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PPE Protein (Rv3873) from DNA Segment RD1 ofMycobacterium tuberculosis: Strong Recognition of Both Specific T-Cell Epitopes and Epitopes Conserved within the PPE Family
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| Zeitschriftentitel: | Infection and Immunity |
|---|---|
| Personen und Körperschaften: | , , , , , , , , |
| In: | Infection and Immunity, 71, 2003, 11, S. 6116-6123 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| author_facet |
Okkels, Limei Meng Brock, Inger Follmann, Frank Agger, Else Marie Arend, Sandra M. Ottenhoff, Tom H. M. Oftung, Fredrik Rosenkrands, Ida Andersen, Peter Okkels, Limei Meng Brock, Inger Follmann, Frank Agger, Else Marie Arend, Sandra M. Ottenhoff, Tom H. M. Oftung, Fredrik Rosenkrands, Ida Andersen, Peter |
|---|---|
| author |
Okkels, Limei Meng Brock, Inger Follmann, Frank Agger, Else Marie Arend, Sandra M. Ottenhoff, Tom H. M. Oftung, Fredrik Rosenkrands, Ida Andersen, Peter |
| spellingShingle |
Okkels, Limei Meng Brock, Inger Follmann, Frank Agger, Else Marie Arend, Sandra M. Ottenhoff, Tom H. M. Oftung, Fredrik Rosenkrands, Ida Andersen, Peter Infection and Immunity PPE Protein (Rv3873) from DNA Segment RD1 ofMycobacterium tuberculosis: Strong Recognition of Both Specific T-Cell Epitopes and Epitopes Conserved within the PPE Family Infectious Diseases Immunology Microbiology Parasitology |
| author_sort |
okkels, limei meng |
| spelling |
Okkels, Limei Meng Brock, Inger Follmann, Frank Agger, Else Marie Arend, Sandra M. Ottenhoff, Tom H. M. Oftung, Fredrik Rosenkrands, Ida Andersen, Peter 0019-9567 1098-5522 American Society for Microbiology Infectious Diseases Immunology Microbiology Parasitology http://dx.doi.org/10.1128/iai.71.11.6116-6123.2003 <jats:title>ABSTRACT</jats:title><jats:p>Proteins encoded by DNA segment RD1 of<jats:italic>Mycobacterium tuberculosis</jats:italic>have recently been demonstrated to play important roles in bacterial virulence, vaccine development, and diagnostic reagent design. Previously, we characterized two immunodominant T-cell antigens, the early secreted antigen target (ESAT-6) and the 10-kDa culture filtrate protein (CFP10), which are encoded by the<jats:italic>esx-lhp</jats:italic>operon in this region. In the present study we characterized a third putative open reading frame in this region,<jats:italic>rv3873</jats:italic>, which encodes a PPE protein. We found that the<jats:italic>rv3873</jats:italic>gene is expressed in<jats:italic>M. tuberculosis</jats:italic>H37Rv and that the native protein, Rv3873, is predominantly associated with the mycobacterial cell or wall. When tested as a His-tagged recombinant protein, Rv3873 stimulated high levels of gamma interferon secretion in peripheral blood mononuclear cells isolated from tuberculosis (TB) patients, as well as from healthy tuberculin purified protein derivative-positive donors. In contrast to other RD1-encoded antigens, Rv3873 was also found to be recognized by a significant proportion of<jats:italic>Mycobacterium bovis</jats:italic>BCG-vaccinated donors. Epitope mapping performed with overlapping peptides revealed a broad pattern of T-cell recognition comprising both TB-specific epitopes and epitopes also recognized by BCG-vaccinated donors. The immunodominant epitope (residues 118 to 135) for both TB patients and BCG-vaccinated individuals was found to be highly conserved among a large number of PPE family members.</jats:p> PPE Protein (Rv3873) from DNA Segment RD1 of<i>Mycobacterium tuberculosis</i>: Strong Recognition of Both Specific T-Cell Epitopes and Epitopes Conserved within the PPE Family Infection and Immunity |
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| title |
PPE Protein (Rv3873) from DNA Segment RD1 ofMycobacterium tuberculosis: Strong Recognition of Both Specific T-Cell Epitopes and Epitopes Conserved within the PPE Family |
| title_unstemmed |
PPE Protein (Rv3873) from DNA Segment RD1 ofMycobacterium tuberculosis: Strong Recognition of Both Specific T-Cell Epitopes and Epitopes Conserved within the PPE Family |
| title_full |
PPE Protein (Rv3873) from DNA Segment RD1 ofMycobacterium tuberculosis: Strong Recognition of Both Specific T-Cell Epitopes and Epitopes Conserved within the PPE Family |
| title_fullStr |
PPE Protein (Rv3873) from DNA Segment RD1 ofMycobacterium tuberculosis: Strong Recognition of Both Specific T-Cell Epitopes and Epitopes Conserved within the PPE Family |
| title_full_unstemmed |
PPE Protein (Rv3873) from DNA Segment RD1 ofMycobacterium tuberculosis: Strong Recognition of Both Specific T-Cell Epitopes and Epitopes Conserved within the PPE Family |
| title_short |
PPE Protein (Rv3873) from DNA Segment RD1 ofMycobacterium tuberculosis: Strong Recognition of Both Specific T-Cell Epitopes and Epitopes Conserved within the PPE Family |
| title_sort |
ppe protein (rv3873) from dna segment rd1 of<i>mycobacterium tuberculosis</i>: strong recognition of both specific t-cell epitopes and epitopes conserved within the ppe family |
| topic |
Infectious Diseases Immunology Microbiology Parasitology |
| url |
http://dx.doi.org/10.1128/iai.71.11.6116-6123.2003 |
| publishDate |
2003 |
| physical |
6116-6123 |
| description |
<jats:title>ABSTRACT</jats:title><jats:p>Proteins encoded by DNA segment RD1 of<jats:italic>Mycobacterium tuberculosis</jats:italic>have recently been demonstrated to play important roles in bacterial virulence, vaccine development, and diagnostic reagent design. Previously, we characterized two immunodominant T-cell antigens, the early secreted antigen target (ESAT-6) and the 10-kDa culture filtrate protein (CFP10), which are encoded by the<jats:italic>esx-lhp</jats:italic>operon in this region. In the present study we characterized a third putative open reading frame in this region,<jats:italic>rv3873</jats:italic>, which encodes a PPE protein. We found that the<jats:italic>rv3873</jats:italic>gene is expressed in<jats:italic>M. tuberculosis</jats:italic>H37Rv and that the native protein, Rv3873, is predominantly associated with the mycobacterial cell or wall. When tested as a His-tagged recombinant protein, Rv3873 stimulated high levels of gamma interferon secretion in peripheral blood mononuclear cells isolated from tuberculosis (TB) patients, as well as from healthy tuberculin purified protein derivative-positive donors. In contrast to other RD1-encoded antigens, Rv3873 was also found to be recognized by a significant proportion of<jats:italic>Mycobacterium bovis</jats:italic>BCG-vaccinated donors. Epitope mapping performed with overlapping peptides revealed a broad pattern of T-cell recognition comprising both TB-specific epitopes and epitopes also recognized by BCG-vaccinated donors. The immunodominant epitope (residues 118 to 135) for both TB patients and BCG-vaccinated individuals was found to be highly conserved among a large number of PPE family members.</jats:p> |
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| author | Okkels, Limei Meng, Brock, Inger, Follmann, Frank, Agger, Else Marie, Arend, Sandra M., Ottenhoff, Tom H. M., Oftung, Fredrik, Rosenkrands, Ida, Andersen, Peter |
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| description | <jats:title>ABSTRACT</jats:title><jats:p>Proteins encoded by DNA segment RD1 of<jats:italic>Mycobacterium tuberculosis</jats:italic>have recently been demonstrated to play important roles in bacterial virulence, vaccine development, and diagnostic reagent design. Previously, we characterized two immunodominant T-cell antigens, the early secreted antigen target (ESAT-6) and the 10-kDa culture filtrate protein (CFP10), which are encoded by the<jats:italic>esx-lhp</jats:italic>operon in this region. In the present study we characterized a third putative open reading frame in this region,<jats:italic>rv3873</jats:italic>, which encodes a PPE protein. We found that the<jats:italic>rv3873</jats:italic>gene is expressed in<jats:italic>M. tuberculosis</jats:italic>H37Rv and that the native protein, Rv3873, is predominantly associated with the mycobacterial cell or wall. When tested as a His-tagged recombinant protein, Rv3873 stimulated high levels of gamma interferon secretion in peripheral blood mononuclear cells isolated from tuberculosis (TB) patients, as well as from healthy tuberculin purified protein derivative-positive donors. In contrast to other RD1-encoded antigens, Rv3873 was also found to be recognized by a significant proportion of<jats:italic>Mycobacterium bovis</jats:italic>BCG-vaccinated donors. Epitope mapping performed with overlapping peptides revealed a broad pattern of T-cell recognition comprising both TB-specific epitopes and epitopes also recognized by BCG-vaccinated donors. The immunodominant epitope (residues 118 to 135) for both TB patients and BCG-vaccinated individuals was found to be highly conserved among a large number of PPE family members.</jats:p> |
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| imprint_str_mv | American Society for Microbiology, 2003 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11, DE-105, DE-14, FID-BBI-DE-23, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
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| spelling | Okkels, Limei Meng Brock, Inger Follmann, Frank Agger, Else Marie Arend, Sandra M. Ottenhoff, Tom H. M. Oftung, Fredrik Rosenkrands, Ida Andersen, Peter 0019-9567 1098-5522 American Society for Microbiology Infectious Diseases Immunology Microbiology Parasitology http://dx.doi.org/10.1128/iai.71.11.6116-6123.2003 <jats:title>ABSTRACT</jats:title><jats:p>Proteins encoded by DNA segment RD1 of<jats:italic>Mycobacterium tuberculosis</jats:italic>have recently been demonstrated to play important roles in bacterial virulence, vaccine development, and diagnostic reagent design. Previously, we characterized two immunodominant T-cell antigens, the early secreted antigen target (ESAT-6) and the 10-kDa culture filtrate protein (CFP10), which are encoded by the<jats:italic>esx-lhp</jats:italic>operon in this region. In the present study we characterized a third putative open reading frame in this region,<jats:italic>rv3873</jats:italic>, which encodes a PPE protein. We found that the<jats:italic>rv3873</jats:italic>gene is expressed in<jats:italic>M. tuberculosis</jats:italic>H37Rv and that the native protein, Rv3873, is predominantly associated with the mycobacterial cell or wall. When tested as a His-tagged recombinant protein, Rv3873 stimulated high levels of gamma interferon secretion in peripheral blood mononuclear cells isolated from tuberculosis (TB) patients, as well as from healthy tuberculin purified protein derivative-positive donors. In contrast to other RD1-encoded antigens, Rv3873 was also found to be recognized by a significant proportion of<jats:italic>Mycobacterium bovis</jats:italic>BCG-vaccinated donors. Epitope mapping performed with overlapping peptides revealed a broad pattern of T-cell recognition comprising both TB-specific epitopes and epitopes also recognized by BCG-vaccinated donors. The immunodominant epitope (residues 118 to 135) for both TB patients and BCG-vaccinated individuals was found to be highly conserved among a large number of PPE family members.</jats:p> PPE Protein (Rv3873) from DNA Segment RD1 of<i>Mycobacterium tuberculosis</i>: Strong Recognition of Both Specific T-Cell Epitopes and Epitopes Conserved within the PPE Family Infection and Immunity |
| spellingShingle | Okkels, Limei Meng, Brock, Inger, Follmann, Frank, Agger, Else Marie, Arend, Sandra M., Ottenhoff, Tom H. M., Oftung, Fredrik, Rosenkrands, Ida, Andersen, Peter, Infection and Immunity, PPE Protein (Rv3873) from DNA Segment RD1 ofMycobacterium tuberculosis: Strong Recognition of Both Specific T-Cell Epitopes and Epitopes Conserved within the PPE Family, Infectious Diseases, Immunology, Microbiology, Parasitology |
| title | PPE Protein (Rv3873) from DNA Segment RD1 ofMycobacterium tuberculosis: Strong Recognition of Both Specific T-Cell Epitopes and Epitopes Conserved within the PPE Family |
| title_full | PPE Protein (Rv3873) from DNA Segment RD1 ofMycobacterium tuberculosis: Strong Recognition of Both Specific T-Cell Epitopes and Epitopes Conserved within the PPE Family |
| title_fullStr | PPE Protein (Rv3873) from DNA Segment RD1 ofMycobacterium tuberculosis: Strong Recognition of Both Specific T-Cell Epitopes and Epitopes Conserved within the PPE Family |
| title_full_unstemmed | PPE Protein (Rv3873) from DNA Segment RD1 ofMycobacterium tuberculosis: Strong Recognition of Both Specific T-Cell Epitopes and Epitopes Conserved within the PPE Family |
| title_short | PPE Protein (Rv3873) from DNA Segment RD1 ofMycobacterium tuberculosis: Strong Recognition of Both Specific T-Cell Epitopes and Epitopes Conserved within the PPE Family |
| title_sort | ppe protein (rv3873) from dna segment rd1 of<i>mycobacterium tuberculosis</i>: strong recognition of both specific t-cell epitopes and epitopes conserved within the ppe family |
| title_unstemmed | PPE Protein (Rv3873) from DNA Segment RD1 ofMycobacterium tuberculosis: Strong Recognition of Both Specific T-Cell Epitopes and Epitopes Conserved within the PPE Family |
| topic | Infectious Diseases, Immunology, Microbiology, Parasitology |
| url | http://dx.doi.org/10.1128/iai.71.11.6116-6123.2003 |