes, Bernal Herrero, Rolando |
|---|---|
| author |
Quint, Koen Porras, Carolina Safaeian, Mahboobeh González, Paula Hildesheim, Allan Quint, Wim van Doorn, Leen-Jan Silva, Sandra Melchers, Willem Schiffman, Mark Rodríguez, Ana Cecilia Wacholder, Sholom Freer, Enrique Cortes, Bernal Herrero, Rolando |
| spellingShingle |
Quint, Koen Porras, Carolina Safaeian, Mahboobeh González, Paula Hildesheim, Allan Quint, Wim van Doorn, Leen-Jan Silva, Sandra Melchers, Willem Schiffman, Mark Rodríguez, Ana Cecilia Wacholder, Sholom Freer, Enrique Cortes, Bernal Herrero, Rolando Journal of Clinical Microbiology Evaluation of a Novel PCR-Based Assay for Detection and Identification of Chlamydia trachomatis Serovars in Cervical Specimens Microbiology (medical) |
| author_sort |
quint, koen |
| spelling |
Quint, Koen Porras, Carolina Safaeian, Mahboobeh González, Paula Hildesheim, Allan Quint, Wim van Doorn, Leen-Jan Silva, Sandra Melchers, Willem Schiffman, Mark Rodríguez, Ana Cecilia Wacholder, Sholom Freer, Enrique Cortes, Bernal Herrero, Rolando 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.01155-07 <jats:title>ABSTRACT</jats:title> <jats:p> The aims of this study were to compare a novel PCR-based <jats:italic>Chlamydia trachomatis</jats:italic> detection and genotyping (Ct-DT) assay with the FDA-approved, commercially available <jats:italic>C. trachomatis</jats:italic> detection Hybrid Capture 2 (HC2) assay and to investigate the <jats:italic>C. trachomatis</jats:italic> serovar distribution among young women in a rural Costa Rican study population. A total of 5,828 sexually active women participating in a community-based trial in Costa Rica were tested for <jats:italic>C. trachomatis</jats:italic> by HC2. A sample of 1,229 specimens consisting of 100% HC2 <jats:italic>C. trachomatis</jats:italic> -positive specimens ( <jats:italic>n</jats:italic> = 827) and a random sample of 8% HC2 <jats:italic>C. trachomatis</jats:italic> -negative specimens ( <jats:italic>n</jats:italic> = 402) were tested with the Ct-DT assay. Agreement between the two assays was determined by the unweighted kappa statistic. Discrepant specimens were tested with a second commercially available test (COBAS TaqMan). The Ct-DT-positive specimens were further analyzed with the Ct-DT genotyping step to investigate the distribution of 14 different <jats:italic>C. trachomatis</jats:italic> serovars (A, B/Ba, C, D/Da, E, F, G/Ga, H, I/Ia, J, K, L1, L2/L2a, and L3). After accounting for the sampling fraction selected for Ct-DT testing, crude agreement with the HC2 assay was 98% and the kappa was 0.92 (95% confidence interval [CI], 0.89 to 0.97). The 33 discordant samples that were further analyzed with the COBAS TaqMan test showed better agreement with the Ct-DT assay (31/33, <jats:italic>P</jats:italic> < 0.001). Among the 806 Ct-DT-positive samples, serovar E was the most common serovar (31%), followed by serovars F and D (both 21%) and serovar I (15%). In conclusion, the novel Ct-DT assay permits reliable detection and identification of <jats:italic>C. trachomatis</jats:italic> serovars. </jats:p> Evaluation of a Novel PCR-Based Assay for Detection and Identification of <i>Chlamydia trachomatis</i> Serovars in Cervical Specimens Journal of Clinical Microbiology |
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American Society for Microbiology, 2007 |
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| title |
Evaluation of a Novel PCR-Based Assay for Detection and Identification of Chlamydia trachomatis Serovars in Cervical Specimens |
| title_unstemmed |
Evaluation of a Novel PCR-Based Assay for Detection and Identification of Chlamydia trachomatis Serovars in Cervical Specimens |
| title_full |
Evaluation of a Novel PCR-Based Assay for Detection and Identification of Chlamydia trachomatis Serovars in Cervical Specimens |
| title_fullStr |
Evaluation of a Novel PCR-Based Assay for Detection and Identification of Chlamydia trachomatis Serovars in Cervical Specimens |
| title_full_unstemmed |
Evaluation of a Novel PCR-Based Assay for Detection and Identification of Chlamydia trachomatis Serovars in Cervical Specimens |
| title_short |
Evaluation of a Novel PCR-Based Assay for Detection and Identification of Chlamydia trachomatis Serovars in Cervical Specimens |
| title_sort |
evaluation of a novel pcr-based assay for detection and identification of
<i>chlamydia trachomatis</i>
serovars in cervical specimens |
| topic |
Microbiology (medical) |
| url |
http://dx.doi.org/10.1128/jcm.01155-07 |
| publishDate |
2007 |
| physical |
3986-3991 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
The aims of this study were to compare a novel PCR-based
<jats:italic>Chlamydia trachomatis</jats:italic>
detection and genotyping (Ct-DT) assay with the FDA-approved, commercially available
<jats:italic>C. trachomatis</jats:italic>
detection Hybrid Capture 2 (HC2) assay and to investigate the
<jats:italic>C. trachomatis</jats:italic>
serovar distribution among young women in a rural Costa Rican study population. A total of 5,828 sexually active women participating in a community-based trial in Costa Rica were tested for
<jats:italic>C. trachomatis</jats:italic>
by HC2. A sample of 1,229 specimens consisting of 100% HC2
<jats:italic>C. trachomatis</jats:italic>
-positive specimens (
<jats:italic>n</jats:italic>
= 827) and a random sample of 8% HC2
<jats:italic>C. trachomatis</jats:italic>
-negative specimens (
<jats:italic>n</jats:italic>
= 402) were tested with the Ct-DT assay. Agreement between the two assays was determined by the unweighted kappa statistic. Discrepant specimens were tested with a second commercially available test (COBAS TaqMan). The Ct-DT-positive specimens were further analyzed with the Ct-DT genotyping step to investigate the distribution of 14 different
<jats:italic>C. trachomatis</jats:italic>
serovars (A, B/Ba, C, D/Da, E, F, G/Ga, H, I/Ia, J, K, L1, L2/L2a, and L3). After accounting for the sampling fraction selected for Ct-DT testing, crude agreement with the HC2 assay was 98% and the kappa was 0.92 (95% confidence interval [CI], 0.89 to 0.97). The 33 discordant samples that were further analyzed with the COBAS TaqMan test showed better agreement with the Ct-DT assay (31/33,
<jats:italic>P</jats:italic>
< 0.001). Among the 806 Ct-DT-positive samples, serovar E was the most common serovar (31%), followed by serovars F and D (both 21%) and serovar I (15%). In conclusion, the novel Ct-DT assay permits reliable detection and identification of
<jats:italic>C. trachomatis</jats:italic>
serovars.
</jats:p> |
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| author | Quint, Koen, Porras, Carolina, Safaeian, Mahboobeh, González, Paula, Hildesheim, Allan, Quint, Wim, van Doorn, Leen-Jan, Silva, Sandra, Melchers, Willem, Schiffman, Mark, Rodríguez, Ana Cecilia, Wacholder, Sholom, Freer, Enrique, Cortes, Bernal, Herrero, Rolando |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> The aims of this study were to compare a novel PCR-based <jats:italic>Chlamydia trachomatis</jats:italic> detection and genotyping (Ct-DT) assay with the FDA-approved, commercially available <jats:italic>C. trachomatis</jats:italic> detection Hybrid Capture 2 (HC2) assay and to investigate the <jats:italic>C. trachomatis</jats:italic> serovar distribution among young women in a rural Costa Rican study population. A total of 5,828 sexually active women participating in a community-based trial in Costa Rica were tested for <jats:italic>C. trachomatis</jats:italic> by HC2. A sample of 1,229 specimens consisting of 100% HC2 <jats:italic>C. trachomatis</jats:italic> -positive specimens ( <jats:italic>n</jats:italic> = 827) and a random sample of 8% HC2 <jats:italic>C. trachomatis</jats:italic> -negative specimens ( <jats:italic>n</jats:italic> = 402) were tested with the Ct-DT assay. Agreement between the two assays was determined by the unweighted kappa statistic. Discrepant specimens were tested with a second commercially available test (COBAS TaqMan). The Ct-DT-positive specimens were further analyzed with the Ct-DT genotyping step to investigate the distribution of 14 different <jats:italic>C. trachomatis</jats:italic> serovars (A, B/Ba, C, D/Da, E, F, G/Ga, H, I/Ia, J, K, L1, L2/L2a, and L3). After accounting for the sampling fraction selected for Ct-DT testing, crude agreement with the HC2 assay was 98% and the kappa was 0.92 (95% confidence interval [CI], 0.89 to 0.97). The 33 discordant samples that were further analyzed with the COBAS TaqMan test showed better agreement with the Ct-DT assay (31/33, <jats:italic>P</jats:italic> < 0.001). Among the 806 Ct-DT-positive samples, serovar E was the most common serovar (31%), followed by serovars F and D (both 21%) and serovar I (15%). In conclusion, the novel Ct-DT assay permits reliable detection and identification of <jats:italic>C. trachomatis</jats:italic> serovars. </jats:p> |
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| spelling | Quint, Koen Porras, Carolina Safaeian, Mahboobeh González, Paula Hildesheim, Allan Quint, Wim van Doorn, Leen-Jan Silva, Sandra Melchers, Willem Schiffman, Mark Rodríguez, Ana Cecilia Wacholder, Sholom Freer, Enrique Cortes, Bernal Herrero, Rolando 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.01155-07 <jats:title>ABSTRACT</jats:title> <jats:p> The aims of this study were to compare a novel PCR-based <jats:italic>Chlamydia trachomatis</jats:italic> detection and genotyping (Ct-DT) assay with the FDA-approved, commercially available <jats:italic>C. trachomatis</jats:italic> detection Hybrid Capture 2 (HC2) assay and to investigate the <jats:italic>C. trachomatis</jats:italic> serovar distribution among young women in a rural Costa Rican study population. A total of 5,828 sexually active women participating in a community-based trial in Costa Rica were tested for <jats:italic>C. trachomatis</jats:italic> by HC2. A sample of 1,229 specimens consisting of 100% HC2 <jats:italic>C. trachomatis</jats:italic> -positive specimens ( <jats:italic>n</jats:italic> = 827) and a random sample of 8% HC2 <jats:italic>C. trachomatis</jats:italic> -negative specimens ( <jats:italic>n</jats:italic> = 402) were tested with the Ct-DT assay. Agreement between the two assays was determined by the unweighted kappa statistic. Discrepant specimens were tested with a second commercially available test (COBAS TaqMan). The Ct-DT-positive specimens were further analyzed with the Ct-DT genotyping step to investigate the distribution of 14 different <jats:italic>C. trachomatis</jats:italic> serovars (A, B/Ba, C, D/Da, E, F, G/Ga, H, I/Ia, J, K, L1, L2/L2a, and L3). After accounting for the sampling fraction selected for Ct-DT testing, crude agreement with the HC2 assay was 98% and the kappa was 0.92 (95% confidence interval [CI], 0.89 to 0.97). The 33 discordant samples that were further analyzed with the COBAS TaqMan test showed better agreement with the Ct-DT assay (31/33, <jats:italic>P</jats:italic> < 0.001). Among the 806 Ct-DT-positive samples, serovar E was the most common serovar (31%), followed by serovars F and D (both 21%) and serovar I (15%). In conclusion, the novel Ct-DT assay permits reliable detection and identification of <jats:italic>C. trachomatis</jats:italic> serovars. </jats:p> Evaluation of a Novel PCR-Based Assay for Detection and Identification of <i>Chlamydia trachomatis</i> Serovars in Cervical Specimens Journal of Clinical Microbiology |
| spellingShingle | Quint, Koen, Porras, Carolina, Safaeian, Mahboobeh, González, Paula, Hildesheim, Allan, Quint, Wim, van Doorn, Leen-Jan, Silva, Sandra, Melchers, Willem, Schiffman, Mark, Rodríguez, Ana Cecilia, Wacholder, Sholom, Freer, Enrique, Cortes, Bernal, Herrero, Rolando, Journal of Clinical Microbiology, Evaluation of a Novel PCR-Based Assay for Detection and Identification of Chlamydia trachomatis Serovars in Cervical Specimens, Microbiology (medical) |
| title | Evaluation of a Novel PCR-Based Assay for Detection and Identification of Chlamydia trachomatis Serovars in Cervical Specimens |
| title_full | Evaluation of a Novel PCR-Based Assay for Detection and Identification of Chlamydia trachomatis Serovars in Cervical Specimens |
| title_fullStr | Evaluation of a Novel PCR-Based Assay for Detection and Identification of Chlamydia trachomatis Serovars in Cervical Specimens |
| title_full_unstemmed | Evaluation of a Novel PCR-Based Assay for Detection and Identification of Chlamydia trachomatis Serovars in Cervical Specimens |
| title_short | Evaluation of a Novel PCR-Based Assay for Detection and Identification of Chlamydia trachomatis Serovars in Cervical Specimens |
| title_sort | evaluation of a novel pcr-based assay for detection and identification of <i>chlamydia trachomatis</i> serovars in cervical specimens |
| title_unstemmed | Evaluation of a Novel PCR-Based Assay for Detection and Identification of Chlamydia trachomatis Serovars in Cervical Specimens |
| topic | Microbiology (medical) |
| url | http://dx.doi.org/10.1128/jcm.01155-07 |