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Quantitation of Cytomegalovirus DNA Load in Dried Blood Spots Correlates Well with Plasma Viral Load
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| Zeitschriftentitel: | Journal of Clinical Microbiology |
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| Personen und Körperschaften: | , , , , , |
| In: | Journal of Clinical Microbiology, 51, 2013, 7, S. 2360-2364 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| author_facet |
Limaye, Ajit P. Santo Hayes, Tracy K. Huang, Meei-Li Magaret, Amalia Boeckh, Michael Jerome, Keith R. Limaye, Ajit P. Santo Hayes, Tracy K. Huang, Meei-Li Magaret, Amalia Boeckh, Michael Jerome, Keith R. |
|---|---|
| author |
Limaye, Ajit P. Santo Hayes, Tracy K. Huang, Meei-Li Magaret, Amalia Boeckh, Michael Jerome, Keith R. |
| spellingShingle |
Limaye, Ajit P. Santo Hayes, Tracy K. Huang, Meei-Li Magaret, Amalia Boeckh, Michael Jerome, Keith R. Journal of Clinical Microbiology Quantitation of Cytomegalovirus DNA Load in Dried Blood Spots Correlates Well with Plasma Viral Load Microbiology (medical) |
| author_sort |
limaye, ajit p. |
| spelling |
Limaye, Ajit P. Santo Hayes, Tracy K. Huang, Meei-Li Magaret, Amalia Boeckh, Michael Jerome, Keith R. 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.00316-13 <jats:title>ABSTRACT</jats:title> <jats:p> An assay to accurately quantitate cytomegalovirus (CMV) load in finger-stick-collected dried blood spots (DBS) could potentially be useful for field studies or for analyzing patient self-collected specimens. We therefore assessed CMV DNA load in paired venipuncture-collected plasma samples and finger-stick DBS, using a previously validated quantitative PCR assay. Assay variability, sensitivity, and changes in viral load during antiviral therapy in finger-stick DBS were compared to the reference plasma quantitative PCR assay, using 106 prospectively collected pairs of finger-stick DBS and plasma samples from 35 solid-organ transplant (SOT) patients. The DBS assay showed good agreement with the reference plasma viral load assay on the log <jats:sub>10</jats:sub> scale (Pearson correlation coefficient, 0.92; <jats:italic>P</jats:italic> < 0.001). The 95% limit of detection of the DBS assay was estimated at 2,700 plasma copies/ml (675 plasma IU/ml). In 94% (76/81) of paired DBS and plasma samples above the limit of detection, the difference in CMV load was <1 log <jats:sub>10</jats:sub> . CMV viral load changes during antiviral treatment were comparable in plasma and DBS. We conclude that finger-stick DBS provides a convenient sample type for quantitation of CMV load that correlates well with plasma levels. Future studies to optimize and evaluate this methodology for patient self-collected samples are warranted. </jats:p> Quantitation of Cytomegalovirus DNA Load in Dried Blood Spots Correlates Well with Plasma Viral Load Journal of Clinical Microbiology |
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American Society for Microbiology, 2013 |
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American Society for Microbiology, 2013 |
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Journal of Clinical Microbiology |
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| title |
Quantitation of Cytomegalovirus DNA Load in Dried Blood Spots Correlates Well with Plasma Viral Load |
| title_unstemmed |
Quantitation of Cytomegalovirus DNA Load in Dried Blood Spots Correlates Well with Plasma Viral Load |
| title_full |
Quantitation of Cytomegalovirus DNA Load in Dried Blood Spots Correlates Well with Plasma Viral Load |
| title_fullStr |
Quantitation of Cytomegalovirus DNA Load in Dried Blood Spots Correlates Well with Plasma Viral Load |
| title_full_unstemmed |
Quantitation of Cytomegalovirus DNA Load in Dried Blood Spots Correlates Well with Plasma Viral Load |
| title_short |
Quantitation of Cytomegalovirus DNA Load in Dried Blood Spots Correlates Well with Plasma Viral Load |
| title_sort |
quantitation of cytomegalovirus dna load in dried blood spots correlates well with plasma viral load |
| topic |
Microbiology (medical) |
| url |
http://dx.doi.org/10.1128/jcm.00316-13 |
| publishDate |
2013 |
| physical |
2360-2364 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
An assay to accurately quantitate cytomegalovirus (CMV) load in finger-stick-collected dried blood spots (DBS) could potentially be useful for field studies or for analyzing patient self-collected specimens. We therefore assessed CMV DNA load in paired venipuncture-collected plasma samples and finger-stick DBS, using a previously validated quantitative PCR assay. Assay variability, sensitivity, and changes in viral load during antiviral therapy in finger-stick DBS were compared to the reference plasma quantitative PCR assay, using 106 prospectively collected pairs of finger-stick DBS and plasma samples from 35 solid-organ transplant (SOT) patients. The DBS assay showed good agreement with the reference plasma viral load assay on the log
<jats:sub>10</jats:sub>
scale (Pearson correlation coefficient, 0.92;
<jats:italic>P</jats:italic>
< 0.001). The 95% limit of detection of the DBS assay was estimated at 2,700 plasma copies/ml (675 plasma IU/ml). In 94% (76/81) of paired DBS and plasma samples above the limit of detection, the difference in CMV load was <1 log
<jats:sub>10</jats:sub>
. CMV viral load changes during antiviral treatment were comparable in plasma and DBS. We conclude that finger-stick DBS provides a convenient sample type for quantitation of CMV load that correlates well with plasma levels. Future studies to optimize and evaluate this methodology for patient self-collected samples are warranted.
</jats:p> |
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| author | Limaye, Ajit P., Santo Hayes, Tracy K., Huang, Meei-Li, Magaret, Amalia, Boeckh, Michael, Jerome, Keith R. |
| author_facet | Limaye, Ajit P., Santo Hayes, Tracy K., Huang, Meei-Li, Magaret, Amalia, Boeckh, Michael, Jerome, Keith R., Limaye, Ajit P., Santo Hayes, Tracy K., Huang, Meei-Li, Magaret, Amalia, Boeckh, Michael, Jerome, Keith R. |
| author_sort | limaye, ajit p. |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> An assay to accurately quantitate cytomegalovirus (CMV) load in finger-stick-collected dried blood spots (DBS) could potentially be useful for field studies or for analyzing patient self-collected specimens. We therefore assessed CMV DNA load in paired venipuncture-collected plasma samples and finger-stick DBS, using a previously validated quantitative PCR assay. Assay variability, sensitivity, and changes in viral load during antiviral therapy in finger-stick DBS were compared to the reference plasma quantitative PCR assay, using 106 prospectively collected pairs of finger-stick DBS and plasma samples from 35 solid-organ transplant (SOT) patients. The DBS assay showed good agreement with the reference plasma viral load assay on the log <jats:sub>10</jats:sub> scale (Pearson correlation coefficient, 0.92; <jats:italic>P</jats:italic> < 0.001). The 95% limit of detection of the DBS assay was estimated at 2,700 plasma copies/ml (675 plasma IU/ml). In 94% (76/81) of paired DBS and plasma samples above the limit of detection, the difference in CMV load was <1 log <jats:sub>10</jats:sub> . CMV viral load changes during antiviral treatment were comparable in plasma and DBS. We conclude that finger-stick DBS provides a convenient sample type for quantitation of CMV load that correlates well with plasma levels. Future studies to optimize and evaluate this methodology for patient self-collected samples are warranted. </jats:p> |
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| imprint_str_mv | American Society for Microbiology, 2013 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11, DE-105, DE-14, DE-Ch1, DE-L229 |
| issn | 1098-660X, 0095-1137 |
| issn_str_mv | 1098-660X, 0095-1137 |
| language | English |
| last_indexed | 2024-03-01T14:28:32.74Z |
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| physical | 2360-2364 |
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| spelling | Limaye, Ajit P. Santo Hayes, Tracy K. Huang, Meei-Li Magaret, Amalia Boeckh, Michael Jerome, Keith R. 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.00316-13 <jats:title>ABSTRACT</jats:title> <jats:p> An assay to accurately quantitate cytomegalovirus (CMV) load in finger-stick-collected dried blood spots (DBS) could potentially be useful for field studies or for analyzing patient self-collected specimens. We therefore assessed CMV DNA load in paired venipuncture-collected plasma samples and finger-stick DBS, using a previously validated quantitative PCR assay. Assay variability, sensitivity, and changes in viral load during antiviral therapy in finger-stick DBS were compared to the reference plasma quantitative PCR assay, using 106 prospectively collected pairs of finger-stick DBS and plasma samples from 35 solid-organ transplant (SOT) patients. The DBS assay showed good agreement with the reference plasma viral load assay on the log <jats:sub>10</jats:sub> scale (Pearson correlation coefficient, 0.92; <jats:italic>P</jats:italic> < 0.001). The 95% limit of detection of the DBS assay was estimated at 2,700 plasma copies/ml (675 plasma IU/ml). In 94% (76/81) of paired DBS and plasma samples above the limit of detection, the difference in CMV load was <1 log <jats:sub>10</jats:sub> . CMV viral load changes during antiviral treatment were comparable in plasma and DBS. We conclude that finger-stick DBS provides a convenient sample type for quantitation of CMV load that correlates well with plasma levels. Future studies to optimize and evaluate this methodology for patient self-collected samples are warranted. </jats:p> Quantitation of Cytomegalovirus DNA Load in Dried Blood Spots Correlates Well with Plasma Viral Load Journal of Clinical Microbiology |
| spellingShingle | Limaye, Ajit P., Santo Hayes, Tracy K., Huang, Meei-Li, Magaret, Amalia, Boeckh, Michael, Jerome, Keith R., Journal of Clinical Microbiology, Quantitation of Cytomegalovirus DNA Load in Dried Blood Spots Correlates Well with Plasma Viral Load, Microbiology (medical) |
| title | Quantitation of Cytomegalovirus DNA Load in Dried Blood Spots Correlates Well with Plasma Viral Load |
| title_full | Quantitation of Cytomegalovirus DNA Load in Dried Blood Spots Correlates Well with Plasma Viral Load |
| title_fullStr | Quantitation of Cytomegalovirus DNA Load in Dried Blood Spots Correlates Well with Plasma Viral Load |
| title_full_unstemmed | Quantitation of Cytomegalovirus DNA Load in Dried Blood Spots Correlates Well with Plasma Viral Load |
| title_short | Quantitation of Cytomegalovirus DNA Load in Dried Blood Spots Correlates Well with Plasma Viral Load |
| title_sort | quantitation of cytomegalovirus dna load in dried blood spots correlates well with plasma viral load |
| title_unstemmed | Quantitation of Cytomegalovirus DNA Load in Dried Blood Spots Correlates Well with Plasma Viral Load |
| topic | Microbiology (medical) |
| url | http://dx.doi.org/10.1128/jcm.00316-13 |