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Evaluation of Double-Disk Potentiation and Disk Potentiation Tests Using Dipicolinic Acid for Detection of Metallo-β-Lactamase-Producing Pseudomonas spp. and Acinetobacter spp
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| Zeitschriftentitel: | Journal of Clinical Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Journal of Clinical Microbiology, 50, 2012, 10, S. 3227-3232 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Yong, Dongeun Lee, Yangsoon Jeong, Seok Hoon Lee, Kyungwon Chong, Yunsop Yong, Dongeun Lee, Yangsoon Jeong, Seok Hoon Lee, Kyungwon Chong, Yunsop |
|---|---|
| author |
Yong, Dongeun Lee, Yangsoon Jeong, Seok Hoon Lee, Kyungwon Chong, Yunsop |
| spellingShingle |
Yong, Dongeun Lee, Yangsoon Jeong, Seok Hoon Lee, Kyungwon Chong, Yunsop Journal of Clinical Microbiology Evaluation of Double-Disk Potentiation and Disk Potentiation Tests Using Dipicolinic Acid for Detection of Metallo-β-Lactamase-Producing Pseudomonas spp. and Acinetobacter spp Microbiology (medical) |
| author_sort |
yong, dongeun |
| spelling |
Yong, Dongeun Lee, Yangsoon Jeong, Seok Hoon Lee, Kyungwon Chong, Yunsop 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.00818-12 <jats:title>ABSTRACT</jats:title> <jats:p> Accurate detection of metallo-β-lactamase (MBL)-producing <jats:named-content content-type="genus-species">Pseudomonas</jats:named-content> spp. and <jats:named-content content-type="genus-species">Acinetobacter</jats:named-content> spp. became very important with the increasing prevalence of carbapenem-nonsusceptible clinical isolates. The performance of phenotypic MBL detection methods may depend on the types of MBL and the characteristics of the isolates. A high false-positive rate is a problem with EDTA-based MBL detection methods. We evaluated the performance of double-disk potentiation tests (DDPTs) and disk potentiation tests (DPTs) with dipicolinic acid (DPA) using 44 isolates of <jats:named-content content-type="genus-species">Pseudomonas</jats:named-content> spp. and <jats:named-content content-type="genus-species">Acinetobacter</jats:named-content> spp. producing IMP-1-like, VIM-2-like, and SIM-1 type MBLs. Also, we characterized <jats:named-content content-type="genus-species">P. aeruginosa</jats:named-content> isolates with positive imipenem (IPM)-DPA DDPT, but negative meropenem (MEM)-DPA DDPT, and determined possibility of improving a DDPT by using MacConkey agar. Among five different DDPT methods, the IPM-DPA 250-μg method showed the highest sensitivity (97.7%) and specificity (100%). Among four DPT tests, the highest sensitivity (100%) was shown by the IPM-EDTA 1,900-μg disk method, but the specificity was very low (11.4%). Five of six <jats:named-content content-type="genus-species">P. aeruginosa</jats:named-content> isolates with false-negative DDPTs with MEM-DPA 250-μg disks carried <jats:italic>bla</jats:italic> <jats:sub>IMP-6,</jats:sub> and the high level resistance to MEM (MIC ≥ 512 μg/ml) was reduced by the presence of phenylalanine arginine β-naphtylamide. Improvement of DDPTs was observed when MacConkey agar was used instead of Mueller-Hinton agar. In conclusion, DPA is a better MBL inhibitor than EDTA for detection of <jats:named-content content-type="genus-species">Pseudomonas</jats:named-content> spp. and <jats:named-content content-type="genus-species">Acinetobacter</jats:named-content> spp. with IMP-1-like, VIM-2-like, and SIM-1-type MBLs. In DPA DDPTs, IPM disks perform better than MEM disks when the isolates are highly resistant to MEM due to the overexpression of efflux pumps. </jats:p> Evaluation of Double-Disk Potentiation and Disk Potentiation Tests Using Dipicolinic Acid for Detection of Metallo-β-Lactamase-Producing Pseudomonas spp. and Acinetobacter spp Journal of Clinical Microbiology |
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10.1128/jcm.00818-12 |
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Biologie |
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American Society for Microbiology, 2012 |
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American Society for Microbiology, 2012 |
| issn |
0095-1137 1098-660X |
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2012 |
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American Society for Microbiology |
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Journal of Clinical Microbiology |
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49 |
| title |
Evaluation of Double-Disk Potentiation and Disk Potentiation Tests Using Dipicolinic Acid for Detection of Metallo-β-Lactamase-Producing Pseudomonas spp. and Acinetobacter spp |
| title_unstemmed |
Evaluation of Double-Disk Potentiation and Disk Potentiation Tests Using Dipicolinic Acid for Detection of Metallo-β-Lactamase-Producing Pseudomonas spp. and Acinetobacter spp |
| title_full |
Evaluation of Double-Disk Potentiation and Disk Potentiation Tests Using Dipicolinic Acid for Detection of Metallo-β-Lactamase-Producing Pseudomonas spp. and Acinetobacter spp |
| title_fullStr |
Evaluation of Double-Disk Potentiation and Disk Potentiation Tests Using Dipicolinic Acid for Detection of Metallo-β-Lactamase-Producing Pseudomonas spp. and Acinetobacter spp |
| title_full_unstemmed |
Evaluation of Double-Disk Potentiation and Disk Potentiation Tests Using Dipicolinic Acid for Detection of Metallo-β-Lactamase-Producing Pseudomonas spp. and Acinetobacter spp |
| title_short |
Evaluation of Double-Disk Potentiation and Disk Potentiation Tests Using Dipicolinic Acid for Detection of Metallo-β-Lactamase-Producing Pseudomonas spp. and Acinetobacter spp |
| title_sort |
evaluation of double-disk potentiation and disk potentiation tests using dipicolinic acid for detection of metallo-β-lactamase-producing pseudomonas spp. and acinetobacter spp |
| topic |
Microbiology (medical) |
| url |
http://dx.doi.org/10.1128/jcm.00818-12 |
| publishDate |
2012 |
| physical |
3227-3232 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Accurate detection of metallo-β-lactamase (MBL)-producing
<jats:named-content content-type="genus-species">Pseudomonas</jats:named-content>
spp. and
<jats:named-content content-type="genus-species">Acinetobacter</jats:named-content>
spp. became very important with the increasing prevalence of carbapenem-nonsusceptible clinical isolates. The performance of phenotypic MBL detection methods may depend on the types of MBL and the characteristics of the isolates. A high false-positive rate is a problem with EDTA-based MBL detection methods. We evaluated the performance of double-disk potentiation tests (DDPTs) and disk potentiation tests (DPTs) with dipicolinic acid (DPA) using 44 isolates of
<jats:named-content content-type="genus-species">Pseudomonas</jats:named-content>
spp. and
<jats:named-content content-type="genus-species">Acinetobacter</jats:named-content>
spp. producing IMP-1-like, VIM-2-like, and SIM-1 type MBLs. Also, we characterized
<jats:named-content content-type="genus-species">P. aeruginosa</jats:named-content>
isolates with positive imipenem (IPM)-DPA DDPT, but negative meropenem (MEM)-DPA DDPT, and determined possibility of improving a DDPT by using MacConkey agar. Among five different DDPT methods, the IPM-DPA 250-μg method showed the highest sensitivity (97.7%) and specificity (100%). Among four DPT tests, the highest sensitivity (100%) was shown by the IPM-EDTA 1,900-μg disk method, but the specificity was very low (11.4%). Five of six
<jats:named-content content-type="genus-species">P. aeruginosa</jats:named-content>
isolates with false-negative DDPTs with MEM-DPA 250-μg disks carried
<jats:italic>bla</jats:italic>
<jats:sub>IMP-6,</jats:sub>
and the high level resistance to MEM (MIC ≥ 512 μg/ml) was reduced by the presence of phenylalanine arginine β-naphtylamide. Improvement of DDPTs was observed when MacConkey agar was used instead of Mueller-Hinton agar. In conclusion, DPA is a better MBL inhibitor than EDTA for detection of
<jats:named-content content-type="genus-species">Pseudomonas</jats:named-content>
spp. and
<jats:named-content content-type="genus-species">Acinetobacter</jats:named-content>
spp. with IMP-1-like, VIM-2-like, and SIM-1-type MBLs. In DPA DDPTs, IPM disks perform better than MEM disks when the isolates are highly resistant to MEM due to the overexpression of efflux pumps.
</jats:p> |
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| author | Yong, Dongeun, Lee, Yangsoon, Jeong, Seok Hoon, Lee, Kyungwon, Chong, Yunsop |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> Accurate detection of metallo-β-lactamase (MBL)-producing <jats:named-content content-type="genus-species">Pseudomonas</jats:named-content> spp. and <jats:named-content content-type="genus-species">Acinetobacter</jats:named-content> spp. became very important with the increasing prevalence of carbapenem-nonsusceptible clinical isolates. The performance of phenotypic MBL detection methods may depend on the types of MBL and the characteristics of the isolates. A high false-positive rate is a problem with EDTA-based MBL detection methods. We evaluated the performance of double-disk potentiation tests (DDPTs) and disk potentiation tests (DPTs) with dipicolinic acid (DPA) using 44 isolates of <jats:named-content content-type="genus-species">Pseudomonas</jats:named-content> spp. and <jats:named-content content-type="genus-species">Acinetobacter</jats:named-content> spp. producing IMP-1-like, VIM-2-like, and SIM-1 type MBLs. Also, we characterized <jats:named-content content-type="genus-species">P. aeruginosa</jats:named-content> isolates with positive imipenem (IPM)-DPA DDPT, but negative meropenem (MEM)-DPA DDPT, and determined possibility of improving a DDPT by using MacConkey agar. Among five different DDPT methods, the IPM-DPA 250-μg method showed the highest sensitivity (97.7%) and specificity (100%). Among four DPT tests, the highest sensitivity (100%) was shown by the IPM-EDTA 1,900-μg disk method, but the specificity was very low (11.4%). Five of six <jats:named-content content-type="genus-species">P. aeruginosa</jats:named-content> isolates with false-negative DDPTs with MEM-DPA 250-μg disks carried <jats:italic>bla</jats:italic> <jats:sub>IMP-6,</jats:sub> and the high level resistance to MEM (MIC ≥ 512 μg/ml) was reduced by the presence of phenylalanine arginine β-naphtylamide. Improvement of DDPTs was observed when MacConkey agar was used instead of Mueller-Hinton agar. In conclusion, DPA is a better MBL inhibitor than EDTA for detection of <jats:named-content content-type="genus-species">Pseudomonas</jats:named-content> spp. and <jats:named-content content-type="genus-species">Acinetobacter</jats:named-content> spp. with IMP-1-like, VIM-2-like, and SIM-1-type MBLs. In DPA DDPTs, IPM disks perform better than MEM disks when the isolates are highly resistant to MEM due to the overexpression of efflux pumps. </jats:p> |
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| spelling | Yong, Dongeun Lee, Yangsoon Jeong, Seok Hoon Lee, Kyungwon Chong, Yunsop 0095-1137 1098-660X American Society for Microbiology Microbiology (medical) http://dx.doi.org/10.1128/jcm.00818-12 <jats:title>ABSTRACT</jats:title> <jats:p> Accurate detection of metallo-β-lactamase (MBL)-producing <jats:named-content content-type="genus-species">Pseudomonas</jats:named-content> spp. and <jats:named-content content-type="genus-species">Acinetobacter</jats:named-content> spp. became very important with the increasing prevalence of carbapenem-nonsusceptible clinical isolates. The performance of phenotypic MBL detection methods may depend on the types of MBL and the characteristics of the isolates. A high false-positive rate is a problem with EDTA-based MBL detection methods. We evaluated the performance of double-disk potentiation tests (DDPTs) and disk potentiation tests (DPTs) with dipicolinic acid (DPA) using 44 isolates of <jats:named-content content-type="genus-species">Pseudomonas</jats:named-content> spp. and <jats:named-content content-type="genus-species">Acinetobacter</jats:named-content> spp. producing IMP-1-like, VIM-2-like, and SIM-1 type MBLs. Also, we characterized <jats:named-content content-type="genus-species">P. aeruginosa</jats:named-content> isolates with positive imipenem (IPM)-DPA DDPT, but negative meropenem (MEM)-DPA DDPT, and determined possibility of improving a DDPT by using MacConkey agar. Among five different DDPT methods, the IPM-DPA 250-μg method showed the highest sensitivity (97.7%) and specificity (100%). Among four DPT tests, the highest sensitivity (100%) was shown by the IPM-EDTA 1,900-μg disk method, but the specificity was very low (11.4%). Five of six <jats:named-content content-type="genus-species">P. aeruginosa</jats:named-content> isolates with false-negative DDPTs with MEM-DPA 250-μg disks carried <jats:italic>bla</jats:italic> <jats:sub>IMP-6,</jats:sub> and the high level resistance to MEM (MIC ≥ 512 μg/ml) was reduced by the presence of phenylalanine arginine β-naphtylamide. Improvement of DDPTs was observed when MacConkey agar was used instead of Mueller-Hinton agar. In conclusion, DPA is a better MBL inhibitor than EDTA for detection of <jats:named-content content-type="genus-species">Pseudomonas</jats:named-content> spp. and <jats:named-content content-type="genus-species">Acinetobacter</jats:named-content> spp. with IMP-1-like, VIM-2-like, and SIM-1-type MBLs. In DPA DDPTs, IPM disks perform better than MEM disks when the isolates are highly resistant to MEM due to the overexpression of efflux pumps. </jats:p> Evaluation of Double-Disk Potentiation and Disk Potentiation Tests Using Dipicolinic Acid for Detection of Metallo-β-Lactamase-Producing Pseudomonas spp. and Acinetobacter spp Journal of Clinical Microbiology |
| spellingShingle | Yong, Dongeun, Lee, Yangsoon, Jeong, Seok Hoon, Lee, Kyungwon, Chong, Yunsop, Journal of Clinical Microbiology, Evaluation of Double-Disk Potentiation and Disk Potentiation Tests Using Dipicolinic Acid for Detection of Metallo-β-Lactamase-Producing Pseudomonas spp. and Acinetobacter spp, Microbiology (medical) |
| title | Evaluation of Double-Disk Potentiation and Disk Potentiation Tests Using Dipicolinic Acid for Detection of Metallo-β-Lactamase-Producing Pseudomonas spp. and Acinetobacter spp |
| title_full | Evaluation of Double-Disk Potentiation and Disk Potentiation Tests Using Dipicolinic Acid for Detection of Metallo-β-Lactamase-Producing Pseudomonas spp. and Acinetobacter spp |
| title_fullStr | Evaluation of Double-Disk Potentiation and Disk Potentiation Tests Using Dipicolinic Acid for Detection of Metallo-β-Lactamase-Producing Pseudomonas spp. and Acinetobacter spp |
| title_full_unstemmed | Evaluation of Double-Disk Potentiation and Disk Potentiation Tests Using Dipicolinic Acid for Detection of Metallo-β-Lactamase-Producing Pseudomonas spp. and Acinetobacter spp |
| title_short | Evaluation of Double-Disk Potentiation and Disk Potentiation Tests Using Dipicolinic Acid for Detection of Metallo-β-Lactamase-Producing Pseudomonas spp. and Acinetobacter spp |
| title_sort | evaluation of double-disk potentiation and disk potentiation tests using dipicolinic acid for detection of metallo-β-lactamase-producing pseudomonas spp. and acinetobacter spp |
| title_unstemmed | Evaluation of Double-Disk Potentiation and Disk Potentiation Tests Using Dipicolinic Acid for Detection of Metallo-β-Lactamase-Producing Pseudomonas spp. and Acinetobacter spp |
| topic | Microbiology (medical) |
| url | http://dx.doi.org/10.1128/jcm.00818-12 |